Different forms of phospholipase D (dependent on and independent of the presence of phospha-tidylinositol 4,5-bisphosphate, PIP2) were identified in maturing and germinating seeds of Brassica napus. Both forms were present in cytosolic and membrane fractions of maturing seeds. PIP2-dependent activity increased continuously during seed germination, while PIP2-independent activity appeared mostly at the very beginning of seed maturation. PIP2-dependent activity was detected mainly in the plasma-membrane fraction. Phosphatidylinositol-specific phospholipase C (PI-PLC) was found only in membrane fractions of both types of developing rape seed tissues. The increasing activities of PLC and PIP2-dependent PLD were mainly detected in hypocotyls of seedlings. Some biochemical characteristics of both described enzymes are also presented.
Skip Nav Destination
Article navigation
December 2000
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Conference Article|
December 01 2000
Study of phospholipases D and C in maturing and germinating seeds of Brassica napus
Z. Novotna;
Z. Novotna
*Institute of Chemical Technology Prague, Technicka 3, 166 28 Prague 6, Czech Republic
Search for other works by this author on:
O. Valentova;
O. Valentova
1
*Institute of Chemical Technology Prague, Technicka 3, 166 28 Prague 6, Czech Republic
1To whom correspondence should be addressed (e-mail olga.valentova@vscht.cz)
Search for other works by this author on:
J. Martinec;
J. Martinec
†Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 135, 165 02 Prague 6, Czech Republic
Search for other works by this author on:
T. Feltl;
T. Feltl
†Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojova 135, 165 02 Prague 6, Czech Republic
Search for other works by this author on:
K. Nokhrina
K. Nokhrina
‡Institute of Plant Physiology and Genetics, Academy of Sciences of Ukraine, 03022 Kiev, Ukraine
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
June 27 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 817–818.
Article history
Received:
June 27 2000
Citation
Z. Novotna, O. Valentova, J. Martinec, T. Feltl, K. Nokhrina; Study of phospholipases D and C in maturing and germinating seeds of Brassica napus. Biochem Soc Trans 1 December 2000; 28 (6): 817–818. doi: https://doi.org/10.1042/bst0280817
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.