... 8 6 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 mucin multigene family quantitative PCR TcSMUG Trypanosoma cruzi The protozoan Trypanosoma cruzi is the etiological agent of Chagas' disease. In spite of recent successes in disrupting vector...

... lM) and by rapamycin (50 nM), suggesting that the PI 3- kinase}protein kinase B}p70 S6 kinase pathway could be in- volved in the stimulation of grb-1 gene expression by insulin in human muscle cells. Key words: grb-1, insulin signalling, myotubes, quantitative PCR. molecules [1,3]. Among...

... hybridization, which can be variable. We have directly measured gene and mRNA copy number by using real-time quantitative PCRs in mosquitoes. The acquisition of fluorescence from incorporation of the double-strand-specific dye SYBR GreenI into a PCR product once per cycle is used to provide an absolute...