...) synthesis nitrogen starvation sphingolipid G 0 /G 1  cells were subcultured in CSM containing [ 3 H]palmitic acid (5 μCi/ml) in the absence or presence of 5 mM β-chloroalanine. At time zero, the average cell number was (1.0–1.2)×10 6  cells/ml. After 20 min, the cells were washed with PBS...

... domain. 1 To whom correspondence should be addressed (e-mail cullin@cgm.cnrs-gif.fr ). 10 9 1998 6 11 1998 11 12 1998 The Biochemical Society, London © 1999 1999 binding assay nitrogen starvation non-Mendelian inheritance Saccharomyces cerevisiae two-hybrid system...