14-3-3 proteins are involved in signalling processes in neuronal cells. Using isoform-specific antibodies we have examined the variation in 14-3-3 isoform neurolocation in normal and scrapie-infected murine brain and show that in defined areas of the brain there are significant changes associated with the pathology of the disease process. The appearance of 14-3-3 proteins in the cerebrospinal fluid (CSF) is a consequence of neuronal disease and the detection of specific isoforms of the 14-3-3 proteins in the CSF is characteristic of some neurodegenerative diseases. In this study, monitoring specifically for theγ 14-3-3 isoform in the CSF by both Western-blot analysis and ELISA we can show a level of correlation between the assays.
Specific 14-3-3 isoform detection and immunolocalization in prion diseases
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H. C. Baxter, J. R. Fraser, W.-G. Liu, J. L. Forster, S. Clokie, P. Steinacker, M. Otto, E. Bahn, J. Wiltfang, A. Aitken; Specific 14-3-3 isoform detection and immunolocalization in prion diseases. Biochem Soc Trans 1 August 2002; 30 (4): 387–391. doi: https://doi.org/10.1042/bst0300387
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