Liver microsomes contain two carnitine acyltransferase activities. One of these has properties closely corresponding to those of 88 kDa mitochondrial carnitine palmitoyltransferase-1 (CPT-1). Antisera against CPT-1 cross-react with an 88 kDa microsomal protein, suggesting that CPT-1 may be targeted to both microsomal and mitochondrial membranes. However, no experiments using cDNAs corresponding to CPT-1 involving in vitro translation with microsomes or involving in vivo COS-1 cell transfection provided any evidence to support this hypothesis.
Keywords:
CAT, carnitine acyltransferase,
CPT I and CPT II, the overt and latent forms respectively of carnitine palmitoyltransferase (EC 2.3.1.21),
EGFP, enhanced green fluorescent protein,
etomoxir, 2-[6-(4-chlorophenoxy)hexyl]oxirane carboxylic acid,
N2ph, 2,4-dinitrophenyl,
microsomes,
mitochondria,
protein targeting
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© 2001 Biochemical Society
2001
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