Page 1 | Search Results | Biochemical Journal

1-45 of 45

Sort by

Sort Order Select

Articles

The WW domain of IQGAP1 binds directly to the p110α catalytic subunit of PI 3-kinase

A. Jane Bardwell, Madhuri Paul, Kiku C. Yoneda, María D. Andrade-Ludeña, Oanh T. Nguyen, David A. Fruman, Lee Bardwell

Biochem J (2023) 480 (10): 729–750.

https://doi.org/10.1042/BCJ20220493

Published: 26 May 2023

... from human cells, we show that the WW domain of human IQGAP1 binds directly to the p110α catalytic subunit of phosphoinositide 3-kinase (PI3K). In contrast, the WW domain does not bind to ERK1/2, MEK1/2, or the p85α regulatory subunit of PI3K when p85α is expressed alone. However, the WW domain is able...

View article titled, The WW domain of IQGAP1 binds directly to the p110α catalytic subunit of PI 3-kinase

Articles

Macropinocytosis: mechanisms and regulation

Gilbert Salloum, Anne R. Bresnick, Jonathan M. Backer

Biochem J (2023) 480 (5): 335–362.

https://doi.org/10.1042/BCJ20210584

Published: 15 March 2023

... phosphoinositide 3-kinase Rac Macropinocytosis is a common cellular process in eukaryotic cells that generates large intracellular vesicles (macropinosomes) that can range from 0.2–5 µm in diameter [ 1 ]. Depending on the cell type, macropinocytosis can be constitutive or ligand regulated. Given the wide...

View article titled, Macropinocytosis: mechanisms and regulation

Includes: Multimedia, Supplementary data

Articles

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44

Nazma Malik, Raja S. Nirujogi, Julien Peltier, Thomas Macartney, Melanie Wightman, Alan R. Prescott, Robert Gourlay, Matthias Trost, Dario R. Alessi, Athanasios Karapetsas

Biochem J (2019) 476 (20): 3081–3107.

https://doi.org/10.1042/BCJ20190608

Published: 30 October 2019

... of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . Akt phosphoinositide 3-kinase phosphoproteomics SGK3 syntaxins The phosphoinositide 3-kinase (PI3K) pathway plays a crucial role in various biological processes, including cell growth...

View article titled, Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44

Includes: Supplementary data

Articles

Zinc induces iron uptake and DMT1 expression in Caco-2 cells via a PI3K/IRP2 dependent mechanism

Palsa Kondaiah, Mohamad F. Aslam, Purna Chandra Mashurabad, Paul A. Sharp, Raghu Pullakhandam

Biochem J (2019) 476 (11): 1573–1583.

https://doi.org/10.1042/BCJ20180939

Published: 11 June 2019

... 2019 15 5 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 gastrointestinal physiology iron metabolism nutrition phosphoinositide 3-kinase zinc Iron and zinc deficiencies coexist in populations subsisting on phytic...

View article titled, Zinc induces iron uptake and DMT1 expression in Caco-2 cells via a PI3K/IRP2 dependent mechanism

Includes: Supplementary data

Articles

Understanding phosphoinositides: rare, dynamic, and essential membrane phospholipids

In Collection

Editors' pick of review article highlights published in the Biochemical Journal in 2018 & 2019

Eamonn J. Dickson, Bertil Hille

Biochem J (2019) 476 (1): 1–23.

https://doi.org/10.1042/BCJ20180022

Published: 07 January 2019

...). Published by Portland Press Limited on behalf of the Biochemical Society 2019 lipid transfer phosphatidylinositol phosphoinositide 3-kinase PTEN transmembrane proteins PPIs (polyphosphoinositides) are reversibly phosphorylated derivatives of the membrane phospholipid phosphatidylinositol...

View article titled, Understanding phosphoinositides: rare, dynamic, and essential membrane phospholipids

Articles

Mechanism of activation of SGK3 by growth factors via the Class 1 and Class 3 PI3Ks

Nazma Malik, Thomas Macartney, Annika Hornberger, Karen E. Anderson, Hannah Tovell, Alan R. Prescott, Dario R. Alessi

Biochem J (2018) 475 (1): 117–135.

https://doi.org/10.1042/BCJ20170650

Published: 02 January 2018

... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . insulin-like growth factor kinases phosphoinositide 3-kinase SGK3 (serum- and glucocorticoid-regulated kinase family member 3) belongs to the AGC family...

View article titled, Mechanism of activation of SGK3 by growth factors via the Class 1 and Class 3 PI3Ks

Includes: Supplementary data

Articles

Rac1-stimulated macropinocytosis enhances Gβγ activation of PI3Kβ

Zahra Erami, Bassem D. Khalil, Gilbert Salloum, Yanhua Yao, Jaclyn LoPiccolo, Aliaksei Shymanets, Bernd Nürnberg, Anne R. Bresnick, Jonathan M. Backer

Biochem J (2017) 474 (23): 3903–3914.

https://doi.org/10.1042/BCJ20170279

Published: 16 November 2017

...Zahra Erami; Bassem D. Khalil; Gilbert Salloum; Yanhua Yao; Jaclyn LoPiccolo; Aliaksei Shymanets; Bernd Nürnberg; Anne R. Bresnick; Jonathan M. Backer Phosphoinositide 3-kinases (PI 3-kinases) are regulated by a diverse range of upstream activators, including receptor tyrosine kinases (RTKs), G...

View article titled, Rac1-stimulated macropinocytosis enhances Gβγ activation of PI3Kβ

Articles

Combining properties of different classes of PI3Kα inhibitors to understand the molecular features that confer selectivity

Grace Q. Gong, Jackie D. Kendall, James M.J. Dickson, Gordon W. Rewcastle, Christina M. Buchanan, William A. Denny, Peter R. Shepherd, Jack U. Flanagan

Biochem J (2017) 474 (13): 2261–2276.

https://doi.org/10.1042/BCJ20161098

Published: 26 June 2017

...Grace Q. Gong; Jackie D. Kendall; James M.J. Dickson; Gordon W. Rewcastle; Christina M. Buchanan; William A. Denny; Peter R. Shepherd; Jack U. Flanagan Phosphoinositide 3-kinases (PI3Ks) are major regulators of many cellular functions, and hyperactivation of PI3K cell signalling pathways is a major...

View article titled, Combining properties of different classes of PI3Kα inhibitors to understand the molecular features that confer selectivity

Includes: Supplementary data

Articles

The intricate regulation and complex functions of the Class III phosphoinositide 3-kinase Vps34

Jonathan M. Backer

Biochem J (2016) 473 (15): 2251–2271.

https://doi.org/10.1042/BCJ20160170

Published: 28 July 2016

...Jonathan M. Backer The Class III phosphoinositide 3-kinase Vps34 (vacuolar protein sorting 34) plays important roles in endocytic trafficking, macroautophagy, phagocytosis, cytokinesis and nutrient sensing. Recent studies have provided exciting new insights into the structure and regulation...

View article titled, The intricate regulation and complex functions of the Class III phosphoinositide 3-kinase Vps34

Articles

Physiological expression of the PI3K-activating mutation Pik3ca H1047R combines with Apc loss to promote development of invasive intestinal adenocarcinomas in mice

Lauren M. Hare, Toby J. Phesse, Paul M. Waring, Karen G. Montgomery, Kathryn M. Kinross, Kevin Mills, Vincent Roh, Joan K. Heath, Robert G. Ramsay, Matthias Ernst, Wayne A. Phillips

Biochem J (2014) 458 (2): 251–258.

https://doi.org/10.1042/BJ20131412

Published: 14 February 2014

...Lauren M. Hare; Toby J. Phesse; Paul M. Waring; Karen G. Montgomery; Kathryn M. Kinross; Kevin Mills; Vincent Roh; Joan K. Heath; Robert G. Ramsay; Matthias Ernst; Wayne A. Phillips PIK3CA , the gene encoding the p110α catalytic subunit of PI3K (phosphoinositide 3-kinase), is mutated...

View article titled, Physiological expression of the PI3K-activating mutation Pik3ca H1047R combines with Apc loss to promote development of invasive intestinal adenocarcinomas in mice

Includes: Supplementary data

Articles

The special systems biology of the sperm

Ravinder Anand-Ivell, Richard Ivell

Biochem J (2011) 436 (3): e3–e5.

https://doi.org/10.1042/BJ20110766

Published: 27 May 2011

... 2011 5 5 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 apoptosis compartmentalization phosphoinositide 3-kinase spermatozoa The article by Koppers et al. [ 1 ] has highlighted yet again the exciting and surprising world of sperm biochemistry, where very...

View article titled, The special systems biology of the sperm

Articles

Involvement of phosphoinositide 3-kinase and Akt in the induction of muscle protein degradation by proteolysis-inducing factor

Steven T. Russell, Helen L. Eley, Stacey M. Wyke, Michael J. Tisdale

Biochem J (2008) 409 (3): 751–759.

https://doi.org/10.1042/BJ20070688

Published: 15 January 2008

... 30 min, which was attenuated by the PI3K (phosphoinositide 3-kinase) inhibitor LY294002 and the tyrosine kinase inhibitor genistein. Protein degradation was attenuated in myotubes expressing a dominant-negative mutant of Akt (termed DNAkt), compared with the wild-type variant, whereas it was enhanced...

View article titled, Involvement of phosphoinositide 3-kinase and Akt in the induction of muscle protein degradation by proteolysis-inducing factor

Articles

Resveratrol is a class IA phosphoinositide 3-kinase inhibitor

Sara Fröjdö, Delphine Cozzone, Hubert Vidal, Luciano Pirola

Biochem J (2007) 406 (3): 511–518.

https://doi.org/10.1042/BJ20070236

Published: 29 August 2007

... indicate that the deacetylase Sir2/SIRT1, an enzyme promoting stress resistance and aging, is the target of resveratrol. Similarly, down-regulation of insulin-like pathways, of which PI3K (phosphoinositide 3-kinase) is a key mediator, promotes longevity and is an attractive strategy to fight cancer. We...

View article titled, Resveratrol is a class IA phosphoinositide 3-kinase inhibitor

Includes: Supplementary data

Articles

The epidermal growth factor receptor (EGFR)-S442F mutant displays increased affinity for neuregulin-2β and agonist-independent coupling with downstream signalling events

Jennifer L. Gilmore, Richard M. Gallo, David J. Riese, II

Biochem J (2006) 396 (1): 79–88.

https://doi.org/10.1042/BJ20051687

Published: 26 April 2006

... mutant than of EGFR-Wt. Furthermore, the affinity of NRG2β for the EGFR-S442F mutant is greater than the affinity of NRG2β for EGFR-Wt. Finally, the EGFR-S442F mutant constitutively suppresses apoptosis via phosphoinositide 3-kinase and Akt signalling but is not highly tyrosine phosphorylated...

View article titled, The epidermal growth factor receptor (EGFR)-S442F mutant displays increased affinity for neuregulin-2β and agonist-independent coupling with downstream signalling events

Articles

Gα q binds to p110α/p85α phosphoinositide 3-kinase and displaces Ras

Lisa M. Ballou, Mohar Chattopadhyay, Yan Li, Suzanne Scarlata, Richard Z. Lin

Biochem J (2006) 394 (3): 557–562.

https://doi.org/10.1042/BJ20051493

Published: 24 February 2006

...Lisa M. Ballou; Mohar Chattopadhyay; Yan Li; Suzanne Scarlata; Richard Z. Lin Several studies have reported that activation of G q -coupled receptors inhibits PI3K (phosphoinositide 3-kinase) signalling. In the present study, we used purified proteins to demonstrate that Gα q directly inhibits...

View article titled, Gα q binds to p110α/p85α phosphoinositide 3-kinase and displaces Ras

Articles

Specific role for p85/p110β in GTP-binding-protein-mediated activation of Akt

Hiroshi Kubo, Kaoru Hazeki, Shunsuke Takasuga, Osamu Hazeki

Biochem J (2005) 392 (3): 607–614.

https://doi.org/10.1042/BJ20050671

Published: 06 December 2005

... activity in the cells in a PI3K- (phosphoinositide 3-kinase) dependent manner. Transfection of p110β into the cells augmented the action of PIA with little effect on insulin. Introduction of a pH1 vector producing shRNA (short hairpin RNA) that targets p110β abolished PIA-induced Akt activation...

View article titled, Specific role for p85/p110β in GTP-binding-protein-mediated activation of Akt

Articles

Muscarinic-receptor-mediated inhibition of insulin-like growth factor-1 receptor-stimulated phosphoinositide 3-kinase signalling in 1321N1 astrocytoma cells

Ian H. BATTY, Ian N. FLEMING, C. Peter DOWNES

Biochem J (2004) 379 (3): 641–651.

https://doi.org/10.1042/bj20031700

Published: 01 May 2004

..., Dundee DD1 5JJ, Scotland, U.K. 6 11 2003 22 1 2004 9 2 2004 9 2 2004 The Biochemical Society, London ©2004 2004 cross-talk feedback regulation of insulin signalling insulin receptor substrate phosphoinositide 3-kinase phospholipase C Abbreviations used...

View article titled, Muscarinic-receptor-mediated inhibition of insulin-like growth factor-1 receptor-stimulated phosphoinositide 3-kinase signalling in 1321N1 astrocytoma cells

Articles

Overexpression of phospholipase D prevents actinomycin D-induced apoptosis through potentiation of phosphoinositide 3-kinase signalling pathways in Chinese-hamster ovary cells

Momoko YAMADA, Yoshiko BANNO, Yoh TAKUWA, Masahiro KODA, Akira HARA, Yoshinori NOZAWA

Biochem J (2004) 378 (2): 649–656.

https://doi.org/10.1042/bj20031398

Published: 01 March 2004

...-survival signalling pathways, such as PI3K (phosphoinositide 3-kinase), Akt, p70S6K (p70 S6 kinase) and ERK (extracellular-signal-regulated kinase). Furthermore, the ActD-induced activation of these survival signalling enzymes was potentiated by overexpression of either PLD1 or PLD2. The PI3K inhibitor...

View article titled, Overexpression of phospholipase D prevents actinomycin D-induced apoptosis through potentiation of phosphoinositide 3-kinase signalling pathways in Chinese-hamster ovary cells

Articles

Transcriptional and translational control of ornithine decarboxylase during Ras transformation

Lisa M. SHANTZ

Biochem J (2004) 377 (1): 257–264.

https://doi.org/10.1042/bj20030778

Published: 01 January 2004

...) activation, whereas activation of the phosphoinositide 3-kinase and the Raf/MEK/ERK pathways were necessary for translational regulation of ODC. The increase in ODC synthesis was accompanied by changes in phosphorylation of eukaryotic initiation factor 4E and its binding protein 4E-BP1. Results show...

View article titled, Transcriptional and translational control of ornithine decarboxylase during Ras transformation

Articles

Mouse system-N amino acid transporter, mNAT3, expressed in hepatocytes and regulated by insulin-activated and phosphoinositide 3-kinase-dependent signalling

Sumin GU, Paul LANGLAIS, Feng LIU, Jean X. JIANG

Biochem J (2003) 371 (3): 721–731.

https://doi.org/10.1042/bj20030049

Published: 01 May 2003

..., and this stimulation was associated closely with an increase in the uptake of l -alanine. Interestingly, this insulin-induced stimulatory effect on mNAT3 expression was attenuated by the phosphoinositide 3-kinase inhibitor LY294002, but not by the mitogen-activated protein kinase inhibitor PD98059, although both...

View article titled, Mouse system-N amino acid transporter, mNAT3, expressed in hepatocytes and regulated by insulin-activated and phosphoinositide 3-kinase-dependent signalling

Articles

Late Simian virus 40 transcription factor is a target of the phosphoinositide 3-kinase/Akt pathway in anti-apoptotic Alzheimers amyloid precursor protein signalling

Tarek KASHOUR, Teralee BURTON, Alexander DIBROV, Francis M. AMARA

Biochem J (2003) 370 (3): 1063–1075.

https://doi.org/10.1042/bj20021197

Published: 15 March 2003

... of dominant-negative forms of either phosphoinositide 3-kinase or Akt. These results demonstrate that LSF activation is required for the neuroprotective effects of APP via phosphoinositide 3-kinase/Akt signalling. Alterations in this pathway by aberrations in APP and/or LSF could promote neuronal loss in AD...

View article titled, Late Simian virus 40 transcription factor is a target of the phosphoinositide 3-kinase/Akt pathway in anti-apoptotic Alzheimers amyloid precursor protein signalling

Articles

Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm

Alexandra C. NEWTON

Biochem J (2003) 370 (2): 361–371.

https://doi.org/10.1042/bj20021626

Published: 01 March 2003

... to its life centre stage as a key signal transducer, and subsequently to its degradation. This review focuses on the life cycle of PKC and the key role played Abbreviations used: PDK-1, phosphoinositide-dependent kinase-1 ; PH, pleckstrin homology; PI 3-kinase, phosphoinositide 3-kinase ; PIF, PDK-1...

View article titled, Regulation of the ABC kinases by phosphorylation: protein kinase C as a paradigm

Articles

Insulin stimulation of pyruvate dehydrogenase in adipocytes involves two distinct signalling pathways

Sam A. JOHNSON, Richard M. DENTON

Biochem J (2003) 369 (2): 351–356.

https://doi.org/10.1042/bj20020920

Published: 15 January 2003

...Sam A. JOHNSON; Richard M. DENTON In isolated rat adipocytes, the insulin stimulation of pyruvate dehydrogenase can be partially inhibited by inhibitors of PI3K (phosphoinositide 3-kinase) and MEK1/2 (mitogen-activated protein kinase/extracellular signal-regulated kinase kinase). In combination...

View article titled, Insulin stimulation of pyruvate dehydrogenase in adipocytes involves two distinct signalling pathways

Articles

α1B-Adrenergic receptor phosphorylation and desensitization induced by transforming growth factor-β

M. Teresa ROMERO-ÁVILA, C. Fabián FLORES-JASSO, J. Adolfo GARCÍA-SÁINZ

Biochem J (2002) 368 (2): 581–587.

https://doi.org/10.1042/bj20021052

Published: 01 December 2002

... thereafter, and concentration-dependent (EC 50 0.3pM). The phosphoinositide 3-kinase inhibitors wortmannin and LY294002, and the protein kinase C inhibitors staurosporine, Ro 318220 and bisindolylmaleimide, blocked the effect of this growth factor. α 1B -Adrenergic receptor phosphorylation was associated...

View article titled, α1B-Adrenergic receptor phosphorylation and desensitization induced by transforming growth factor-β

Articles

Regulation of novel protein kinase C ε by phosphorylation

Vittoria CENNI, Heike DÖPPLER, Erica D. SONNENBURG, Nadir MARALDI, Alexandra C. NEWTON, Alex TOKER

Biochem J (2002) 363 (3): 537–545.

https://doi.org/10.1042/bj3630537

Published: 24 April 2002

... The Biochemical Society, London ©2002 2002 phosphoinositide-dependent kinase 1 phosphoinositide 3-kinase PKC second messengers signal transduction Abbreviations used: A.T.C.C., American Type Culture Collection; BIM I, bisindolylmaleimide I; DAG, diacylglycerol; DMEM, Dulbecco's modified Eagle's...

View article titled, Regulation of novel protein kinase C ε by phosphorylation

Articles

β1 and β2 integrins activate different signalling pathways in monocytes

Merit REYES-REYES, Nancy MORA, Gerardo GONZALEZ, Carlos ROSALES

Biochem J (2002) 363 (2): 273–280.

https://doi.org/10.1042/bj3630273

Published: 08 April 2002

... and phosphoinositide 3-kinase (PI-3K) was then analysed. Nuclear factor κB (NF-κB) activation was also detected in THP-1 cells transiently transfected with an NF-κB-driven luciferase reporter gene. We found that binding of both types of integrin to their natural ligands activated ERK in a Syk- and PI-3K-dependent...

View article titled, β1 and β2 integrins activate different signalling pathways in monocytes

Articles

Platelet-derived growth factor-BB-mediated glycosaminoglycan synthesis is transduced through Akt

Nicholas J. CARTEL, Jinxia WANG, Martin POST

Biochem J (2002) 363 (1): 19–28.

https://doi.org/10.1042/bj3630019

Published: 22 March 2002

...Nicholas J. CARTEL; Jinxia WANG; Martin POST Previously we have demonstrated that the phosphoinositide 3-kinase (PI-3K) signal-transduction pathway mediates platelet-derived growth factor (PDGF)-BB-induced glycosaminoglycan (GAG) synthesis in fetal lung fibroblasts. In the present study we further...

View article titled, Platelet-derived growth factor-BB-mediated glycosaminoglycan synthesis is transduced through Akt

Articles

Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo

Wendy A. KIMBER, Laura TRINKLE-MULCAHY, Peter C. F. CHEUNG, Maria DEAK, Louisa J. MARSDEN, Agnieszka KIELOCH, Stephen WATT, Ronald T. JAVIER, Alex GRAY, C. Peter DOWNES, John M. LUCOCQ, Dario R. ALESSI

Biochem J (2002) 361 (3): 525–536.

https://doi.org/10.1042/bj3610525

Published: 25 January 2002

... or 293 cells with agonists that stimulate PtdIns(3,4) P 2 production results in the marked translocation of TAPP1 to the plasma membrane. This recruitment is dependent on a functional PtdIns(3,4) P 2 -binding PH domain and is inhibited by wortmannin, a phosphoinositide 3-kinase inhibitor that prevents...

View article titled, Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo

Includes: Multimedia, Supplementary data

Articles

Tumour necrosis factor-α activation of protein kinase B in WEHI-164 cells is accompanied by increased phosphorylation of Ser473, but not Thr308

Alison O'TOOLE, S. Kelly MOULE, Peter J. LOCKYER, Andrew P. HALESTRAP

Biochem J (2001) 359 (1): 119–127.

https://doi.org/10.1042/bj3590119

Published: 24 September 2001

... characteristic of the phosphoinositide 3-kinase (PI3-kinase) activation associated with cell survival. Wortmannin also enhanced cell death induced by TNF-α in the presence of actinomycin D, confirming that TNF-α activates a transcription-independent survival pathway requiring PI3-kinase activity. Both TNF-α...

View article titled, Tumour necrosis factor-α activation of protein kinase B in WEHI-164 cells is accompanied by increased phosphorylation of Ser473, but not Thr308

Includes: Supplementary data

Articles

Role for phosphatidylinositol in nuclear envelope formation

Banafshé LARIJANI, Teresa M. BARONA, Dominic L. POCCIA

Biochem J (2001) 356 (2): 495–501.

https://doi.org/10.1042/bj3560495

Published: 24 May 2001

... and in membrane fusion events. Assembly of the nuclear envelope (NE), a crucial step in the progress of mitosis, is also likely to involve membrane fusion reactions. We therefore investigated the role of PtdIns and phosphoinositide 3-kinase (PI-3K) activity in NE formation in vitro . GTP-induced NE formation...

View article titled, Role for phosphatidylinositol in nuclear envelope formation

Articles

Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins

David J. GILLOOLY, Anne SIMONSEN, Harald STENMARK

Biochem J (2001) 355 (2): 249–258.

https://doi.org/10.1042/bj3550249

Published: 06 April 2001

...David J. GILLOOLY; Anne SIMONSEN; Harald STENMARK PtdIns3 P is a phosphoinositide 3-kinase product that has been strongly implicated in regulating membrane trafficking in both mammalian and yeast cells. PtdIns3 P has been shown to be specifically located on membranes associated with the endocytic...

View article titled, Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins

Articles

Ceramide dissociates 3′-phosphoinositide production from pleckstrin homology domain translocation

Suzanne STRATFORD, Daryll B. DEWALD, Scott A. SUMMERS

Biochem J (2001) 354 (2): 359–368.

https://doi.org/10.1042/bj3540359

Published: 22 February 2001

...Suzanne STRATFORD; Daryll B. DEWALD; Scott A. SUMMERS Numerous hormones, cytokines and transforming oncogenes activate phosphoinositide 3-kinase (PI-3K), a lipid kinase that initiates signal transduction cascades regulating cellular proliferation, survival, protein synthesis and glucose metabolism...

View article titled, Ceramide dissociates 3′-phosphoinositide production from pleckstrin homology domain translocation

Articles

Comparison of macrophage responses to oxidized low-density lipoprotein and macrophage colony-stimulating factor (M-CSF or CSF-1)

John A. HAMILTON, Robert BYRNE, Wendy JESSUP, Varuni KANAGASUNDARAM, Genevieve WHITTY

Biochem J (2001) 354 (1): 179–187.

https://doi.org/10.1042/bj3540179

Published: 08 February 2001

... to ox.LDL, as well as their relationship to those mediating CSF-1 action, are unknown. We report here that ox.LDL stimulated extracellular signal-regulated protein kinase (ERK)-1, ERK-2 and phosphoinositide 3-kinase activities in BMM but to a weaker extent than optimal CSF-1 concentrations at the time...

View article titled, Comparison of macrophage responses to oxidized low-density lipoprotein and macrophage colony-stimulating factor (M-CSF or CSF-1)

Articles

Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation

Paula E. ROW, Barbara J. REAVES, Jan DOMIN, J. Paul LUZIO, Howard W. DAVIDSON

Biochem J (2001) 353 (3): 655–661.

https://doi.org/10.1042/bj3530655

Published: 25 January 2001

... 2001 endosome lysosome phosphoinositide 3-kinase trans-Golgi network wortmannin Biochem. J. (2001) 353, 655 661 (Printed in Great Britain) 655 Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation Paula E. ROW*1, Barbara J. REAVES*1,2...

View article titled, Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation

Articles

A role for the actin cytoskeleton in the hormonal and growth-factor-mediated activation of protein kinase B

Karine PEYROLLIER, Eric HAJDUCH, Alexander GRAY, Gary J. LITHERLAND, Alan R. PRESCOTT, Nick R. LESLIE, Harinder S. HUNDAL

Biochem J (2000) 352 (3): 617–622.

https://doi.org/10.1042/bj3520617

Published: 08 December 2000

... growth factor INTRODUCTION The serine}threonine kinase protein kinase B (PKB), which lies downstream of phosphoinositide 3-kinase (PI 3-kinase), plays a key role in regulating diverse cellular functions in response to signalling initiated by activated hormone and growth factor receptors (for review see...

View article titled, A role for the actin cytoskeleton in the hormonal and growth-factor-mediated activation of protein kinase B

Articles

Inhibition of growth-factor-induced phosphorylation and activation of protein kinase B/Akt by atypical protein kinase C in breast cancer cells

Muling MAO, Xianjun FANG, Yiling LU, Ruth LAPUSHIN, Robert C. BAST, JR, Gordon B. MILLS

Biochem J (2000) 352 (2): 475–482.

https://doi.org/10.1042/bj3520475

Published: 24 November 2000

...Muling MAO; Xianjun FANG; Yiling LU; Ruth LAPUSHIN; Robert C. BAST, JR; Gordon B. MILLS The protein kinase B/Akt serine/threonine kinase, located downstream of phosphoinositide 3-kinase (PI-3K), is a major regulator of cellular survival and proliferation. Atypical protein kinase C (aPKC) family...

View article titled, Inhibition of growth-factor-induced phosphorylation and activation of protein kinase B/Akt by atypical protein kinase C in breast cancer cells

Articles

Regulation by glucagon (cAMP) and insulin of the promoter of the human phosphoenolpyruvate carboxykinase gene (cytosolic) in cultured rat hepatocytes and in human hepatoblastoma cells

Anne K. RUCKTÄSCHEL, Daryl K. GRANNER, Bruno CHRIST

Biochem J (2000) 352 (1): 211–217.

https://doi.org/10.1042/bj3520211

Published: 07 November 2000

... by insulin. The phosphoinositide 3-kinase (PI 3-kinase) inhibitor, wortmannin, abolished this action of insulin in cultured rat hepatocytes. The results show that the promoter of the human phosphoenolpyruvate carboxykinase gene mediates the stimulatory action of glucagon and its second messenger cAMP...

View article titled, Regulation by glucagon (cAMP) and insulin of the promoter of the human phosphoenolpyruvate carboxykinase gene (cytosolic) in cultured rat hepatocytes and in human hepatoblastoma cells

Articles

Activation of protein kinase B/cAkt in hepatocytes is sufficient for the induction of expression of the gene encoding glucokinase

Patrick B. IYNEDJIAN, Richard A. ROTH, Mark FLEISCHMANN, Asllan GJINOVCI

Biochem J (2000) 351 (3): 621–627.

https://doi.org/10.1042/bj3510621

Published: 24 October 2000

...-induced increase in glucokinase mRNA seen in unhibited cells, indicating that the phosphoinositide 3-kinase module has a key role. A ligand inducible protein kinase B (PKB, also termed cAkt) fusion protein was expressed by using adenoviral transduction of hepatocytes in primary culture. The PKB activity...

View article titled, Activation of protein kinase B/cAkt in hepatocytes is sufficient for the induction of expression of the gene encoding glucokinase

Articles

Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities

Simon DOWLER, Richard A. CURRIE, David G. CAMPBELL, Maria DEAK, Gursant KULAR, C. Peter DOWNES, Dario R. ALESSI

Biochem J (2000) 351 (1): 19–31.

https://doi.org/10.1042/bj3510019

Published: 26 September 2000

...Simon DOWLER; Richard A. CURRIE; David G. CAMPBELL; Maria DEAK; Gursant KULAR; C. Peter DOWNES; Dario R. ALESSI The second messenger phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5) P 3 ] is generated by the action of phosphoinositide 3-kinase (PI 3-kinase), and regulates a plethora...

View article titled, Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities

Articles

Cross-talk between receptors with intrinsic tyrosine kinase activity and α1b-adrenoceptors

Luz DEL CARMEN MEDINA, José VÁZQUEZ-PRADO, J. Adolfo GARCÍA-SÁINZ

Biochem J (2000) 350 (2): 413–419.

https://doi.org/10.1042/bj3500413

Published: 23 August 2000

... the phosphorylation of these adrenoceptors. The effect of EGF was blocked by tyrphostin AG1478 and that of PDGF was blocked by tyrphostin AG1296, inhibitors of the intrinsic tyrosine kinase activities of the receptors for these growth factors. Wortmannin, an inhibitor of phosphoinositide 3-kinase, blocked the α 1b...

View article titled, Cross-talk between receptors with intrinsic tyrosine kinase activity and α1b-adrenoceptors

Articles

Evidence that 12-lipoxygenase product 12-hydroxyeicosatetraenoic acid activates p21-activated kinase

Yeshao WEN, Jiali GU, Ulla G. KNAUS, Lisa THOMAS, Noe GONZALES, Jerry L. NADLER

Biochem J (2000) 349 (2): 481–487.

https://doi.org/10.1042/bj3490481

Published: 10 July 2000

... of dominant negative Cdc42 and Rac1 inhibited 12-HETE-induced PAK1, strongly suggesting that Cdc42 and Rac1 are the upstream activators of 12-HETE-induced PAK1 activation. Low concentrations (1.5 μ M) of LY294002, a highly specific inhibitor of phosphoinositide 3-kinase (PI-3K), abolished 12-HETE-induced PAK1...

View article titled, Evidence that 12-lipoxygenase product 12-hydroxyeicosatetraenoic acid activates p21-activated kinase

Articles

Action of rolipram on specific PDE4 cAMP phosphodiesterase isoforms and on the phosphorylation of cAMP-response-element-binding protein (CREB) and p38 mitogen-activated protein (MAP) kinase in U937 monocytic cells

Simon J. MACKENZIE, Miles D. HOUSLAY

Biochem J (2000) 347 (2): 571–578.

https://doi.org/10.1042/bj3470571

Published: 10 April 2000

... which was attenuated by both wortmannin and rapamycin. It is proposed that the PDE4A4 isoform is involved in compartmentalized cAMP signalling responses in U937 monocytes. interferon-γ lipopolysaccharide phosphoinositide 3-kinase protein kinase A rapamycin Biochem. J. (2000) 347, 571 578...

View article titled, Action of rolipram on specific PDE4 cAMP phosphodiesterase isoforms and on the phosphorylation of cAMP-response-element-binding protein (CREB) and p38 mitogen-activated protein (MAP) kinase in U937 monocytic cells

Articles

Molecular mechanisms of contraction-regulated cardiac glucose transport

Martin TILL, D. Margriet OUWENS, Alexandra KESSLER, Jürgen ECKEL

Biochem J (2000) 346 (3): 841–847.

https://doi.org/10.1042/bj3460841

Published: 07 March 2000

... rat ventricular cardiomyocytes were electrically stimulated to perform rhythmic contractions to permit the determination of potential sites of convergence of contraction and insulin signalling to glucose transport. The participation of phosphoinositide 3-kinase (PI-3K) in Ca 2+ - and contraction...

View article titled, Molecular mechanisms of contraction-regulated cardiac glucose transport

Articles

Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation

Barbara T. NAVÉ, D. Margriet OUWENS, Dominic J. WITHERS, Dario R. ALESSI, Peter R. SHEPHERD

Biochem J (1999) 344 (2): 427–431.

https://doi.org/10.1042/bj3440427

Published: 24 November 1999

...Barbara T. NAVÉ; D. Margriet OUWENS; Dominic J. WITHERS; Dario R. ALESSI; Peter R. SHEPHERD Growth factor induced activation of phosphoinositide 3-kinase and protein kinase B (PKB) leads to increased activity of the mammalian target of rapamycin (mTOR). This subsequently leads to increased...

View article titled, Mammalian target of rapamycin is a direct target for protein kinase B: identification of a convergence point for opposing effects of insulin and amino-acid deficiency on protein translation

Articles

Mechanisms of inhibition of lipolysis by insulin, vanadate and peroxovanadate in rat adipocytes

Isabelle CASTAN, Jonny WIJKANDER, Vincent MANGANIELLO, Eva DEGERMAN

Biochem J (1999) 339 (2): 281–289.

https://doi.org/10.1042/bj3390281

Published: 08 April 1999

... of the insulin receptor and insulin receptor substrate-1 to a greater extent than insulin, whereas vanadate affected these components little if at all. In addition, only a higher concentration (0.1 mM) of pV induced the tyrosine phosphorylation of p85, the 85 kDa regulatory subunit of phosphoinositide 3-kinase...

View article titled, Mechanisms of inhibition of lipolysis by insulin, vanadate and peroxovanadate in rat adipocytes

Email alerts

CONNECT

EXPLORE

This Feature Is Available To Subscribers Only