Currently available techniques for the separation and characterization of different glycerolipids are complicated and/or time consuming. By modulating the stationary phase in a solid-phase extraction (SPE) manifold, efficient and rapid separation of plant membrane lipids was achieved. The glycerolipids from rice and maize tissues were separated into seven classes (monogalactosyldiacylglycerol, digalactosyldiacylglycerol, phosphatidylethanolamine, phosphatidylcholine, sulphoquinovosyldiacylglycerol, phosphatidylinositol and phosphatidylglycerol). The pigments present in the rice and maize leaves and rice stems were successfully removed from the total lipid extracts. Pigment-free plant tissue (rice roots) was also analysed. The fatty acid profile of each lipid class isolated by SPE agreed well with those obtained by other separation techniques. The recovery of glycerolipids was at least 87°.
Skip Nav Destination
Article navigation
December 2000
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Conference Article|
December 01 2000
Determination of glycerolipid composition of rice and maize tissues using solid-phase extraction
I. Rizov;
I. Rizov
1
1Laboratory of Molecular Biology and Biotechnology, Mediterranean Agronomic Institute of Chania, 73100 Chania, Greece
1To whom correspondence should be addressed (e-mail ivzizov@hotmail.com)
Search for other works by this author on:
A. Doulis
A. Doulis
1Laboratory of Molecular Biology and Biotechnology, Mediterranean Agronomic Institute of Chania, 73100 Chania, Greece
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
July 03 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (6): 586–589.
Article history
Received:
July 03 2000
Citation
I. Rizov, A. Doulis; Determination of glycerolipid composition of rice and maize tissues using solid-phase extraction. Biochem Soc Trans 1 December 2000; 28 (6): 586–589. doi: https://doi.org/10.1042/bst0280586
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.