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Keywords: mutagenesis
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Biochem J (2023) 480 (9): 573–585.
Published: 04 May 2023
... of fluorine at 2′ position of nucleoside enhances the chemical stability of the glycosidic bond by destabilizing the transition state of spontaneous depurination, thereby deterring the degradation of N7-alkylG adducts [ 11 , 13 ]. alkyl-formamidopyrimidine alkylation damage fluorination mutagenesis...
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Biochem J (2021) 478 (9): 1769–1781.
Published: 10 May 2021
... by Portland Press Limited on behalf of the Biochemical Society 2021 8-oxoadenine DNA polymerase geometric selection mutagenesis translesion synthesis Human DNA is under constant threat from both endogenous and exogenous sources, and reactive oxygen species are one of the major DNA damaging...
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Biochem J (2020) 477 (24): 4797–4810.
Published: 24 December 2020
... predominantly pairs with cytosine to promote A to G mutations. Despite the known promutagenicity of the major deaminated purines, structures of DNA polymerase bypassing these lesions have not been reported. To gain insights into the deaminated-induced mutagenesis, we solved crystal structures of human DNA...
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Biochem J (2020) 477 (23): 4543–4558.
Published: 03 December 2020
... dithiothreitol), aliquoted and flash frozen in liquid nitrogen to store at −80°C. © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 mutagenesis nitrogen mustards polymerase eta translesion synthesis Monoacetylation of commercially available...
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Biochem J (2020) 477 (15): 2859–2871.
Published: 12 August 2020
... to the lesion's conformational flexibility that enables Hoogsteen base pairing with dATP in the confines of DNA polymerases. The mutagenesis mechanism of oxoA, which preferentially causes A to C transversions, remains poorly characterized. While structures for oxoA bypass by human DNA polymerases are available...
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Biochem J (2020) 477 (5): 937–951.
Published: 06 March 2020
... 2 2020 7 2 2020 10 2 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 cisplatin DNA intrastrand cross-link DNA polymerase mutagenesis translesion synthesis Cisplatin ( cis -diamminedichloroplatinum(II)) is one...
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Biochem J (2019) 476 (7): 1109–1119.
Published: 10 April 2019
... through site-directed mutagenesis. Amino acid substitutions at the sites involved in binding of the Fe(II) cofactor, 2OG cosubstrate and (2 S )- N ε -trimethyllysine substrate provide a basic insight into the binding requirements that determine an efficient TMLH-catalyzed conversion of (2 S )- N ε...
Includes: Supplementary data
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Biochem J (2018) 475 (2): 415–428.
Published: 23 January 2018
... The Author(s) 2018 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . anomalous dispersion bacterial chitinase secretion crystal structure mutagenesis peptidoglycan...
Includes: Supplementary data
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Biochem J (2017) 474 (11): 1837–1852.
Published: 16 May 2017
... Poznański ( jarek@ibb.waw.pl ) 15 11 2016 7 4 2017 13 4 2017 13 4 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 acrolein AlkB dioxygenase DNA repair mutagenesis substrate binding Acrolein (ACR), a highly...
Includes: Supplementary data
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Biochem J (2016) 473 (14): 2073–2085.
Published: 12 July 2016
... eight conserved cysteine residues as potential co-ordinating ligands for Fe–S clusters but their functional importance and the type of bound clusters is unclear. In the present study, we use a combination of mutagenesis, cell biological and biochemical as well as UV–visible, EPR and Mössbauer...
Includes: Supplementary data
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Biochem J (2014) 459 (2): 289–299.
Published: 28 March 2014
... catalytically important residues. Site-directed mutagenesis experiments confirmed the role of the identified residues, and demonstrated the importance of selected acidic residues and a conserved G 108 NYFWTHYFF 117 motif. The mutated isomerases were assayed both in vivo by quantification of cycloeucalenol...
Includes: Supplementary data
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Biochem J (2013) 452 (1): 121–129.
Published: 25 April 2013
... of whole-cell patch voltage-clamp, immunoprecipitation, Western blotting and mutagenesis. We found that the hSKCa1 current was inhibited by the broad-spectrum PTK inhibitor genistein, the selective EGFR (epidermal growth factor receptor) kinase inhibitors T25 (tyrphostin 25) and AG556 (tyrphostin AG 556...
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Biochem J (2012) 444 (2): 199–204.
Published: 11 May 2012
... Biochemical Society 2012 cytochrome c oxidase energy coupling mutagenesis proton channel subunit I yeast The membrane preparation was diluted to 1.8 mg of protein/ml in 50 mM KPi, pH 8.0, and solubilized by incubation on ice for 30 min with 2% (w/v) DDM ( n -dodecyl β- D -maltoside...
Includes: Supplementary data
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Biochem J (2012) 443 (3): 841–850.
Published: 16 April 2012
.... chimaeric receptor evolution mutagenesis orphan G-protein-coupled receptor (GPCR) structure–function relationship GPR34 is an orphan GPCR (G-protein-coupled receptor) and was first discovered by mining GenBank® for novel GPCR sequences and homology cloning. It has been assigned to the human X...
Includes: Supplementary data
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Biochem J (2011) 438 (1): 155–164.
Published: 27 July 2011
... 2011 1 6 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 cytokine receptor interleukin-27 receptor α (IL27R) Janus kinase/signal transducer and activator of transcription (JAK/STAT) mutagenesis transformation In order to directly compare...
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Biochem J (2011) 437 (2): 243–253.
Published: 28 June 2011
...) in an apo state and in complex with the non-hydrolysable substrate α,β-imido dUTP or dUMP product. Alanine-replacement mutagenesis of the active-site residues revealed seven residues important for activity including the conserved triad Asp 87 /Arg 137 /Asp 85 . The Y88A mutant protein was equally active...
Includes: Multimedia, Supplementary data
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Biochem J (2009) 421 (2): 231–241.
Published: 26 June 2009
...] is the regulatory enzyme for the biosynthesis of shikonin, a naphthoquinone pigment, and was utilized in the present study as a representative of membrane-type AS-PTs to clarify the function of this enzyme family at the molecular level. Site-directed mutagenesis of LePGT1 with a yeast expression system indicated...
Includes: Supplementary data
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Biochem J (2009) 420 (2): 201–209.
Published: 13 May 2009
..., serine and arginine residues. Site-directed mutagenesis and kinetic analysis strongly suggest that Lys 57 is important for the fumarate-induced activation and quaternary structural organization of the enzyme. Lys 57 mutant enzymes demonstrate a reduction of K m and an elevation of k cat following...
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Biochem J (2008) 415 (3): 449–454.
Published: 15 October 2008
..., suggesting a two-metal-ion mechanism as is known for class II and class III adenylate cyclases. Twelve residues which are essential for catalysis were identified by mutagenesis of a total of 20 polar residues conserved in all class I adenylate cyclases. Five essential residues (Ser 103 , Ser 113 , Asp 114...
Includes: Supplementary data
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Biochem J (2008) 413 (1): 135–142.
Published: 12 June 2008
.... Mutagenesis of Arg 347 to other amino acids also decreased the inhibitory potency, with aspartate producing near total loss of CFTR inh -172 activity. The results of the present study provide evidence that CFTR inh -172 interacts directly with CFTR, and that Arg 347 is important for the interaction. 1...
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Biochem J (2008) 412 (1): 103–112.
Published: 25 April 2008
... 1 2008 31 1 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 allosteric protein G-protein-coupled receptor (GPCR) inverse agonist M 3 muscarinic acetylcholine receptor (M 3 R) mutagenesis signal transduction CCh (carbachol), atropine sulfate...
Includes: Supplementary data
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Biochem J (2006) 397 (2): 297–304.
Published: 28 June 2006
.... In the present study, we investigated whether TWEAK binding to this CRD was dependent on selected evolutionarily conserved amino acid residues by using a site-specific mutagenesis approach and several different ligand-binding assays. Our results indicate that three residues within the predicted Fn14 CRD A1...
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Biochem J (2006) 397 (1): 47–52.
Published: 14 June 2006
... xanthus protoporphyrin (IX) ferrochelatase ligates a [2Fe-2S] cluster via cysteine residues present in an internal segment. Site-directed mutagenesis of this ferrochelatase demonstrates that changing one cysteine ligand into serine results in loss of the cluster, but unlike eukaryotic protoporphyrin (IX...
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Biochem J (2005) 392 (3): 601–606.
Published: 06 December 2005
... correspondence should be addressed (email wojcikir@upstate.edu ). 14 6 2005 19 8 2005 1 9 2005 1 9 2005 The Biochemical Society, London 2005 Ca 2+ inositol 1,4,5-trisphosphate receptor (IP 3 R) ER-associated degradation (ERAD) mutagenesis αT3-1 cells ubiquitination...
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Biochem J (2005) 391 (1): 105–114.
Published: 26 September 2005
... polymerase with a mixture of dATP, dTTP and dGTP and religating with T4 DNA ligase. The pTYB12- ldpex5-203 – 347 vector was generated from the pTYB12- ldpex5-203 – 391 construct using a site-directed mutagenesis approach to introduce a stop codon immediately downstream of the codon encoding Phe 347...
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Biochem J (2005) 390 (2): 395–405.
Published: 23 August 2005
... coupled site-directed mutagenesis of the recombinant cABC I with a structural model of the enzyme–substrate complex in order to investigate in detail the roles of active site amino acids in the catalytic action of the enzyme. The putative catalytic residues His-501, Tyr-508, Arg-560 and Glu-653 were...
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Biochem J (2005) 389 (1): 173–180.
Published: 21 June 2005
... Society, London 2005 epimerase mutagenesis specificity structure substrate UDP- N -acetylglucosamine Most studies concerning C4 epimerases have focused on refining the catalytic mechanism involved [ 9 – 16 ], but relatively few have examined the molecular basis for substrate specificity...
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Biochem J (2005) 387 (3): 849–858.
Published: 26 April 2005
... cytoplasmic domain filamin A GPIb/V/IX mutagenesis platelet Platelet adhesion and aggregation at sites of vascular injury is essential for the arrest of bleeding and is dependent on the synergistic contribution of multiple adhesion receptor–ligand interactions. Of central importance...
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Biochem J (2005) 387 (1): 101–108.
Published: 22 March 2005
... domains supported α9β1-dependent cell adhesion. Recognition by both α4β1 and α4β7 of ADAM7 and ADAM28 was activation-dependent, requiring either the presence of Mn 2+ or an activating monoclonal antibody for cell attachment. Charge-to-alanine mutagenesis experiments revealed that the same residues within...
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Biochem J (2005) 386 (3): 453–460.
Published: 08 March 2005
... the conformational changes in the peptide-binding domain of Hsp70. These conformational changes will then stabilize the Hsp70 and peptide substrate complexes [ 1 , 12 ] and allow the subsequent protein folding to occur. crystal structure heat-shock protein 40 (Hsp40) Hsp70 mutagenesis substrate binding...
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Biochem J (2004) 384 (1): 87–92.
Published: 09 November 2004
...-hypertensives and anti-inflammatories. hOAT4-mediated transport of the organic anion oestrone sulphate in COS-7 cells was inhibited by the histidine-modifying reagent DEPC (diethyl pyrocarbonate). Therefore the role of histidine residues in the function of hOAT4 was examined by site-directed mutagenesis. All...
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