Fluorescent phosphatidic acid and phosphatidylcholine were used to characterize lipid-transport pathways into lipid bodies in an oleaginous fungus, Mortierella ramanniana var. angulispora. Several characteristics of the lipid transport such as temperature dependence and ATP dependence were evaluated. The transport depicted by these fluorescent lipids was consistent with metabolism of radiolabelled lipids, indicating that fluorescent lipids are useful to study lipid-body formation in this fungus. The results dissect lipid transport of phosphatidic acid and phosphatidylcholine into lipid bodies and reveal regulatory steps for lipidbody formation in this fungus.
Keywords:
lipid transport,
oleaginous fungi,
triacylglycerol biosynthesis,
BODIPY, boron dipyrromethene difluoride,
C5-DMB-PA, 1-palmitoyl-2-[5-(5,7-dimethyl BODIPY)-1-penta-noyl]-phosphatidic acid,
C5-DMB-PC, 1-palmitoyl-2-[5-(5,7-di-methyl BODIPY)-1-pentanol]-phosphatidylcholine,
PA, phosphatidic acid,
PC, phosphatidylcholine,
TAG, triacylglycerol
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© 2000 Biochemical Society
2000
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