Deficiencies in the activity of the microsomal oleoyl phosphatidylcholine (oleoyl-PC) desaturase from peanuts are the basis of the high oleate oil. Mutation of aspartate-150 to asparagine and the attendant decrease in activity, together with the loss in expression of the higher activity transcript, was the molecular basis of the high oleate trait. The ability of oleoyl-PC desaturase to desaturate palmitoleate, oleate and 10(Z) nonadecenoate to methylene-interrupted products was not consistent with description of this activity as a Δ12 or ω-6 desaturase. Electrospray MS was used to examine the intact phospholipid products of desaturation by the oleoyl-PC desaturase. PC and phosphatidylinositol containing unsaturated moieties could be desaturated. The enzyme can act on either sn-1 or sn-2 moieties. Phosphatidylethanolamine was a poor substrate.
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Conference Article|
December 01 2000
Molecular properties of the oleoyl-phosphatidylcholine desaturase from Arachis hypogaea L.
G. L. Powell;
G. L. Powell
1
1Department of Biological Sciences, Clemson University, Clemson, SC 29634-0326, U.S.A.
1To whom correspondence should be addressed (e-mail glpwl@clemson.edu)
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S. Jung;
S. Jung
1Department of Biological Sciences, Clemson University, Clemson, SC 29634-0326, U.S.A.
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A. C. Bruner;
A. C. Bruner
1Department of Biological Sciences, Clemson University, Clemson, SC 29634-0326, U.S.A.
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J. L. Swartzbeck;
J. L. Swartzbeck
1Department of Biological Sciences, Clemson University, Clemson, SC 29634-0326, U.S.A.
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A. G. Abbott
A. G. Abbott
1Department of Biological Sciences, Clemson University, Clemson, SC 29634-0326, U.S.A.
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Publisher: Portland Press Ltd
Received:
June 26 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 625–627.
Article history
Received:
June 26 2000
Citation
G. L. Powell, S. Jung, A. C. Bruner, J. L. Swartzbeck, A. G. Abbott; Molecular properties of the oleoyl-phosphatidylcholine desaturase from Arachis hypogaea L.. Biochem Soc Trans 1 December 2000; 28 (6): 625–627. doi: https://doi.org/10.1042/bst0280625
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