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Articles

Structural evidence of a phosphoinositide-binding site in the Rgd1-RhoGAP domain

Denis Martinez, Béatrice Langlois d'Estaintot, Thierry Granier, James Tolchard, Cécile Courrèges, Valérie Prouzet-Mauléon, Michel Hugues, Bernard Gallois, François Doignon, Benoît Odaert

Biochem J (2017) 474 (19): 3307–3319.

https://doi.org/10.1042/BCJ20170331

Published: 20 September 2017

...Denis Martinez; Béatrice Langlois d'Estaintot; Thierry Granier; James Tolchard; Cécile Courrèges; Valérie Prouzet-Mauléon; Michel Hugues; Bernard Gallois; François Doignon; Benoît Odaert Phosphoinositide lipids recruit proteins to the plasma membrane involved in the regulation of cytoskeleton...

View article titled, Structural evidence of a phosphoinositide-binding site in the Rgd1-RhoGAP domain

Includes: Supplementary data

Articles

Inositol lipid phosphatases in membrane trafficking and human disease

Peter G. Billcliff, Martin Lowe

Biochem J (2014) 461 (2): 159–175.

https://doi.org/10.1042/BJ20140361

Published: 26 June 2014

...Peter G. Billcliff; Martin Lowe The specific interaction of phosphoinositides with proteins is critical for a plethora of cellular processes, including cytoskeleton remodelling, mitogenic signalling, ion channel regulation and membrane traffic. The spatiotemporal restriction of different...

View article titled, Inositol lipid phosphatases in membrane trafficking and human disease

Articles

Collaboration of AMPK and PKC to induce phosphorylation of Ser 413 on PIP5K1B resulting in decreased kinase activity and reduced PtdIns(4,5) P 2 synthesis in response to oxidative stress and energy restriction

Iman van den Bout, David R. Jones, Zahid H. Shah, Jonathan R. Halstead, Willem-Jan Keune, Shabaz Mohammed, Clive S. D’Santos, Nullin Divecha

Biochem J (2013) 455 (3): 347–358.

https://doi.org/10.1042/BJ20130259

Published: 10 October 2013

... transfected using FuGENE® 6 (Roche). HEK-293 cells were transfected using the calcium phosphate method. energy restriction oxidative stress phosphatidylinositol 4-phosphate 5-kinase phosphoinositide Cells are constantly under attack by damaging agents present in the extracellular matrix...

View article titled, Collaboration of AMPK and PKC to induce phosphorylation of Ser 413 on PIP5K1B resulting in decreased kinase activity and reduced PtdIns(4,5) P 2 synthesis in response to oxidative stress and energy restriction

Includes: Supplementary data

Articles

PPIP5K1 modulates ligand competition between diphosphoinositol polyphosphates and PtdIns(3,4,5) P 3 for polyphosphoinositide-binding domains

Nikhil A. Gokhale, Angelika Zaremba, Agnes K. Janoshazi, Jeremy D. Weaver, Stephen B. Shears

Biochem J (2013) 453 (3): 413–426.

https://doi.org/10.1042/BJ20121528

Published: 12 July 2013

... potent than that of the PPIP5K1 products (IC 50 : Ins P 8 =32 μM and 1-Ins P 7 =43 μM). This rank order of ligand competition with PtdIns(3,4,5) P 3 was also exhibited by the PH (pleckstrin homology) domains of Akt (also known as protein kinase B), GRP1 (general receptor for phosphoinositides 1) and SIN1...

View article titled, PPIP5K1 modulates ligand competition between diphosphoinositol polyphosphates and PtdIns(3,4,5) P 3 for polyphosphoinositide-binding domains

Includes: Multimedia, Supplementary data

Articles

Regulation and cellular functions of class II phosphoinositide 3-kinases

Marco Falasca, Tania Maffucci

Biochem J (2012) 443 (3): 587–601.

https://doi.org/10.1042/BJ20120008

Published: 16 April 2012

...Marco Falasca; Tania Maffucci Class II isoforms of PI3K (phosphoinositide 3-kinase) are still the least investigated and characterized of all PI3Ks. In the last few years, an increased interest in these enzymes has improved our understanding of their cellular functions. However, several questions...

View article titled, Regulation and cellular functions of class II phosphoinositide 3-kinases

Articles

Nedd4-dependent lysine-11-linked polyubiquitination of the tumour suppressor Beclin 1

Harald W. Platta, Hilde Abrahamsen, Sigrid B. Thoresen, Harald Stenmark

Biochem J (2012) 441 (1): 399–406.

https://doi.org/10.1042/BJ20111424

Published: 14 December 2011

... subjected to immunoprecipitation. 1 To whom correspondence should be addressed (email stenmark@ulrik.uio.no ). Phosphoinositides, phosphorylated derivatives of PtdIns, control cellular functions through recruitment of cytosolic proteins to specific membranes. The PI3K-III (class III PI 3...

View article titled, Nedd4-dependent lysine-11-linked polyubiquitination of the tumour suppressor Beclin 1

Articles

Insights into the PX (phox-homology) domain and SNX (sorting nexin) protein families: structures, functions and roles in disease

Rohan D. Teasdale, Brett M. Collins

Biochem J (2012) 441 (1): 39–59.

https://doi.org/10.1042/BJ20111226

Published: 14 December 2011

...Rohan D. Teasdale; Brett M. Collins The mammalian genome encodes 49 proteins that possess a PX (phox-homology) domain, responsible for membrane attachment to organelles of the secretory and endocytic system via binding of phosphoinositide lipids. The PX domain proteins, most of which are classified...

View article titled, Insights into the PX (phox-homology) domain and SNX (sorting nexin) protein families: structures, functions and roles in disease

Includes: Multimedia, Supplementary data

Articles

Evidence of SHIP2 Ser 132 phosphorylation, its nuclear localization and stability

William's Elong Edimo, Rita Derua, Veerle Janssens, Takeshi Nakamura, Jean-Marie Vanderwinden, Etienne Waelkens, Christophe Erneux

Biochem J (2011) 439 (3): 391–404.

https://doi.org/10.1042/BJ20110173

Published: 13 October 2011

...William's Elong Edimo; Rita Derua; Veerle Janssens; Takeshi Nakamura; Jean-Marie Vanderwinden; Etienne Waelkens; Christophe Erneux PtdIns(3,4,5) P 3 and PtdIns(3,4) P 2 are major signalling molecules in mammalian cell biology. PtdIns(3,4) P 2 can be produced by PI3Ks [PI (phosphoinositide) 3...

View article titled, Evidence of SHIP2 Ser 132 phosphorylation, its nuclear localization and stability

Includes: Supplementary data

Articles

The C2 domain of Tollip, a Toll-like receptor signalling regulator, exhibits broad preference for phosphoinositides

Gayatri Ankem, Sharmistha Mitra, Furong Sun, Anna C. Moreno, Boonta Chutvirasakul, Hugo F. Azurmendi, Liwu Li, Daniel G. S. Capelluto

Biochem J (2011) 435 (3): 597–608.

https://doi.org/10.1042/BJ20102160

Published: 13 April 2011

.... Tollip is multimodular, with a conserved C2 domain of unknown function. We found that the Tollip C2 domain preferentially interacts with phosphoinositides, most notably with PtdIns3 P (phosphatidylinositol 3-phosphate) and PtdIns(4,5) P 2 (phosphatidylinositol 4,5-bisphosphate), in a Ca 2+ -independent...

View article titled, The C2 domain of Tollip, a Toll-like receptor signalling regulator, exhibits broad preference for phosphoinositides

Includes: Supplementary data

Articles

The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae

Silke Grunau, Dorothee Lay, Sabrina Mindthoff, Harald W. Platta, Wolfgang Girzalsky, Wilhelm W. Just, Ralf Erdmann

Biochem J (2011) 434 (1): 161–170.

https://doi.org/10.1042/BJ20101115

Published: 27 January 2011

...Silke Grunau; Dorothee Lay; Sabrina Mindthoff; Harald W. Platta; Wolfgang Girzalsky; Wilhelm W. Just; Ralf Erdmann PIds (phosphoinositides) are phosphorylated derivatives of the membrane phospholipid PtdIns that have emerged as key regulators of many aspects of cellular physiology. We have...

View article titled, The phosphoinositide 3-kinase Vps34p is required for pexophagy in Saccharomyces cerevisiae

Articles

PIP4Kβ interacts with and modulates nuclear localization of the high-activity PtdIns5 P -4-kinase isoform PIP4Kα

Yvette Bultsma, Willem-Jan Keune, Nullin Divecha

Biochem J (2010) 430 (2): 223–235.

https://doi.org/10.1042/BJ20100341

Published: 13 August 2010

... be important in their physiological and pathological roles. 2 To whom correspondence should be addressed (email ndivecha@picr.man.ac.uk ). 8 3 2010 7 6 2010 28 6 2010 28 6 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 phosphoinositide PtdIns(4,5...

View article titled, PIP4Kβ interacts with and modulates nuclear localization of the high-activity PtdIns5 P -4-kinase isoform PIP4Kα

Includes: Supplementary data

Articles

A novel HPLC-based approach makes possible the spatial characterization of cellular PtdIns5 P and other phosphoinositides

Deborah Sarkes, Lucia E. Rameh

Biochem J (2010) 428 (3): 375–384.

https://doi.org/10.1042/BJ20100129

Published: 27 May 2010

... PtdIns5 P using conventional HPLC, owing to poor separation from PtdIns4 P . In the present paper we describe a new HPLC method for resolving PtdIns5 P from PtdIns4 P , which makes possible accurate measurements of basal and inducible levels of cellular PtdIns5 P in the context of other phosphoinositides...

View article titled, A novel HPLC-based approach makes possible the spatial characterization of cellular PtdIns5 P and other phosphoinositides

Articles

Role of phosphoinositides in STIM1 dynamics and store-operated calcium entry

Ciara M. Walsh, Michael Chvanov, Lee P. Haynes, Ole H. Petersen, Alexei V. Tepikin, Robert D. Burgoyne

Biochem J (2010) 425 (1): 159–168.

https://doi.org/10.1042/BJ20090884

Published: 14 December 2009

...-terminal polybasic domain of STIM1 with PM phosphoinositides could contribute to STIM1 puncta formation prior to binding to Orai. In the present study, we investigated the role of phosphoinositides in the formation of STIM1 puncta and SOCE (store-operated Ca 2+ entry) in response to store depletion...

View article titled, Role of phosphoinositides in STIM1 dynamics and store-operated calcium entry

Articles

Epidermal growth factor stimulates translocation of the class II phosphoinositide 3-kinase PI3K-C2β to the nucleus

Hrvoje Banfic, Dora Visnjic, Nikica Mise, Sanjeevi Balakrishnan, Simona Deplano, Yuri E. Korchev, Jan Domin

Biochem J (2009) 422 (1): 53–60.

https://doi.org/10.1042/BJ20090654

Published: 29 July 2009

...Hrvoje Banfic; Dora Visnjic; Nikica Mise; Sanjeevi Balakrishnan; Simona Deplano; Yuri E. Korchev; Jan Domin Although the class II phosphoinositide 3-kinase enzymes PI3K-C2α and PI3K-C2β act acutely downstream of cell surface receptors they have also been localized to nuclei in mammalian cells...

View article titled, Epidermal growth factor stimulates translocation of the class II phosphoinositide 3-kinase PI3K-C2β to the nucleus

Articles

The role of the inositol polyphosphate 5-phosphatases in cellular function and human disease

Lisa M. Ooms, Kristy A. Horan, Parvin Rahman, Gillian Seaton, Rajendra Gurung, Dharini S. Kethesparan, Christina A. Mitchell

Biochem J (2009) 419 (1): 29–49.

https://doi.org/10.1042/BJ20081673

Published: 13 March 2009

...Lisa M. Ooms; Kristy A. Horan; Parvin Rahman; Gillian Seaton; Rajendra Gurung; Dharini S. Kethesparan; Christina A. Mitchell Phosphoinositides are membrane-bound signalling molecules that regulate cell proliferation and survival, cytoskeletal reorganization and vesicular trafficking by recruiting...

View article titled, The role of the inositol polyphosphate 5-phosphatases in cellular function and human disease

Includes: Multimedia, Supplementary data

Articles

Phosphatidylinositol 3,5-bisphosphate and Fab1p/PIKfyve underPPIn endo-lysosome function

Stephen K. Dove, Kangzhen Dong, Takafumi Kobayashi, Fay K. Williams, Robert H. Michell

Biochem J (2009) 419 (1): 1–13.

https://doi.org/10.1042/BJ20081950

Published: 13 March 2009

... should be addressed (email s.k.dove@bham.ac.uk ). 25 9 2008 18 12 2008 22 12 2008 © The Authors Journal compilation © 2009 Biochemical Society 2009 endosome Fab1p/PIKfyve phosphatidyl 3,5-bisphosphate phosphoinositide stress The existence of PtdIns(3,5) P 2...

View article titled, Phosphatidylinositol 3,5-bisphosphate and Fab1p/PIKfyve underPPIn endo-lysosome function

Includes: Multimedia, Supplementary data

Articles

Sorting out PtdIns(4,5) P 2 and clathrin-coated vesicles in plants

Charles Brearley

Biochem J (2008) 415 (3): e1–e3.

https://doi.org/10.1042/BJ20081830

Published: 15 October 2008

...Charles Brearley Phosphoinositides and their binding proteins are regulators of many aspects of the vesicle-trafficking processes that underlie cellular physiology in animal cells. Relatively little is known, by comparison, of the contribution of phosphoinositides to membrane-trafficking phenomena...

View article titled, Sorting out PtdIns(4,5) P 2 and clathrin-coated vesicles in plants

Articles

Ypp1/YGR198w plays an essential role in phosphoinositide signalling at the plasma membrane

Chao Zhai, Kuoyu Li, Valentini Markaki, John P. Phelan, Katherine Bowers, Frank T. Cooke, Barry Panaretou

Biochem J (2008) 415 (3): 455–466.

https://doi.org/10.1042/BJ20080209

Published: 15 October 2008

...Chao Zhai; Kuoyu Li; Valentini Markaki; John P. Phelan; Katherine Bowers; Frank T. Cooke; Barry Panaretou Phosphoinositide signalling through the eukaryotic plasma membrane makes essential contributions to many processes, including remodelling of the actin cytoskeleton, vesicle trafficking...

View article titled, Ypp1/YGR198w plays an essential role in phosphoinositide signalling at the plasma membrane

Articles

Insulin action on glucose transporters through molecular switches, tracks and tethers

Hilal Zaid, Costin N. Antonescu, Varinder K. Randhawa, Amira Klip

Biochem J (2008) 413 (2): 201–215.

https://doi.org/10.1042/BJ20080723

Published: 26 June 2008

... transporter 4 (GLUT4) insulin signalling muscle cells phosphoinositide vesicle traffic Glucose is the major fuel of most mammalian cells, and insulin is its principal regulator. A key function of insulin is to allow glucose entry into muscle and fat cells. Skeletal muscle is the largest site...

View article titled, Insulin action on glucose transporters through molecular switches, tracks and tethers

Articles

Quantification of PtdIns(3,4,5) P 3 dynamics in EGF-stimulated carcinoma cells: a comparison of PH-domain-mediated methods with immunological methods

Shu-Chin Yip, Robert J. Eddy, Angie M. Branch, Huan Pang, Haiyan Wu, Ying Yan, Beth E. Drees, Paul O. Neilsen, John Condeelis, Jonathan M. Backer

Biochem J (2008) 411 (2): 441–448.

https://doi.org/10.1042/BJ20071179

Published: 27 March 2008

...Shu-Chin Yip; Robert J. Eddy; Angie M. Branch; Huan Pang; Haiyan Wu; Ying Yan; Beth E. Drees; Paul O. Neilsen; John Condeelis; Jonathan M. Backer Class IA PI3Ks (phosphoinositide 3-kinases) generate the secondary messenger PtdIns(3,4,5) P 3 , which plays an important role in many cellular responses...

View article titled, Quantification of PtdIns(3,4,5) P 3 dynamics in EGF-stimulated carcinoma cells: a comparison of PH-domain-mediated methods with immunological methods

Articles

The regulation and function of Class III PI3Ks: novel roles for Vps34

Jonathan M. Backer

Biochem J (2008) 410 (1): 1–17.

https://doi.org/10.1042/BJ20071427

Published: 29 January 2008

...Jonathan M. Backer The Class III PI3K (phosphoinositide 3-kinase), Vps34 (vacuolar protein sorting 34), was first described as a component of the vacuolar sorting system in Saccharomyces cerevisiae and is the sole PI3K in yeast. The homologue in mammalian cells, hVps34, has been studied extensively...

View article titled, The regulation and function of Class III PI3Ks: novel roles for Vps34

Articles

PTEN is destabilized by phosphorylation on Thr 366

Helene Maccario, Nevin M. Perera, Lindsay Davidson, C. Peter Downes, Nick R. Leslie

Biochem J (2007) 405 (3): 439–444.

https://doi.org/10.1042/BJ20061837

Published: 13 July 2007

... phosphoinositide protein stability PTEN (phosphatase and tensin homologue deleted on chromosome 10) tumour suppressor PTEN (phosphatase and tensin homologue deleted on chromosome 10) regulates many cellular processes, including cell proliferation, survival, growth and motility, principally by inhibiting...

View article titled, PTEN is destabilized by phosphorylation on Thr 366

Includes: Supplementary data

Articles

Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase

Gábor L. Petheő, Nathalie C. Girardin, Nicolas Goossens, Gergely Z. Molnár, Nicolas Demaurex

Biochem J (2006) 400 (3): 431–438.

https://doi.org/10.1042/BJ20060578

Published: 28 November 2006

... GTP[S] (guanosine 5′-[γ-thio]triphosphate) (τ≈57 min), but not by either ATP or GTP[S] alone. I H also decayed rapidly and was stabilized by the ATP/GTP[S] mixture, but maximal stabilization of I H required further addition of 25 μM PI(3,4)P 2 (phosphoinositide 3,4-bisphosphate) to the cytosolic side...

View article titled, Role of nucleotides and phosphoinositides in the stability of electron and proton currents associated with the phagocytic NADPH oxidase

Articles

The Dbs PH domain contributes independently to membrane targeting and regulation of guanine nucleotide-exchange activity

Mark A. Baumeister, Kent L. Rossman, John Sondek, Mark A. Lemmon

Biochem J (2006) 400 (3): 563–572.

https://doi.org/10.1042/BJ20061020

Published: 28 November 2006

..., and the PH domain plays a regulatory role that remains poorly understood. We demonstrated previously that Dbl family PH domains bind phosphoinositides with low affinity and cannot function as independent membrane targeting modules. In the present study, we show that dimerization of a Dbs (Dbl's big sister...

View article titled, The Dbs PH domain contributes independently to membrane targeting and regulation of guanine nucleotide-exchange activity

Articles

Disruption of inositol biosynthesis through targeted mutagenesis in Dictyostelium discoideum : generation and characterization of inositol-auxotrophic mutants

Andreas Fischbach, Stephan Adelt, Alexander Müller, Günter Vogel

Biochem J (2006) 397 (3): 509–518.

https://doi.org/10.1042/BJ20060277

Published: 13 July 2006

... on fluid medium, the mutants lose their viability when deprived of inositol for longer than 24 h. Here, we report a study of the alterations in the first 24 h in cellular inositol, inositol phosphate and phosphoinositide concentrations, whereby a rapidly accumulating phosphorylated compound was detected...

View article titled, Disruption of inositol biosynthesis through targeted mutagenesis in Dictyostelium discoideum : generation and characterization of inositol-auxotrophic mutants

Includes: Supplementary data

Articles

A Toxoplasma gondii phosphoinositide phospholipase C ( Tg PI-PLC) with high affinity for phosphatidylinositol

Jianmin Fang, Norma Marchesini, Silvia N. J. Moreno

Biochem J (2006) 394 (2): 417–425.

https://doi.org/10.1042/BJ20051393

Published: 10 February 2006

...Jianmin Fang; Norma Marchesini; Silvia N. J. Moreno The Toxoplasma gondii phosphoinositide-specific phospholipase C gene ( TgPI - PLC ) was cloned, sequenced and expressed in Escherichia coli and its enzymatic characteristics were investigated. TgPI - PLC is present in the genome as a single-copy...

View article titled, A Toxoplasma gondii phosphoinositide phospholipase C ( Tg PI-PLC) with high affinity for phosphatidylinositol

Articles

New insights into the families of PLC enzymes: looking back and going forward

Matilda Katan

Biochem J (2005) 391 (3): e7.

https://doi.org/10.1042/BJ20051506

Published: 25 October 2005

... (phosphoinositide-specific phospholipase C) enzymes, PLCη. Two isoforms, PLCη1 and PLCη2, and their splice variants add to the molecular diversity of PLC enzymes. The studies of PLCη regulation suggest that at least some splice variants of PLCη2 could be regulated by the G-protein subunits Gβγ. As two other...

View article titled, New insights into the families of PLC enzymes: looking back and going forward

Articles

High-affinity interaction of the N-terminal myristoylation motif of the neuronal calcium sensor protein hippocalcin with phosphatidylinositol 4,5-bisphosphate

Dermott W. O'Callaghan, Lee P. Haynes, Robert D. Burgoyne

Biochem J (2005) 391 (2): 231–238.

https://doi.org/10.1042/BJ20051001

Published: 10 October 2005

.... Carlton J. McGregor A. H. Gleeson P. A. Teasdale R. D. Mellor H. Cullen P. J. The phox homology (PX) domain-dependent, 3-phosphoinositide-mediated association of sorting nexin-1 with an early sorting endosomal compartment is required for its ability to regulate epidermal growth...

View article titled, High-affinity interaction of the N-terminal myristoylation motif of the neuronal calcium sensor protein hippocalcin with phosphatidylinositol 4,5-bisphosphate

Articles

Membrane activity of the phospholipase C-δ 1 pleckstrin homology (PH) domain

Frits M. Flesch, Jong W. Yu, Mark A. Lemmon, Koert N. J. Burger

Biochem J (2005) 389 (2): 435–441.

https://doi.org/10.1042/BJ20041721

Published: 05 July 2005

...Frits M. Flesch; Jong W. Yu; Mark A. Lemmon; Koert N. J. Burger PH-PLCδ 1 [the PH domain (pleckstrin homology domain) of PLCδ 1 (phospholipase C-δ 1 )] is among the best-characterized phosphoinositide-binding domains. PH-PLCδ 1 binds with high specificity to the headgroup of PtdIns(4,5) P 2...

View article titled, Membrane activity of the phospholipase C-δ 1 pleckstrin homology (PH) domain

Articles

Phospholipase D activity is essential for actin localization and actin-based motility in Dictyostelium

Soha ZOUWAIL, Trevor R. PETTITT, Stephen K. DOVE, Margarita V. CHIBALINA, Dale J. POWNER, Lee HAYNES, Michael J. O. WAKELAM, Robert H. INSALL

Biochem J (2005) 389 (1): 207–214.

https://doi.org/10.1042/BJ20050085

Published: 21 June 2005

..., but at aberrant locations within the cell. We show further that this response is mediated via a phosphoinositide-4-phosphate 5-kinase activity, implying a role for PLD-regulated synthesis of PtdIns(4,5) P 2 in normal Dictyostelium motility. Dictyostelium cells were grown axenically in HL-5...

View article titled, Phospholipase D activity is essential for actin localization and actin-based motility in Dictyostelium

Includes: Supplementary data

Articles

Phosphatidylinositol 3,4,5-trisphosphate and Ca 2+ /calmodulin competitively bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action

Masaru ISHII, Satoru FUJITA, Mitsuhiko YAMADA, Yukio HOSAKA, Yoshihisa KURACHI

Biochem J (2005) 385 (1): 65–73.

https://doi.org/10.1042/BJ20040404

Published: 14 December 2004

... 2005 calmodulin G-protein phosphoinositide reciprocal regulation regulators of G-protein signalling (RGS) Heterotrimeric G-protein signalling is a major mechanism in cellular signal transduction [ 1 ]. Many elaborate studies have been conducted to examine the main elements...

View article titled, Phosphatidylinositol 3,4,5-trisphosphate and Ca 2+ /calmodulin competitively bind to the regulators of G-protein-signalling (RGS) domain of RGS4 and reciprocally regulate its action

Articles

PTEN function: how normal cells control it and tumour cells lose it

Nick R. LESLIE, C. Peter DOWNES

Biochem J (2004) 382 (1): 1–11.

https://doi.org/10.1042/BJ20040825

Published: 10 August 2004

...Nick R. LESLIE; C. Peter DOWNES The PTEN (phosphatase and tensin homologue deleted on chromosome 10) tumour suppressor is a PI (phosphoinositide) 3-phosphatase that can inhibit cellular proliferation, survival and growth by inactivating PI 3-kinase-dependent signalling. It also suppresses cellular...

View article titled, PTEN function: how normal cells control it and tumour cells lose it

Articles

The tumour-suppressor function of PTEN requires an N-terminal lipid-binding motif

Steven M. WALKER, Nick R. LESLIE, Nevin M. PERERA, Ian H. BATTY, C. Peter DOWNES

Biochem J (2004) 379 (2): 301–307.

https://doi.org/10.1042/bj20031839

Published: 15 April 2004

...Steven M. WALKER; Nick R. LESLIE; Nevin M. PERERA; Ian H. BATTY; C. Peter DOWNES The PTEN (phosphatase and tensin homologue deleted on chromosome 10) tumour-suppressor protein is a phosphoinositide 3-phosphatase which antagonizes phosphoinositide 3-kinase-dependent signalling by dephosphorylating...

View article titled, The tumour-suppressor function of PTEN requires an N-terminal lipid-binding motif

Articles

Phosphoinositides differentially regulate alpha-actinin flexibility and function

Anne Marie CORGAN, CoreyAyne SINGLETON, Cynthia B. SANTOSO, Jeffrey A. GREENWOOD

Biochem J (2004) 378 (3): 1067–1072.

https://doi.org/10.1042/bj20031124

Published: 15 March 2004

...Anne Marie CORGAN; CoreyAyne SINGLETON; Cynthia B. SANTOSO; Jeffrey A. GREENWOOD α-Actinin is a cell-adhesion and cytoskeletal protein that bundles actin microfilaments and links these filaments directly to integrin-adhesion receptors. Phosphoinositides bind to and regulate the interaction of α...

View article titled, Phosphoinositides differentially regulate alpha-actinin flexibility and function

Articles

Detection of novel intracellular agonist responsive pools of phosphatidylinositol 3,4-bisphosphate using the TAPP1 pleckstrin homology domain in immunoelectron microscopy

Stephen A. WATT, Wendy A. KIMBER, Ian N. FLEMING, Nick R. LESLIE, C. Peter DOWNES, John M. LUCOCQ

Biochem J (2004) 377 (3): 653–663.

https://doi.org/10.1042/bj20031397

Published: 01 February 2004

... ©2004 2004 electron microscopy immunogold localization phosphatidylinositol 3,4-bisphosphate [PtdIns(3,4) P 2 ] phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5) P 3 ] phosphoinositide stereology Abbreviations used: COPII, coat protein complex II; DAPP1, dual adaptor...

View article titled, Detection of novel intracellular agonist responsive pools of phosphatidylinositol 3,4-bisphosphate using the TAPP1 pleckstrin homology domain in immunoelectron microscopy

Articles

Saccharomyces cerevisiae contains a Type II phosphoinositide 4-kinase

Shary N. SHELTON, Barbara BARYLKO, Derk D. BINNS, Bruce F. HORAZDOVSKY, Joseph P. ALBANESI, Joel M. GOODMAN

Biochem J (2003) 371 (2): 533–540.

https://doi.org/10.1042/bj20021407

Published: 15 April 2003

...Shary N. SHELTON; Barbara BARYLKO; Derk D. BINNS; Bruce F. HORAZDOVSKY; Joseph P. ALBANESI; Joel M. GOODMAN The yeast Saccharomyces cerevisiae contains two known phosphoinositide 4-kinases (PI 4-kinases), which are encoded by PIK1 and STT4 ; both are essential. Pik1p is important for exocytic...

View article titled, Saccharomyces cerevisiae contains a Type II phosphoinositide 4-kinase

Articles

Phagosome maturation: aging gracefully

Otilia V. VIEIRA, Roberto J. BOTELHO, Sergio GRINSTEIN

Biochem J (2002) 366 (3): 689–704.

https://doi.org/10.1042/bj20020691

Published: 15 September 2002

... that underlie phagosomal matu- ration are the topic of this review. Key words: endocytosis, macrophage, neutrophil, phagocytosis, phosphoinositide, Rab, SNARE. non-professional phagocytes , paraprofessional phagocytes and professional phagocytes , referring to cells with low, average and high phagocytic...

View article titled, Phagosome maturation: aging gracefully

Articles

Subcellular localization of phosphatidylinositol 4,5-bisphosphate using the pleckstrin homology domain of phospholipase C δ1

Stephen A. WATT, Gursant KULAR, Ian N. FLEMING, C. Peter DOWNES, John M. LUCOCQ

Biochem J (2002) 363 (3): 657–666.

https://doi.org/10.1042/bj3630657

Published: 24 April 2002

.... Thus we have developed a highly selective method for mapping the PtdIns(4,5) P 2 distribution within cells at high resolution, and our data provide direct evidence for this lipid at key functional locations. immunoelectron microscopy immunogold lipid domains localization phosphoinositide...

View article titled, Subcellular localization of phosphatidylinositol 4,5-bisphosphate using the pleckstrin homology domain of phospholipase C δ1

Articles

Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo

Wendy A. KIMBER, Laura TRINKLE-MULCAHY, Peter C. F. CHEUNG, Maria DEAK, Louisa J. MARSDEN, Agnieszka KIELOCH, Stephen WATT, Ronald T. JAVIER, Alex GRAY, C. Peter DOWNES, John M. LUCOCQ, Dario R. ALESSI

Biochem J (2002) 361 (3): 525–536.

https://doi.org/10.1042/bj3610525

Published: 25 January 2002

... (PH)-domain-containing proteins, termed ‘ ta ndem- P H-domain-containing p rotein- 1 ’ (TAPP1) and TAPP2, which interacted in vitro with high affinity with PtdIns(3,4) P 2 , but did not bind PtdIns(3,4,5) P 3 or other phosphoinositides. In the present study we demonstrate that stimulation of Swiss 3T3...

View article titled, Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo

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Articles

AtPIP5K1, an Arabidopsis thaliana phosphatidylinositol phosphate kinase, synthesizes PtdIns(3,4)P2 and PtdIns(4,5)P2 in vitro and is inhibited by phosphorylation

Tomas WESTERGREN, Stephen K. DOVE, Marianne SOMMARIN, Christophe PICAL

Biochem J (2001) 359 (3): 583–589.

https://doi.org/10.1042/bj3590583

Published: 25 October 2001

... ). 24 4 2001 26 7 2001 24 8 2001 The Biochemical Society, London ©2001 2001 lipid kinase phosphoinositide plant regulation Abbreviations used: GST, glutathione S-transferase; IPTG, isopropyl β- d -thiogalactoside; PIPkin, PtdIns P kinase; PKA, protein kinase...

View article titled, AtPIP5K1, an Arabidopsis thaliana phosphatidylinositol phosphate kinase, synthesizes PtdIns(3,4)P2 and PtdIns(4,5)P2 in vitro and is inhibited by phosphorylation

Articles

FYVE zinc-finger proteins in the plant model Arabidopsis thaliana: identification of PtdIns3P-binding residues by comparison of classic and variant FYVE domains

Rikke B. JENSEN, Tanja LACOUR, Jakob ALBRETHSEN, Michael NIELSEN, Karen SKRIVER

Biochem J (2001) 359 (1): 165–173.

https://doi.org/10.1042/bj3590165

Published: 24 September 2001

...Rikke B. JENSEN; Tanja LACOUR; Jakob ALBRETHSEN; Michael NIELSEN; Karen SKRIVER Classic FYVE zinc-finger domains recognize the phosphoinositide signal PtdIns3 P and share the basic (R/K) 1 (R/K)HHCR 6 (single-letter amino acid codes) consensus sequence. This domain is present in predicted PtdIns3 P...

View article titled, FYVE zinc-finger proteins in the plant model Arabidopsis thaliana: identification of PtdIns3P-binding residues by comparison of classic and variant FYVE domains

Articles

Targeting mutants of PTEN reveal distinct subsets of tumour suppressor functions

Nick R. LESLIE, Deborah BENNETT, Alex GRAY, Ian PASS, Khe HOANG-XUAN, C. Peter DOWNES

Biochem J (2001) 357 (2): 427–435.

https://doi.org/10.1042/bj3570427

Published: 09 July 2001

...Nick R. LESLIE; Deborah BENNETT; Alex GRAY; Ian PASS; Khe HOANG-XUAN; C. Peter DOWNES The tumour suppressor protein PTEN (phosphatase and tensin homolog deleted on chromosome 10) is a lipid phosphatase which can antagonize the phosphoinositide 3-kinase (PI 3-kinase) signalling pathway, promoting...

View article titled, Targeting mutants of PTEN reveal distinct subsets of tumour suppressor functions

Articles

Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins

David J. GILLOOLY, Anne SIMONSEN, Harald STENMARK

Biochem J (2001) 355 (2): 249–258.

https://doi.org/10.1042/bj3550249

Published: 06 April 2001

...David J. GILLOOLY; Anne SIMONSEN; Harald STENMARK PtdIns3 P is a phosphoinositide 3-kinase product that has been strongly implicated in regulating membrane trafficking in both mammalian and yeast cells. PtdIns3 P has been shown to be specifically located on membranes associated with the endocytic...

View article titled, Cellular functions of phosphatidylinositol 3-phosphate and FYVE domain proteins

Articles

Sac phosphatase domain proteins

William E. HUGHES, Frank T. COOKE, Peter J. PARKER

Biochem J (2000) 350 (2): 337–352.

https://doi.org/10.1042/bj3500337

Published: 23 August 2000

... the mammalian phosphoinositide 5-phosphatase synaptojanin and the yeast synaptojanin homologues Inp51p, Inp52p and Inp53p. These proteins therefore contain both Sac phosphatase and 5-phosphatase domains. This review describes the Sac phosphatase domain-containing proteins and their actions, with particular...

View article titled, Sac phosphatase domain proteins

Articles

Signal-dependent membrane targeting by pleckstrin homology (PH) domains

Mark A. LEMMON, Kathryn M. FERGUSON

Biochem J (2000) 350 (1): 1–18.

https://doi.org/10.1042/bj3500001

Published: 09 August 2000

..., their only clearly demonstrated physiological function to date is to bind membrane phosphoinositides. The PH domain from phospholipase C-δ 1 binds specifically to PtdIns(4,5) P 2 and its headgroup, and has become a valuable tool for studying cellular PtdIns(4,5) P 2 functions. More recent developments have...

View article titled, Signal-dependent membrane targeting by pleckstrin homology (PH) domains

Articles

DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides

Simon DOWLER, Richard A. CURRIE, C. Peter DOWNES, Dario R. ALESSI

Biochem J (1999) 342 (1): 7–12.

https://doi.org/10.1042/bj3420007

Published: 10 August 1999

... phosphorylated proteins and 3-phosphoinositides and may therefore play a role in regulating the location and/or activity of such proteins(s) in response to agonists that elevate PtdIns(3,4,5) P 3 and PtdIns(3,4) P 2 . 1 To whom correspondence should be addressed (e-mail sjdowler@bad.dundee.ac.uk...

View article titled, DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides

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