The Wilson's disease protein (WNDP), a copper transporter, is a crucial mediator of copper homoeostasis in mammalian cells. We recently found that changes in copper concentration regulate the phosphorylation level of WNDP. WNDP phosphorylation was observed in several mammalian cell lines, suggesting that a common phosphorylation pathway exists in these cells. Here we demonstrate that WNDP expressed in Sf9 insect cells is also phosphorylated, as evidenced by metabolic labelling of these cells with [32P]Pi. Because the baculovirus system allows us to generate large amounts of protein, we are using this expression method to isolate WNDP and map the sites of WNDP phosphorylation. The identification of phosphorylation sites is the first step towards understanding the physiological role of WNDP phosphorylation.
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August 2002
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Conference Article|
August 01 2002
The Wilson's disease protein expressed in Sf9 cells is phosphorylated
S. M. Vanderwerf;
S. M. Vanderwerf
1Department of Biochemistry and Molecular Biology, Oregon Health & Science University, Portland, OR 97201, U.S.A.
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S. Lutsenko
S. Lutsenko
1
1Department of Biochemistry and Molecular Biology, Oregon Health & Science University, Portland, OR 97201, U.S.A.
1 To whom correspondence should be addressed (e-mail lutsenko@ohsu.edu)
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Publisher: Portland Press Ltd
Received:
March 12 2002
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (4): 739–741.
Article history
Received:
March 12 2002
Citation
S. M. Vanderwerf, S. Lutsenko; The Wilson's disease protein expressed in Sf9 cells is phosphorylated. Biochem Soc Trans 1 August 2002; 30 (4): 739–741. doi: https://doi.org/10.1042/bst0300739
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