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Articles

Chaetocin disrupts the SUV39H1–HP1 interaction independent of SUV39H1 methyltransferase activity

Linna Han, Jessica B. Lee, Elaine W. Indermaur, Albert J. Keung

Biochem J (2023) 480 (6): 421–432.

DOI: https://doi.org/10.1042/BCJ20220528

Published: 24 March 2023

... 2023 chaetocin epipolythiodixopiperazine heterochromatin histone methyltransferase protein–protein interaction yeast two-hybrid H3K9 methylation is a conserved modification across higher eukaryotes and is important in gene silencing and maintenance of constitutive heterochromatin...

View article titled, Chaetocin disrupts the SUV39H1–HP1 interaction independent of SUV39H1 methyltransferase activity

Includes: Supplementary data

Articles

New insights on the interaction mechanism of rhTNFα with its antagonists Adalimumab and Etanercept

María Angélica Contreras, Luis Macaya, Pedro Neira, Frank Camacho, Alaín González, Jannel Acosta, Raquel Montesino, Jorge Roberto Toledo, Oliberto Sánchez

Biochem J (2020) 477 (17): 3299–3311.

DOI: https://doi.org/10.1042/BCJ20200568

Published: 10 September 2020

..., the production of other inflammatory cytokines (IL-1, IL-6, IL-17), among others [ 6 ]. antibody protein purification protein–protein interaction tumour necrosis factors Although TNFα has an important physiological function in the immune response, it is known that the uncontrolled production...

View article titled, New insights on the interaction mechanism of rhTNFα with its antagonists Adalimumab and Etanercept

Articles

Molecular dynamics simulations and biochemical characterization of Pf 14-3-3 and Pf CDPK1 interaction towards its role in growth of human malaria parasite

Ravi Jain, Pinki Dey, Sakshi Gupta, Soumya Pati, Arnab Bhattacherjee, Manoj Munde, Shailja Singh

Biochem J (2020) 477 (12): 2153–2177.

DOI: https://doi.org/10.1042/BCJ20200145

Published: 22 June 2020

.... Correspondence: Shailja Singh ( shailja.jnu@gmail.com ) 18 2 2020 28 5 2020 2 6 2020 2 6 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 14-3-3 proteins peptides plasmodium falciparum protein–protein interaction...

View article titled, Molecular dynamics simulations and biochemical characterization of Pf 14-3-3 and Pf CDPK1 interaction towards its role in growth of human malaria parasite

Includes: Multimedia, Supplementary data

Articles

Structural and functional characterization of the RBBP4–ZNF827 interaction and its role in NuRD recruitment to telomeres

Sile F. Yang, Ai-ai Sun, Yunyu Shi, Fudong Li, Hilda A. Pickett

Biochem J (2018) 475 (16): 2667–2679.

DOI: https://doi.org/10.1042/BCJ20180310

Published: 31 August 2018

...–protein interaction RBBP4 zinc finger ZNF827 The nucleosome remodeling and histone deacetylase (NuRD; also called Mi-2) complex is a unique multi-subunit chromatin remodeling complex with both ATP-dependent chromatin remodeling and histone deacetylase activities [ 1 – 5 ]. NuRD comprises six...

View article titled, Structural and functional characterization of the RBBP4–ZNF827 interaction and its role in NuRD recruitment to telomeres

Articles

Structural complexity in the KCTD family of Cullin3-dependent E3 ubiquitin ligases

Daniel M. Pinkas, Caroline E. Sanvitale, Joshua C. Bufton, Fiona J. Sorrell, Nicolae Solcan, Rod Chalk, James Doutch, Alex N. Bullock

Biochem J (2017) 474 (22): 3747–3761.

DOI: https://doi.org/10.1042/BCJ20170527

Published: 01 November 2017

... a three-stranded β-sheet flanked by five α-helices [ 2 ]. Structural studies of soluble BTB proteins have since shown the BTB fold to be a highly versatile domain mediating protein–protein interactions in a large number of transcriptional repressors as well as E3 ubiquitin ligases [ 3 ]. © 2017...

View article titled, Structural complexity in the KCTD family of Cullin3-dependent E3 ubiquitin ligases

Includes: Supplementary data

Articles

γ-COPI mediates the retention of kAE1 G701D protein in Golgi apparatus – a mechanistic explanation of distal renal tubular acidosis associated with the G701D mutation

Natapol Duangtum, Mutita Junking, Suratchanee Phadngam, Nunghathai Sawasdee, Andrea Castiglioni, Komgrid Charngkaew, Thawornchai Limjindaporn, Ciro Isidoro, Pa-thai Yenchitsomanus

Biochem J (2017) 474 (15): 2573–2584.

DOI: https://doi.org/10.1042/BCJ20170088

Published: 18 July 2017

... 6 2017 23 6 2017 23 6 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 coat protein complex I distal renal tubular acidosis Golgi retention kidney anion exchanger 1 protein trafficking protein–protein interaction...

View article titled, γ-COPI mediates the retention of kAE1 G701D protein in Golgi apparatus – a mechanistic explanation of distal renal tubular acidosis associated with the G701D mutation

Articles

Macrocycles as protein–protein interaction inhibitors

Patrick G. Dougherty, Ziqing Qian, Dehua Pei

Biochem J (2017) 474 (7): 1109–1125.

DOI: https://doi.org/10.1042/BCJ20160619

Published: 15 March 2017

...Patrick G. Dougherty; Ziqing Qian; Dehua Pei Macrocyclic compounds such as cyclic peptides have emerged as a new and exciting class of drug candidates for inhibition of intracellular protein–protein interactions, which are challenging targets for conventional drug modalities (i.e. small molecules...

View article titled, Macrocycles as protein–protein interaction inhibitors

Articles

Identification of DNAJA1 as a novel interacting partner and a substrate of human transglutaminase 2

Elvan Ergülen, Bálint Bécsi, István Csomós, László Fésüs, Kajal Kanchan

Biochem J (2016) 473 (21): 3889–3901.

DOI: https://doi.org/10.1042/BCJ20160440

Published: 27 October 2016

... to its functional diversity [ 15 , 16 ]. It is well known that SLiMs and IDRs facilitate highly specific protein–protein interactions with moderate affinities; therefore, they are often exploited in signaling pathways [ 17 – 19 ]. Numerous SLiMs and 13 IDRs have been revealed in the TG2 structure...

View article titled, Identification of DNAJA1 as a novel interacting partner and a substrate of human transglutaminase 2

Includes: Supplementary data

Articles

Proximity-dependent biotin labelling in yeast using the engineered ascorbate peroxidase APEX2

Jiwon Hwang, Peter J. Espenshade

Biochem J (2016) 473 (16): 2463–2469.

DOI: https://doi.org/10.1042/BCJ20160106

Published: 11 August 2016

...Jiwon Hwang; Peter J. Espenshade The engineered ascorbate peroxidase (APEX2) has been effectively employed in mammalian cells to identify protein–protein interactions. APEX2 fused to a protein of interest covalently tags nearby proteins with biotin-phenol (BP) when H 2 O 2 is added to the cell...

View article titled, Proximity-dependent biotin labelling in yeast using the engineered ascorbate peroxidase APEX2

Includes: Supplementary data

Articles

AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA

Frank Götz, Yvette Roske, Maike Svenja Schulz, Karolin Autenrieth, Daniela Bertinetti, Katja Faelber, Kerstin Zühlke, Annika Kreuchwig, Eileen J. Kennedy, Gerd Krause, Oliver Daumke, Friedrich W. Herberg, Udo Heinemann, Enno Klussmann

Biochem J (2016) 473 (13): 1881–1894.

DOI: https://doi.org/10.1042/BCJ20160242

Published: 28 June 2016

... domain PKA-binding domain protein kinase A protein–protein interaction A-kinase-anchoring proteins (AKAPs) are a diverse family of around 50 scaffolding proteins with molecular masses ranging from 18 kDa to >500 kDa. They share the capacity to directly interact with protein kinase A (PKA...

View article titled, AKAP18:PKA-RIIα structure reveals crucial anchor points for recognition of regulatory subunits of PKA

Includes: Supplementary data

Articles

The interaction between AMPKβ2 and the PP1-targeting subunit R6 is dynamically regulated by intracellular glycogen content

Yvonne Oligschlaeger, Marie Miglianico, Vivian Dahlmans, Carla Rubio-Villena, Dipanjan Chanda, Maria Adelaida Garcia-Gimeno, Will A. Coumans, Yilin Liu, J. Willem Voncken, Joost J.F.P. Luiken, Jan F.C. Glatz, Pascual Sanz, Dietbert Neumann

Biochem J (2016) 473 (7): 937–947.

DOI: https://doi.org/10.1042/BJ20151035

Published: 29 March 2016

... transfections, HEK293T cells were seeded to 30% confluence in six-well plates (Greiner Bio-one) 24 h before transfection. For determination of protein–protein interactions, cells were co-transfected with AMPK (α1–myc, γ1 and β1-WT–mCherry or β2-WT/β2-T148D–mCherry), and/or FLAG–R6 (R6-WT/R6-RARA/R6-RAHA/R6...

View article titled, The interaction between AMPKβ2 and the PP1-targeting subunit R6 is dynamically regulated by intracellular glycogen content

Articles

A new level of regulation in gluconeogenesis: metabolic state modulates the intracellular localization of aldolase B and its interaction with liver fructose-1,6-bisphosphatase

Cristian A. Droppelmann, Doris E. Sáez, Joel L. Asenjo, Alejandro J. Yáñez, Mar García-Rocha, Ilona I. Concha, Manuel Grez, Joan J. Guinovart, Juan C. Slebe

Biochem J (2015) 472 (2): 225–237.

DOI: https://doi.org/10.1042/BJ20150269

Published: 13 November 2015

... different metabolic conditions and their protein–protein interaction in vitro and in vivo . We observed that glucose, insulin, glucagon and adrenaline differentially modulate the intracellular distribution of aldolase B and FBPase-1. Interestingly, the in vitro protein–protein interaction analysis between...

View article titled, A new level of regulation in gluconeogenesis: metabolic state modulates the intracellular localization of aldolase B and its interaction with liver fructose-1,6-bisphosphatase

Includes: Supplementary data

Articles

Identification of Leishmania donovani peroxin 14 residues required for binding the peroxin 5 receptor proteins

Hamed Hojjat, Armando Jardim

Biochem J (2015) 465 (2): 247–257.

DOI: https://doi.org/10.1042/BJ20141133

Published: 06 January 2015

... translocation protein–protein interaction The human protozoan parasite Leishmania is the causative agent of a spectrum of diseases known as leishmaniasis, a disease that currently affects ~12 million people in 88 countries in tropical and subtropical region of the globe [ 1 , 2 ]. Leishmania...

View article titled, Identification of Leishmania donovani peroxin 14 residues required for binding the peroxin 5 receptor proteins

Articles

Structural determinants of host specificity of complement Factor H recruitment by Streptococcus pneumoniae

David Achila, Aizhuo Liu, Rahul Banerjee, Yue Li, Erik Martinez-Hackert, Jing-Ren Zhang, Honggao Yan

Biochem J (2015) 465 (2): 325–335.

DOI: https://doi.org/10.1042/BJ20141069

Published: 06 January 2015

... specificity protein–protein interaction Streptococcus pneumoniae structural biology The complement system is a major component of vertebrate innate immunity and one of the most important frontline defenders against microbial invaders [ 1 , 2 ]. Uncontrolled or inappropriate complement activation...

View article titled, Structural determinants of host specificity of complement Factor H recruitment by Streptococcus pneumoniae

Includes: Supplementary data

Articles

Selenoprotein S is involved in maintenance and transport of multiprotein complexes

Anton A. Turanov, Valentina A. Shchedrina, Robert A. Everley, Alexei V. Lobanov, Sun Hee Yim, Stefano M. Marino, Steven P. Gygi, Dolph L. Hatfield, Vadim N. Gladyshev

Biochem J (2014) 462 (3): 555–565.

DOI: https://doi.org/10.1042/BJ20140076

Published: 22 August 2014

... complexes by anchoring them to the ER membrane. coiled-coil domain multiprotein complex protein–protein interaction selenocysteine selenoprotein S 1 These authors contributed equally to this work. 2 Present address: Nephrology Division, Department of Medicine, Massachusetts...

View article titled, Selenoprotein S is involved in maintenance and transport of multiprotein complexes

Articles

Recombinant form of mammalian gp91 phox is active in the absence of p22 phox

Aymen Ezzine, Hager Souabni, Tania Bizouarn, Laura Baciou

Biochem J (2014) 462 (2): 337–345.

DOI: https://doi.org/10.1042/BJ20131620

Published: 07 August 2014

...-dependent activation of gp91 phox occurs through multiple protein–protein interactions with cytosolic factors (p67 phox , p47 phox p40 phox and a small GTP-binding protein, Rac1/Rac2). Previous studies demonstrate that phagocytes from p22 phox -deficient patients have no detectable gp91 phox...

View article titled, Recombinant form of mammalian gp91 phox is active in the absence of p22 phox

Articles

The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron–sulfur cluster biogenesis and oxidative stress defence

Jan Rybniker, Florence Pojer, Jan Marienhagen, Gaëlle S. Kolly, Jeffrey M. Chen, Edeltraud van Gumpel, Pia Hartmann, Stewart T. Cole

Biochem J (2014) 459 (3): 467–478.

DOI: https://doi.org/10.1042/BJ20130732

Published: 11 April 2014

... protein IscU by protein–protein interaction leading to the formation of a [2Fe–2S] cluster in the presence of iron. Once two [2Fe–2S] clusters have been generated on an IscU dimer, an [4Fe–4S] cluster can be formed and transferred to Fe–S effector proteins or the A-type carrier protein IscA. This protein...

View article titled, The cysteine desulfurase IscS of Mycobacterium tuberculosis is involved in iron–sulfur cluster biogenesis and oxidative stress defence

Includes: Supplementary data

Articles

Multisite phosphorylation of 14-3-3 proteins by calcium-dependent protein kinases

Kirby N. Swatek, Rashaun S. Wilson, Nagib Ahsan, Rebecca L. Tritz, Jay J. Thelen

Biochem J (2014) 459 (1): 15–25.

DOI: https://doi.org/10.1042/BJ20130035

Published: 14 March 2014

... ]. 14-3-3 protein Arabidopsis calcium-dependent protein kinase kinase assay protein–protein interaction tyrosine phosphorylation To determine whether CPKs can phosphorylate 14-3-3 isoforms χ and ε, a series of kinase assays were performed using phylogenetically distinct CPKs (1, 3, 6, 8...

View article titled, Multisite phosphorylation of 14-3-3 proteins by calcium-dependent protein kinases

Includes: Supplementary data

Articles

Extended interaction network of procollagen C-proteinase enhancer-1 in the extracellular matrix

Romain Salza, Franck Peysselon, Emilie Chautard, Clément Faye, Laura Moschcovich, Tali Weiss, Laure Perrin-Cocon, Vincent Lotteau, Efrat Kessler, Sylvie Ricard-Blum

Biochem J (2014) 457 (1): 137–149.

DOI: https://doi.org/10.1042/BJ20130295

Published: 10 December 2013

... supernatants was measured using a Packard Tri-carb 1600 CA beta-counter. affinity extracellular matrix kinetics network protein–protein interaction surface plasmon resonance The effect of full-length PCPE-1 and its fragments on angiogenesis was investigated using an in vitro angiogenesis...

View article titled, Extended interaction network of procollagen C-proteinase enhancer-1 in the extracellular matrix

Includes: Supplementary data

Articles

Structural basis for phosphorylation-triggered autophagic clearance of Salmonella

Vladimir V. Rogov, Hironori Suzuki, Evgenij Fiskin, Philipp Wild, Andreas Kniss, Alexis Rozenknop, Ryuichi Kato, Masato Kawasaki, David G. McEwan, Frank Löhr, Peter Güntert, Ivan Dikic, Soichi Wakatsuki, Volker Dötsch

Biochem J (2013) 454 (3): 459–466.

DOI: https://doi.org/10.1042/BJ20121907

Published: 29 August 2013

...-associated protein light chain 3 beta (LC3) NMR spectroscopy optineurin protein–protein interaction selective autophagy X-ray crystallography All titration experiments were performed at 25°C using a VP-ITC microcalorimeter (MicroCal). The ITC data were analysed with the ITC-Origin 7.0...

View article titled, Structural basis for phosphorylation-triggered autophagic clearance of Salmonella

Includes: Supplementary data

Articles

Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46

Paul E. Pace, Alexander V. Peskin, Min-Hi Han, Mark B. Hampton, Christine C. Winterbourn

Biochem J (2013) 453 (3): 475–485.

DOI: https://doi.org/10.1042/BJ20130030

Published: 12 July 2013

... . The interaction was again observed, and displayed the same dependence on exposure to H 2 O 2 ( Figure 2 d). As other researchers have observed redox activity between PDIs and Prx4 [ 36 , 37 ], we investigated whether Prx4 could also form a protein–protein interaction with ERp46. We performed separate...

View article titled, Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46

Includes: Supplementary data

Articles

Pivotal role of P450–P450 interactions in CYP3A4 allostery: the case of α-naphthoflavone

Dmitri R. Davydov, Nadezhda Y. Davydova, Elena V. Sineva, Irina Kufareva, James R. Halpert

Biochem J (2013) 453 (2): 219–230.

DOI: https://doi.org/10.1042/BJ20130398

Published: 28 June 2013

... (LRET) microsome oligomerization protein–protein interaction α-naphthoflavone Analysis of series of spectra obtained in fluorescence spectroscopy experiments was done by PCA (principal component analysis) [ 35 , 36 ]. To resolve the changes in the fluorescence of the donor and acceptor...

View article titled, Pivotal role of P450–P450 interactions in CYP3A4 allostery: the case of α-naphthoflavone

Articles

Role of the unstructured N-terminal domain of the hAPE1 (human apurinic/apyrimidinic endonuclease 1) in the modulation of its interaction with nucleic acids and NPM1 (nucleophosmin)

Mattia Poletto, Carlo Vascotto, Pasqualina L. Scognamiglio, Lisa Lirussi, Daniela Marasco, Gianluca Tell

Biochem J (2013) 452 (3): 545–557.

DOI: https://doi.org/10.1042/BJ20121277

Published: 31 May 2013

... in this non-canonical function of the protein, serving as an adaptable device for protein–protein interactions and modulating its enzymatic activity on abasic DNA, as well as its binding to RNA molecules. Although many studies have thoroughly characterized the structure of the hAPE1 C-terminal globular domain...

View article titled, Role of the unstructured N-terminal domain of the hAPE1 (human apurinic/apyrimidinic endonuclease 1) in the modulation of its interaction with nucleic acids and NPM1 (nucleophosmin)

Includes: Supplementary data

Articles

Signal transfer in the plant plasma membrane: phospholipase A 2 is regulated via an inhibitory G α protein and a cyclophilin

Michael Heinze, Madeleine Herre, Carolin Massalski, Isabella Hermann, Udo Conrad, Werner Roos

Biochem J (2013) 450 (3): 497–509.

DOI: https://doi.org/10.1042/BJ20120793

Published: 28 February 2013

... membrane plant signalling protein–protein interaction 1 To whom correspondence should be addressed (email werner.roos@pharmazie.uni-halle.de ). The nucleotide sequence data of the Eschscholzia cyclophilin reported will appear in GenBank®, EMBL, DDBJ and GSDB Nucleotide Sequence...

View article titled, Signal transfer in the plant plasma membrane: phospholipase A 2 is regulated via an inhibitory G α protein and a cyclophilin

Includes: Supplementary data

Articles

Identification of transcriptional and phosphatase regulators as interaction partners of human ADA3, a component of histone acetyltransferase complexes

Sevil Zencir, Adam Sike, Melanie J. Dobson, Ferhan Ayaydin, Imre Boros, Zeki Topcu

Biochem J (2013) 450 (2): 311–320.

DOI: https://doi.org/10.1042/BJ20120452

Published: 15 February 2013

... of transcription regulatory mechanisms ADA-type adaptor proteins may participate in through protein–protein interactions. To understand how ADA3-containing complexes regulate the expression of specific genes, some of which are known to play direct roles in oncogenesis, and identify novel pathways affected by ADA3...

View article titled, Identification of transcriptional and phosphatase regulators as interaction partners of human ADA3, a component of histone acetyltransferase complexes

Articles

Protein–protein interactions between the bilirubin-conjugating UDPglucuronosyltransferase UGT1A1 and its shorter isoform 2 regulatory partner derived from alternative splicing

Mélanie Rouleau, Pierre Collin, Judith Bellemare, Mario Harvey, Chantal Guillemette

Biochem J (2013) 450 (1): 107–114.

DOI: https://doi.org/10.1042/BJ20121594

Published: 24 January 2013

... The Authors Journal compilation © 2013 Biochemical Society 2013 alternative splice product dominant-negative regulation membrane protein protein–protein interaction uridine diphosphate-glucuronosyltransferase (UGT) The functions of most proteins depend on continuous interactions with other...

View article titled, Protein–protein interactions between the bilirubin-conjugating UDPglucuronosyltransferase UGT1A1 and its shorter isoform 2 regulatory partner derived from alternative splicing

Articles

Molecular mechanisms underlying the interaction of protein phosphatase-1c with ASPP proteins

Tamara D. Skene-Arnold, Hue Anh Luu, R. Glen Uhrig, Veerle De Wever, Mhairi Nimick, Jason Maynes, Andrea Fong, Michael N. G. James, Laura Trinkle-Mulcahy, Greg B. Moorhead, Charles F. B. Holmes

Biochem J (2013) 449 (3): 649–659.

DOI: https://doi.org/10.1042/BJ20120506

Published: 09 January 2013

... to an SH3 protein domain. 29 3 2012 17 9 2012 23 10 2012 23 10 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 apoptosis-stimulating protein of p53 (ASPP) protein phosphatase-1 catalytic subunit (PP-1c) protein–protein interaction RVXF-binding motif...

View article titled, Molecular mechanisms underlying the interaction of protein phosphatase-1c with ASPP proteins

Includes: Supplementary data

Articles

Post-translational modification in the archaea: structural characterization of multi-enzyme complex lipoylation

Mareike G. Posner, Abhishek Upadhyay, Susan J. Crennell, Andrew J. A. Watson, Steve Dorus, Michael J. Danson, Stefan Bagby

Biochem J (2013) 449 (2): 415–425.

DOI: https://doi.org/10.1042/BJ20121150

Published: 14 December 2012

... in the nitrogen dimension, unless otherwise stated. binding-induced folding lipoate protein ligase lipoyl domain NMR spectroscopy protein–protein interaction X-ray crystallography Aerobic metabolism of 2-oxoacids and C1 metabolism are dependent on LA (lipoic acid) in a highly conserved manner...

View article titled, Post-translational modification in the archaea: structural characterization of multi-enzyme complex lipoylation

Articles

Glucan affinity of starch synthase IIa determines binding of starch synthase I and starch-branching enzyme IIb to starch granules

Fushan Liu, Nadya Romanova, Elizabeth A. Lee, Regina Ahmed, Martin Evans, Elliot P. Gilbert, Matthew K. Morell, Michael J. Emes, Ian J. Tetlow

Biochem J (2012) 448 (3): 373–387.

DOI: https://doi.org/10.1042/BJ20120573

Published: 21 November 2012

... causing major alterations to amylopectin architecture. The present study reports a biochemical and molecular analysis of an allelic variant of the sugary-2 mutation expressing a catalytically inactive form of SSIIa and sheds new light on its central role in protein–protein interactions and determination...

View article titled, Glucan affinity of starch synthase IIa determines binding of starch synthase I and starch-branching enzyme IIb to starch granules

Includes: Supplementary data

Articles

Cyanide is an adequate agonist of the plant hormone ethylene for studying signalling of sensor kinase ETR1 at the molecular level

Melanie M. A. Bisson, Georg Groth

Biochem J (2012) 444 (2): 261–267.

DOI: https://doi.org/10.1042/BJ20111447

Published: 11 May 2012

... binding. Replacement of Cys 65 in the ethylene-binding domain by serine dramatically reduced binding of radiolabelled cyanide. In contrast with wild-type ETR1, autokinase activity of the receptor is not reduced in the ETR1-C65S mutant upon addition of cyanide. Additionally, protein–protein interaction...

View article titled, Cyanide is an adequate agonist of the plant hormone ethylene for studying signalling of sensor kinase ETR1 at the molecular level

Includes: Supplementary data

Articles

RCD1–DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana

Julia P. Vainonen, Pinja Jaspers, Michael Wrzaczek, Airi Lamminmäki, Ramesha A. Reddy, Lauri Vaahtera, Mikael Brosché, Jaakko Kangasjärvi

Biochem J (2012) 442 (3): 573–581.

DOI: https://doi.org/10.1042/BJ20111739

Published: 24 February 2012

...–protein interaction protein stability transcription factor 2 Present address: Centre for Cellular and Molecular Biology, Hyderabad-500007, India. 3 To whom correspondence should be addressed ( jaakko.kangasjarvi@helsinki.fi ). 1 These authors contributed equally...

View article titled, RCD1–DREB2A interaction in leaf senescence and stress responses in Arabidopsis thaliana

Includes: Supplementary data

Articles

The interaction of the second Kunitz-type domain (KD2) of TFPI-2 with a novel interaction partner, prosaposin, mediates the inhibition of the invasion and migration of human fibrosarcoma cells

Chundi Xu, Fenge Deng, Zuohua Mao, Jing Zhang, Huijun Wang, Jiping Wang, Jingui Mu, Shanshan Deng, Duan Ma

Biochem J (2012) 441 (2): 665–674.

DOI: https://doi.org/10.1042/BJ20110533

Published: 21 December 2011

... for expression of an additional reporter gene, MEL1-encoding α-galactosidase, using X-α-Gal (5-bromo-4-chloroindol-3-yl α- D -galactopyranoside; Clontech). Double-positive clones were isolated and characterized by DNA sequencing. invasion Kunitz-type domain migration prosaposin protein–protein...

View article titled, The interaction of the second Kunitz-type domain (KD2) of TFPI-2 with a novel interaction partner, prosaposin, mediates the inhibition of the invasion and migration of human fibrosarcoma cells

Articles

Laforin, a dual-specificity phosphatase involved in Lafora disease, is phosphorylated at Ser 25 by AMP-activated protein kinase

Carlos Romá-Mateo, Maria del Carmen Solaz-Fuster, José Vicente Gimeno-Alcañiz, Vikas V. Dukhande, Jordi Donderis, Carolyn A. Worby, Alberto Marina, Olga Criado, Antonius Koller, Santiago Rodriguez De Cordoba, Matthew S. Gentry, Pascual Sanz

Biochem J (2011) 439 (2): 265–275.

DOI: https://doi.org/10.1042/BJ20110150

Published: 28 September 2011

... ). 24 1 2011 27 5 2011 28 6 2011 28 6 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 alanine scanning mutagenesis AMP-activated protein kinase (AMPK) glucan-phosphatase laforin phosphorylation protein–protein interaction Escherichia coli...

View article titled, Laforin, a dual-specificity phosphatase involved in Lafora disease, is phosphorylated at Ser 25 by AMP-activated protein kinase

Includes: Supplementary data

Articles

A phospholamban-tethered cardiac Ca 2+ pump reveals stoichiometry and dynamic interactions between the two proteins

Zhenhui Chen

Biochem J (2011) 439 (2): 313–319.

DOI: https://doi.org/10.1042/BJ20110926

Published: 28 September 2011

... to that of control samples co-expressing WT-SERCA2a and WT-PLB. Furthermore, even when covalently linked to SERCA2a, the PLB tether, identical with the free diffusing WT-PLB, retained its ability to form pentamers. cardiac Ca 2+ -ATPase enzyme regulation equilibrium phospholamban protein–protein interaction...

View article titled, A phospholamban-tethered cardiac Ca 2+ pump reveals stoichiometry and dynamic interactions between the two proteins

Articles

Monitoring protein–protein interactions in mammalian cells by trans -SUMOylation

Ratnesh K. Srivastav, Susan Schwede, Malte Klaus, Jessica Schwermann, Matthias Gaestel, Rainer Niedenthal

Biochem J (2011) 438 (3): 495–503.

DOI: https://doi.org/10.1042/BJ20110035

Published: 26 August 2011

...Ratnesh K. Srivastav; Susan Schwede; Malte Klaus; Jessica Schwermann; Matthias Gaestel; Rainer Niedenthal Protein–protein interactions are essential for almost all cellular processes, hence understanding these processes mainly depends on the identification and characterization of the relevant...

View article titled, Monitoring protein–protein interactions in mammalian cells by trans -SUMOylation

Includes: Supplementary data

Articles

Protein network study of human AF4 reveals its central role in RNA Pol II-mediated transcription and in phosphorylation-dependent regulatory mechanisms

Gabriella Esposito, Armando Cevenini, Alessandro Cuomo, Francesca de Falco, Dario Sabbatino, Fabrizio Pane, Margherita Ruoppolo, Francesco Salvatore

Biochem J (2011) 438 (1): 121–131.

DOI: https://doi.org/10.1042/BJ20101633

Published: 27 July 2011

... lymphoblastic leukaemia AF4–ENL–P-TEFb complex (AEP complex) AF4 interactome Mediator complex mixed-lineage leukaemia chimaera (MLL chimaera) protein–protein interaction RNA polymerase II-mediated transcription elongation Figure 1 Schematic representation of the flagged AF4 constructs used...

View article titled, Protein network study of human AF4 reveals its central role in RNA Pol II-mediated transcription and in phosphorylation-dependent regulatory mechanisms

Includes: Supplementary data

Articles

Divergent agonist selectivity in activating β 1 - and β 2 -adrenoceptors for G-protein and arrestin coupling

Ida Casella, Caterina Ambrosio, Maria Cristina Grò, Paola Molinari, Tommaso Costa

Biochem J (2011) 438 (1): 191–202.

DOI: https://doi.org/10.1042/BJ20110374

Published: 27 July 2011

... via β2-AR. Thus the structure of such ligands appears to induce an arrestin-interacting form of the receptor only when bound to the β1-AR subtype. β-adrenoceptor β-arrestin-2 G-protein-coupled receptor (GPCR) ligand efficacy protein–protein interaction resonance energy transfer...

View article titled, Divergent agonist selectivity in activating β 1 - and β 2 -adrenoceptors for G-protein and arrestin coupling

Includes: Supplementary data

Articles

NMR spectroscopy of 14-3-3ζ reveals a flexible C-terminal extension: differentiation of the chaperone and phosphoserine-binding activities of 14-3-3ζ

Danielle M. Williams, Heath Ecroyd, Katy L. Goodwin, Huanqin Dai, Haian Fu, Joanna M. Woodcock, Lixin Zhang, John A. Carver

Biochem J (2011) 437 (3): 493–503.

DOI: https://doi.org/10.1042/BJ20102178

Published: 13 July 2011

... characterization C-terminal flexibility molecular chaperone 14-3-3 protein protein aggregation protein–protein interaction All reagents used were of analytical grade and were obtained from Sigma–Aldrich unless otherwise specified. Unless otherwise stated, all solutions were prepared in 50 mM phosphate...

View article titled, NMR spectroscopy of 14-3-3ζ reveals a flexible C-terminal extension: differentiation of the chaperone and phosphoserine-binding activities of 14-3-3ζ

Articles

The phytotoxin fusicoccin promotes platelet aggregation via 14-3-3–glycoprotein Ib-IX-V interaction

Lorenzo Camoni, Cristina Di Lucente, Sabina Visconti, Patrizia Aducci

Biochem J (2011) 436 (2): 429–436.

DOI: https://doi.org/10.1042/BJ20102037

Published: 13 May 2011

... significance was assessed by unpaired Student's t test. All values are expressed as means±S.E.M. drug target fusicoccin glycoprotein Ib-IX-V platelet aggregation 14-3-3 protein protein–protein interaction receptor toxin FC (fusicoccin) ( Figure 1 A), a diterpene glucoside produced...

View article titled, The phytotoxin fusicoccin promotes platelet aggregation via 14-3-3–glycoprotein Ib-IX-V interaction

Articles

The remarkable diversity of plant PEPC (phosphoenolpyruvate carboxylase): recent insights into the physiological functions and post-translational controls of non-photosynthetic PEPCs

Brendan O'Leary, Joonho Park, William C. Plaxton

Biochem J (2011) 436 (1): 15–34.

DOI: https://doi.org/10.1042/BJ20110078

Published: 27 April 2011

... in our understanding of the post-translational control, protein–protein interactions and subcellular location of non-photosynthetic PEPCs. Figure 2 Models illustrating several metabolic functions of plant PEPC ( A ) Interactions between carbon and nitrogen metabolism involves three...

View article titled, The remarkable diversity of plant PEPC (phosphoenolpyruvate carboxylase): recent insights into the physiological functions and post-translational controls of non-photosynthetic PEPCs

Articles

A 2A adenosine receptor ligand binding and signalling is allosterically modulated by adenosine deaminase

Eduard Gracia, Kamil Pérez-Capote, Estefanía Moreno, Jana Barkešová, Josefa Mallol, Carme Lluís, Rafael Franco, Antoni Cortés, Vicent Casadó, Enric I. Canela

Biochem J (2011) 435 (3): 701–709.

DOI: https://doi.org/10.1042/BJ20101749

Published: 13 April 2011

... allosteric interaction G-protein-coupled receptor protein–protein interaction receptor binding parameter Self-association of proteins to form dimers and higher-order oligomers and/or interaction with other proteins are key factors in cell signalling [ 1 – 3 ]. A paradigmatic example are adenosine...

View article titled, A 2A adenosine receptor ligand binding and signalling is allosterically modulated by adenosine deaminase

Articles

Molecular interaction and functional regulation of connexin50 gap junctions by calmodulin

Yanyi Chen, Yubin Zhou, Xianming Lin, Hing-Cheung Wong, Qin Xu, Jie Jiang, Siming Wang, Monica M. Lurtz, Charles F. Louis, Richard D. Veenstra, Jenny J. Yang

Biochem J (2011) 435 (3): 711–722.

DOI: https://doi.org/10.1042/BJ20101726

Published: 13 April 2011

... junctional conductance protein–protein interaction The circulation of current fluid flow through the closely packed cells of the lens of the eye is essential for lens homoeostasis, and is highly dependent on functional cell–cell coupling. This is accomplished by gap junctions, relatively non...

View article titled, Molecular interaction and functional regulation of connexin50 gap junctions by calmodulin

Includes: Supplementary data

Articles

Targeting the regulation of CFTR channels

Paul D.W. Eckford, Christine E. Bear

Biochem J (2011) 435 (2): e1–e4.

DOI: https://doi.org/10.1042/BJ20110461

Published: 29 March 2011

... the idea that the cAMP-dependent channel activity of CFTR could be effectively enhanced by disruption of a protein–protein interaction which is normally inhibitory for the production of cAMP. This particular protein–protein interaction [between the PDZ motif of LPA 2 (type 2 lysophosphatidic acid receptor...

View article titled, Targeting the regulation of CFTR channels

Articles

Functional regulation of cystic fibrosis transmembrane conductance regulator-containing macromolecular complexes: a small-molecule inhibitor approach

Weiqiang Zhang, Himabindu Penmatsa, Aixia Ren, Chandanamali Punchihewa, Andrew Lemoff, Bing Yan, Naoaki Fujii, Anjaparavanda P. Naren

Biochem J (2011) 435 (2): 451–462.

DOI: https://doi.org/10.1042/BJ20101725

Published: 29 March 2011

... subcellular microdomains to modulate trafficking, transport and signalling in cells. Targeting protein–protein interactions within these macromolecular complexes would affect the expression or function of the CFTR channel. We specifically targeted the PDZ domain-based LPA 2 (type 2 lysophosphatidic acid...

View article titled, Functional regulation of cystic fibrosis transmembrane conductance regulator-containing macromolecular complexes: a small-molecule inhibitor approach

Includes: Supplementary data

Articles

RING domain dimerization is essential for RNF4 function

Chu Wai Liew, Huaiyu Sun, Tony Hunter, Catherine L. Day

Biochem J (2010) 431 (1): 23–29.

DOI: https://doi.org/10.1042/BJ20100957

Published: 14 September 2010

... E3 ligase protein domain protein–protein interaction really interesting new gene finger (RING finger) RING finger protein 4 (RNF4) small ubiquitin-like modifier (SUMO) ubiquitin RING (really interesting new gene) domain ubiquitin ligases promote the transfer of ubiquitin from ubiquitin...

View article titled, RING domain dimerization is essential for RNF4 function

Includes: Supplementary data

Articles

Identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X

Xiaoli Shi, Sandrine Opi, Adrien Lugari, Audrey Restouin, Thibault Coursindel, Isabelle Parrot, Javier Perez, Eric Madore, Pascale Zimmermann, Jacques Corbeil, Mingdong Huang, Stefan T. Arold, Yves Collette, Xavier Morelli

Biochem J (2010) 431 (1): 93–102.

DOI: https://doi.org/10.1042/BJ20100314

Published: 14 September 2010

.... Their functions are tightly regulated through SH (Src homology) domain-mediated protein–protein interactions. A yeast two-hybrid screen using SH3 domains as bait identified Alix [ALG-2 (apoptosis-linked gene 2)-interacting protein X] as a novel Hck (haemopoietic cell kinase) SH3 domain interactor. The Alix–Hck...

View article titled, Identification and biophysical assessment of the molecular recognition mechanisms between the human haemopoietic cell kinase Src homology domain 3 and ALG-2-interacting protein X

Includes: Supplementary data

Articles

14-3-3 proteins are promising LRRK2 interactors

Iakov N. Rudenko, Mark R. Cookson

Biochem J (2010) 430 (3): e5–e6.

DOI: https://doi.org/10.1042/BJ20101200

Published: 27 August 2010

... of complex proteins)] domain. As well as the two catalytic domains, LRRK2 possesses several protein–protein interaction domains, but their function and the proteins with which they interact are poorly understood. In this issue of the Biochemical Journal , Nichols et al. study the interaction of the N...

View article titled, 14-3-3 proteins are promising LRRK2 interactors

Articles

Amino acid residues in the β3 strand and subsequent loop of the conserved ETS domain that mediate basic leucine zipper (bZIP) recruitment and potentially distinguish functional attributes of Ets proteins

Toshibumi Shimokawa, Satoshi Nunomura, Yukinori Enomoto, Chisei Ra

Biochem J (2010) 430 (1): 129–139.

DOI: https://doi.org/10.1042/BJ20091742

Published: 28 July 2010

...Toshibumi Shimokawa; Satoshi Nunomura; Yukinori Enomoto; Chisei Ra Ets family members share a conserved DNA-binding ETS domain, and serve a variety of roles in development, differentiation and oncogenesis. Besides DNA binding, the ETS domain also participates in protein–protein interactions...

View article titled, Amino acid residues in the β3 strand and subsequent loop of the conserved ETS domain that mediate basic leucine zipper (bZIP) recruitment and potentially distinguish functional attributes of Ets proteins

Includes: Supplementary data

Articles

Transglutaminase-2: a new endostatin partner in the extracellular matrix of endothelial cells

Clément Faye, Antonio Inforzato, Marine Bignon, Daniel J. Hartmann, Laurent Muller, Lionel Ballut, Bjorn R. Olsen, Anthony J. Day, Sylvie Ricard-Blum

Biochem J (2010) 427 (3): 467–475.

DOI: https://doi.org/10.1042/BJ20091594

Published: 14 April 2010

.... endostatin (ES) extracellular matrix protein–protein interaction surface plasmon resonance (SPR) binding assays transglutaminase-2 (TG-2) Endostatin is a C-terminal fragment of the α1 chain of collagen XVIII, which inhibits angiogenesis and tumour growth [ 1 , 2 ]. It binds to the α5β1 and αvβ3...

View article titled, Transglutaminase-2: a new endostatin partner in the extracellular matrix of endothelial cells

Includes: Supplementary data

Articles

Redox-mediated interactions of VHb ( Vitreoscilla haemoglobin) with OxyR: novel regulation of VHb biosynthesis under oxidative stress

Arvind Anand, Brian T. Duk, Sandeep Singh, Meltem Y. Akbas, Dale A. Webster, Benjamin C. Stark, Kanak L. Dikshit

Biochem J (2010) 426 (3): 271–280.

DOI: https://doi.org/10.1042/BJ20091417

Published: 24 February 2010

...@imtech.res.in ). 10 9 2009 17 12 2009 22 12 2009 22 12 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 bacterial haemoglobin oxidative stress OxyR protein–protein interaction Vitreoscilla haemoglobin (VHb) Vitreoscilla Haemoglobins...

View article titled, Redox-mediated interactions of VHb ( Vitreoscilla haemoglobin) with OxyR: novel regulation of VHb biosynthesis under oxidative stress

Includes: Supplementary data

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