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Keywords: surface plasmon resonance
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Biochem J (2021) 478 (11): 2145–2161.
Published: 11 June 2021
...–peptide interactions SET1/MLL proteins surface plasmon resonance WD40 repeat protein 5 (WDR5) is a conserved chromatin-associated protein that is involved in a number of transient protein–protein interactions [ 1 ]. However, WDR5 is notoriously known for its regulatory role in multisubunit...
Includes: Supplementary data
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Biochem J (2017) 474 (21): 3675–3687.
Published: 23 October 2017
... provide kinetic and affinity data for interaction between AtPHR1 and P1BS sites. Using surface plasmon resonance, a tandem P1BS sequence showed ∼50-fold higher affinity for MBPAtdPHR1 (a fusion protein comprising the DNA-binding domain and coiled-coil domain of AtPHR1 fused to maltose-binding protein...
Includes: Supplementary data
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Biochem J (2017) 474 (4): 539–556.
Published: 03 February 2017
...–β2 loop and that it binds with somewhat higher affinity to PtdIns(3,4,5)P 3 compared with PtdIns(4,5)P 2 . Surface plasmon resonance (SPR) with lipid headgroups immobilised and the PH domain as an analyte indicate affinities of 300 µM for PtdIns(3,4,5)P 3 and 1 mM for PtdIns(4,5)P 2 . In contrast...
Includes: Supplementary data
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Biochem J (2016) 473 (17): 2645–2654.
Published: 30 August 2016
... dissociation rate k d resulting in an overall 12-fold decreased binding affinity in surface plasmon resonance (SPR) experiments. The delayed dissociation might provide BoNT/A more time for endocytosis into synaptic vesicles. In conclusion, we show the importance of the complex N559-glycan of SV2C-LD4, adding...
Includes: Supplementary data
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Biochem J (2015) 465 (3): 443–457.
Published: 22 January 2015
... IR and surface plasmon resonance studies also indicated different modes of interaction for LL-37 and RL-37 with anionic and neutral membranes. This correlated with a distinctly different mode of bacterial membrane permeabilization, as determined using a flow cytometric method involving impermeant...
Includes: Supplementary data
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Biochem J (2014) 457 (1): 137–149.
Published: 10 December 2013
... in the extracellular matrix, which could provide new insights into its biological roles. We identified 17 new partners of PCPE-1 by SPR (surface plasmon resonance) imaging. PCPE-1 forms a transient complex with the β-amyloid peptide, whereas it forms high or very high affinity complexes with laminin-111 ( K D =58.8 pM...
Includes: Supplementary data
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Biochem J (2012) 444 (3): 417–428.
Published: 29 May 2012
... glycosaminoglycan composed of sulfated S-domains and unsulfated NA-domains. To elucidate the molecular mechanism of binding of FH to glycosaminoglycans, we performed ultracentrifugation, X-ray scattering and surface plasmon resonance with FH and glycosaminoglycan fragments. Ultracentrifugation showed that FH formed...
Includes: Supplementary data
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Biochem J (2011) 438 (2): 291–301.
Published: 12 August 2011
... identified in the protein, thus making it a potential new member of the BH3-only protein family. In the present study, we provide NMR, SPR (surface plasmon resonance) and crystallographic evidence that a peptide spanning residues 147–172 in SOUL interacts with the anti-apoptotic protein Bcl-xL. We have...
Includes: Supplementary data
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Biochem J (2009) 419 (3): 577–584.
Published: 14 April 2009
... against PCSK9. Studies using surface plasmon resonance demonstrated that direct binding of PCSK9 to the LDLR could be abolished with three different anti-PCSK9 antibodies. Two of these antibodies were raised against peptide epitopes in a region of the catalytic domain of PCSK9 that is involved...
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Biochem J (2009) 419 (1): 221–230.
Published: 13 March 2009
... quantitative DNA-binding study of this plant-specific family of proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics...
Includes: Supplementary data
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Biochem J (2005) 391 (3): 659–666.
Published: 25 October 2005
... for rapid and sensitive in vitro assays. Here, we present new automatized bioassays to detect VAMP cleavage by BoNT/B and F. Western blotting and SPR (surface plasmon resonance) methods revealed that BoNT/B and F totally cleave their substrate on immunoisolated SVs (synaptic vesicles). Real-time monitoring...
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Biochem J (2005) 390 (1): 177–188.
Published: 09 August 2005
... and surface plasmon resonance studies with model phosphatidylcholine/cholesterol membranes. The mode of interaction changes radically for a peptide with a stable, preformed helical conformation compared with others that form a structure only on membrane binding. The close correlation between effects observed...
Includes: Supplementary data
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Biochem J (2004) 381 (3): 725–733.
Published: 27 July 2004
...Mili KAPOOR; P. L. Swarna MUKHI; Namita SUROLIA; K. SUGUNA; Avadhesha SUROLIA The binding of enoyl-ACP (acyl-carrier protein) reductase from Plasmodium falciparum (PfENR) with its substrates and inhibitors has been analysed by SPR (surface plasmon resonance). The binding of the substrate analogue...
Includes: Supplementary data
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Biochem J (2003) 375 (3): 623–631.
Published: 01 November 2003
... techniques. Surface plasmon resonance analysis indicated a high affinity in the interaction between eIF2β and CK2α, whereas the affinity for the association with CK2β is much lower. Free CK2α is unable to phosphorylate eIF2β, whereas up to 1.2 mol of phosphate/mol of eIF2β was incorporated...
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Biochem J (2003) 373 (1): 115–123.
Published: 01 July 2003
... inhibition of cytochrome c reduction by flavocytochrome b 2 , competitive ELISA tests and surface plasmon resonance. We have thus defined a minimal epitope surface on the haem domain; it encompasses positions 63, 64, 65, 67, 69 and 70 and one or both haem propionates. When the haem and flavin domains...
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Biochem J (2002) 368 (1): 111–119.
Published: 15 November 2002
... to homogeneous glycoclusters. The thiol group of the cysteine residue was used to tag the glycosylated oligolysines upon reaction with fluorescein iodoacetamide. The affinity of these glycoclusters towards two plant lectins was assessed by surface plasmon resonance. The selectivity of their cell uptake...
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Biochem J (2002) 364 (1): 173–180.
Published: 08 May 2002
... the T-antigen disaccharide Galβ1,3GalNAc, lactose and galactose, but also against mannose and oligomannosides. Biochemical analyses based on surface-plasmon-resonance measurements, combined with the X-ray-crystallographic determination of the structure of a jacalin—α-methyl-mannose complex at 2Å...
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Biochem J (2001) 359 (3): 679–685.
Published: 25 October 2001
... the mechanisms by which Ca 2+ mediates the membrane binding of C2 domains, we measured the membrane binding of the C2 domains of group IV cytosolic phospholipase A 2 (cPLA 2 ) and protein kinase C-α (PKC-α) by surface plasmon resonance and lipid monolayer analyses. Ca 2+ ions mainly slow the membrane...
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Biochem J (2000) 352 (1): 191–196.
Published: 07 November 2000
...; CK2, casein kinase 2 ; I-2, inhibitor 2 of protein phosphatase 1; PrP, prion protein ; PrPc, cellular PrP; PrPSc, pathological isoform of PrP; SPR, surface plasmon resonance. 1 To whom correspondence should be addressed (e-mail pinna!civ.bio.unipd.it). not affect its catalytic activity. The opposite...
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Biochem J (2000) 345 (3): 627–636.
Published: 25 January 2000
... correspondence should be addressed (e-mail jungbaue@;hp01.boku.ac.at). 21 7 1999 5 10 1999 15 11 1999 The Biochemical Society, London © 2000 2000 co-operativity cyclophilin heat-shock proteins real-time biological interaction assay surface plasmon resonance Biochem. J. (2000...
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Biochem J (1999) 342 (3): 605–613.
Published: 05 September 1999
..., soluble and partially glycosylated protein were purified under non-denaturing conditions and the identities of mature-length aglycosyl recPrP and two cleavage fragments were determined by electrospray MS. Binding was assessed by surface plasmon resonance techniques using both direct and competitive ligand...
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Biochem J (1999) 340 (2): 411–416.
Published: 25 May 1999
..., Japan. 2 To whom correspondence should be addressed (e-mail antibiot@postman.riken.go.jp). 23 11 1998 22 2 1999 12 3 1999 The Biochemical Society, London © 1999 1999 microtubule network programmed cell death surface plasmon resonance tubulin Biochem. J. (1999...
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