... selection system random mutagenesis analysis retro viral protease substrate recognition The Human immunodeficiency virus (HIV) protease is responsible for the sequential processing of the viral Gag and Gag-Pol polyproteins [ 1–4 ], in which the protease itself and the other structural proteins...

...). Published by Portland Press Limited on behalf of the Biochemical Society 2021 base excision repair DNA glycosylase substrate recognition Guanine:thymine mispairs are the most abundant mismatches found in genomic DNA ( Figure 1 ) [ 1–3 ]. These mismatches are produced by various pathways...

..., phosphatidic acid and 1-inositol phosphate. Comparisons of these structures with the wild-type SaPLD show a larger binding site able to accommodate a bulkier secondary alcohol substrate as well as changes to the position of a flexible surface loop proposed to be involved in substrate recognition. The complex...

... small-molecule inhibitors substrate recognition Many aspects of LIMK1 structure and function are still to be completely characterised. A significant development was the recent publication of a 3.5 Å model of the LIMK1–CFL1 complex [ 24 ]. However, there remain open questions regarding LIMK1's...

... selectivity. Among the 13 identified sites, H2BK116su is the most favorable Sirt5 substrate, with H4K12su showing the lowest catalytic efficiency and no activity observed in the presence of H4K31su. To investigate the mechanisms associated with substrate recognition and catalysis, we solved the crystal...

... stabilization and molecular mechanism for substrate recognition of αTAT1 1 To whom correspondence should be addressed (email hsumi@med.kyushu-u.ac.jp ). Crystal structures of the αTAT domain of MEC-17 in complex with acetoacetyl-CoA at a resolution of 1.25 Å and in complex with CoA at resolutions...

... a stabilized enol intermediate. decarboxylase mechanism enzyme evolution oxaloacetate analogue oxaloacetate decarboxylase pyruvate kinase substrate recognition 13 6 2013 14 11 2013 13 12 2013 13 12 2013 1 To whom correspondence should be addressed (email...

... of β1,4-GalT7 fused with the maltose-binding protein to study substrate recognition. Calorimetric binding studies showed that the binding of the donor substrate UDP-Gal largely promoted binding of the acceptor substrate. To identify the structural basis governing substrate recognition, we used a fragment...

... in the function of MDR1. 1 To whom correspondence should be addressed (email uedak@kais.kyoto-u.ac.jp ). 27 4 2006 3 10 2006 10 10 2006 10 10 2006 The Biochemical Society, London 2007 cholesterol drug-binding pocket multidrug resistance substrate recognition...

..., London ©2003 2003 chimaera glucose transporter saturation mutagenesis substrate recognition yeast Abbreviations used: MFS, major facilitator superfamily; TM, transmembrane segment. Biochem. J. (2003) 372, 247 252 (Printed in Great Britain) 247 Transmembrane segments 1, 5, 7 and 8...

...%. These structures provide important new insights into substrate recognition and ligand-induced conformational changes. Comparison of these structures with the structures of OTCase complexed with the bisubstrate analogue PALO or CP and L -norvaline reveals that binding of the first substrate, CP, induces a global...

...Julio J. CARAMELO; Jorge FLORÍN-CHRISTENSEN; Mónica FLORÍN-CHRISTENSEN; José M. DELFINO A set of radioiodinatable phosphatidylcholines (PCs) derivatized with the Bolton-Hunter reagent (BHPCs) was synthesized to probe the substrate recognition and activity of phospholipases. A common feature...