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Keywords: protein engineering
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Biochem J (2023) 480 (16): 1317–1330.
Published: 25 August 2023
...-targeting chimeras DUBs deubiquitinases GFP green fluorescent protein JAMM JAB1/MPN/Mov34 metalloproteases OTUD1 O varian tu mor domain-containing d eubiquitinase 1 UBDs ubiquitin binding domains deubiquitinases inhibitor phage display protein engineering...
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Biochem J (2023) 480 (14): 1097–1107.
Published: 17 July 2023
... ]. J.S.M. is supported by the US-Israel Binational Science Foundation (BSF) 2017207, NIH R01CA258274, Israel Cancer Research Foundation (ICRF), Israel Science Foundation (3486/20), and the U. of Toronto/HUJI research alliance in protein engineering. Open access for this article was enabled...
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Biochem J (2023) 480 (2): 127–140.
Published: 23 January 2023
... 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 protein engineering signaling pathways synthetic biology More than six decades ago, Jacob and Monod proposed a groundbreaking perspective on how genetic information is organized...
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Biochem J (2021) 478 (15): 3047–3062.
Published: 13 August 2021
... enzyme activation metalloenzymes molecular mechanisms protein engineering Metals are present as structural features in over one-third of proteins, underscoring their importance for protein structure and function [ 1 , 2 ]. The incorporation of divalent metal ions into protein structures...
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Biochem J (2020) 477 (9): 1701–1719.
Published: 11 May 2020
...@bgu.ac.il ) 3 3 2020 11 4 2020 14 4 2020 15 4 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 metalloproteases next-generation sequencing protease inhibitor protein engineering protein–protein interactions...
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Biochem J (2020) 477 (8): 1483–1497.
Published: 29 April 2020
... to previous studies from a structural perspective. These clashes would potentially lead to reduced substrate binding affinity of McTadA, consistent with our in vitro deamination activity and binding assays. To rescue the deamination activity of McTadA, we carried out two rounds of protein engineering through...
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Biochem J (2019) 476 (23): 3631–3647.
Published: 10 December 2019
... to be functional. It follows that the most ancient proteins could plausibly fold with little or no assistance. Therefore, unassisted folding may have been relevant at a primordial stage, prior to (or concomitantly with) the emergence of folding assistance. molecular evolution protein engineering protein...
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Biochem J (2019) 476 (1): 151–164.
Published: 15 January 2019
.... Weselake ( randall.weselake@ualberta.ca ) 1 10 2018 11 12 2018 17 12 2018 17 12 2018 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 Arabidopsis thaliana error-prone PCR LACS protein engineering Saccharomyces...
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Biochem J (2018) 475 (23): 3887–3901.
Published: 12 December 2018
...). Published by Portland Press Limited on behalf of the Biochemical Society 2018 homoserine malate dehydrogenase protein engineering site-directed mutagenesis synthetic biology A central challenge in the creation of a resource-efficient and sustainable bioeconomy lies in the reduction in our...
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Biochem J (2018) 475 (7): 1335–1352.
Published: 16 April 2018
... of closely related molecules, some of which perform key biological functions or serve protective roles in the context of cancer [ 8 ]. directed evolution protease inhibitor protein engineering protein–protein interactions (PPIs) serine proteases Progress curves for human trypsins were generated...
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Biochem J (2016) 473 (20): 3611–3620.
Published: 11 October 2016
... , 21071 – 21076 doi: 10.1073/pnas.1314781111 protein engineering protein stability resurrected proteins thioredoxin The engineering of proteins with enhanced stability has drawn considerable attention for many years [ 1 , 2 ]. Stabilized proteins are useful in biotechnological...
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Biochem J (2016) 473 (11): 1563–1578.
Published: 27 May 2016
... inhibit their aggregation, thus providing novel reagents to study the molecular pathology of Alzheimer's disease (AD) and alternative drug candidates compared with current biopharmaceutical treatments. Aβ peptide lipocalin neurodegeneration protein engineering Alzheimer's disease (AD...
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Biochem J (2011) 435 (2): 345–354.
Published: 29 March 2011
... ). 1 9 2010 14 1 2011 4 2 2011 4 2 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 haloalkane dehalogenase phylogenetic analysis principal component analysis protein engineering substrate specificity Enzymes are biological catalysts...
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Biochem J (2011) 435 (1): 55–63.
Published: 15 March 2011
... the program SFCHECK from CCP4. The off-rate of biotin-4-fluorescein was measured from the fluorescence increase on unbinding at 37 °C with competing free biotin, as previously described [ 26 , 31 ]. avidin biotin protein engineering protein–ligand interaction streptavidin traptavidin...
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Biochem J (2011) 435 (1): 1–16.
Published: 15 March 2011
... degradation protein engineering trypsin fold Table 1 FDA-approved protease drugs BHK, baby hamster kidney. Usage Protease Indications Source of protein Target protein or pathway Type of protease Year approved by FDA Thrombolysis Urokinase (u-PA) Thrombus, catheter...
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Biochem J (2008) 414 (2): 205–214.
Published: 12 August 2008
... /O 2 /CO 2 mixtures controlled using Aalborg GFC mass flow controllers. chaperonin CO 2 fixation directed evolution protein engineering RbcX ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco [ribulose-P 2 (D-ribulose-1,5-bisphosphate) carboxylase/oxygenase; EC...
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Biochem J (2007) 404 (3): 517–524.
Published: 29 May 2007
... PCC6301 rbc L increased the fitness of the host E. coli cell during selection. CO 2 fixation directed evolution metabolic engineering protein engineering ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyses...
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Biochem J (2006) 397 (2): 305–312.
Published: 28 June 2006
... mutations are required to generate a mutant with the desired properties. bioluminescence assay firefly luciferase imaging Photinus pyralis protein engineering Beetle luciferases catalyse the efficient transfer of chemical energy into light via a two-step process, utilizing ATP-Mg 2...
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Biochem J (2006) 394 (1): 85–93.
Published: 27 January 2006
... activity of these peptides [ 7 ]. 1 To whom correspondence should be addressed (email d.craik@imb.uq.edu.au ). 19 10 2005 18 11 2005 21 11 2005 21 11 2005 The Biochemical Society, London 2006 cyclic protein cyclotide haemolytic activity kalata NMR protein...
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Biochem J (2005) 392 (3): 485–491.
Published: 06 December 2005
... chain. In contrast with wild-type avidin, which contains four identical avidin monomers, scAvd enables each one of the four avidin domains to be independently modified by protein engineering. Therefore the scAvd scaffold can be used to construct spatially and stoichiometrically defined pseudotetrameric...
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Biochem J (2004) 382 (3): 885–893.
Published: 07 September 2004
... be addressed (email Lambrou@aua.gr ). 24 2 2004 25 5 2004 11 6 2004 15 6 2004 Cibacron Blue 3GA glutathione S-transferase herbicide detoxification ligandin protein engineering triazine dye GSTs (glutathione S-transferases; EC 2.5.1.18) are dimeric detoxification...
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Biochem J (2001) 359 (3): 715–720.
Published: 25 October 2001
... from those of abolishing hydrogen-bond donor potential. This suggests that Trp-59 has both a local and a global stability effect by solvating a buried charge and by having a key role in the packing of the cytochrome c hydrophobic core. Key words: non-coded amino acids, protein engineering, semi...
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Biochem J (2001) 358 (1): 101–110.
Published: 08 August 2001
...2001 2001 essential dynamics herbicide detoxification molecular dynamics protein engineering Abbreviations used: CDNB, 1-chloro-2,4-dinitrobenzene; G-site, GSH binding site; GST, glutathione S-transferase; H-site, electrophile binding site; Ni-NTA, Ni 2+ -nitrilotriacetate. Biochem...
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Biochem J (2001) 354 (2): 455–463.
Published: 22 February 2001
... properties of His- 311 are important for enzyme function. An important structural role has also been attributed to cis-Pro-288. This residue may provide the key residues Gln-287 and His-311 with the proper orientation for productive binding of formate. Key words: kinetic mechanism, protein engineering, site...
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Biochem J (2000) 352 (2): 257–266.
Published: 24 November 2000
... and R184 located close to the adenosine ribose 2«-phosphate group, and R144 likely to interact with the nicotinamide ribose 5«-phosphate group. Key words: coenzyme affinity, enzyme kinetics, flavodoxin NADP+ oxidoreductase, protein engineering. with other members of the ferredoxin reductase family...
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Biochem J (2000) 345 (3): 687–692.
Published: 25 January 2000
... biocatalysis dehydrogenases opiates protein engineering Biochem. J. (2000) 345, 687 692 (Printed in Great Britain) 687 Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation Edward H. WALKER1, Christopher E. FRENCH2, Deborah A. RATHBONE and Neil C. BRUCE3 Institute...
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Biochem J (2000) 345 (2): 247–254.
Published: 10 January 2000
... of the location of the ligand on the toxin and they were found to release the restrictocin fragment efficiently on proteolysis in Šitro. Key words: chimaeric toxins, protease, protein engineering, targeting, toxins. an intervening specific protease recognition site made them cytotoxic [12,13]. Chimaeric toxins...
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Biochem J (1999) 341 (1): 139–145.
Published: 24 June 1999
...). 4 1 1999 30 3 1999 23 4 1999 The Biochemical Society, London © 1999 1999 acceptor-binding affinity ammodytoxin enzyme activity protein engineering Vipera ammodytes ammodytes Biochem. J. (1999) 341, 139 145 (Printed in Great Britain) 139 An aromatic, but not a basic...
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Biochem J (1999) 339 (2): 309–317.
Published: 08 April 1999
... to denaturants such as guanidine HCl and urea was revealed; the wild-type protein always proved to be the most resistant. The results obtained show the importance of hydrogen bonds and ion pairs in determining protein stability and confirm that simulation methods are able to direct protein engineering in site...