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Biochem J (2015) 466 (3): 431–442.
Published: 06 March 2015
... studies suggest that Rif plays no critical functions in platelets, likely due to functional overlap with other Rho GTPases. In contrast, RhoG is essential for normal granule secretion downstream of the collagen receptor GPVI. The central defect in RhoG −/− platelets is reduced dense granule secretion...
Biochem J (2015) 466 (2): 203–218.
Published: 20 February 2015
...Guy A. Rutter; Timothy J. Pullen; David J. Hodson; Aida Martinez-Sanchez Insulin release from pancreatic β-cells is required to maintain normal glucose homoeostasis in man and many other animals. Defective insulin secretion underlies all forms of diabetes mellitus, a disease currently reaching...
Ilaria Canobbio, Gianni F. Guidetti, Barbara Oliviero, Daria Manganaro, Dina Vara, Mauro Torti, Giordano Pula
Biochem J (2014) 462 (3): 513–523.
Published: 22 August 2014
... in platelet intracellular Ca 2+ that stimulates α-granule and dense granule secretion and leads to the release of the secondary agonist ADP. Released ADP acts in an autocrine manner as a stimulant for critical signalling pathways leading to the activation of platelets. This includes the activation...
Includes: Supplementary data
Biochem J (2014) 458 (3): 491–498.
Published: 28 February 2014
...Yue Wang; Yusuf Ali; Chun-Yan Lim; Wanjin Hong; Zhiping P. Pang; Weiping Han Numerous studies have focused on the regulation of leptin signalling and the functions of leptin in energy homoeostasis; however, little is known about how leptin secretion is regulated. In the present study we studied...
Includes: Supplementary data
Kyubo Kim, Youlia M. Petrova, Brenton L. Scott, Rupesh Nigam, Anurag Agrawal, Christopher M. Evans, Zoulikha Azzegagh, Alejandra Gomez, Elsa M. Rodarte, Vesa M. Olkkonen, Rustam Bagirzadeh, Lucia Piccotti, Binhui Ren, Joo-Heon Yoon, James A. McNew, Roberto Adachi, Michael J. Tuvim, Burton F. Dickey
Biochem J (2012) 446 (3): 383–394.
Published: 28 August 2012
... Airway mucin secretion and MC (mast cell) degranulation must be tightly controlled for homoeostasis of the lungs and immune system respectively. We found the exocytic protein Munc18b to be highly expressed in mouse airway epithelial cells and MCs, and localized to the apical pole of airway secretory...
Includes: Supplementary data
Xiaodi Yu, Ganeshram R. Visweswaran, Zoe Duck, Srisailam Marupakula, Sheila MacIntyre, Stefan D. Knight, Anton V. Zavialov
Biochem J (2009) 418 (3): 541–551.
Published: 25 February 2009
... displayed significant structural similarity. Although UMD is in the middle of the predicted amphipathic β-barrel of Caf1A, the usher still assembled in the membrane in the absence of this domain. UMD did not bind Caf1M–Caf1 complexes, but its presence was shown to be essential for Caf1 fibre secretion...
Includes: Supplementary data
Biochem J (2005) 389 (3): 803–812.
Published: 26 July 2005
...Julianne T. Djordjevic; Maurizio Del Poeta; Tania C. Sorrell; Kylie M. Turner; Lesley C. Wright The secreted, multifunctional enzyme PLB1 (phospholipase B1 protein encoded by the PLB1 gene) is a virulence determinant of the pathogenic fungus Cryptococcus neoformans , but the mechanism of its...
Biochem J (2005) 389 (1): 117–125.
Published: 21 June 2005
... in the nematode's secretions. The deduced amino acid sequence of the gene is very similar to glycosyl hydrolase family 16 proteins. The recombinant protein, expressed in Escherichia coli , preferentially hydrolysed the β-1,3-glucan laminarin, and had very low levels of activity on β-1,3-1,4-glucan, lichenan...
Biochem J (2005) 386 (3): 489–495.
Published: 08 March 2005
...Houssain BENABDELHAK; Lutz SCHMITT; Carsten HORN; Kornelia JUMEL; Mark A. BLIGHT; I. Barry HOLLAND The ATPase activity of the ABC (ATP-binding cassette) ATPase domain of the HlyB (haemolysin B) transporter is required for secretion of Escherichia coli haemolysin via the type I pathway. Although ABC...
Biochem J (2005) 385 (2): 347–353.
Published: 07 January 2005
... complexes that probably regulate specific steps in the fusion cycle. We had previously shown that overexpression of Sept5 blocked secretion [ 7 ], suggesting an effect on the interaction of syntaxin with components of the core fusion machinery. Structural analyses involving syntaxin 1A fragments have...
Biochem J (2004) 380 (2): 441–448.
Published: 01 June 2004
...Alexandre MARÉCHAL; Pierre-Luc TANGUAY; Mario CALLEJO; Renée GUÉRIN; Guy BOILEAU; Luis A. ROKEACH Folding of newly synthesized proteins within the ER (endoplasmic reticulum) is a rate-limiting step in protein secretion. Thus ER molecular chaperones and foldases have a major impact in determining...
Biochem J (2004) 377 (1): 121–130.
Published: 01 January 2004
... in a functional truncated apoB variant (apoB-29) and abolished by mutagenesis. This Cys-1085Ser mutation resulted in secretion of smaller and denser lipoprotein particles containing 80% less cholesteryl ester and triacylglycerol than wild-type controls. We show that palmitoylation of apoB-29 occurs in the ER...
Biochem J (2003) 374 (1): 207–217.
Published: 15 August 2003
... proteins, we examined the effects of Munc18-based peptides (Munc18a peptide 3 and Munc18c peptide 3). Addition of the peptides to permeabilized platelets inhibits secretion from all three platelet granules. These peptides also inhibit agonist-induced aggregation in saponin-permeabilized platelets...
Gabriela da SILVA XAVIER, Isabelle LECLERC, Aniko VARADI, Takashi TSUBOI, S. Kelly MOULE, Guy A. RUTTER
Biochem J (2003) 371 (3): 761–774.
Published: 01 May 2003
... achieved pharmacologically with 5-amino-4-imidazolecarboxamide riboside (AICAR), or by adenoviral overexpression of a truncated, constitutively active form of the enzyme (AMPKα1.T 172 D), blocked glucose-stimulated insulin secretion. In MIN6 cells, activation of AMPK suppressed glucose metabolism...
Biochem J (2003) 370 (2): 641–649.
Published: 01 March 2003
... methods developed in this study. 1 To whom correspondence should be addressed (e-mail firstname.lastname@example.org ). 6 11 2002 15 11 2002 15 11 2002 The Biochemical Society, London ©2003 2003 mathematical analysis membrane fusion octadecylrhodamine B-chloride (R18) secretion...
Biochem J (2002) 367 (3): 791–799.
Published: 01 November 2002
... 23 7 2002 The Biochemical Society, London ©2002 2002 PI 3-kinase promoter secretion transcription Abbreviations used: CRE, cAMP response element; EBV, Epstein—Barr virus; IκB, inhibitor κB; IKK, IκB kinase; jfc1-LUC, jfc1 luciferase construct; NF-κB, nuclear factor-κB; PI...
Biochem J (2002) 363 (3): 737–744.
Published: 24 April 2002
... transport secretion ubiquitination endocytosis Abbreviations used: CMAC-Arg, 7-amino-4-chloromethylcoumaryl- l -arginine amide dihydrochloride; GFP, green fluorescent protein; ORF, open reading frame; Ub, ubiquitin. Biochem. J. (2002) 363, 737 744 (Printed in Great Britain) 737 Utilization...
Biochem J (2001) 360 (3): 645–649.
Published: 10 December 2001
...Renu K. JAIN; Paul B. M. JOYCE; Miguel MOLINETE; Philippe A. HALBAN; Sven-Ulrik GORR Green fluorescent protein (GFP) is used extensively as a reporter protein to monitor cellular processes, including intracellular protein trafficking and secretion. In general, this approach depends on GFP acting...
Alois HODEL, Seong J. AN, Neal J. HANSEN, Jared LAWRENCE, Barbara WÄSLE, Michael SCHRADER, J. Michael EDWARDSON
Biochem J (2001) 356 (3): 843–850.
Published: 08 June 2001
... between granules . There is now considerable interest in identifying other proteins that contribute to the secretion of zymogens from the acinar cell. Syncollin is a zymogen granule protein that was originally isolated through its ability to bind to syntaxin in a Casensitive manner . This property...
Biochem J (2000) 350 (2): 463–468.
Published: 23 August 2000
... 2000 27 6 2000 The Biochemical Society, London © 2000 2000 milk protein phosphorylation secretion secretory pathway Biochem. J. (2000) 350, 463 468 (Printed in Great Britain) 463 Purification of Golgi casein kinase from bovine milk Jennifer S. DUNCAN*1, Mark C. WILKINSON...
Biochem J (2000) 349 (1): 247–253.
Published: 26 June 2000
... lung protein profile secretion Biochem. J. (2000) 349, 247 253 (Printed in Great Britain) 247 Definition of Munc13-homology-domains and characterization of a novel ubiquitously expressed Munc13 isoform Henriette KOCH*, Kay HOFMANN and Nils BROSE*1 *Max-Planck-Institut fu$ r experimentelle Medizin...
Biochem J (2000) 347 (3): 601–612.
Published: 25 April 2000
... interactions, secretion, SecY}Sec61, targeting signal. peripheral ATPase SecA . The SecYEG complex forms the actual channel through which precursors are guided to the periplasm and provides the high-affinity binding site for SecA at the membrane . Proteins destined for translocation are synthesized...
Yoshihiko URATANI, Keiko TAKIGUCHI-HAYASHI, Nobuhiko MIYASAKA, Michio SATO, Ming-hao JIN, Yasuyoshi ARIMATSU
Biochem J (2000) 346 (3): 817–826.
Published: 07 March 2000
... secretion Biochem. J. (2000) 346, 817 826 (Printed in Great Britain) 817 Latexin, a carboxypeptidase A inhibitor, is expressed in rat peritoneal mast cells and is associated with granular structures distinct from secretory granules and lysosomes Yoshihiko URATANI1, Keiko TAKIGUCHI-HAYASHI, Nobuhiko...
Biochem J (1999) 343 (3): 505–515.
Published: 25 October 1999
..., diacylglycerol, fatty acids, liver, secretion. notably citrate (activation) and long-chain acyl-CoA esters (inhibition). Reversible phosphorylation at several serine residues plays a major role in the regulation of ACC activity [2,5]. The kinases that are thought to be most important in io are cAMP- dependent...
Charlotte V. BURKE, Carol W. BUETTGER, Elizabeth A. DAVIS, Steven J. MCCLANE, Franz M. MATSCHINSKY, Steven E. RAPER
Biochem J (1999) 342 (2): 345–352.
Published: 24 August 1999
... the human wild-type islet GK or one of four mutant forms of GK, (the MODY-2 mutants E70K, E300K and V203A and the GK-HI mutant V455M) were transduced into glucose-responsive insulin-secreting β-HC9 cells and tested functionally in order to initiate the first analysis in vivo of recombinant wild-type...
Biochem J (1999) 341 (3): 563–569.
Published: 26 July 1999
..., including glucose and depolarizing concentrations of K + . In MIN6 cells, the glucose dose-response and time course of phogrin phosphorylation paralleled that of insulin secretion. Like secretion, glucose- or K + -stimulated phosphorylation required the presence of Ca 2+ . The calmodulin antagonist W-7...
Biochem J (1999) 341 (2): 453–460.
Published: 08 July 1999
... from the wild-type cells. The excess loss of labelled TAG was primarily due to an increased TAG fatty acid oxidation. Free fatty acid release into the medium was not increased in the transfectants, nor was the very low rate of lipoprotein lipid secretion. Also, there was no increased net trafficking...
Biochem J (1999) 339 (3): 639–647.
Published: 26 April 1999
... with either a secreted form of the human insulin pro-receptor or the precursor form of von Willebrand factor resulted in both proproteins being processed; RPE.40 cells were unable to process either precursor protein in the absence of co-expressed PACE4. Northern analysis demonstrated that untransfected RPE.40...
Biochem J (1999) 339 (2): 299–307.
Published: 08 April 1999
... secretion yeast Biochem. J. (1999) 339, 299 307 (Printed in Great Britain) 299 Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein Arthur L. KRUCKEBERG, Ling YE, Jan A. BERDEN and Karel VAN...
Biochem J (1999) 337 (3): 471–481.
Published: 25 January 1999
..., the interaction with the growth factor alters the distribution of heparan sulphate-degradation products, presumably by preventing secretion of the short heparanase-derived species. Our findings show that most of the free and bFGF-bound heparan sulphate chains are destined for lysosomes, which would be consistent...