... of the organelle. The protein import into mitochondria is mediated by diverse import pathways and is continuously under watch by quality control systems. However, it is often challenged by both internal and external factors, such as oxidative stress or energy shortage. The impaired protein import and biogenesis...

... characterised in great detail in yeast over the last several decades, yet many of the mechanisms that regulate these processes in higher eukaryotes are still unknown. In this review, we highlight the current knowledge of mitochondrial protein import in plants. Despite the fact that the mechanisms...

...Lisa MacPherson; Kostas Tokatlidis Mitochondria fulfill a diverse range of functions in cells including oxygen metabolism, homeostasis of inorganic ions and execution of apoptosis. Biogenesis of mitochondria relies on protein import pathways that are ensured by dedicated multiprotein translocase...

...-terminal residues (Arg3, Lys7 and Arg13) by Ala abolished import. We conclude that the synergistic interaction of helices α1 and α2 is crucial for the highly efficient import of hGDHs into mitochondria. glutamate dehydrogenase human mitochondria presequence protein import To create...

... mitochondria NADH dehydrogenase protein import respiratory chain subcomplex Proton-pumping NADH:ubiquinone oxidoreductase (complex I, EC 1.6.5.3) is the largest membrane protein complex in the respiratory chain of many bacteria and mitochondria [ 1 – 3 ]. Coupling the two-electron transfer reaction...

...Henrik Aronsson; Paul Jarvis Pre-protein import into chloroplasts is facilitated by multiprotein translocon complexes in the envelope membranes. Major components of the TOC (translocon at the outer envelope membrane of chloroplasts) complex are the receptor proteins Toc33 and Toc159. These two...

... The Authors Journal compilation © 2011 Biochemical Society 2011 aminoacyl-tRNA synthetase organelle pathology-related mutation protein import translation machinery translocation The mitochondrion has many fundamental functions, e.g. in metabolic pathways, redox processes, energy production...

... membrane proteins [e.g. VSG (variant surface glycoprotein)] is crucial for the establishment and maintenance of an infection by T. brucei . Transport of a majority of proteins to the plasma membrane involves their translocation into the ER (endoplasmic reticulum). Thus inhibition of protein import...

... by these specialized pathways with changes in gene expression is critical for plastid and plant development. Moreover, protein import is acutely regulated in response to physiological and metabolic changes within the cell. In the present review we summarize the current knowledge of the mechanism of import via...

... of Pex5p in cells lacking components of the peroxisomal AAA (ATPases associated with various cellular activities) or Pex4p–Pex22p complexes of the peroxisomal protein import machinery and in cells affected in proteasomal degradation. In cells lacking components of the Pex4p–Pex22p complex, mono...

..., London 2004 mitochondria protein import Saccharomyces cerevisiae transcription factor The mitochondrial (mt) genome encodes a few mt proteins, but most of the mt proteins (approx. 95%) including the mt transcription/translational machinery are nuclear gene products, which...

... was immuno-detected. However, pSS fragments with molecular masses between those of iSS and SS were present even in the ribulose-1,5-bisphosphate carboxylase}oxygenase holoenzyme. Key words: chloroplast, protein import, stromal processing peptidase, transformation. respectively, of pSS from Chlamydomonas...

... of peroxisomes and in the cytosol. 1 To whom correspondence should be addressed (e-mail paul.vanveldhoven@med.kuleuven.ac.be ). 5 10 2001 25 3 2002 3 4 2002 The Biochemical Society, London ©2002 2002 biogenesis peroxin protein import thiolase WD40 Abbreviations...

..., human PEX5Rp did not bind to Pex14p or to the RING finger motif of Pex12p, and could not restore PTS1 protein import in Pex5 −/− mouse fibroblasts. Immunofluorescence analysis of epitope-tagged PEX5Rp in Chinese hamster ovary cells suggested an exclusively cytosolic localization. Northern-blot analysis...

... correspondence should be addressed (e-mail e-glaser@biokemi.su.se ). 27 3 2000 7 6 2000 13 6 2000 The Biochemical Society, London © 2000 2000 protein import proteolysis signal peptide Biochem. J. (2000) 349, 703 707 (Printed in Great Britain) 703 RESEARCH COMMUNICATION Rapid...

.... These data demonstrate that targeting of C-terminally anchored proteins to mitochondria is sequence specific and mediated by a saturable mechanism. Neither ATP nor chaperone proteins are strictly required for either specific targeting or membrane insertion. Key words: import pathway, protein import, rat...

... Chlamydomonas reinhardtii isolated chloroplasts protein import small subunit ribulose-1,5-bisphosphate carboxylase two-step processing Biochem. J. (1999) 344, 391 395 (Printed in Great Britain) 391 A processing intermediate of a stromal chloroplast import protein in Chlamydomonas Qingxiang SU1, Philipp...

... fragment of the F 1 β precursor that contains all information necessary for mitochondrial targeting and processing and that can be used for structural and functional studies of the mitochondrial protein import system. This procedure has a general value because it can be used for the production of chemical...