We have characterized three CoA-independent types of enzyme, phospholipases, phospholipid: diacylglycerol acyltransferases (PDATs) and cholinephosphotransferases, responsible for the removal of unusual fatty acids from phosphatidylcholine (PC) in microsomal preparations from developing oil seeds. The metabolism of sn-2-[14C]acyl-PC was monitored in microsomal preparations from various oilseeds having either medium-chain, acetylenic, epoxy or hydroxy fatty acids as their major fatty acids in the oil. The results indicate that PDAT plays a major role in removing ricinoleic acid and vernolic acid from phospholipids in Ricinus communis and Crepis palaestina seeds, respectively. However, vernolic, crepenynic and capric acids are primarily removed from phospholipids by phospholipases in Euphorbia lagascae, Crepis rubra and elm seeds, respectively. Further, we show that significant PDAT activity is also present in vegetative tissues of Arabidopsis thaliana.
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Conference Article|
December 01 2000
The involvement of phospholipid:diacylglycerol acyltransferases in triacylglycerol production
A. Banaś;
A. Banaś
1
*Scandinavian Biotechnology Research AB, SE-268 31 Svalöv, Sweden
1To whom correspondence should be addressed (e-mail antoni.banas@w.slu.se)
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A. Dahlqvist;
A. Dahlqvist
*Scandinavian Biotechnology Research AB, SE-268 31 Svalöv, Sweden
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U. Ståhl;
U. Ståhl
†Scandinavian Biotechnology Research AB, SLU, Genetic Center, Box 7080, SE-270 07 Uppsala, Sweden
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M. Lenman;
M. Lenman
*Scandinavian Biotechnology Research AB, SE-268 31 Svalöv, Sweden
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S. Stymne
S. Stymne
‡Department of Plant Breeding Research, Swedish University of Agricultural Sciences, SE-268 31 Svalöv, Sweden
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Publisher: Portland Press Ltd
Received:
June 26 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 703–705.
Article history
Received:
June 26 2000
Citation
A. Banaś, A. Dahlqvist, U. Ståhl, M. Lenman, S. Stymne; The involvement of phospholipid:diacylglycerol acyltransferases in triacylglycerol production. Biochem Soc Trans 1 December 2000; 28 (6): 703–705. doi: https://doi.org/10.1042/bst0280703
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