Gene regulation involves the generation of a local chromatin topology that is conducive to transcription. Several classes of chromatin remodelling activity have been shown to play a role in this process. ATP-dependent chromatin-remodelling activities use energy derived from the hydrolysis of ATP to alter the structure of chromatin, making it more accessible for transcription factor binding. The yeast SWI-SWF complex is the founding member of this family of ATP-dependent chromatin-remodelling activities. We have developed a model system to study the ability of the SWI-SWF complex to alter chromatin structure. Using this system, we find that SWI-SWF is able to alter the position of nucleosomes along the DNA. This is consistent with recent reports that other ATP-dependent chromatin-remodelling activities can alter the positions of nucleosomes along DNA. This suggests that nucleosome mobilization may be a general feature of the activity of ATP-dependent chromatin-remodelling activities. Some of the mechanisms by which nucleosomes may be moved along DNA are discussed.
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Conference Article|
August 01 2000
Mechanisms for ATP-dependent chromatin remodelling
I. Whitehouse;
I. Whitehouse
1Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
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A. Flaus;
A. Flaus
1Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
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K. Havas;
K. Havas
1Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
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T. Owen-Hughes
T. Owen-Hughes
1
1Division of Gene Regulation and Expression, The Wellcome Trust Biocentre, Department of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, U.K.
1To whom correspondence should be addressed (e-mail ta.owenhughes@dundee.ac.uk)
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Publisher: Portland Press Ltd
Received:
March 01 2000
Online ISSN: 1470-8752
Print ISSN: 0300-5127
© 2000 Biochemical Society
2000
Biochem Soc Trans (2000) 28 (4): 376–379.
Article history
Received:
March 01 2000
Citation
I. Whitehouse, A. Flaus, K. Havas, T. Owen-Hughes; Mechanisms for ATP-dependent chromatin remodelling. Biochem Soc Trans 1 August 2000; 28 (4): 376–379. doi: https://doi.org/10.1042/bst0280376
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