We are using a molecular-genetic approach to investigate the role of nuclear genes in the biogenesis of the electron transfer complexes of mitochondria and chloroplasts. Our analysis of nuclear mutants of the green alga Chlamydomonas that are defective in respiration or photosynthesis has led to the identification of genes encoding factors required for the expression of specific organellar genes, and genes encoding structural components of the complexes.

Keywords:
insertional mutagenesis, photosynthesis, respiration, PPR, pentatricopeptide repeat, TTC, 2,3,5-triphenytetrazolium chloride
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© 2001 Biochemical Society
2001
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