... 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 clathrin-mediated endocytosis immunofluorescence microscopy intracellular trafficking membrane raft subcellular fractionation transforming growth factor β receptor (TGFβR) The TGFβ (transforming growth...

... higher than in most cells (2.5–4% of PtdIns4 P ). Using subcellular fractionation, we found that the majority of the basal PtdIns5 P is present in the plasma membrane, but it is also enriched in intracellular membrane compartments, especially in SER (smooth endoplasmic reticulum) and/or Golgi, where high...

... phosphatidylinositol kinase phosphoinositide signalling subcellular fractionation Abbreviations used: β-OG, β-octylglucoside; DOC, deoxycholate; ER, endoplasmic reticulum; PI, phosphatidylinositol; PI4K, phosphatidylinositol 4-kinase; PI4P, phosphatidylinositol 4-phosphate; PIPK, phosphatidylinositol 4...

... should be addressed (e-mail j.hsuan@rfc.ucl.ac.uk ). 8 1 2003 11 3 2003 20 3 2003 21 3 2003 The Biochemical Society, London ©2003 2003 endoplasmic reticulum phosphatidylinositol kinase phosphoinositide signalling subcellular fractionation valosin-containing protein...

... of Bcl-x L but not Bcl-2. apoptosis IL-3 mitochondria subcellular fractionation translocation Abbreviations used: TM, transmembrane; HM, heavy membrane; BH, Bcl-2homology; FCS, fetal calf serum; IL-3, interleukin-3. Biochem. J. (2001) 359, 345 352 (Printed in Great Britain) 345...

... antigen-DM, where peptide loading can occur. Key words: endocytosis, intracellular traffic, MHC-class II molecules, subcellular fractionation. led to presentation via both newly synthesized and recycling class II molecules [15,16]. This implies that there are different endocytic pathways with non...