... anthony.michael@utsouthwestern.edu . 3 3 2016 20 4 2016 22 4 2016 © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2016 biosynthetic diversity convergent evolution polyamine putrescine spermidine spermine Another prominent...

...Radika Soysa; Hanka Venselaar; Jacqueline Poston; Buddy Ullman; Marie-Pierre Hasne The TcPOT1.1 gene from Trypanosoma cruzi encodes a high affinity putrescine-cadaverine transporter belonging to the APC (amino acid/polyamine/organocation) transporter superfamily. No experimental three-dimensional...

... harvesting. Polyamines were depleted by incubation in 5 mM DFMO. We have previously reported that maximal polyamine depletion occurs after 4 days of treatment with 5 mM DFMO [ 4 ]. Within 6 h of DFMO treatment putrescine was undetectable, spermidine was absent after 24 h, and 40% of spermine remained after 4...

...-regulated genes linked to either carcinogenesis or apoptosis. 1 To whom correspondence should be addressed (e-mail h.m.wallace@abdn.ac.uk ). 1 9 2003 16 9 2003 18 9 2003 18 9 2003 The Biochemical Society, London ©2003 2003 apoptosis cancer cell growth putrescine...

...John L.A. MITCHELL; Aviva LEYSER; Michelle S. HOLTORFF; Jill S. BATES; Benjamin FRYDMAN; Aldonia L. VALASINAS; Venodhar K. REDDY; Laurence J. MARTON The polyamines spermidine and spermine and their diamine precursor putrescine are essential for mammalian cell growth and viability, and strategies...

...Yong-Sun LEE; Young-Dong CHO The cDNA encoding ornithine decarboxylase (ODC; EC 4.1.1.17), a key enzyme in putrescine and polyamine biosynthesis, has been cloned from Nicotiana glutinosa (GenBank® AF 323910), and was expressed in Escherichia coli . The amino acid sequence of N. glutinosa ODC showed...

... the control of the mouse metallothionein (MT) I promoter. In comparison with singly transgenic animals overexpressing SSAT, the doubly transgenic mice unexpectedly displayed much more striking signs of activated polyamine catabolism, as exemplified by a massive putrescine accumulation and an extreme reduction...

..., an active-transport system allowing the cell to take up polyamines from its environment becomes critical for normal growth [4,5]. In mammalian cells, the major polyamines are spermidine and spermine, with smaller amounts of their precursor putrescine. Spermine is formed by the action...

... the high levels of antizyme, endogenous ODC activity was not completely abolished, with 10– 39% remaining, depending on the transgenic line. Additionally, a relatively small decrease (30–32%) in cardiac spermidine content was observed, with levels of putrescine and spermine unaffected. Interestingly...

...Kazuei IGARASHI; Keiko KASHIWAGI The polyamine content of cells is regulated by biosynthesis, degradation and transport. In Escherichia coli , the genes for three different polyamine transport systems have been cloned and characterized. Two uptake systems (putrescine-specific and spermidine...

... transgenic animals. Liver was the most affected tissue in the MT–SSAT transgenic animals, revealed by putrescine overaccumulation, significant decrease in spermidine concentration and > 90% reduction in the spermine pool. Even though hepatic SSAT mRNA accumulated to massive levels in non-induced...