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Articles

The RED light is on! New tools for monitoring Ca 2+ dynamics in the endoplasmic reticulum and mitochondria

Saverio Marchi, Paolo Pinton

Biochem J (2014) 464 (1): e5–e6.

DOI: https://doi.org/10.1042/BJ20141183

Published: 23 October 2014

... The Authors Journal compilation © 2014 Biochemical Society 2014 aequorin Ca 2+ measurement endoplasmic reticulum fluorescent Ca 2+ indicator GCaMP mitochondrion Since the identification of the intense Ca 2+ connection that occurs between the ER (endoplasmic reticulum) and mitochondria...

View article titled, The RED light is on! New tools for monitoring Ca 2+ dynamics in the endoplasmic reticulum and mitochondria

Articles

Red fluorescent genetically encoded Ca 2+ indicators for use in mitochondria and endoplasmic reticulum

Jiahui Wu, David L. Prole, Yi Shen, Zhihong Lin, Aswini Gnanasekaran, Yingjie Liu, Lidong Chen, Hang Zhou, S. R. Wayne Chen, Yuriy M. Usachev, Colin W. Taylor, Robert E. Campbell

Biochem J (2014) 464 (1): 13–22.

DOI: https://doi.org/10.1042/BJ20140931

Published: 23 October 2014

... fluorescent genetically encoded Ca 2+ indicators with low affinities. These indicators enable monitoring of mitochondrial and ER Ca 2+ dynamics in combination with a green fluorescent indicator. endoplasmic reticulum (ER) fluorescence Ca 2+ imaging GCaMP mitochondrion multicolour imaging red...

View article titled, Red fluorescent genetically encoded Ca 2+ indicators for use in mitochondria and endoplasmic reticulum

Includes: Supplementary data

Articles

Metformin and respiratory chain complex I: the last piece of the puzzle?

Eric Fontaine

Biochem J (2014) 463 (3): e3–e5.

DOI: https://doi.org/10.1042/BJ20141020

Published: 10 October 2014

... metformin mitochondrion phenformin In 2000, two laboratories published independently in two different journals that metformin inhibited mitochondrial respiratory chain complex I [ 1 , 2 ]. Since then, more than ten different laboratories have reproduced this observation. However, although all...

View article titled, Metformin and respiratory chain complex I: the last piece of the puzzle?

Articles

Selective induction and subcellular distribution of ACONITASE 3 reveal the importance of cytosolic citrate metabolism during lipid mobilization in Arabidopsis

Mark A. Hooks, J. William Allwood, Joanna K. D. Harrison, Joachim Kopka, Alexander Erban, Royston Goodacre, Janneke Balk

Biochem J (2014) 463 (2): 309–317.

DOI: https://doi.org/10.1042/BJ20140430

Published: 22 September 2014

... to, if not much greater than, that in mitochondria [ 6 – 10 ]. cytosol glyoxylate cycle glyoxysome mitochondrion seedling The cytosolic location of AtACO3 and its importance in citrate metabolism support the operation of the classic glyoxylate cycle and not direct mitochondrial metabolism...

View article titled, Selective induction and subcellular distribution of ACONITASE 3 reveal the importance of cytosolic citrate metabolism during lipid mobilization in Arabidopsis

Includes: Supplementary data

Articles

Drp1 stabilizes p53 on the mitochondria to trigger necrosis under oxidative stress conditions in vitro and in vivo

Xing Guo, Hiromi Sesaki, Xin Qi

Biochem J (2014) 461 (1): 137–146.

DOI: https://doi.org/10.1042/BJ20131438

Published: 13 June 2014

... in medium were purified using Amicon Ultra 0.5 ml centrifugal filters (Millipore) and analysed by Western blotting with anti-HMGB1 antibody. dynamin-related protein 1 (Drp1) mitochondrion necrosis oxidative stress p53 ubiquitination Full-length Drp1, FLAG–p53, GFP–p53, HA...

View article titled, Drp1 stabilizes p53 on the mitochondria to trigger necrosis under oxidative stress conditions in vitro and in vivo

Includes: Supplementary data

Articles

Glutamyl-tRNA Gln amidotransferase is essential for mammalian mitochondrial translation in vivo

Lucía Echevarría, Paula Clemente, Rosana Hernández-Sierra, María Esther Gallardo, Miguel A. Fernández-Moreno, Rafael Garesse

Biochem J (2014) 460 (1): 91–101.

DOI: https://doi.org/10.1042/BJ20131107

Published: 25 April 2014

...), a mitochondrion-selective dye. Cells were visualized with a TCS SP2 laser-scanning confocal microscope (Leica) using a ×63 magnification 1.32 numerical aperture oil-immersion UV lens. GFP-fusion proteins were imaged using a 488 nm excitation wavelength and an emission collection wavelength of 510 nm (green...

View article titled, Glutamyl-tRNA Gln amidotransferase is essential for mammalian mitochondrial translation in vivo

Articles

Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site

Craig T. Armstrong, J. L. Ross Anderson, Richard M. Denton

Biochem J (2014) 459 (2): 369–381.

DOI: https://doi.org/10.1042/BJ20131664

Published: 28 March 2014

... energy metabolism E1 subunit mitochondrion α-oxoglutarate dehydrogenase (OGDH) The OGDH (2-oxoglutarate dehydrogenase) complex plays a central role in the regulation of eukaryotic intramitochondrial metabolism, particularly the citrate cycle. It is well established that its overall activity...

View article titled, Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site

Includes: Supplementary data

Articles

Frataxin-bypassing Isu1: characterization of the bypass activity in cells and mitochondria

Heeyong Yoon, Simon A. B. Knight, Alok Pandey, Jayashree Pain, Yan Zhang, Debkumar Pain, Andrew Dancis

Biochem J (2014) 459 (1): 71–81.

DOI: https://doi.org/10.1042/BJ20131273

Published: 14 March 2014

... frataxin iron–sulfur Isu1 scaffold mitochondrion Frataxin is a conserved mitochondrial protein implicated in causation of Friedreich's ataxia, an inherited neurodegenerative disease [ 1 ]. It is the deficiency of frataxin in certain tissues, such as the dorsal root ganglia and heart...

View article titled, Frataxin-bypassing Isu1: characterization of the bypass activity in cells and mitochondria

Includes: Supplementary data

Articles

Decrease in cytochrome c oxidase reserve capacity diminishes robustness of Drosophila melanogaster and shortens lifespan

Vladimir Klichko, Barbara H. Sohal, Svetlana N. Radyuk, William C. Orr, Rajindar S. Sohal

Biochem J (2014) 459 (1): 127–135.

DOI: https://doi.org/10.1042/BJ20131370

Published: 14 March 2014

... lifespan mitochondrion PCRs were performed using the following sets of primers. The 10664-total set includes the forward 5′-GCGCCAA-GGAGCAGGGAGAC-3′ (10664-F) and the reverse 5′-GGC-GGCGCAGAAGAGGAGTG-3′ (10664-R) primers, both of which are located in the coding region and consequently allow...

View article titled, Decrease in cytochrome c oxidase reserve capacity diminishes robustness of Drosophila melanogaster and shortens lifespan

Includes: Supplementary data

Articles

Effects of carbonic anhydrase-related protein VIII on human cells harbouring an A8344G mitochondrial DNA mutation

Tze-Kai Wang, Che-Kun Cheng, Tang-Hao Chi, Yi-Shing Ma, Shi-Bei Wu, Yau-Huei Wei, Mingli Hsieh

Biochem J (2014) 459 (1): 149–160.

DOI: https://doi.org/10.1042/BJ20131235

Published: 14 March 2014

... of CA8 desensitizes mutant MERRF cybrids to apoptotic stress. Phosphorylated Akt is involved in the protection of cells by CA8 in MERRF cybrids. apoptosis autophagy carbonic anhydrase-related protein VIII (CA8) mitochondrion mtDNA point mutation myoclonus epilepsy associated with ragged-red...

View article titled, Effects of carbonic anhydrase-related protein VIII on human cells harbouring an A8344G mitochondrial DNA mutation

Articles

TIGAR's promiscuity

Juan P. Bolaños

Biochem J (2014) 458 (3): e5–e7.

DOI: https://doi.org/10.1042/BJ20140087

Published: 28 February 2014

... in health and disease. 1 email jbolanos@usal.es 21 1 2014 22 1 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 2,3-bisphosphoglycerate phosphatase cancer glycolysis haemoglobin hypoxia mitochondrion oxygen sensing TIGAR [TP53 (tumour...

View article titled, TIGAR's promiscuity

Articles

Enhancing the peroxidase activity of cytochrome c by mutation of residue 41: implications for the peroxidase mechanism and cytochrome c release

Tracy M. Josephs, Ian M. Morison, Catherine L. Day, Sigurd M. Wilbanks, Elizabeth C. Ledgerwood

Biochem J (2014) 458 (2): 259–265.

DOI: https://doi.org/10.1042/BJ20131386

Published: 14 February 2014

... is not essential. apoptosis cardiolipin cytochrome c mitochondrion omega loop peroxidase Cytochrome c plays an essential role in the intrinsic apoptosis pathway, with apoptosome formation and subsequent caspase activation in the cytosol triggered by release of cytochrome c from...

View article titled, Enhancing the peroxidase activity of cytochrome c by mutation of residue 41: implications for the peroxidase mechanism and cytochrome c release

Includes: Supplementary data

Articles

Dynamics of mitochondrial Ca 2+ uptake in MICU1-knockdown cells

Sergio de la Fuente, Jessica Matesanz-Isabel, Rosalba I. Fonteriz, Mayte Montero, Javier Alvarez

Biochem J (2014) 458 (1): 33–40.

DOI: https://doi.org/10.1042/BJ20131025

Published: 20 January 2014

... mitochondrial (MCU) Ca 2+ uptake protein 1 mitochondrial (MICU1) knockdown mitochondrion In recent years, a number of new proteins involved in mitochondrial Ca 2+ transport have been discovered [ 1 ]. MICU1 (Ca 2+ uptake protein 1, mitochondrial) was the first one known to be involved...

View article titled, Dynamics of mitochondrial Ca 2+ uptake in MICU1-knockdown cells

Includes: Supplementary data

Articles

Loss of TRPML1 promotes production of reactive oxygen species: is oxidative damage a factor in mucolipidosis type IV?

Jessica Coblentz, Claudette St. Croix, Kirill Kiselyov

Biochem J (2014) 457 (2): 361–368.

DOI: https://doi.org/10.1042/BJ20130647

Published: 20 December 2013

... channel permeable to cations, including Fe 2+ . Our data suggest that TRPML1 redistributes Fe 2+ between the lysosomes and the cytoplasm. Loss of TRPML1 leads to production of reactive oxygen species, and to mitochondrial deterioration. iron lysosomal storage disease mitochondrion reactive...

View article titled, Loss of TRPML1 promotes production of reactive oxygen species: is oxidative damage a factor in mucolipidosis type IV?

Includes: Supplementary data

Articles

Mitochondrial fission mediates ceramide-induced metabolic disruption in skeletal muscle

Melissa E. Smith, Trevor S. Tippetts, Eric S. Brassfield, Braden J. Tucker, Adelaide Ockey, Adam C. Swensen, Tamil S. Anthonymuthu, Trevor D. Washburn, Daniel A. Kane, John T. Prince, Benjamin T. Bikman

Biochem J (2013) 456 (3): 427–439.

DOI: https://doi.org/10.1042/BJ20130807

Published: 22 November 2013

... (email benjamin_bikman@byu.edu ). Despite being commonly represented as small segmented structures, mitochondria are highly dynamic organelles undergoing continuous fission and fusion. Indeed, the word mitochondrion itself reveals the typical state of the mitochondrion ( mitos means thread...

View article titled, Mitochondrial fission mediates ceramide-induced metabolic disruption in skeletal muscle

Includes: Supplementary data

Articles

Investigating the function of [2Fe–2S] cluster N1a, the off-pathway cluster in complex I, by manipulating its reduction potential

James A. Birrell, Klaudia Morina, Hannah R. Bridges, Thorsten Friedrich, Judy Hirst

Biochem J (2013) 456 (1): 139–146.

DOI: https://doi.org/10.1042/BJ20130606

Published: 24 October 2013

... the terms of the Creative Commons Attribution Licence (CC-BY) ( http://creativecommons.org/licenses/by/3.0/ ) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. electron transport chain iron–sulfur cluster mitochondrion...

View article titled, Investigating the function of [2Fe–2S] cluster N1a, the off-pathway cluster in complex I, by manipulating its reduction potential

Includes: Supplementary data

Articles

Identification and characterization of mitochondrial Mia40 as an iron–sulfur protein

Michael P. Spiller, Swee Kim Ang, Efrain Ceh-Pavia, Karl Fisher, Qi Wang, Stephen E. J. Rigby, Hui Lu

Biochem J (2013) 455 (1): 27–35.

DOI: https://doi.org/10.1042/BJ20130442

Published: 13 September 2013

... cysteine redox state iron–sulfur cluster metal binding mitochondrion Figure 1 Structure, cofactor and dimerization of Mia40 ( A ) Schematic of the primary structure of C-terminal Mia40 with three disulfide bonds formed by the conserved cysteine motifs (left-hand panel) and the X-ray...

View article titled, Identification and characterization of mitochondrial Mia40 as an iron–sulfur protein

Articles

Mitochondrion-associated protein LRPPRC suppresses the initiation of basal levels of autophagy via enhancing Bcl-2 stability

Jing Zou, Fei Yue, Xianhan Jiang, Wenjiao Li, Jinglin Yi, Leyuan Liu

Biochem J (2013) 454 (3): 447–457.

DOI: https://doi.org/10.1042/BJ20130306

Published: 29 August 2013

...Jing Zou; Fei Yue; Xianhan Jiang; Wenjiao Li; Jinglin Yi; Leyuan Liu The mitochondrion-associated protein LRPPRC (leucine-rich pentatricopeptide repeat-containing) interacts with one of the microtubule-associated protein family members MAP1S (microtubule-associated protein 1 small form), originally...

View article titled, <span class="search-highlight">Mitochondrion</span>-associated protein LRPPRC suppresses the initiation of basal levels of autophagy via enhancing Bcl-2 stability

Articles

Physiological and pathological roles of mitochondrial SLC25 carriers

Manuel Gutiérrez-Aguilar, Christopher P. Baines

Biochem J (2013) 454 (3): 371–386.

DOI: https://doi.org/10.1042/BJ20121753

Published: 29 August 2013

...Manuel Gutiérrez-Aguilar; Christopher P. Baines The mitochondrion relies on compartmentalization of certain enzymes, ions and metabolites for the sake of efficient metabolism. In order to fulfil its activities, a myriad of carriers are properly expressed, targeted and folded in the inner...

View article titled, Physiological and pathological roles of mitochondrial SLC25 carriers

Articles

Mitochondrial respiratory chain complex I is inactivated by NADPH oxidase Nox4

Rafał Kozieł, Haymo Pircher, Manuela Kratochwil, Barbara Lener, Martin Hermann, Norbert A. Dencher, Pidder Jansen-Dürr

Biochem J (2013) 452 (2): 231–239.

DOI: https://doi.org/10.1042/BJ20121778

Published: 10 May 2013

... transport chain mitochondrion Nox4 oxidative stress senescence Cell growth and live confocal imaging were performed in eight-well chambered coverglasses (Nalge Nunc International). The cells were stained for 15 min in 200 μl of culture medium containing 100 μM TMRM (tetramethylrhodamine methyl...

View article titled, Mitochondrial respiratory chain complex I is inactivated by NADPH oxidase Nox4

Includes: Supplementary data

Articles

Evidence for novel aspects of Nox4 oxidase regulation of mitochondrial function and peroxide generation in an endothelial cell model of senescence

Michael S. Wolin

Biochem J (2013) 452 (2): e1–e2.

DOI: https://doi.org/10.1042/BJ20130484

Published: 10 May 2013

... pulmonary hypertension Am. J. Physiol. Lung Cell. Mol. Physiol. 2009 297 L184 L195 electron transport chain endothelium mitochondrion Nox4 oxidant signalling senescence A study reported in this issue of the Biochemical Journal by Kozieł et al. [ 1 ] defines several novel roles...

View article titled, Evidence for novel aspects of Nox4 oxidase regulation of mitochondrial function and peroxide generation in an endothelial cell model of senescence

Articles

The paradox of elongation factor 4: highly conserved, yet of no physiological significance?

Dejiu Zhang, Yan Qin

Biochem J (2013) 452 (2): 173–181.

DOI: https://doi.org/10.1042/BJ20121792

Published: 10 May 2013

... and discuss the directions future EF4 research should focus on. 3 12 2012 5 2 2013 6 2 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 back-translocation elongation factor 4 (EF4) (LepA) GTPase of unknown function 1 (Guf1) mitochondrion...

View article titled, The paradox of elongation factor 4: highly conserved, yet of no physiological significance?

Articles

Pathogenic mutations causing LBSL affect mitochondrial aspartyl-tRNA synthetase in diverse ways

Laura van Berge, Josta Kevenaar, Emiel Polder, Agnès Gaudry, Catherine Florentz, Marie Sissler, Marjo S. van der Knaap, Gert C. Scheper

Biochem J (2013) 450 (2): 345–350.

DOI: https://doi.org/10.1042/BJ20121564

Published: 15 February 2013

... were conducted as described in [ 17 ]. Aminoacylation assays were performed at 37°C as described in [ 4 ]. aminoacyl-tRNA synthetase aspartylation leukoencephalopathy missense mutation mitochondrion LBSL (leukoencephalopathy with brain stem and spinal cord involvement and lactate...

View article titled, Pathogenic mutations causing LBSL affect mitochondrial aspartyl-tRNA synthetase in diverse ways

Includes: Supplementary data

Articles

GTPase of the immune-associated nucleotide-binding protein 5 (GIMAP5) regulates calcium influx in T-lymphocytes by promoting mitochondrial calcium accumulation

Xi Lin Chen, Daniel Serrano, Marian Mayhue, Hans-Joachim Wieden, Jana Stankova, Guylain Boulay, Subburaj Ilangumaran, Sheela Ramanathan

Biochem J (2013) 449 (2): 353–364.

DOI: https://doi.org/10.1042/BJ20120516

Published: 14 December 2012

... 26 10 2012 26 10 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 calcium flux GTPase of the immune-associated nucleotide-binding protein 5 (GIMAP5) mitochondrion microtubule T-lymphocyte In the BB-DP (Biobreeding diabetes-prone) strain of rats...

View article titled, GTPase of the immune-associated nucleotide-binding protein 5 (GIMAP5) regulates calcium influx in T-lymphocytes by promoting mitochondrial calcium accumulation

Includes: Multimedia, Supplementary data

Articles

Nitric oxide inhibits succinate dehydrogenase-driven oxygen consumption in potato tuber mitochondria in an oxygen tension-independent manner

Vagner Simonin, Antonio Galina

Biochem J (2013) 449 (1): 263–273.

DOI: https://doi.org/10.1042/BJ20120396

Published: 07 December 2012

... should be addressed (email galina@bioqmed.ufrj.br , antoniogalina@yahoo.com.br or galina.antonio@gmail.com ). 6 3 2012 5 10 2012 8 10 2012 8 10 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 heterotrophic plant tissue mitochondrion nitric...

View article titled, Nitric oxide inhibits succinate dehydrogenase-driven oxygen consumption in potato tuber mitochondria in an oxygen tension-independent manner

Articles

Defective valyl-tRNA synthetase hampers the mitochondrial respiratory chain in Neurospora crassa

Margarida Duarte, Arnaldo Videira

Biochem J (2012) 448 (3): 297–306.

DOI: https://doi.org/10.1042/BJ20120963

Published: 21 November 2012

...-tRNA synthetase mitochondrion Neurospora crassa oxidative phosphorylation (OXPHOS) complex respiratory chain Mitochondria are vital organelles that provide energy to the cells mainly through OXPHOS (oxidative phosphorylation). Indeed a myriad of human pathologies have been associated...

View article titled, Defective valyl-tRNA synthetase hampers the mitochondrial respiratory chain in Neurospora crassa

Includes: Supplementary data

Articles

Persulfide formation on mitochondrial cysteine desulfurase: enzyme activation by a eukaryote-specific interacting protein and Fe–S cluster synthesis

Alok Pandey, Ramesh Golla, Heeyong Yoon, Andrew Dancis, Debkumar Pain

Biochem J (2012) 448 (2): 171–187.

DOI: https://doi.org/10.1042/BJ20120951

Published: 07 November 2012

... 24 8 2012 28 8 2012 28 8 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 cysteine desulfurase Fe–S cluster mitochondrion Nfs1p·Isd11p complex persulfide yeast Fe–S clusters are modular cofactors consisting of iron and sulfur liganded...

View article titled, Persulfide formation on mitochondrial cysteine desulfurase: enzyme activation by a eukaryote-specific interacting protein and Fe–S cluster synthesis

Includes: Supplementary data

Articles

Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components

Masato Ogura, Junko Yamaki, Miwako K. Homma, Yoshimi Homma

Biochem J (2012) 447 (2): 281–289.

DOI: https://doi.org/10.1042/BJ20120509

Published: 26 September 2012

... the terms of the Creative Commons Attribution Non-Commercial Licence ( http://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. cell death energy metabolism mitochondrion...

View article titled, Mitochondrial c-Src regulates cell survival through phosphorylation of respiratory chain components

Includes: Supplementary data

Articles

Mysterious Ca 2+ -independent muscular contraction: déjà vu

Andrey V. Kuznetsov, Rita Guzun, François Boucher, Rafaela Bagur, Tuuli Kaambre, Valdur Saks

Biochem J (2012) 445 (3): 333–336.

DOI: https://doi.org/10.1042/BJ20120439

Published: 13 July 2012

... © The Authors Journal compilation © 2012 Biochemical Society 2012 calcium-independent muscle contraction heart mitochondrion permeabilized myocyte respiration skeletal muscle With great interest, we have read the recent paper in the Biochemical Journal by Christopher Perry et al...

View article titled, Mysterious Ca 2+ -independent muscular contraction: déjà vu

Includes: Multimedia, Supplementary data

Articles

Mitochondrial free [Ca 2+ ] dynamics measured with a novel low-Ca 2+ affinity aequorin probe

Sergio de la Fuente, Rosalba I. Fonteriz, Pedro J. de la Cruz, Mayte Montero, Javier Alvarez

Biochem J (2012) 445 (3): 371–376.

DOI: https://doi.org/10.1042/BJ20120423

Published: 13 July 2012

...+ dynamics mitochondrion It has already been 20 years since the first measurements of [Ca 2+ ] M (mitochondrial [Ca 2+ ]) were made using targeted recombinant aequorin [ 1 ]. The original measurements were made using native aequorin and provided peak [Ca 2+ ] M values of 2–3 μM during histamine...

View article titled, Mitochondrial free [Ca 2+ ] dynamics measured with a novel low-Ca 2+ affinity aequorin probe

Articles

Effects of the novel mitochondrial protein mimitin in insulin-secreting cells

Katarzyna Hanzelka, Lukasz Skalniak, Jolanta Jura, Sigurd Lenzen, Ewa Gurgul-Convey

Biochem J (2012) 445 (3): 349–359.

DOI: https://doi.org/10.1042/BJ20111920

Published: 13 July 2012

... Gurgul-Convey.Ewa@mh-hannover.de ). 28 10 2011 8 5 2012 16 5 2012 16 5 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 ATP cytokine diabetes insulin-secreting cell mimitin mitochondrion Mimitin is a small 20 kDa mitochondrial protein...

View article titled, Effects of the novel mitochondrial protein mimitin in insulin-secreting cells

Articles

Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana

Hannah Birke, Florian H. Haas, Luit J. De Kok, Janneke Balk, Markus Wirtz, Rüdiger Hell

Biochem J (2012) 445 (2): 275–283.

DOI: https://doi.org/10.1042/BJ20120038

Published: 27 June 2012

... were ground in liquid nitrogen to a fine powder and stored at −80°C. © The Authors Journal compilation © 2012 Biochemical Society 2012 O -acetylserine(thiol)lyase cysteine cytochrome c oxidase (COX) electron transport chain mitochondrion sulfide toxicity Biosynthesis...

View article titled, Cysteine biosynthesis, in concert with a novel mechanism, contributes to sulfide detoxification in mitochondria of Arabidopsis thaliana

Includes: Supplementary data

Articles

Bax and Bcl-xL exert their regulation on different sites of the ceramide channel

Meenu N. Perera, Shang H. Lin, Yuri K. Peterson, Alicja Bielawska, Zdzislaw M. Szulc, Robert Bittman, Marco Colombini

Biochem J (2012) 445 (1): 81–91.

DOI: https://doi.org/10.1042/BJ20112103

Published: 15 June 2012

... colombini@umd.edu ). 29 11 2011 9 4 2012 11 4 2012 11 4 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 ABT-263 ABT-737 apoptosis Bcl-2 family protein ceramide analogue ceramide channel 2-methoxyantimicin A 3 mitochondrion...

View article titled, Bax and Bcl-xL exert their regulation on different sites of the ceramide channel

Includes: Supplementary data

Articles

The post-transcriptional life of mammalian mitochondrial RNA

Joanna Rorbach, Michal Minczuk

Biochem J (2012) 444 (3): 357–373.

DOI: https://doi.org/10.1042/BJ20112208

Published: 29 May 2012

... PNPase is directly involved in the poly(A) tail metabolism. mitochondrion ribosome biogenesis RNA degradation RNA polyadenylation RNA turnover According to the endosymbiotic theory, mitochondria originated from proteobacteria that were taken inside another cell (either by a primitive...

View article titled, The post-transcriptional life of mammalian mitochondrial RNA

Articles

Pyruvate fuels mitochondrial respiration and proliferation of breast cancer cells: effect of monocarboxylate transporter inhibition

Anne R. Diers, Katarzyna A. Broniowska, Ching-Fang Chang, Neil Hogg

Biochem J (2012) 444 (3): 561–571.

DOI: https://doi.org/10.1042/BJ20120294

Published: 29 May 2012

... were allowed to grow for 14 days prior to fixation, staining and scoring of colony formation. α-cyano-4-hydroxycinnamic acid extracellular flux technology mitochondrion reserve capacity Warburg effect Otto Warburg [ 1 ] first described the increased utilization of anaerobic...

View article titled, Pyruvate fuels mitochondrial respiration and proliferation of breast cancer cells: effect of monocarboxylate transporter inhibition

Includes: Supplementary data

Articles

AMPK and GCN2–ATF4 signal the repression of mitochondria in colon cancer cells

Inmaculada Martínez-Reyes, María Sánchez-Aragó, José M. Cuezva

Biochem J (2012) 444 (2): 249–259.

DOI: https://doi.org/10.1042/BJ20111829

Published: 11 May 2012

... at different levels of gene expression, offering the cell a mechanistic safeguard strategy for metabolic adaptation under stressful conditions. ATP synthase cancer cellular stress glycolysis mitochondrion translational control Mitochondria play a central role in the homoeostasis of higher...

View article titled, AMPK and GCN2–ATF4 signal the repression of mitochondria in colon cancer cells

Articles

SIRT3, a pivotal actor in mitochondrial functions: metabolism, cell death and aging

Albert Giralt, Francesc Villarroya

Biochem J (2012) 444 (1): 1–10.

DOI: https://doi.org/10.1042/BJ20120030

Published: 26 April 2012

... is proposed to decline with calorie restriction to permit higher levels of GDH activity [ 30 ] aging mitochondrion oxidative stress SIRT3 sirtuin thermogenesis Sirtuins constitute a family of proteins with NAD + -dependent protein deacetylase and/or ADP-ribosyltransferase activities. Seven...

View article titled, SIRT3, a pivotal actor in mitochondrial functions: metabolism, cell death and aging

Articles

Ribonucleoprotein Y-box-binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA

Shinya Matsumoto, Takeshi Uchiumi, Hiroyuki Tanamachi, Toshiro Saito, Mikako Yagi, Shinya Takazaki, Tomotake Kanki, Dongchon Kang

Biochem J (2012) 443 (2): 573–584.

DOI: https://doi.org/10.1042/BJ20111728

Published: 27 March 2012

... uchiumi@cclm.med.kyushu-u.ac.jp ). 3 10 2011 5 1 2012 27 1 2012 27 1 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 mitochondrion messenger ribonucleoprotein particle (mRNP) oxidative phosphorylation (OXPHOS) translation Y-box-binding protein-1...

View article titled, Ribonucleoprotein Y-box-binding protein-1 regulates mitochondrial oxidative phosphorylation (OXPHOS) protein expression after serum stimulation through binding to OXPHOS mRNA

Includes: Supplementary data

Articles

Membrane integration of a mitochondrial signal-anchored protein does not require additional proteinaceous factors

Elisa Merklinger, Yana Gofman, Alexej Kedrov, Arnold J. M. Driessen, Nir Ben-Tal, Yechiel Shai, Doron Rapaport

Biochem J (2012) 442 (2): 381–389.

DOI: https://doi.org/10.1042/BJ20111363

Published: 13 February 2012

... of this membrane. 1 To whom correspondence should be addressed (email doron.rapaport@uni-tuebingen.de ). 27 7 2011 22 11 2011 5 12 2011 5 12 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 ergosterol mitochondrion outer membrane signal-anchored...

View article titled, Membrane integration of a mitochondrial signal-anchored protein does not require additional proteinaceous factors

Includes: Supplementary data

Articles

Inactivation of mitochondrial aspartate aminotransferase contributes to the respiratory deficit of yeast frataxin-deficient cells

Dominika Sliwa, Julien Dairou, Jean-Michel Camadro, Renata Santos

Biochem J (2012) 441 (3): 945–953.

DOI: https://doi.org/10.1042/BJ20111574

Published: 16 January 2012

... Journal compilation © 2012 Biochemical Society 2012 frataxin Friedreich's ataxia malate–aspartate NADH shuttle mitochondrion protein acetylation yeast FA (Friedreich's ataxia) is the most common autosomal recessive inherited ataxia. It is a slowly progressive neurodegenerative disease...

View article titled, Inactivation of mitochondrial aspartate aminotransferase contributes to the respiratory deficit of yeast frataxin-deficient cells

Articles

Dynamic measurements of mitochondrial hydrogen peroxide concentration and glutathione redox state in rat pancreatic β-cells using ratiometric fluorescent proteins: confounding effects of pH with HyPer but not roGFP1

Leticia P. Roma, Jessica Duprez, Hilton K. Takahashi, Patrick Gilon, Andreas Wiederkehr, Jean-Christophe Jonas

Biochem J (2012) 441 (3): 971–978.

DOI: https://doi.org/10.1042/BJ20111770

Published: 16 January 2012

... compilation © 2012 Biochemical Society 2012 glucose hydrogen peroxide HyPer mitochondrion mt-AlpHi oxidative stress pancreatic β-cell redox-sensitive green fluorescent protein (roGFP) SypHer Glucose stimulation of insulin secretion by pancreatic β-cells plays a critical role in blood...

View article titled, Dynamic measurements of mitochondrial hydrogen peroxide concentration and glutathione redox state in rat pancreatic β-cells using ratiometric fluorescent proteins: confounding effects of pH with HyPer but not roGFP1

Articles

Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling

Jisun Lee, Samantha Giordano, Jianhua Zhang

Biochem J (2012) 441 (2): 523–540.

DOI: https://doi.org/10.1042/BJ20111451

Published: 21 December 2011

...://creativecommons.org/licenses/by-nc/2.5/ ) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. autophagy mitochondrion neurodegeneration nitrative stress oxidative stress redox signalling Autophagy (or self-eating...

View article titled, Autophagy, mitochondria and oxidative stress: cross-talk and redox signalling

Articles

Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signalling

Khandaker Ahtesham Ahmed, Tomohiro Sawa, Hideshi Ihara, Shingo Kasamatsu, Jun Yoshitake, Md. Mizanur Rahaman, Tatsuya Okamoto, Shigemoto Fujii, Takaaki Akaike

Biochem J (2012) 441 (2): 719–730.

DOI: https://doi.org/10.1042/BJ20111130

Published: 21 December 2011

... 6 2011 3 10 2011 4 10 2011 4 10 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 mitochondrion nitric oxide oxidative stress peroxynitrite reactive oxygen species (ROS) reactive oxygen species (ROS) signalling cGMP is a cyclic nucleotide...

View article titled, Regulation by mitochondrial superoxide and NADPH oxidase of cellular formation of nitrated cyclic GMP: potential implications for ROS signalling

Includes: Supplementary data

Articles

LRPPRC mutation suppresses cytochrome oxidase activity by altering mitochondrial RNA transcript stability in a mouse model

Fenghao Xu, Jane B. L. Addis, Jessie M. Cameron, Brian H. Robinson

Biochem J (2012) 441 (1): 275–283.

DOI: https://doi.org/10.1042/BJ20110985

Published: 14 December 2011

... compilation © 2012 Biochemical Society 2012 cytochrome oxidase (COX) gene trap leucine-rich pentatricopeptide repeat-containing (LRPPRC) mitochondrion pentatricopeptide repeat (PPR) respiratory chain Mutations in nuclear genes are widely associated with defects in respiratory chain...

View article titled, LRPPRC mutation suppresses cytochrome oxidase activity by altering mitochondrial RNA transcript stability in a mouse model

Articles

Mutation in the Fe–S scaffold protein Isu bypasses frataxin deletion

Heeyong Yoon, Ramesh Golla, Emmanuel Lesuisse, Jayashree Pain, Jason E. Donald, Elise R. Lyver, Debkumar Pain, Andrew Dancis

Biochem J (2012) 441 (1): 473–480.

DOI: https://doi.org/10.1042/BJ20111637

Published: 14 December 2011

... The Authors Journal compilation © 2012 Biochemical Society 2012 frataxin haem iron iron–sulfur cluster (Fe–S cluster) mitochondrion The open reading frames of NFS1 , ISD11 , ISU1 and ISU2 were amplified by yeast colony PCR from fresh patches of different yeast strains using a 1:1...

View article titled, Mutation in the Fe–S scaffold protein Isu bypasses frataxin deletion

Articles

Aldehyde stress and up-regulation of Nrf2-mediated antioxidant systems accompany functional adaptations in cardiac mitochondria from mice fed n −3 polyunsaturated fatty acids

Ethan J. Anderson, Kathleen Thayne, Mitchel Harris, Kristen Carraway, Saame Raza Shaikh

Biochem J (2012) 441 (1): 359–366.

DOI: https://doi.org/10.1042/BJ20110626

Published: 14 December 2011

... precedes, in chronological order, these mitochondria and redox-mediated adaptations during the course of the diet. aldehyde glutathione lipid peroxidation mitochondrion n −3 polyunsaturated fatty acid permeability transition pore Considerable interest in the relationship between dietary...

View article titled, Aldehyde stress and up-regulation of Nrf2-mediated antioxidant systems accompany functional adaptations in cardiac mitochondria from mice fed n −3 polyunsaturated fatty acids

Includes: Supplementary data

Articles

Respiratory-induced coenzyme Q biosynthesis is regulated by a phosphorylation cycle of Cat5p/Coq7p

Alejandro Martín-Montalvo, Isabel González-Mariscal, Sergio Padilla, Manuel Ballesteros, David L. Brautigan, Plácido Navas, Carlos Santos-Ocaña

Biochem J (2011) 440 (1): 107–114.

DOI: https://doi.org/10.1042/BJ20101422

Published: 27 October 2011

... (Sigma) at 4°C overnight. Proteins were separated by SDS/PAGE (12% gel), and results were visualized using Pro-Q Diamond gel stain (Molecular Probes), autoradiography, silver staining and anti-GST tag immunodetection. coenzyme Q mitochondrion phosphorylation respiration yeast Coenzyme Q...

View article titled, Respiratory-induced coenzyme Q biosynthesis is regulated by a phosphorylation cycle of Cat5p/Coq7p

Includes: Supplementary data

Articles

Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria

Heeyong Yoon, Yan Zhang, Jayashree Pain, Elise R. Lyver, Emmanuel Lesuisse, Debkumar Pain, Andrew Dancis

Biochem J (2011) 440 (1): 137–146.

DOI: https://doi.org/10.1042/BJ20111036

Published: 27 October 2011

.... Supernatant and pellet were separated, and the radioactivity was measured for each fraction. haem iron iron–sulfur mitochondrial carrier protein mitochondrion pyrimidine Iron in cells is used as a cofactor for many essential enzymes [ 1 ]. For example, haem and Fe–S clusters are iron...

View article titled, Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria

Articles

Interaction between the SH3 domain of Src family kinases and the proline-rich motif of HTLV-1 p13: a novel mechanism underlying delivery of Src family kinases to mitochondria

Elena Tibaldi, Andrea Venerando, Francesca Zonta, Carlo Bidoia, Elisa Magrin, Oriano Marin, Antonio Toninello, Luciana Bordin, Veronica Martini, Mario Angelo Pagano, Anna Maria Brunati

Biochem J (2011) 439 (3): 505–518.

DOI: https://doi.org/10.1042/BJ20101650

Published: 13 October 2011

... conditions. human T-cell leukaemia virus type 1 (HTLV-1) p13 mitochondrion Src family kinase (SFK) SH3 domain 1 These authors contributed equally to this work. 2 To whom correspondence should be addressed (email annamaria.brunati@unipd.it ). 7 10 2010 22 6 2011...

View article titled, Interaction between the SH3 domain of Src family kinases and the proline-rich motif of HTLV-1 p13: a novel mechanism underlying delivery of Src family kinases to mitochondria

Includes: Supplementary data

Articles

A critical role for citrate metabolism in LPS signalling

Luke A. J. O'Neill

Biochem J (2011) 438 (3): e5–e6.

DOI: https://doi.org/10.1042/BJ20111386

Published: 26 August 2011

... lipopolysaccharide (LPS) metabolism mitochondrion Toll-like receptor 4 (TLR4) © The Authors Journal compilation © 2011 Biochemical Society 2011 1 email laoneill@tcd.ie 28 7 2011 2 8 2011 As we delve deeper using newer technologies into the metabolic world of a cell activated...

View article titled, A critical role for citrate metabolism in LPS signalling

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