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Keywords: membrane protein
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Biochem J (2016) 473 (7): 851–858.
Published: 29 March 2016
... enzyme adopts a four-transmembrane organization similar to its bacterial homologue. endoplasmic reticulum membrane protein redox-sensitive green fluorescent protein (GFP) thioredoxin (TMX) vitamin K vitamin K epoxide reductase Mammalian vitamin K epoxide reductase (VKOR) is responsible...
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Biochem J (2014) 463 (2): 297–307.
Published: 22 September 2014
.... membrane protein protein purification protein structure Slc26/SulP small-angle neutron scattering transporter The Slc26A proteins are a ubiquitous family of secondary anion transporters conserved from bacteria to man [ 1 ] which belong to the amino acid–polyamine–organocation (APC) superfamily...
Includes: Supplementary data
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Biochem J (2014) 461 (2): 213–222.
Published: 26 June 2014
...Yoel A. Klug; Avraham Ashkenazi; Mathias Viard; Ziv Porat; Robert Blumenthal; Yechiel Shai Lipid-conjugated peptides have advanced the understanding of membrane protein functions and the roles of lipids in the membrane milieu. These lipopeptides modulate various biological systems such as viral...
Includes: Supplementary data
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Biochem J (2014) 461 (2): 269–278.
Published: 26 June 2014
... and are involved in numerous diseases, but study of the structure and function of these proteins is often hampered by their large size and membrane location. Membrane protein purification usually utilizes detergents to solubilize the protein from the membrane, effectively removing it from its native lipid...
Includes: Supplementary data
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Biochem J (2013) 451 (2): 269–275.
Published: 28 March 2013
... 2013 Biochemical Society 2013 DesK histidine kinase lipid regulation membrane protein Cell membranes are composed of a lipid bilayer, containing proteins that either span the bilayer or interact with the lipids on either side of the two leaflets. Membrane proteins come in a huge...
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Biochem J (2012) 442 (3): 631–638.
Published: 24 February 2012
... preparations obtained were analysed by SDS/PAGE (10–20% gel) and immunoblotting using anti- a antibody. a subunit cell-free protein synthesis F 1 F o -ATP synthase (F 1 F o ) H + -pump membrane protein proteoliposome F 1 F o (F 1 F o -ATP synthase) is ubiquitously found...
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Biochem J (2012) 441 (1): 105–112.
Published: 14 December 2011
... endoplasmic reticulum membrane protein N -acetylaspartate is an abundant brain molecule, especially in neurons where its concentration reaches ~20 mM, yet its function is still elusive. Its abundance allows one to determine its concentration in vivo by magnetic resonance spectroscopy. This measure...
Includes: Supplementary data
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Biochem J (2011) 437 (2): e1–e3.
Published: 28 June 2011
... 18065 1 email jh@mrc-mbu.cam.ac.uk 24 5 2011 31 5 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 accessory subunit membrane protein mitochondrion NADH dehydrogenase respiratory chain supernumerary subunit Complex I catalyses...
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Biochem J (2010) 432 (2): 407–416.
Published: 12 November 2010
.... In the present study, we performed detailed characterization of the interaction of 4.1G with various erythroid membrane proteins and the regulation of these interactions by calcium-saturated calmodulin. Like both isoforms of 4.1R, 4.1G bound to band 3, glycophorin C, CD44, p55 and calmodulin. While both 4.1G...
Includes: Supplementary data
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Biochem J (2009) 424 (1): 1–6.
Published: 23 October 2009
...Melanie M. A. Bisson; Andrea Bleckmann; Silke Allekotte; Georg Groth Genetic studies have identified the membrane protein EIN2 (ethylene insensitive 2) as a central component of ethylene signalling in Arabidopsis . In addition, EIN2 might take part in multiple hormone signalling pathways...
Includes: Supplementary data
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Biochem J (2007) 401 (3): 623–633.
Published: 12 January 2007
...Emily R. Slepkov; Jan K. Rainey; Brian D. Sykes; Larry Fliegel The mammalian NHE (Na + /H + exchanger) is a ubiquitously expressed integral membrane protein that regulates intracellular pH by removing a proton in exchange for an extracellular sodium ion. Of the nine known isoforms of the mammalian...
Includes: Supplementary data
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Biochem J (2007) 401 (2): 607–612.
Published: 21 December 2006
..., a single membrane spanning domain, and its C-terminus located in the ER (endoplasmic reticulum) lumen. However, in the present study we show that torsinA is not in fact an integral membrane protein. Instead we find that the mature protein associates peripherally with the ER membrane, most likely through...
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Biochem J (2006) 393 (3): 669–678.
Published: 13 January 2006
.... 1 To whom correspondence should be addressed (email vandervusse@fys.unimaas.nl ). 27 5 2005 4 10 2005 6 10 2005 6 10 2005 The Biochemical Society, London 2006 (5) extracellular matrix fatty acid membrane protein modelling plasma albumin To meet...
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Biochem J (2004) 384 (1): 9–17.
Published: 09 November 2004
... is mediated by its second transmembrane domain, and propose that this interaction may contribute to the recognition of misassembled transmembrane domains during membrane protein quality control at the ER. 1 Present address: European Patent Office, Landsbergerstrasse 30, 80339 München, Germany...
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Biochem J (2004) 378 (3): 1015–1021.
Published: 15 March 2004
... transfected HEK-293 cells or COS7 cells with [ 3 H]palmitic acid failed to produce any detectable AE1 palmitoylation. These results suggest that AE1 is not palmitoylated in HEK-293 or COS7 cells and can traffic to the plasma membrane. anion exchanger 1 (AE1) biosynthesis cysteine membrane protein...
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Biochem J (2003) 372 (2): 329–334.
Published: 01 June 2003
... 2 2003 The Biochemical Society, London ©2003 2003 β-barrel membrane protein pectin degradation Abbreviations used: LB, Luria–Bertani; PEO-MAB; EZ-Link TM PEO maleimide-activated biotin. Biochem. J. (2003) 372, 329 334 (Printed in Great Britain) 329 Topology of the Erwinia...
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Biochem J (2002) 365 (3): 841–848.
Published: 01 August 2002
... of a destabilization of the trimeric form of HA. This temperature is lower than that required for the major endothermic peak observed in DSC experiments. The results demonstrate that there is no kinetic- ally trapped high-energy form of HA at neutral pH. Key words: fusion energy, membrane protein, protein denatur...
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Biochem J (2002) 365 (3): 721–730.
Published: 01 August 2002
... marker lysosomal glycoprotein 120. Although a number of membrane proteins that localize to endosomes/lysosomes contain more than one independently functioning sorting signal, to our knowledge, LAPTM4α is the first example of a membrane protein that requires two tandemly arranged tyrosine-based sorting...
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Biochem J (2002) 364 (3): 777–786.
Published: 15 June 2002
... be addressed (e-mail flintoff@uwo.ca ). 20 9 2001 7 12 2001 9 4 2002 The Biochemical Society, London ©2002 2002 biogenesis intracellular domain membrane protein polytopic topogenesis Abbreviations used: BODIPY ® , 4,4-difluoro-4-bora-3a,4a-diaza- s -indacene; (E)GFP...
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Biochem J (2002) 364 (3): 613–616.
Published: 15 June 2002
... Institutes of Health, 50 South Drive, Bethesda, MD 20892-8030, U.S.A. 2 To whom correspondence should be addressed (e-mail robert.evans@kcl.ac.uk ). 28 3 2002 23 4 2002 25 4 2002 The Biochemical Society, London ©2002 2002 iron membrane protein Neisseria meningitidis...
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Biochem J (2002) 364 (2): 527–535.
Published: 01 June 2002
...Dan BLAKEY; Andrew LEECH; Gavin H. THOMAS; Graham COUTTS; Kim FINDLAY; Mike MERRICK The Amt family of high-affinity ammonium transporters is a family of integral membrane proteins that are found in archaea, bacteria, fungi, plants and animals. Furthermore, the family has recently been extended...
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Biochem J (2001) 359 (3): 707–714.
Published: 25 October 2001
... catalyse triacylglycerol synthesis independently. 1 To whom correspondence should be addressed (e-mail dong.cheng@dupontpharma.com ). 11 6 2001 31 7 2001 5 9 2001 The Biochemical Society, London ©2001 2001 differential splicing membrane protein protein complex...
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Biochem J (2001) 357 (1): 1–10.
Published: 25 June 2001
...Christine A. WIEBE; Emily R. DiBATTISTA; Larry FLIEGEL Na + /H + exchangers are a family of ubiquitous membrane proteins. In higher eukaryotes they regulate cytosolic pH by removing an intracellular H + in exchange for an extracellular Na + . In yeast and Escherichia coli , Na + /H + exchangers...
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Biochem J (2001) 356 (3): 685–704.
Published: 08 June 2001
... at the extracellular surface. J. Biol. Chem. 273, 34190 34195 28 Hu, Y.-K. and Kaplan, J. H. (2000) Site-directed chemical labeling of extracellular loops in a membrane protein. The topology of the Na,K-ATPase a-subunit. J. Biol. Chem. 275, 19185 19191 29 Jorgensen, P. L. (1992) Na,K-ATPase, structure and transport...
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Biochem J (2000) 346 (2): 509–518.
Published: 22 February 2000
..., London © 2000 2000 methotrexate resistance membrane protein N-glycosylation protein degradation Biochem. J. (2000) 346, 509 518 (Printed in Great Britain) 509 Mutations in the reduced-folate carrier affect protein localization and stability Heather SADLISH, Richard C. MURRAY, Frederick M. R...
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Biochem J (1999) 341 (2): 277–284.
Published: 08 July 1999
... the corresponding human protein, a type-I integral membrane protein of 162 amino acids. Madin-Darby canine kidney cells transfected with the cDNA clone directed the cell-surface expression of a 36-kDa O-glycosylated sialoglycoprotein, gp36, and two minor isoforms of 28 and 70 kDa. gp36 has a broad tissue...