This article reviews the progress of a personal endeavour to develop chromatography as a quantitative procedure for the determination of reaction stoichiometries and equilibrium constants governing protein interactions. As well as affording insight into an aspect of chromatography with which many protein chemists are unfamiliar, it shows the way in which minor adaptations of conventional chromatographic practices have rendered the technique one of the most powerful methods available for the characterization of interactions. That pathway towards quantification is followed from the introduction of frontal gel filtration for the study of protein self-association to the characterization of ligand binding by the biosensor variant of quantitative affinity chromatography.
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October 2003
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Conference Article|
October 01 2003
The development of chromatography for the characterization of protein interactions: a personal perspective
D.J. Winzor
D.J. Winzor
1
Department of Biochemistry, University of Queensland, Brisbane, Queensland 4072, Australia
1e-mail d.winzor@mailbox.uq.edu.au
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Publisher: Portland Press Ltd
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2003 Biochemical Society
2003
Biochem Soc Trans (2003) 31 (5): 1010–1014.
Citation
D.J. Winzor; The development of chromatography for the characterization of protein interactions: a personal perspective. Biochem Soc Trans 1 October 2003; 31 (5): 1010–1014. doi: https://doi.org/10.1042/bst0311010
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