...Wanjun Qi; Iain W. Manfield; Stephen P. Muench; Alison Baker Phosphorus is an essential macronutrient for plant growth and is deficient in ∼50% of agricultural soils. The transcription factor phosphate starvation response 1 (PHR1) plays a central role in regulating the expression of a subset...

... involved in the phosphate signal transduction (PHO) pathway were up-regulated in the Δpho92 strain, as were genes regulated by Pho4, which is an important transcription factor in the PHO pathway. To identify the exact mechanism of Pho92 action with respect to phosphate metabolism, we investigated...

... that the inhibitory effects of CypD inhibition only manifest when P i (inorganic phosphate) is present, and that inhibition is lost when P i is replaced by As i (inorganic arsenate) or V i (inorganic vanadate). To test this, liver mitochondria were isolated from wild-type and CypD-deficient ( Ppif −/− ) mice...

... activity that facilitates the passage of phosphate and possibly pyrophosphate across the peroxisomal membrane. By reconstitution of peroxisomal membrane proteins in proteoliposomes, some kinetic parameters of the transporter could be established in vitro . The transporter can be distinguished from...

...Richard GRIEßLER; Sabato D'AURIA; Reinhard SCHINZEL; Fabio TANFANI; Bernd NIDETZKY Maltodextrin phosphorylase from Escherichia coli (MalP) is a dimeric protein in which each ≈ 90-kDa subunit contains active-site pyridoxal 5ʹ-phosphate. To unravel factors contributing to the stability of MalP...

... also analysed. Using a technique different to that used previously we show that both isoforms also affect the amylopectin, but in a way that is different to when both isoforms are repressed together. Key words: amylopectin branching, amylopectin structure, phosphate, starch granule, starch synthesis...