The cellular response to DNA-damaging agents is partly mediated by DNA-binding transcription factors such as p53 and nuclear factor κB (NF-κB). Typically NF-κB activation is associated with resistance to apoptosis. Following stimulation with UV light however, NF-κB activation has been shown to be required for programmed cell death. To study this effect further and to determine the relationship between NF-κB and p53 function, we have examined the effect of UV light on U2OS cells. UV stimulation resulted in the activation of NF-κB DNA-binding and the induction of p53. Surprisingly, and in contrast with tumour necrosis factor α stimulation, this UV-induced NF-κB was transcriptionally inert. These observations suggest a model in which the NF-κB switch from an anti-apoptotic to a pro-apoptotic role within the cell results from modulation of its ability to stimulate gene expression, possibly as a result of the ability of p53 to sequester transcriptional co-activator proteins such as p300/CREB (cAMP-response-element-binding protein)-binding protein.
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Conference Article|
November 01 2001
UV stimulation induces nuclear factor κB (NF-κB) DNA-binding activity but not transcriptional activation
K. J. Campbell;
K. J. Campbell
1Division of Gene Regulation and Expression, Wellcome Trust Biocentre, University of Dundee, Dundee DDI 5EH, Scotland, U.K.
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N. R. Chapman;
N. R. Chapman
1Division of Gene Regulation and Expression, Wellcome Trust Biocentre, University of Dundee, Dundee DDI 5EH, Scotland, U.K.
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N. D. Perkins
N. D. Perkins
1
1Division of Gene Regulation and Expression, Wellcome Trust Biocentre, University of Dundee, Dundee DDI 5EH, Scotland, U.K.
1To whom correspondence should be addressed (e-mail n.d.perkins@dundee.ac.uk).
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Publisher: Portland Press Ltd
Received:
May 30 2001
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2001 Biochemical Society
2001
Biochem Soc Trans (2001) 29 (6): 688–691.
Article history
Received:
May 30 2001
Citation
K. J. Campbell, N. R. Chapman, N. D. Perkins; UV stimulation induces nuclear factor κB (NF-κB) DNA-binding activity but not transcriptional activation. Biochem Soc Trans 1 November 2001; 29 (6): 688–691. doi: https://doi.org/10.1042/bst0290688
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