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Keywords: phosphorylation
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Biochem J (2022) 479 (18): 1917–1940.
Published: 23 September 2022
...-regulation of the phosphorylation and thus the activity of its essential downstream effector the AGC family protein kinase Ypk1. Through the ensuing effect on the efficiency with which Ypk1 phosphorylates multiple substrates that control diverse processes, membrane homeostasis is maintained. Thus, the major...
Includes: Supplementary data
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Biochem J (2022) 479 (17): 1877–1889.
Published: 16 September 2022
... into the membrane after trafficking through the parasite secretory system as soluble, chaperoned complexes. A regulator of this process is an atypical protein kinase called WNG1. Phosphorylation by WNG1 appears to serve as a switch for membrane integration. However, like its substrates, WNG1 is secreted from...
Includes: Supplementary data
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Biochem J (2022) 479 (9): 929–951.
Published: 06 May 2022
... by caspase-8, RIP1 autophosphorylation, and phosphorylation by a number of signaling kinases can greatly impact cellular fate. Disruption of the tightly regulated RIP1 modifications can lead to signaling disbalance in TNF and/or TLR controlled and other inflammatory pathways, and result in severe human...
Articles
Biochem J (2022) 479 (6): 751–766.
Published: 28 March 2022
.... Parkin activation involves binding of a phosphorylated ubiquitin (pUb), followed by phosphorylation of the Ubl domain in parkin, both mediated by the OMM kinase, PINK1. How an OMM protein is selected for ubiquitination is unclear. Parkin targeted OMM proteins have little structural or sequence similarity...
Includes: Supplementary data
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Biochem J (2021) 478 (14): 2811–2823.
Published: 23 July 2021
... conformational changes enzyme–substrate interactions phosphorylation small-molecule inhibitor ULK kinase Membrane abscission is the ultimate step in several physiologically important processes such as exosome budding and cytokinesis [ 1 ]. Any connection between two membrane substructures is cut...
Includes: Supplementary data
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Biochem J (2021) 478 (3): 553–578.
Published: 10 February 2021
... mutations in LRRK2 that enhance kinase activity cause Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases including Rab8A and Rab10 within its effector binding motif. Here, we explore whether LRRK1, a less studied homolog of LRRK2 that regulates growth factor receptor trafficking...
Includes: Supplementary data
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Biochem J (2021) 478 (1): 135–156.
Published: 13 January 2021
... of the Biochemical Society 2021 DNA damage signaling mediators phosphorylation sensors ubiquitylation The integrity of the genetic material in living organisms inevitably suffers from deleterious attacks from extrinsic and intrinsic sources including ionizing radiation (IR) and reactive oxygen...
Articles
Biochem J (2020) 477 (23): 4491–4513.
Published: 03 December 2020
...-based phosphoproteomics in WRL68 cells showed that the oxidative damage induced by H 2 O 2 increased the phosphorylation of YAP1, a transcriptional co-activator involved in cell survival, and modified the phosphorylation of other proteins involved in transcription. Genetic or pharmacological inhibition...
Includes: Supplementary data
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Biochem J (2020) 477 (18): 3583–3598.
Published: 28 September 2020
... HepG2 cells cultured in high glucose (450 mg/dl) medium. Here, we have investigated this glucose signal that leads to phosphorylation of nuclear receptor RORα (NR1F1) at Ser100 and the transcription mechanism by which phosphorylated RORα transduces this signal to nuclear receptor HNF4α, activating...
Includes: Supplementary data
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Biochem J (2020) 477 (12): 2237–2248.
Published: 24 June 2020
... substrate. AMPK directly phosphorylated SRSF1 at Ser133 in an RNA recognition motif. Ser133 phosphorylation suppressed the interaction between SRSF1 and specific RNA sequences without altering the subcellular localization of SRSF1. Moreover, AMPK regulated the SRSF1-mediated alternative pre-mRNA splicing...
Articles
Biochem J (2020) 477 (7): 1219–1225.
Published: 09 April 2020
...Nikolai N. Sluchanko Many major protein–protein interaction networks are maintained by ‘hub’ proteins with multiple binding partners, where interactions are often facilitated by intrinsically disordered protein regions that undergo post-translational modifications, such as phosphorylation...
Articles
Biochem J (2019) 476 (18): 2561–2577.
Published: 20 September 2019
... degradation protein 1 (Ufd1) with VCP via its SHP box motif ( 228 F-S-G-S-G-N-R-L 235 ) is required for ERAD. However, the mechanisms by which the VCP–Ufd1 interaction is regulated are not well understood. Here, we found that the serine 229 residue located in the Ufd1 SHP box is phosphorylated in vitro...
Articles
Biochem J (2019) 476 (17): 2499–2514.
Published: 13 September 2019
... with ROCK1 is via its Rho domain. The Rho domain binds to the coiled-coil region of ROCK1 close to its kinase domain. We identify two amino acids within the Rho domain that alter RhoBTB1 association with ROCK1. RhoBTB1 is a substrate for ROCK1, and mutation of putative phosphorylation sites reduces its...
Includes: Supplementary data
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Biochem J (2019) 476 (13): 1911–1926.
Published: 02 July 2019
... License 4.0 (CC BY) . ERK mass spectrometry phosphorylation quality control tyrosine nitration ubiquitination The mitogen-activate protein kinase ERKs (extracellular-regulated protein kinases) play a central role in various cellular processes, including proliferation, migration...
Includes: Supplementary data
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Biochem J (2018) 475 (21): 3331–3357.
Published: 09 November 2018
... are fundamental to human health [ Biochimica et Biophysica Acta (2007) 1768 , 994–1005] and are the target of >30% of pharmaceuticals in clinical use [ Biotechnology Advances (2013) 31 , 1676–1694; Nature Reviews Drug Discovery (2017) 16 , 829–842]. This review focuses on phosphorylation of G protein subunits...
Includes: Supplementary data
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Biochem J (2018) 475 (16): 2699–2712.
Published: 31 August 2018
... to undergo phosphorylation, but the impact of this on function has not been investigated. Here, we show for the first time that IPO13 is phosphorylated by cAMP-dependent protein kinase A specifically at serine 193. Results from fluorescence recovery after photobleaching and fluorescence loss...
Articles
Biochem J (2018) 475 (11): 1861–1883.
Published: 06 June 2018
... repeat protein kinase-2) and VPS35 genes result in autosomal dominant Parkinson's disease. The VPS35 gene encodes for the cargo-binding component of the retromer complex, while LRRK2 modulates vesicular trafficking by phosphorylating a subgroup of Rab proteins. Pathogenic mutations in LRRK2 increase its...
Includes: Supplementary data
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Biochem J (2018) 475 (9): 1583–1595.
Published: 09 May 2018
... lacking five O-glycosylation sites (Thr 134 /Thr 138 /Thr 143 /Thr 147 /Thr 152 ) in the threonine/proline-rich region increased cell adhesion activity and phosphorylation compared with the wild type. However, the role of O-glycosylation in cell adhesion activity and phosphorylation of OPN remains...
Includes: Supplementary data
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Biochem J (2018) 475 (5): 981–1002.
Published: 15 March 2018
... interactions with proteins involved in chromatin and gene expression but retained interactions with HMMR and its known partners including CHICA. By analyzing BACH1 modification using stable isotope labeling with amino acids in cell culture, mitosis-specific phosphorylations of BACH1 were observed...
Articles
Biochem J (2018) 475 (1): 185–189.
Published: 05 January 2018
... (LRRK2) is a Ser/Thr kinase linked to familial and sporadic cases of Parkinson's disease (PD). Recent work established that multiple Rab GTPases are physiological substrates of LRRK2, with Rab10 in particular emerging as a human substrate whose site-specific phosphorylation mirrors hyperactive LRRK2...
Articles
Biochem J (2017) 474 (23): 3963–3984.
Published: 21 November 2017
... the conserved signaling features of DCBLD family members that drive their molecular activities. We previously identified DCBLD2 tyrosine phosphorylation sites in intracellular YxxP motifs that are required for the phosphorylation-dependent binding of the signaling adaptors CRK and CRKL (CT10 regulator of kinase...
Includes: Supplementary data
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Biochem J (2017) 474 (15): 2547–2562.
Published: 18 July 2017
... inactivated by RSS via polysulfidation of the active-site Cys residue. CaMKIV is phosphorylated at Thr 196 by its upstream CaMK kinase (CaMKK), resulting in the induction of its full activity. In vitro incubation of CaMKIV with the exogenous RSS donors Na 2 S n ( n = 2–4) resulted in dose-dependent inhibition...
Includes: Supplementary data
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Biochem J (2017) 474 (14): 2449–2464.
Published: 11 July 2017
... and its implications for viral replication have not been well studied. Here, we show that the Mdm2 protein level increases during HIV-1 infection and this effect is mediated by HIV-1 Tat protein. Tat appears to stabilise Mdm2 at the post-translational level by inducing its phosphorylation at serine-166...
Includes: Supplementary data
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Biochem J (2017) 474 (5): 699–713.
Published: 20 February 2017
... trafficking and cancer cell proliferation. CDK16 is activated through binding to cyclin Y via a phosphorylation-dependent 14-3-3 interaction and has a unique consensus substrate phosphorylation motif compared with conventional CDKs. To elucidate the structure and inhibitor-binding properties of this atypical...
Includes: Supplementary data
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Biochem J (2017) 474 (5): 683–697.
Published: 20 February 2017
... highly conserved phosphorylation sites (T110, S119, S231, S233 and S251) in PRRXL1 primary structure. Four are phospho-S/T-P sites, which suggest a role for the prolyl isomerase PIN1 in regulating PRRXL1. Accordingly, PRRXL1 physically interacts with PIN1 and displays diminished transcriptional activity...
Articles
Biochem J (2016) 473 (22): 4173–4192.
Published: 10 November 2016
... and kidney where it modulates extracellular Ca 2+ homeostasis and bone turnover. It is well established that phosphorylation of GPCRs constitutes a key event in regulating receptor function by promoting arrestin recruitment and coupling to G-protein-independent signaling pathways. Mapping phosphorylation...
Articles
Biochem J (2016) 473 (7): 937–947.
Published: 29 March 2016
... the glucose-induced inactivation of AMPK. In the present study, we further investigated the interaction of R6 with AMPKβ and the possible dependency on Thr-148 phosphorylation status. Yeast two-hybrid (Y2H) analyses and co-immunoprecipitation (IP) of the overexpressed proteins in human embryonic kidney (HEK...
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Biochem J (2016) 473 (4): 509–523.
Published: 09 February 2016
... that aPKC is constitutively phosphorylated and, using a genetically-encoded reporter for PKC activity, basally active in cells. Specifically, we show that phosphorylation at two key regulatory sites, the activation loop and turn motif, of the aPKC PKCζ in multiple cultured cell types is constitutive...
Articles
Biochem J (2016) 473 (3): 311–320.
Published: 25 January 2016
... phosphorylation [Gong et al. (2015) Mol. Cell. Biol. 35 , 1727–1740]. The present study uses MS-based methods to identify PKCδ phosphorylation at Thr 50 and Ser 645 (in resting and PMA-treated cardiomyocytes) as well as Thr 37 , Thr 38 , Ser 130 , Thr 164 , Thr 211 , Thr 215 , Ser 218 , Thr 295 , Ser 299 and Thr...
Articles
Biochem J (2015) 472 (3): 329–338.
Published: 27 November 2015
...Malik M. Keshwani; Kendra L. Hailey; Brandon E. Aubol; Laurent Fattet; Maria L. McGlone; Patricia A. Jennings; Joseph A. Adams Phosphorylation-dependent cell communication requires enzymes that specifically recognize key proteins in a sea of similar, competing substrates. The protein kinases...
Articles
Biochem J (2015) 472 (1): 17–32.
Published: 30 October 2015
... fragments (MoRFs), which thereby undergo local disorder-to-order transitions. Their conformations and associations are modulated by environment and by a dynamic barcode of post-translational modifications, particularly phosphorylation by mitogen-activated and other protein kinases and deimination...
Articles
Biochem J (2015) 470 (1): 77–90.
Published: 06 August 2015
... exclusively to the apical domain. Furthermore, phosphorylation of Ser 303 located in the EBRS of NHERF2, decreases the binding affinity for ezrin, dislocates apical NHERF2 into the cytosol and increases the NHERF2 microvillar mobility rate. Moreover, increased phosphorylation of Ser 303 was functionally...
Articles
Biochem J (2015) 468 (1): 177–190.
Published: 05 May 2015
... and phosphorylation. However, whether Akt/PKB (protein kinase) regulates SERT function is not known. In the present study, we made the novel observation that inhibition of Akt resulted in the down-regulation of SERT function through the regulation of SERT trafficking and phosphorylation. Akt inhibitor Akt X {10-(4...
Articles
Biochem J (2015) 466 (2): 311–322.
Published: 20 February 2015
... in arginine–serine dipeptide repeats (RS domains). Although the SRPKs (SR-specific protein kinases) phosphorylate these repeats, RS domains also contain prolines with flanking serines that are phosphorylated by a second family of protein kinases known as the CLKs (Cdc2-like kinases). The role of specific...
Includes: Supplementary data
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Biochem J (2014) 464 (3): 323–334.
Published: 05 December 2014
.... The activities of glycogen synthase and glycogen phosphorylase, the rate-limiting enzymes of the synthesis and degradation processes, respectively, are regulated by allosteric modulation and reversible phosphorylation. To identify the protein kinases affecting glycogen metabolism in Neurospora crassa , we...
Includes: Supplementary data
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Biochem J (2014) 464 (1): 145–156.
Published: 23 October 2014
... phosphorylates two distinct sites in the N-terminus of PI4KIIα (Ser 5 and Ser 47 ), promoting binding to the adaptor protein 3 (AP-3) complex for trafficking to the lysosome to be degraded. Blocking phosphorylation reduces trafficking to the lysosome, stabilizing PI4KIIα and its cargo proteins for redistribution...
Articles
Biochem J (2014) 463 (1): 93–102.
Published: 08 September 2014
... and adhesion primarily through its association with several integrins such as αvβ3, and its phosphorylation affects these processes. However, the mechanism by which OPN O -glycosylation affects these processes is not completely understood. In the present study, we demonstrated that OPN O -glycosylation self...
Includes: Supplementary data
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Biochem J (2014) 463 (1): e1–e2.
Published: 08 September 2014
..., activation of IKKβ requires phosphorylation of the activation loop of its kinase domain. Different upstream protein kinases, and IKKβ itself, have been reported to directly phosphorylate and activate IKKβ in vitro , but the exact molecular mechanism of IKKβ activation in cells has remained unclear...
Articles
Biochem J (2014) 462 (3): 567–579.
Published: 22 August 2014
...Clara Andrea Solari; Vanesa Tudisca; Marcelo Pugliessi; Alejandro Daniel Nadra; Silvia Moreno; Paula Portela PKA (cAMP-dependent protein kinase) activity, as well as that of other AGC members, is regulated by multiple phosphorylations of its catalytic subunits. In Saccharomyces cerevisiae , the PKA...
Includes: Supplementary data
Articles
Biochem J (2014) 462 (1): 143–152.
Published: 24 July 2014
...Brandon E. Aubol; Ryan M. Plocinik; Malik M. Keshwani; Maria L. McGlone; Jonathan C. Hagopian; Gourisankar Ghosh; Xiang-Dong Fu; Joseph A. Adams SR proteins are essential splicing factors that are regulated through multisite phosphorylation of their RS (arginine/serine-rich) domains by two major...
Includes: Supplementary data
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Biochem J (2014) 460 (3): 399–410.
Published: 29 May 2014
... that CD2 co-stimulation induces phosphorylation of the TCR-proximal signalling complex, whereas CD28 activates distal signalling molecules, including the transcription factors NF-κB (nuclear factor κB), ATF (activating transcription factor)-2, STAT3/5 (signal transducer and activator of transcription 3/5...
Includes: Supplementary data
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Biochem J (2014) 460 (2): 165–175.
Published: 13 May 2014
... phosphorylation protein kinase transient receptor potential melastatin (TRPM) TRPM6 (transient receptor potential melastatin 6) is an exceptional enzyme possessing a Mg 2+ -permeant ion channel domain and a C-terminal protein kinase moiety [ 1 – 4 ]. Autosomal recessive mutations in the TRPM6 gene...
Includes: Supplementary data
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Biochem J (2014) 460 (1): 127–141.
Published: 25 April 2014
...) is activated by mitochondrial depolarization and stimulates the Parkin E3 ligase by phosphorylating Ser 65 within its Ubl (ubiquitin-like) domain. Using phosphoproteomic analysis, we identified a novel ubiquitin phosphopeptide phosphorylated at Ser 65 that was enriched 14-fold in HEK (human embryonic kidney...
Includes: Supplementary data
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Biochem J (2014) 459 (3): 441–453.
Published: 11 April 2014
... expressed in DRG and dSC (dorsal spinal cord) nociceptive neurons. PRRXL1 is crucial for the establishment and maintenance of nociceptive circuitry, as Prrxl1 −/− mice present neuronal loss, reduced pain sensitivity and failure to thrive. In the present study, we show that PRRXL1 is highly phosphorylated...
Includes: Supplementary data
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Biochem J (2014) 459 (2): 251–263.
Published: 28 March 2014
...Shingo Kasamatsu; Yasuo Watanabe; Tomohiro Sawa; Takaaki Akaike; Hideshi Ihara Phosphorylation is considered a main mechanism modulating nNOS (neuronal nitric oxide synthase) function to reduce NO production. In the present study, the effects of nNOS phosphorylation on redox signalling, including...
Articles
Biochem J (2014) 459 (1): 193–203.
Published: 14 March 2014
... enzymes. To date, little is known about the upstream regulation of hPXR. Using MS analysis and a kinome-wide siRNA screen, we report that the E3 ligase UBR5 (ubiquitin protein ligase E3 component n-recognin 5) and DYRK2 (dual-specificity tyrosine-phosphorylation-regulated kinase 2) regulate hPXR stability...
Includes: Supplementary data
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Biochem J (2014) 458 (2): 313–322.
Published: 14 February 2014
... the targets of calcium-dependent phosphorylation within the stromal proteome. A 73 kDa protein was identified as one of the most dominant proteins undergoing phosphorylation in a calcium-dependent manner in the stromal extracts of both Arabidopsis and Pisum . It was identified as TKL (transketolase...
Includes: Supplementary data
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Biochem J (2014) 458 (2): 395–405.
Published: 14 February 2014
...-kinase (JH2-JH1) exhibited comparable catalytic activities and inhibition by SOCS3 in vitro . These data were consistent with SAXS, in which the JH2 and JH1 domains behaved independently in the absence of receptor association. V617F JAK2 Janus kinase phosphorylation pseudokinase small-angle X...
Includes: Supplementary data
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Biochem J (2013) 456 (1): 119–128.
Published: 24 October 2013
...Evy Lobbestael; Jing Zhao; Iakov N. Rudenko; Aleksandra Beylina; Fangye Gao; Justin Wetter; Monique Beullens; Mathieu Bollen; Mark R. Cookson; Veerle Baekelandt; R. Jeremy Nichols; Jean-Marc Taymans A cluster of phosphorylation sites in LRRK2 (leucine-rich repeat kinase 2), including Ser 910 , Ser...
Includes: Supplementary data
Articles
Biochem J (2013) 454 (1): 39–47.
Published: 26 July 2013
...Jianchuan Wang; Chen Zhong; Fang Wang; Fangfang Qu; Jianping Ding The activity of S6K1 (p70 ribosomal protein subunit 6 kinase 1) is stimulated by phosphorylation of Thr 389 in the hydrophobic motif by mTORC1 (mammalian target of rapamycin complex 1) and phosphorylation of Thr 229 in the activation...
Includes: Supplementary data