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Keywords: protein engineering
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Biochem J (2023) 480 (2): 127–140.
Published: 23 January 2023
... 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 protein engineering signaling pathways synthetic biology More than six decades ago, Jacob and Monod proposed a groundbreaking perspective on how genetic information is organized...
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Biochem J (2021) 478 (15): 3047–3062.
Published: 13 August 2021
... enzyme activation metalloenzymes molecular mechanisms protein engineering Metals are present as structural features in over one-third of proteins, underscoring their importance for protein structure and function [ 1 , 2 ]. The incorporation of divalent metal ions into protein structures...
Includes: Supplementary data
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Biochem J (2020) 477 (9): 1701–1719.
Published: 11 May 2020
... ∑ ⁡ # read s mut , i where # reads mut, i is the number of reads of a mutant in library fraction i , and ∑ # reads mut, i is the sum of all the reads for all mutants in library fraction i . metalloproteases next-generation sequencing protease inhibitor protein engineering...
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Biochem J (2020) 477 (8): 1483–1497.
Published: 29 April 2020
... to previous studies from a structural perspective. These clashes would potentially lead to reduced substrate binding affinity of McTadA, consistent with our in vitro deamination activity and binding assays. To rescue the deamination activity of McTadA, we carried out two rounds of protein engineering through...
Includes: Supplementary data
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Biochem J (2019) 476 (1): 151–164.
Published: 15 January 2019
... of neutral lipids (Δ F TAG) as a function of OD 600 (Δ F TAG/OD 600 ). Arabidopsis thaliana error-prone PCR LACS protein engineering Saccharomyces cerevisiae Fatty acids are carboxylic acids with highly reduced acyl chains, which act as the major energy reservoir in eukaryotic...
Includes: Supplementary data
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Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
... of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our dependence on fossil resources and a shift in focus...
Includes: Supplementary data
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Biochem J (2018) 475 (7): 1335–1352.
Published: 16 April 2018
... evolution protease inhibitor protein engineering protein–protein interactions (PPIs) serine proteases Here, we constructed a combinatorial library of APPI mutants that was rich in mutations enhancing stability, affinity, specificity and rapid association. By using this YSD APPI library to conduct...
Includes: Supplementary data
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Biochem J (2016) 473 (20): 3611–3620.
Published: 11 October 2016
... 2016 15 8 2016 15 8 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 protein engineering protein stability resurrected proteins thioredoxin The engineering of proteins with enhanced stability has drawn considerable...
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Biochem J (2016) 473 (11): 1563–1578.
Published: 27 May 2016
... candidates compared with current biopharmaceutical treatments. Aβ peptide lipocalin neurodegeneration protein engineering Alzheimer's disease (AD) is the most prevalent form of dementia, with 10% of the human population older than 65 years and 40% older than 85 years affected [ 1 ]. Apart...
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Biochem J (2011) 435 (2): 345–354.
Published: 29 March 2011
... ). 1 9 2010 14 1 2011 4 2 2011 4 2 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 haloalkane dehalogenase phylogenetic analysis principal component analysis protein engineering substrate specificity Enzymes are biological catalysts...
Includes: Supplementary data
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Biochem J (2011) 435 (1): 1–16.
Published: 15 March 2011
... © 2011 Biochemical Society 2011 procoagulant protease therapy protein degradation protein engineering trypsin fold Sequencing of the human genome revealed that more than 2% of our genes encode proteases, suggesting that these enzymes possess functions more complex than the simple...
Includes: Supplementary data
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Biochem J (2011) 435 (1): 55–63.
Published: 15 March 2011
...://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. avidin biotin protein engineering protein–ligand interaction streptavidin traptavidin The capture of the small molecule...
Includes: Supplementary data
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Biochem J (2008) 414 (2): 205–214.
Published: 12 August 2008
... 2008 chaperonin CO 2 fixation directed evolution protein engineering RbcX ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco [ribulose-P 2 (D-ribulose-1,5-bisphosphate) carboxylase/oxygenase; EC 4.1.1.39] is the enzyme employed by almost all photoautotrophic organisms...
Includes: Supplementary data
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Biochem J (2007) 404 (3): 517–524.
Published: 29 May 2007
...) or processed for SDS/PAGE (12% gels) and non-denaturing PAGE (4–12% acrylamide gradient) analysis as described previously [ 17 ]. CO 2 fixation directed evolution metabolic engineering protein engineering ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco (ribulose-1,5...
Includes: Supplementary data
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Biochem J (2006) 397 (2): 305–312.
Published: 28 June 2006
.... bioluminescence assay firefly luciferase imaging Photinus pyralis protein engineering Beetle luciferases catalyse the efficient transfer of chemical energy into light via a two-step process, utilizing ATP-Mg 2+ , firefly luciferin ( D -LH 2 ) and molecular oxygen, yielding oxyluciferin (LO...
Includes: Supplementary data
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Biochem J (2006) 394 (1): 85–93.
Published: 27 January 2006
... The Biochemical Society, London 2006 cyclic protein cyclotide haemolytic activity kalata NMR protein engineering The cyclotides [ 1 ] are a fascinating family of plant proteins that are distinguished by a head-to-tail peptide backbone and six conserved cysteine residues that are paired to form...
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Biochem J (2005) 392 (3): 485–491.
Published: 06 December 2005
... chain. In contrast with wild-type avidin, which contains four identical avidin monomers, scAvd enables each one of the four avidin domains to be independently modified by protein engineering. Therefore the scAvd scaffold can be used to construct spatially and stoichiometrically defined pseudotetrameric...
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Biochem J (2004) 382 (3): 885–893.
Published: 07 September 2004
... To whom correspondence should be addressed (email Lambrou@aua.gr ). The Biochemical Society, London 2004 24 2 2004 25 5 2004 11 6 2004 15 6 2004 Cibacron Blue 3GA glutathione S-transferase herbicide detoxification ligandin protein engineering triazine dye...
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Biochem J (2001) 359 (3): 715–720.
Published: 25 October 2001
... from those of abolishing hydrogen-bond donor potential. This suggests that Trp-59 has both a local and a global stability effect by solvating a buried charge and by having a key role in the packing of the cytochrome c hydrophobic core. Key words: non-coded amino acids, protein engineering, semi...
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Biochem J (2001) 358 (1): 101–110.
Published: 08 August 2001
...2001 2001 essential dynamics herbicide detoxification molecular dynamics protein engineering Abbreviations used: CDNB, 1-chloro-2,4-dinitrobenzene; G-site, GSH binding site; GST, glutathione S-transferase; H-site, electrophile binding site; Ni-NTA, Ni 2+ -nitrilotriacetate. Biochem...
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Biochem J (2001) 354 (2): 455–463.
Published: 22 February 2001
... properties of His- 311 are important for enzyme function. An important structural role has also been attributed to cis-Pro-288. This residue may provide the key residues Gln-287 and His-311 with the proper orientation for productive binding of formate. Key words: kinetic mechanism, protein engineering, site...
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Biochem J (2000) 352 (2): 257–266.
Published: 24 November 2000
... and R184 located close to the adenosine ribose 2«-phosphate group, and R144 likely to interact with the nicotinamide ribose 5«-phosphate group. Key words: coenzyme affinity, enzyme kinetics, flavodoxin NADP+ oxidoreductase, protein engineering. with other members of the ferredoxin reductase family...
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Biochem J (2000) 345 (2): 247–254.
Published: 10 January 2000
... of the location of the ligand on the toxin and they were found to release the restrictocin fragment efficiently on proteolysis in Šitro. Key words: chimaeric toxins, protease, protein engineering, targeting, toxins. an intervening specific protease recognition site made them cytotoxic [12,13]. Chimaeric toxins...
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Biochem J (1999) 341 (1): 139–145.
Published: 24 June 1999
... activity, protein engineering, Vipera ammodytes ammodytes. have been used, from comparison of their primary and tertiary structures, chemical modification and the use of specific site- directed antibodies to acceptor-binding studies. Despite nu- merous attempts, toxic PLA # s still remain a complex multi...
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Biochem J (1999) 339 (2): 309–317.
Published: 08 April 1999
... to denaturants such as guanidine HCl and urea was revealed; the wild-type protein always proved to be the most resistant. The results obtained show the importance of hydrogen bonds and ion pairs in determining protein stability and confirm that simulation methods are able to direct protein engineering in site...