14-3-3 proteins interact with a novel phosphothreonine motif (Y946pTV) at the extreme C-terminal end of the plant plasma membrane H+-ATPase molecule. Phosphorylation-independent binding of 14-3-3 protein to the YTV motif can be induced by the fungal phytotoxin fusicoccin. The molecular basis for the phosphorylation-independent interaction between 14-3-3 and H+-ATPase in the presence of fusicoccin has been investigated in more detail. Fusicoccin binds to a heteromeric receptor that involves both 14-3-3 protein and H+-ATPase. Binding of fusicoccin is dependent upon the YTV motif in the H+-ATPase and, in addition, requires residues further upstream of this motif. Apparently, 14-3-3 proteins interact with the unusual epitope in H+-ATPase via its conserved amphipathic groove. This implies that very diverse epitopes bind to a common structure in the 14-3-3 protein.
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August 2002
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Conference Article|
August 01 2002
Phosphorylation-independent interaction between 14-3-3 protein and the plant plasma membrane H+-ATPase
J. Borch;
J. Borch
*Department of Plant Biology, Plant Physiology and Anatomy Laboratory, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871-Frederiksberg C, Copenhagen, Denmark
†Department of Biochemistry and Molecular Biology, Odense University Campus, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
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K. Bych;
K. Bych
*Department of Plant Biology, Plant Physiology and Anatomy Laboratory, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871-Frederiksberg C, Copenhagen, Denmark
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P. Roepstorff;
P. Roepstorff
†Department of Biochemistry and Molecular Biology, Odense University Campus, University of Southern Denmark, Campusvej 55, DK 5230 Odense M, Denmark
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M. G. Palmgren;
M. G. Palmgren
*Department of Plant Biology, Plant Physiology and Anatomy Laboratory, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871-Frederiksberg C, Copenhagen, Denmark
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A. T. Fuglsang
A. T. Fuglsang
1
*Department of Plant Biology, Plant Physiology and Anatomy Laboratory, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871-Frederiksberg C, Copenhagen, Denmark
1To whom correspondence should be addressed (e-mail atf@kvl.dk)
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Publisher: Portland Press Ltd
Received:
March 20 2002
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (4): 411–415.
Article history
Received:
March 20 2002
Citation
J. Borch, K. Bych, P. Roepstorff, M. G. Palmgren, A. T. Fuglsang; Phosphorylation-independent interaction between 14-3-3 protein and the plant plasma membrane H+-ATPase. Biochem Soc Trans 1 August 2002; 30 (4): 411–415. doi: https://doi.org/10.1042/bst0300411
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