Translation initiation in eukaryotes is a complicated process involving some of the largest cellular structures, the ribosomes, together with approx. 11 initiation factors, and a poorly characterized set of other proteins. The concerted action of all these components ultimately results in the formation of an 80 S ribosomal complex on the AUG codon of an mRNA, which is competent to start polypeptide production. In this brief overview, we describe the strategies developed by our laboratory to apply surface plasmon resonance (SPR)-based technology to the problem of elucidating kinetic aspects of substeps within the translation-initiation reaction. We then review how other groups have used similar SPR-based techniques to study related interactions.
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Conference Article|
April 01 2002
Translation initiation and surface plasmon resonance: new technology applied to old questions
T. von der Haar;
T. von der Haar
1Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, Manchester M60 IQD, U.K.
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J. M. X. Hughes;
J. M. X. Hughes
1Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, Manchester M60 IQD, U.K.
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M. Manjurul Karim;
M. Manjurul Karim
1Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, Manchester M60 IQD, U.K.
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M. Ptushkina;
M. Ptushkina
1Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, Manchester M60 IQD, U.K.
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J. E. G. McCarthy
J. E. G. McCarthy
1
1Posttranscriptional Control Group, Department of Biomolecular Sciences, UMIST, Manchester M60 IQD, U.K.
1To whom correspondence should be addressed (e-mail John.McCarthy@umist.ac.uk).
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Publisher: Portland Press Ltd
Received:
December 06 2001
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2002 Biochemical Society
2002
Biochem Soc Trans (2002) 30 (2): 155–162.
Article history
Received:
December 06 2001
Citation
T. von der Haar, J. M. X. Hughes, M. Manjurul Karim, M. Ptushkina, J. E. G. McCarthy; Translation initiation and surface plasmon resonance: new technology applied to old questions. Biochem Soc Trans 1 April 2002; 30 (2): 155–162. doi: https://doi.org/10.1042/bst0300155
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