In plant cells, the organization of the Golgi apparatus and its interrelationships with the endoplasmic reticulum differ from those in mammalian and yeast cells. Endoplasmic reticulum and Golgi apparatus can now be visualized in plant cells in vivo with green fluorescent protein (GFP) specifically directed to these compartments. This makes it possible to study the dynamics of the membrane transport between these two organelles in the living cells. The GFP approach, in conjunction with a considerable volume of data about proteins participating in the transport between endoplasmic reticulum and Golgi in yeast and mammalian cells and the identification of their putative plant homologues, should allow the establishment of an experimental model in which to test the involvement of the candidate proteins in plants. As a first step towards the development of such a system, we are using Sar1, a small G-protein necessary for vesicle budding from the endoplasmic reticulum. This work has demonstrated that the introduction of Sar1 mutants blocks the transport from endoplasmic reticulum to Golgi in vivo in tobacco leaf epidermal cells and has therefore confirmed the feasibility of this approach to test the function of other proteins that are presumably involved in this step of endo-membrane trafficking in plant cells.
Skip Nav Destination
Article navigation
August 2000
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkAdvertising
Conference Article|
August 01 2000
Organization of transport from endoplasmic reticulum to Golgi in higher plants
A. V. Andreeva;
A. V. Andreeva
1
1Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Headington, Oxford OX3 0BP, U.K.
1To whom correspondence should be addressed (e-mail aandreeva@brookes.ac.uk)
Search for other works by this author on:
H. Zheng;
H. Zheng
1Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Headington, Oxford OX3 0BP, U.K.
Search for other works by this author on:
C. M. Saint-Jore;
C. M. Saint-Jore
1Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Headington, Oxford OX3 0BP, U.K.
Search for other works by this author on:
M. A. Kutuzov;
M. A. Kutuzov
1Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Headington, Oxford OX3 0BP, U.K.
Search for other works by this author on:
D. E. Evans;
D. E. Evans
1Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Headington, Oxford OX3 0BP, U.K.
Search for other works by this author on:
C. R. Hawes
C. R. Hawes
1Research School of Biological and Molecular Sciences, Oxford Brookes University, Gipsy Lane Campus, Headington, Oxford OX3 0BP, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 02 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (4): 505–512.
Article history
Received:
March 02 2000
Citation
A. V. Andreeva, H. Zheng, C. M. Saint-Jore, M. A. Kutuzov, D. E. Evans, C. R. Hawes; Organization of transport from endoplasmic reticulum to Golgi in higher plants. Biochem Soc Trans 1 August 2000; 28 (4): 505–512. doi: https://doi.org/10.1042/bst0280505
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Captcha Validation Error. Please try again.