The plant cell wall is a complex structure consisting of a variety of polymers including cellulose, xyloglucan, xylan and polygalacturonan. Biochemical and genetic analysis has made it possible to clone genes encoding cellulose synthases (CesA). A comparison of the predicted protein sequences in the Arabidopsis genome indicates that 30 divergent genes with similarity to CesAs exist. It is possible that these cellulose synthase-like (Csl) proteins do not contribute to cellulose synthesis, but rather to the synthesis of other wall polymers. A major challenge is, therefore, to assign biological function to these genes. In an effort to address this issue we have systematically identified T-DNA or transposon insertions in 17 Arabidopsis Csls. Phenotypic characterization of ‘knock-out’ mutants includes the determination of spectroscopic profile differences in mutant cell walls from wild-type plants by Fourier-transform IR microscopy. A more precise characterization includes cell wall fractionation followed by neutral sugar composition analysis by anionic exchange chromatography.
Skip Nav Destination
Article navigation
April 2002
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Conference Article|
April 01 2002
Genetic dissection of plant cell-wall biosynthesis
D. T. Bonetta;
D. T. Bonetta
1Carnegie Institution of Washington, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, U.S.A.
Search for other works by this author on:
M. Facette;
M. Facette
1Carnegie Institution of Washington, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, U.S.A.
Search for other works by this author on:
T. K. Raab;
T. K. Raab
1Carnegie Institution of Washington, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, U.S.A.
Search for other works by this author on:
C. R. Somerville
C. R. Somerville
1
1Carnegie Institution of Washington, Department of Plant Biology, 260 Panama Street, Stanford, CA 94305, U.S.A.
1To whom correspondence should be addressed (e-mail crs@andrew2.stanford.edu).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
December 19 2001
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (2): 298–301.
Article history
Received:
December 19 2001
Citation
D. T. Bonetta, M. Facette, T. K. Raab, C. R. Somerville; Genetic dissection of plant cell-wall biosynthesis. Biochem Soc Trans 1 April 2002; 30 (2): 298–301. doi: https://doi.org/10.1042/bst0300298
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.