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Keywords: phosphoinositide
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Articles
Biochem J (2017) 474 (19): 3307–3319.
Published: 20 September 2017
...Denis Martinez; Béatrice Langlois d'Estaintot; Thierry Granier; James Tolchard; Cécile Courrèges; Valérie Prouzet-Mauléon; Michel Hugues; Bernard Gallois; François Doignon; Benoît Odaert Phosphoinositide lipids recruit proteins to the plasma membrane involved in the regulation of cytoskeleton...
Includes: Supplementary data
Articles
Biochem J (2014) 461 (2): 159–175.
Published: 26 June 2014
...Peter G. Billcliff; Martin Lowe The specific interaction of phosphoinositides with proteins is critical for a plethora of cellular processes, including cytoskeleton remodelling, mitogenic signalling, ion channel regulation and membrane traffic. The spatiotemporal restriction of different...
Articles
Biochem J (2013) 455 (3): 347–358.
Published: 10 October 2013
... collaborate to phosphorylate PIP5K1B at Ser 413 and this reduces its activity and ability to make PtdIns(4,5) P 2 in the cell. energy restriction oxidative stress phosphatidylinositol 4-phosphate 5-kinase phosphoinositide Cells are constantly under attack by damaging agents present...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (3): 413–426.
Published: 12 July 2013
... potent than that of the PPIP5K1 products (IC 50 : Ins P 8 =32 μM and 1-Ins P 7 =43 μM). This rank order of ligand competition with PtdIns(3,4,5) P 3 was also exhibited by the PH (pleckstrin homology) domains of Akt (also known as protein kinase B), GRP1 (general receptor for phosphoinositides 1) and SIN1...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2012) 443 (3): 587–601.
Published: 16 April 2012
...Marco Falasca; Tania Maffucci Class II isoforms of PI3K (phosphoinositide 3-kinase) are still the least investigated and characterized of all PI3Ks. In the last few years, an increased interest in these enzymes has improved our understanding of their cellular functions. However, several questions...
Articles
Biochem J (2012) 441 (1): 399–406.
Published: 14 December 2011
... unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. cancer homologous with E6-associated protein C-terminus (HECT) neural-precursor-cell-expressed developmentally down-regulated 4 (Nedd4) phosphoinositide ubiquitination VPS34...
Articles
Biochem J (2012) 441 (1): 39–59.
Published: 14 December 2011
...Rohan D. Teasdale; Brett M. Collins The mammalian genome encodes 49 proteins that possess a PX (phox-homology) domain, responsible for membrane attachment to organelles of the secretory and endocytic system via binding of phosphoinositide lipids. The PX domain proteins, most of which are classified...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2011) 439 (3): 391–404.
Published: 13 October 2011
...William's Elong Edimo; Rita Derua; Veerle Janssens; Takeshi Nakamura; Jean-Marie Vanderwinden; Etienne Waelkens; Christophe Erneux PtdIns(3,4,5) P 3 and PtdIns(3,4) P 2 are major signalling molecules in mammalian cell biology. PtdIns(3,4) P 2 can be produced by PI3Ks [PI (phosphoinositide) 3...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (3): 597–608.
Published: 13 April 2011
.... Tollip is multimodular, with a conserved C2 domain of unknown function. We found that the Tollip C2 domain preferentially interacts with phosphoinositides, most notably with PtdIns3 P (phosphatidylinositol 3-phosphate) and PtdIns(4,5) P 2 (phosphatidylinositol 4,5-bisphosphate), in a Ca 2+ -independent...
Includes: Supplementary data
Articles
Biochem J (2011) 434 (1): 161–170.
Published: 27 January 2011
...Silke Grunau; Dorothee Lay; Sabrina Mindthoff; Harald W. Platta; Wolfgang Girzalsky; Wilhelm W. Just; Ralf Erdmann PIds (phosphoinositides) are phosphorylated derivatives of the membrane phospholipid PtdIns that have emerged as key regulators of many aspects of cellular physiology. We have...
Articles
Biochem J (2010) 430 (2): 223–235.
Published: 13 August 2010
... 2010 28 6 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 phosphoinositide PtdIns(4,5) P 2 PtdIns5 P PtdIns5 P kinase Combinations of phosphorylation at the 3, 4 or 5 position of the inositol head group of phosphatidylinositol generates seven...
Includes: Supplementary data
Articles
Biochem J (2010) 428 (3): 375–384.
Published: 27 May 2010
... PtdIns5 P using conventional HPLC, owing to poor separation from PtdIns4 P . In the present paper we describe a new HPLC method for resolving PtdIns5 P from PtdIns4 P , which makes possible accurate measurements of basal and inducible levels of cellular PtdIns5 P in the context of other phosphoinositides...
Articles
Biochem J (2010) 425 (1): 159–168.
Published: 14 December 2009
...-terminal polybasic domain of STIM1 with PM phosphoinositides could contribute to STIM1 puncta formation prior to binding to Orai. In the present study, we investigated the role of phosphoinositides in the formation of STIM1 puncta and SOCE (store-operated Ca 2+ entry) in response to store depletion...
Articles
Biochem J (2009) 422 (1): 53–60.
Published: 29 July 2009
...Hrvoje Banfic; Dora Visnjic; Nikica Mise; Sanjeevi Balakrishnan; Simona Deplano; Yuri E. Korchev; Jan Domin Although the class II phosphoinositide 3-kinase enzymes PI3K-C2α and PI3K-C2β act acutely downstream of cell surface receptors they have also been localized to nuclei in mammalian cells...
Articles
Biochem J (2009) 419 (1): 29–49.
Published: 13 March 2009
...Lisa M. Ooms; Kristy A. Horan; Parvin Rahman; Gillian Seaton; Rajendra Gurung; Dharini S. Kethesparan; Christina A. Mitchell Phosphoinositides are membrane-bound signalling molecules that regulate cell proliferation and survival, cytoskeletal reorganization and vesicular trafficking by recruiting...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2009) 419 (1): 1–13.
Published: 13 March 2009
... To whom correspondence should be addressed (email s.k.dove@bham.ac.uk ). 25 9 2008 18 12 2008 22 12 2008 © The Authors Journal compilation © 2009 Biochemical Society 2009 endosome Fab1p/PIKfyve phosphatidyl 3,5-bisphosphate phosphoinositide stress The days when...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2008) 415 (3): e1–e3.
Published: 15 October 2008
...Charles Brearley Phosphoinositides and their binding proteins are regulators of many aspects of the vesicle-trafficking processes that underlie cellular physiology in animal cells. Relatively little is known, by comparison, of the contribution of phosphoinositides to membrane-trafficking phenomena...
Articles
Biochem J (2008) 415 (3): 455–466.
Published: 15 October 2008
...Chao Zhai; Kuoyu Li; Valentini Markaki; John P. Phelan; Katherine Bowers; Frank T. Cooke; Barry Panaretou Phosphoinositide signalling through the eukaryotic plasma membrane makes essential contributions to many processes, including remodelling of the actin cytoskeleton, vesicle trafficking...
Articles
Biochem J (2008) 413 (2): 201–215.
Published: 26 June 2008
... actin dynamics glucose transporter 4 (GLUT4) insulin signalling muscle cells phosphoinositide vesicle traffic Glucose is the major fuel of most mammalian cells, and insulin is its principal regulator. A key function of insulin is to allow glucose entry into muscle and fat cells. Skeletal...
Articles
Biochem J (2008) 411 (2): 441–448.
Published: 27 March 2008
...Shu-Chin Yip; Robert J. Eddy; Angie M. Branch; Huan Pang; Haiyan Wu; Ying Yan; Beth E. Drees; Paul O. Neilsen; John Condeelis; Jonathan M. Backer Class IA PI3Ks (phosphoinositide 3-kinases) generate the secondary messenger PtdIns(3,4,5) P 3 , which plays an important role in many cellular responses...
Articles
Biochem J (2008) 410 (1): 1–17.
Published: 29 January 2008
...Jonathan M. Backer The Class III PI3K (phosphoinositide 3-kinase), Vps34 (vacuolar protein sorting 34), was first described as a component of the vacuolar sorting system in Saccharomyces cerevisiae and is the sole PI3K in yeast. The homologue in mammalian cells, hVps34, has been studied extensively...
Articles
Biochem J (2007) 405 (3): 439–444.
Published: 13 July 2007
... phosphoinositide protein stability PTEN (phosphatase and tensin homologue deleted on chromosome 10) tumour suppressor PTEN (phosphatase and tensin homologue deleted on chromosome 10) regulates many cellular processes, including cell proliferation, survival, growth and motility, principally by inhibiting...
Includes: Supplementary data
Articles
Biochem J (2006) 400 (3): 431–438.
Published: 28 November 2006
... GTP[S] (guanosine 5′-[γ-thio]triphosphate) (τ≈57 min), but not by either ATP or GTP[S] alone. I H also decayed rapidly and was stabilized by the ATP/GTP[S] mixture, but maximal stabilization of I H required further addition of 25 μM PI(3,4)P 2 (phosphoinositide 3,4-bisphosphate) to the cytosolic side...
Articles
Biochem J (2006) 400 (3): 563–572.
Published: 28 November 2006
..., and the PH domain plays a regulatory role that remains poorly understood. We demonstrated previously that Dbl family PH domains bind phosphoinositides with low affinity and cannot function as independent membrane targeting modules. In the present study, we show that dimerization of a Dbs (Dbl's big sister...
Articles
Biochem J (2006) 397 (3): 509–518.
Published: 13 July 2006
... on fluid medium, the mutants lose their viability when deprived of inositol for longer than 24 h. Here, we report a study of the alterations in the first 24 h in cellular inositol, inositol phosphate and phosphoinositide concentrations, whereby a rapidly accumulating phosphorylated compound was detected...
Includes: Supplementary data
Articles
Biochem J (2006) 394 (2): 417–425.
Published: 10 February 2006
...Jianmin Fang; Norma Marchesini; Silvia N. J. Moreno The Toxoplasma gondii phosphoinositide-specific phospholipase C gene ( TgPI - PLC ) was cloned, sequenced and expressed in Escherichia coli and its enzymatic characteristics were investigated. TgPI - PLC is present in the genome as a single-copy...
Articles
Biochem J (2005) 391 (3): e7.
Published: 25 October 2005
... (phosphoinositide-specific phospholipase C) enzymes, PLCη. Two isoforms, PLCη1 and PLCη2, and their splice variants add to the molecular diversity of PLC enzymes. The studies of PLCη regulation suggest that at least some splice variants of PLCη2 could be regulated by the G-protein subunits Gβγ. As two other...
Articles
Biochem J (2005) 391 (2): 231–238.
Published: 10 October 2005
... was visualized by autoradiography. After incubation with liposomes and washing, bound hippocalcin was quantified using scintillation counting. Golgi complex hippocalcin lipid-binding calcium-binding proteins neuronal calcium sensor-1 (NCS-1) phosphoinositide Many cellular proteins...
Articles
Biochem J (2005) 389 (2): 435–441.
Published: 05 July 2005
...Frits M. Flesch; Jong W. Yu; Mark A. Lemmon; Koert N. J. Burger PH-PLCδ 1 [the PH domain (pleckstrin homology domain) of PLCδ 1 (phospholipase C-δ 1 )] is among the best-characterized phosphoinositide-binding domains. PH-PLCδ 1 binds with high specificity to the headgroup of PtdIns(4,5) P 2...
Articles
Biochem J (2005) 389 (1): 207–214.
Published: 21 June 2005
... ). 12 1 2005 14 3 2005 15 3 2005 15 3 2005 The Biochemical Society, London 2005 actin polymerization Dictyostelium endocytosis phospholipase D phosphoinositide PLD (phospholipase D) catalyses the hydrolysis of membrane phospholipids, such as PtdCho...
Includes: Supplementary data
Articles
Biochem J (2005) 385 (1): 65–73.
Published: 14 December 2004
... 2005 calmodulin G-protein phosphoinositide reciprocal regulation regulators of G-protein signalling (RGS) Heterotrimeric G-protein signalling is a major mechanism in cellular signal transduction [ 1 ]. Many elaborate studies have been conducted to examine the main elements...
Articles
Biochem J (2004) 382 (1): 1–11.
Published: 10 August 2004
...Nick R. LESLIE; C. Peter DOWNES The PTEN (phosphatase and tensin homologue deleted on chromosome 10) tumour suppressor is a PI (phosphoinositide) 3-phosphatase that can inhibit cellular proliferation, survival and growth by inactivating PI 3-kinase-dependent signalling. It also suppresses cellular...
Articles
Biochem J (2004) 379 (2): 301–307.
Published: 15 April 2004
...Steven M. WALKER; Nick R. LESLIE; Nevin M. PERERA; Ian H. BATTY; C. Peter DOWNES The PTEN (phosphatase and tensin homologue deleted on chromosome 10) tumour-suppressor protein is a phosphoinositide 3-phosphatase which antagonizes phosphoinositide 3-kinase-dependent signalling by dephosphorylating...
Articles
Biochem J (2004) 378 (3): 1067–1072.
Published: 15 March 2004
...Anne Marie CORGAN; CoreyAyne SINGLETON; Cynthia B. SANTOSO; Jeffrey A. GREENWOOD α-Actinin is a cell-adhesion and cytoskeletal protein that bundles actin microfilaments and links these filaments directly to integrin-adhesion receptors. Phosphoinositides bind to and regulate the interaction of α...
Articles
Biochem J (2004) 377 (3): 653–663.
Published: 01 February 2004
... ©2004 2004 electron microscopy immunogold localization phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4) P 2 ] phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5) P 3 ] phosphoinositide stereology Abbreviations used: COPII, coat protein complex II; DAPP1, dual adaptor...
Articles
Biochem J (2003) 371 (2): 533–540.
Published: 15 April 2003
...Shary N. SHELTON; Barbara BARYLKO; Derk D. BINNS; Bruce F. HORAZDOVSKY; Joseph P. ALBANESI; Joel M. GOODMAN The yeast Saccharomyces cerevisiae contains two known phosphoinositide 4-kinases (PI 4-kinases), which are encoded by PIK1 and STT4 ; both are essential. Pik1p is important for exocytic...
Articles
Biochem J (2002) 366 (3): 689–704.
Published: 15 September 2002
... that underlie phagosomal matu- ration are the topic of this review. Key words: endocytosis, macrophage, neutrophil, phagocytosis, phosphoinositide, Rab, SNARE. non-professional phagocytes , paraprofessional phagocytes and professional phagocytes , referring to cells with low, average and high phagocytic...
Articles
Biochem J (2002) 363 (3): 657–666.
Published: 24 April 2002
... 10 2001 24 1 2002 28 2 2002 The Biochemical Society, London ©2002 2002 immunoelectron microscopy immunogold lipid domains localization phosphoinositide Abbreviations used: PH, pleckstrin homology; GST, glutathione S-transferase; GFP, green fluorescent protein; PLCδ...
Articles
Biochem J (2002) 361 (3): 525–536.
Published: 25 January 2002
... (PH)-domain-containing proteins, termed ‘ ta ndem- P H-domain-containing p rotein- 1 ’ (TAPP1) and TAPP2, which interacted in vitro with high affinity with PtdIns(3,4) P 2 , but did not bind PtdIns(3,4,5) P 3 or other phosphoinositides. In the present study we demonstrate that stimulation of Swiss 3T3...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2001) 359 (3): 583–589.
Published: 25 October 2001
... A. thaliana . Phosphorylation of AtPIP5K1 by protein kinase A is accompanied by a 40% inhibition of its catalytic activity. Full activity is recovered by treating phosphorylated AtPIP5K1 with alkaline phosphatase. lipid kinase phosphoinositide plant regulation Abbreviations used: GST...
Articles
Biochem J (2001) 359 (1): 165–173.
Published: 24 September 2001
...Rikke B. JENSEN; Tanja LACOUR; Jakob ALBRETHSEN; Michael NIELSEN; Karen SKRIVER Classic FYVE zinc-finger domains recognize the phosphoinositide signal PtdIns3 P and share the basic (R/K) 1 (R/K)HHCR 6 (single-letter amino acid codes) consensus sequence. This domain is present in predicted PtdIns3 P...
Articles
Biochem J (2001) 357 (2): 427–435.
Published: 09 July 2001
...Nick R. LESLIE; Deborah BENNETT; Alex GRAY; Ian PASS; Khe HOANG-XUAN; C. Peter DOWNES The tumour suppressor protein PTEN (phosphatase and tensin homolog deleted on chromosome 10) is a lipid phosphatase which can antagonize the phosphoinositide 3-kinase (PI 3-kinase) signalling pathway, promoting...
Articles
Biochem J (2001) 355 (2): 249–258.
Published: 06 April 2001
...David J. GILLOOLY; Anne SIMONSEN; Harald STENMARK PtdIns3 P is a phosphoinositide 3-kinase product that has been strongly implicated in regulating membrane trafficking in both mammalian and yeast cells. PtdIns3 P has been shown to be specifically located on membranes associated with the endocytic...
Articles
Biochem J (2000) 350 (2): 337–352.
Published: 23 August 2000
... the mammalian phosphoinositide 5-phosphatase synaptojanin and the yeast synaptojanin homologues Inp51p, Inp52p and Inp53p. These proteins therefore contain both Sac phosphatase and 5-phosphatase domains. This review describes the Sac phosphatase domain-containing proteins and their actions, with particular...
Articles
Biochem J (2000) 350 (1): 1–18.
Published: 09 August 2000
..., their only clearly demonstrated physiological function to date is to bind membrane phosphoinositides. The PH domain from phospholipase C-δ 1 binds specifically to PtdIns(4,5) P 2 and its headgroup, and has become a valuable tool for studying cellular PtdIns(4,5) P 2 functions. More recent developments have...
Articles
Biochem J (1999) 342 (1): 7–12.
Published: 10 August 1999
... phosphorylated proteins and 3-phosphoinositides and may therefore play a role in regulating the location and/or activity of such proteins(s) in response to agonists that elevate PtdIns(3,4,5) P 3 and PtdIns(3,4) P 2 . Biochem. J. (1999) 342, 7 12 (Printed in Great Britain) 7 DAPP1 : a dual adaptor...