... cyclases and phosphodiesterases (PDEs) is critical. Here, we demonstrate that a putative stand-alone EAL domain PDE from Vibrio cholerae ( Vc EAL) is capable to degrade both the second messenger c-di-GMP and hybrid 3′3′-cyclic GMP–AMP (cGAMP). To unveil their degradation mechanism, we have determined high...

.... Mycobacterium smegmatis encodes a GAF (mammalian c G MP-regulated phosphodiesterases, Anabaena a denylyl cyclases and Escherichia coli transcription activator F hlA) domain containing bifunctional enzyme DcpA ( d iguanylate c yclase and p hosphodiesterase A ) that catalyzes the synthesis and hydrolysis of c-di...

... putative enzymes — one phosphodiesterase (gPDE) and two nucleotidyl cyclases (gNC1 and gNC2) — that should be responsible for the regulation of cAMP in G. lamblia . Later, an RT-PCR assay confirmed that these three genes are expressed in trophozoites. The bioinformatic analysis indicated that gPDE...

...Mathew Traini; Raani Kumaran; Morten Thaysen-Andersen; Maaike Kockx; Wendy Jessup; Leonard Kritharides Sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is a recently identified phosphodiesterase, which is a secreted N -linked glycoprotein. SMPDL3A is highly homologous to acid sphingomyelinase...

...Moses Xie; Brigitte Blackman; Colleen Scheitrum; Delphine Mika; Elise Blanchard; Tao Lei; Marco Conti; Wito Richter PDE4s (type 4 cyclic nucleotide phosphodiesterases) are divided into long and short forms by the presence or absence of conserved N-terminal domains termed UCRs (upstream conserved...

...Sonya Bader; Arjan Kortholt; Peter J. M. Van Haastert The Dictyostelium discoideum genome uncovers seven cyclic nucleotide PDEs (phosphodiesterases), of which six have been characterized previously and the seventh is characterized in the present paper. Three enzymes belong to the ubiquitous class I...

...Adam Lerner; Paul M. Epstein The cAMP signalling pathway has emerged as a key regulator of haematopoietic cell proliferation, differentiation and apoptosis. In parallel, general understanding of the biology of cyclic nucleotide PDEs (phosphodiesterases) has advanced considerably, revealing...

...Oliver SCHILLING; Andreas VOGEL; Brenda KOSTELECKY; Hugo NATAL da LUZ; Daniel SPEMANN; Bettina SPÄTH; Anita MARCHFELDER; Wolfgang TRÖGER; Wolfram MEYER-KLAUCKE ZiPD (zinc phosphodiesterase; synonyms are ElaC, ecoZ, RNaseZ and 3′ tRNase) and the iron-dependent redox enzyme FlRd (flavorubredoxin...

... (phosphodiesterase 4D3). In vitro and cellular experiments demonstrate that PKA-phosphorylation of PDE4D3 on Ser-13 increases the affinity of PDE4D3 for mAKAP. Our data suggest that activation of mAKAP-anchored PKA enhances the recruitment of PDE4D3, allowing for quicker signal termination. 1 These authors...

...Anisoara CIMPEAN; Cristiana STEFAN; Rik GIJSBERS; Willy STALMANS; Mathieu BOLLEN The nucleotide pyrophosphatases/phosphodiesterases NPP1 and NPP2/autotaxin are structurally related eukaryotic ecto-enzymes, but display a very different substrate specificity. NPP1 releases nucleoside 5...

...Lee Ann JOHNSTON; Suat ERDOGAN; York Fong CHEUNG; Michael SULLIVAN; Rachael BARBER; Martin J. LYNCH; George S. BAILLIE; Gino VAN HEEKE; David R. ADAMS; Elaine HUSTON; Miles D. HOUSLAY PDE4A7 is an isoform encoded by the human PDE4A cAMP-specific phosphodiesterase gene that fails to hydrolyse cAMP...

...Maximiliano A. D'ANGELO; Santiago SANGUINETI; Jeffrey M. REECE; Lutz BIRNBAUMER; Héctor N. TORRES; Mirtha M. FLAWIÁ Compartmentalization of cAMP phosphodiesterases plays a key role in the regulation of cAMP signalling in mammals. In the present paper, we report the characterization and subcellular...

...Malcolm SHEPHERD; Theresa McSORLEY; Aileen E. OLSEN; Lee Ann JOHNSTON; Neil C. THOMSON; George S. BAILLIE; Miles D. HOUSLAY; Graeme B. BOLGER We have isolated cDNAs encoding PDE4B4, a new cAMP-specific phosphodiesterase (PDE4) isoform with novel properties. The amino acid sequence of PDE4B4...

...Takashi SASAKI; Jun KOTERA; Kenji OMORI cDNA species coding for novel variants of cyclic-AMP-specific phosphodiesterases (PDEs), namely the PDE7B family, were isolated from rats and characterized. Rat PDE7B1 (RNPDE7B1) was composed of 446 amino acid residues. Rat PDE7B2 (RNPDE7B2) and PDE7B3...

...Stephen H. TSANG; Clyde K. YAMASHITA; Kentaro DOI; Daniel J. SALCHOW; Nicole BOUVIER; Monica MENDELSOHN; Peter GOURAS; Debora B. FARBER; Stephen P. GOFF The inhibitory rod cGMP phosphodiesterase γ subunit (PDEγ) is a major component of the photoresponse and is required to support rod integrity...