... by EPAC1 activation provides novel insights into late CFTR trafficking, insertion and/or stabilization at the PM and highlighs new potential therapeutic targets to tackle CF disease. actin cytoskeleton cAMP CFTR EPAC1 protein trafficking Cystic Fibrosis (CF) is the most common lethal...

... secretion N-linked glycosylation protein stabilization protein trafficking For immunocytochemistry, cells were fixed in 4% paraformaldehyde at room temperature for 15 min, permeabilized in 0.25% Triton X-100 for 5 min, and then stained using ERp72 or ERGIC-53 primary antibodies. The secondary...

...) exchanger 1 (kAE1) can cause genetic distal renal tubular acidosis (dRTA). Different SLC4A1 mutations give rise to mutant kAE1 proteins with distinct defects in protein trafficking. The mutant kAE1 protein may be retained in endoplasmic reticulum (ER) or Golgi apparatus, or mis-targeted to the apical...

.... As for many other membrane proteins, its intracellular sorting and distribution have a tremendous effect on its function, and constitute an important aspect of its regulation. Many short sequences have been shown to contribute to protein trafficking along the sorting or endocytic pathways. Using...

... protein trafficking The PLG/PLA (plasminogen/plasmin) system has been linked to a variety of pericellular proteolytic processes in embryonic development [ 1 ], monocyte/macrophage recruitment [ 2 , 3 ], wound healing [ 4 , 5 ], angiogenesis [ 4 , 6 ] and tumour cell invasion [ 7 – 9...

... and participates during rescue of a common disease-associated mutation. Therapeutic strategies to overcome clinically important defects in cystic fibrosis should consider the role of palmitoylation as a molecular target. cystic fibrosis palmitoylation post-translational modification protein trafficking...

...–phenotype relationship human ethera-go-go -related gene KCNH2 long-QT syndrome protein trafficking Loss-of-function mutations in membrane-spanning proteins usually result in protein misfolding, which leads to enhanced degradation and hence decreased levels of functional protein at the plasma...

... effects were observed in cells enriched with lipoprotein-derived cholesterol and in primary human macrophages. Removal of excess cholesterol by a cyclodextrin vehicle restored apoE secretion, indicating that the transport defect was reversible. That cholesterol impaired protein trafficking was supported...

... use, distribution and reproduction in any medium, provided the original work is properly cited. doppel (Dpl) epithelial cell lipid raft prion protein (PrP) protein trafficking The PrP C (cellular prion protein) is a cell-surface glycoprotein of unknown function expressed in mammalian...

... drugs (e.g. [ 5 ]). cystic fibrosis transmembrane conductance regulator (CFTR) ΔF508 rescue protein trafficking streptavidin pulldown Cell-surface expression can be assessed by chemical biotinylation reagents that consist of biotin attached to a reactive sulfonated NHS ( N...

... to this work. digestive vacuole green fluorescent protein (GFP) pH plasmepsin Plasmodium falciparum protein trafficking 2 To whom correspondence should be addressed (email l.tilley@latrobe.edu.au ). 13 7 2007 9 8 2007 14 8 2007 14 8 2007 © The Authors Journal...

... developmentally down-regulated gene 4-2 (Nedd4-2) protein trafficking ubiquitin COS7 cells were transfected with HA–ENaC subunits in 35 mm dishes, and six plates were used for one experiment. Medium was replaced 6–8 h after transfection with fresh medium containing 10 μM amiloride to block the channel...

...) prenylated Rab acceptor 1 (PRA1) prenylin protein trafficking Yip3 Abbreviations used: BHK, baby hamster kidney; BMH, bismaleimidohexane; DSS, disuccinyl suberate; ER, endoplasmic reticulum; GFP, green fluorescent protein; Man II, mannosidase II; WT, wild-type; D176A etc., Asp 176 →Ala...

... LSB6 phosphoinositide protein trafficking yeast Abbreviations used: GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GFP, green fluorescent protein; HA, haemagglutinin; LSB6 , L a s 17p- b inding protein; ORF, open reading frame; PI 4-kinase, phosphoinositide 4-kinase; PI4KII, PI 4...

... assembly membrane integration protein trafficking Abbreviations used: Cx, connexin; DTT, dithiothreitol; TEA, triethanolamine. Biochem. J. (2002) 365, 693 699 (Printed in Great Britain) 693 Post-translational integration and oligomerization of connexin 26 in plasma membranes and evidence...

... correspondence should be addressed (e-mail josh.hamilton@dartmouth.edu ). 27 10 2000 8 1 2001 14 2 2001 The Biochemical Society, London © 2001 2001 ATP-binding cassette protein multidrug resistance protein trafficking Biochem. J. (2001) 355, 617 624 (Printed in Great Britain...

... (e-mail stefan.broeer@anu.edu.au ). 15 11 2000 24 1 2001 19 2 2001 The Biochemical Society, London © 2001 2001 amino acid transport heterodimeric transporter protein trafficking Biochem. J. (2001) 355, 725 731 (Printed in Great Britain) 725 Association of 4F2hc...

... to GenBank under the accession numbers AF230802 and AF230803 respectively. calcium protein trafficking receptor-stimulated channel store-operated channel vomeronasal organ Biochem. J. (2000) 351, 115 122 (Printed in Great Britain) 115 Cloning, expression and subcellular localization of two novel...

... internalization protein trafficking zinc uptake Biochem. J. (2000) 346, 329 336 (Printed in Great Britain) 329 Zinc-regulated ubiquitin conjugation signals endocytosis of the yeast ZRT1 zinc transporter Raad S. GITAN and David J. EIDE1 Department of Nutritional Sciences, 217 Gwynn Hall, University...