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Keywords: nuclear localization
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Articles
Biochem J (2016) 473 (24): 4593–4607.
Published: 09 December 2016
..., in vitro . We report that FGF10 harbours two putative nuclear localization sequences (NLSs), termed NLS1 and NLS2, which individually or co-operatively promote nuclear translocation of FGF10. Furthermore, FGF10 localizes to a subset of dense fibrillar components of the nucleolus. G138E falls within NLS1...
Includes: Supplementary data
Articles
Biochem J (2015) 468 (2): 345–352.
Published: 22 May 2015
... of cytosol-specific markers GAPDH and NF-kB p65 were consistent whether the cells expressed CHD5 endogenously or by overexpression ( Figure 1 B). Figure 1 Nuclear localization of CHD5: Western analysis Various NB cell lines were subjected to SDS/PAGE (4%–12% gels) and probed with fraction-specific...
Includes: Supplementary data
Articles
Biochem J (2014) 458 (2): 375–385.
Published: 14 February 2014
... the trafficking of the toxin within cells. Observations made in this regard revealed that the abrin A chain, after being released into the cytosol, is sequestered into the nucleus through interaction with a cellular protein of ~25 kDa, BASP1 (brain acid-soluble protein 1). The nuclear localization of the A chain...
Includes: Supplementary data
Articles
Biochem J (2010) 432 (1): 77–87.
Published: 25 October 2010
...-specific N-terminus of Fbw7α are phosphorylated in a PKC-dependent manner, both in vitro and in mammalian cells (Ser 10 and Ser 18 ). Mutational analyses reveal that phosphorylation of Fbw7α at Ser 10 can regulate its nuclear localization. Cancer-associated mutations in nearby residues (K11R...
Articles
Biochem J (2008) 414 (3): 419–429.
Published: 27 August 2008
... 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 Aspergillus nidulans calcium signalling cation homoeostasis cell-wall integrity nuclear localization transcription factor Rapid adaptation to different environmental conditions, including appropriate...
Includes: Supplementary data
Articles
Biochem J (2007) 408 (3): 335–345.
Published: 28 November 2007
... of these authors ( shears@niehs.nih.gov or mayr@uke.uni-hamburg.de ). 19 3 2007 18 7 2007 17 8 2007 17 8 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 euchromatin Ins(1,3,4,5,6) P 5 2-kinase (IP5K) in situ hybridization nuclear localization...
Includes: Supplementary data
Articles
Biochem J (2007) 403 (3): 397–407.
Published: 12 April 2007
... also directly acetylates Max in vitro at these three residues. Interestingly, the three Max residues acetylated in vivo and in vitro by p300 are important for Max nuclear localization and Max-mediated suppression of Myc transactivation. These results uncover novel post-translational modifications...
Includes: Supplementary data
Articles
Biochem J (2007) 402 (2): 359–366.
Published: 12 February 2007
...Shih-Ming Huang; Sheng-Ping Huang; Sung-Ling Wang; Pei-Yao Liu Zac1, a novel seven-zinc-finger transcription factor, preferentially binds GC-rich DNA elements and has intrinsic transactivation activity. To date, the NLS (nuclear localization signal) of Zac1 has not been empirically determined. We...
Articles
Biochem J (2005) 387 (3): 659–667.
Published: 26 April 2005
... Society, London 2005 Cdk cell cycle kinase myeloid cell leukaemia-1 (Mcl-1) nuclear localization proteolysis The Bcl-2 family of proteins are known regulators of cell death and survival, acting as regulators of membrane integrity at both the mitochondria and the endoplasmic reticulum...
Articles
Biochem J (2004) 378 (1): 169–176.
Published: 15 February 2004
... subcellular localization in cultured neurons suggest different functions of HDGF and HRP-3 within single cells. Key words: brain development, cell proliferation, HDGF-related protein-3 (HRP-3), hepatoma-derived growth factor (HDGF), nuclear localization, survival factor. INTRODUCTION HDGF (hepatoma-derived...
Articles
Biochem J (2003) 373 (1): 167–178.
Published: 01 July 2003
... analysis revealed that ZHX3 is a ubiquitous transcriptional repressor that is localized in nuclei and functions as a dimer. Lastly, the dimerization domain, the interaction domain with NF-YA, and the repressor domain are mapped to a region including the HD1 region, and two nuclear localization signals...
Articles
Biochem J (2002) 366 (2): 491–500.
Published: 01 September 2002
... proteins, bHRP-4–green fluorescent protein and mHDGF–green fluorescent protein, in HEK-293 cells demonstrates nuclear localization of the proteins. bHRP-4 and mHDGF bind to the glycosaminoglycans heparin and heparan sulphate, but not to chondroitin sulphate. Affinity constants determined...
Articles
Biochem J (2001) 355 (2): 473–479.
Published: 06 April 2001
... isoform has a perinuclear localization, and is associated with the cytoskeleton; this targeting, due to signals within the 3′-untranslated region (3′-UTR), facilitates nuclear localization of MT-1 during S-phase [Levadoux, Mahon, Beattie, Wallace and Hesketh (1999) J. Biol. Chem. 274 , 34961-34966]. Using...
Articles
Biochem J (2000) 349 (2): 443–453.
Published: 10 July 2000
... poly-His-tagged penta-ubiquitin. Moreover, it released free ubiquitin from poly-ubiquitinated protein conjugates of rabbit reticulocytes. In addition, UBP109 localized to both the cytoplasm and the nucleus and, among three putative nuclear localization sequences, only the one located near the C...
Articles
Biochem J (2000) 346 (3): 587–591.
Published: 07 March 2000
... PIPkin has no obvious nuclear localization sequence, and a detailed analysis of the localization of chimaeras and mutants of the α (cytosolic) and β PIPkins shows that the nuclear localization requires the presence of a 17-amino-acid length of α-helix (α-helix 7) that is specific to the β isoform...