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Keywords: lysosome
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Articles
Biochem J (2021) 478 (5): 1159–1173.
Published: 12 March 2021
... reticulum (ER)- Ca 2+ signaling that activates transcriptional factor EB (TFEB), a master transcriptional regulator of autophagic and lysosomal biogenesis. Moreover, RSV activates protein phosphatase 2A (PP2A), which binds and dephosphorylates TFEB, promoting its nuclear translocation and the expression...
Articles
Biochem J (2020) 477 (15): 2841–2857.
Published: 12 August 2020
... metastasis, and diabetes through lysosomal catabolism and desialylation of glycoproteins at the plasma membrane. Various animal models have been established to further explore the functions of vertebrate Neu1. The present study focused on zebrafish ( Danio rerio ) belonging to Cypriniformes...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (8): 1303–1321.
Published: 30 April 2019
... stimulate PGK activity. Localization studies demonstrate that the protein is highly enriched in the glycosome and its presence can also be seen in the lysosome. Gene knockout, overexpression and complement studies suggest that LmPAS-PGK plays a fundamental role in cell survival through autophagy...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (17): 2655–2670.
Published: 30 August 2016
... followed by its translocation into nucleus, and to a lesser extent lysosomes, keeping the net cytosolic amylin content low. An increase in hA accumulation in the nucleus of pancreatic cells correlated with its cytotoxicity, suggesting that its excessive accumulation in the nucleus is detrimental. hA...
Includes: Supplementary data
Articles
Biochem J (2015) 471 (1): 79–88.
Published: 21 September 2015
... ligase that promotes Lys 63 -linked polyubiquitination of MHC class I, providing the signal for clathrin-mediated endocytosis. Endocytosis is followed by sorting into the intralumenal vesicles (ILVs) of multivesicular bodies (MVBs) and eventual delivery to lysosomes. The sorting of MHC class I into MVBs...
Articles
Biochem J (2015) 467 (1): 127–139.
Published: 20 March 2015
... localization of ABCB6 has been a matter of debate, as it has been suggested to reside in the mitochondria and the endo-lysosomal system. Using a variety of imaging modalities, including confocal microscopy and EM, we confirm the endo-lysosomal localization of ABCB6 and show that the protein is internalized...
Articles
Biochem J (2014) 464 (1): 145–156.
Published: 23 October 2014
... phosphorylates two distinct sites in the N-terminus of PI4KIIα (Ser 5 and Ser 47 ), promoting binding to the adaptor protein 3 (AP-3) complex for trafficking to the lysosome to be degraded. Blocking phosphorylation reduces trafficking to the lysosome, stabilizing PI4KIIα and its cargo proteins for redistribution...
Articles
Biochem J (2013) 453 (1): 147–151.
Published: 13 June 2013
... Biochemical Society 2013 calcium endosome lysosome nicotinic acid–adenine dinucleotide phosphate (NAADP) two-pore segment channel 1 (TPCN1) trafficking NAADP (nicotinic acid–adenine dinucleotide phosphate) is a potent and widespread Ca 2+ -mobilizing messenger [ 1 ]. It regulates processes...
Articles
Biochem J (2013) 451 (1): 91–99.
Published: 14 March 2013
... is sufficient to impair all cellular functions of the receptor tested. These findings highlight that the interaction between M6P/IGF2R and M6P-modified ligands is not only important for intracellular accumulation of lysosomal enzymes and formation of dense lysosomes, but is also crucial for the ability...
Includes: Supplementary data
Articles
Biochem J (2013) 449 (2): 449–457.
Published: 14 December 2012
... (endoplasmic reticulum) or through the opening of Ca 2+ -permeable channels in the plasma membrane and influx of extracellular Ca 2+ . Late endocytic pathway compartments including late-endosomes and lysosomes have recently been observed to sequester Ca 2+ to levels comparable with those found within the ER...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2012) 441 (3): 813–821.
Published: 16 January 2012
...; however, the extent of their intracellular uptake is dependent on the characteristics of the NPs. After their uptake by human brain-derived endothelial cells NPs are transported into the lysosomes of these cells, where they enhance the activation of lysosomal proteases. In brain-derived endothelial cells...
Articles
Biochem J (2012) 441 (2): 579–590.
Published: 21 December 2011
... enters the cells prior to cell death. Immunoconfocal and immunogold electron microscopy reveal the path of the Aβ42 with time through the endosomal system and shows that it accumulates in lysosomes. A 24 h incubation with Aβ results in cells that have damaged lysosomes showing signs of enzyme leakage...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (1): 207–216.
Published: 15 March 2011
.../ceroid-like bodies with a lysosomal-type distribution and up-regulate the transcription and translation of proteolytic lysosomal enzymes in cultured J774 mouse macrophages. Given the recently identified role of lysosomes in the induction of apoptosis, we have extended our studies to explore a role...
Includes: Supplementary data
Articles
Biochem J (2011) 434 (2): 219–231.
Published: 11 February 2011
...Jörg Behnke; Eeva-Liisa Eskelinen; Paul Saftig; Bernd Schröder TMEM192 (transmembrane protein 192) is a novel constituent of late endosomal/lysosomal membranes with four potential transmembrane segments and an unknown function that was initially discovered by organellar proteomics. Subsequently...
Articles
Biochem J (2010) 432 (2): 295–301.
Published: 12 November 2010
... 2 O 2 also form and induce oxidative stress with resulting LMP (lysosomal membrane permeabilization) arising from iron-catalysed oxidative events. The latter will contribute significantly to radiation-induced cell death and its degree largely depends on the quantities of lysosomal redox-active iron...
Articles
Biochem J (2010) 430 (2): 305–313.
Published: 13 August 2010
...Marie-Christine Gasingirwa; Jacqueline Thirion; Jeannine Mertens-Strijthagen; Simone Wattiaux-De Coninck; Bruno Flamion; Robert Wattiaux; Michel Jadot It has been suggested that intracellular Hyal-1 (hyaluronidase-1), which is considered a lysosomal enzyme, originates via endocytosis of the serum...
Articles
Biochem J (2010) 428 (3): 355–362.
Published: 27 May 2010
...Su Xu; David E. Sleat; Michel Jadot; Peter Lobel Classical late-infantile neuronal ceroid lipofuscinosis (LINCL) is a fatal neurodegenerative disease of children caused by mutations in TPP1 , the gene encoding the lysosomal protease tripeptidyl peptidase 1. LINCL is characterized by lysosomal...
Includes: Supplementary data
Articles
Biochem J (2010) 428 (2): 183–190.
Published: 13 May 2010
... of H 2 DCF requires the presence of either cytochrome c or of both redox-active transition metals and H 2 O 2 . Redox-active metals exist mainly within lysosomes, whereas cytochrome c resides bound to the outer side of the inner mitochondrial membrane. Following exposure to H 2 DCF-DA, weak...
Articles
Biochem J (2010) 428 (1): 33–45.
Published: 28 April 2010
...Maria R. Rivero; Cecilia V. Vranych; Mariano Bisbal; Belkys A. Maletto; Andrea S. Ropolo; Maria C. Touz The parasite Giardia lamblia possesses PVs (peripheral vacuoles) that function as both endosomes and lysosomes and are implicated in the adaptation, differentiation and survival of the parasite...
Includes: Supplementary data
Articles
Biochem J (2009) 422 (3): 503–512.
Published: 27 August 2009
...Vinita Pandey; Chia-Chen Chuang; Alexander M. Lewis; Parvinder K. Aley; Eugen Brailoiu; Nae J. Dun; Grant C. Churchill; Sandip Patel NAADP (nicotinic acid–adenine dinucleotide phosphate) is an unusual second messenger thought to mobilize acidic Ca 2+ stores, such as lysosomes or lysosome-like...
Articles
Biochem J (2009) 422 (1): 83–90.
Published: 29 July 2009
...Oliver Schieweck; Markus Damme; Bernd Schröder; Andrej Hasilik; Bernhard Schmidt; Torben Lübke Until recently, a modest number of approx. 40 lysosomal membrane proteins had been identified and even fewer were characterized in their function. In a proteomic study, using lysosomal membranes from...
Articles
Biochem J (2009) 417 (1): 133–139.
Published: 12 December 2008
... was confirmed using fluorescence microscopy. Intracellular free zinc was found to be concentrated in lysosomes, indicating that lysosomes are the primary target of zinc ionophores. Furthermore, lysosomal integrity was disrupted after addition of clioquinol and zinc to the cells, as shown by redistribution...
Articles
Biochem J (2008) 414 (3): 431–440.
Published: 27 August 2008
...Marielle Boonen; Roberta Rezende de Castro; Gaëlle Cuvelier; Isabelle Hamer; Michel Jadot Transport of newly synthesized lysosomal membrane proteins from the TGN ( trans -Golgi network) to the lysosomes is due to the presence of specific signals in their cytoplasmic domains that are recognized...
Articles
Biochem J (2008) 410 (2): 417–425.
Published: 12 February 2008
...Silvia Vergarajauregui; Ross Oberdick; Kirill Kiselyov; Rosa Puertollano Mucolipins constitute a family of cation channels with homology with the transient receptor potential family. Mutations in MCOLN1 (mucolipin 1) have been linked to mucolipidosis type IV, a recessive lysosomal storage disease...
Includes: Supplementary data
Articles
Biochem J (2008) 410 (1): 131–140.
Published: 29 January 2008
... dopa incorporation generated proteins that were inefficiently degraded by cells. Incorporation of higher levels of L -dopa into proteins resulted in an increase in the activity of lysosomal cathepsins, increased autofluorescence and the generation of high-molecular-mass SDS-stable complexes, indicative...
Articles
Biochem J (2007) 403 (1): 89–95.
Published: 13 March 2007
...Robert Wattiaux; Simone Wattiaux-De Coninck; Jacqueline Thirion; Mańe-Christine Gasingirwa; Michel Jadot A number of studies, mostly performed ex vivo , suggest that lysosomes are involved in apoptosis as a result of a release of their cathepsins into the cytosol. These enzymes could...
Articles
Biochem J (2006) 395 (1): 39–47.
Published: 15 March 2006
...Marielle Boonen; Isabelle Hamer; Muriel Boussac; Anne-Françoise Delsaute; Bruno Flamion; Jérôme Garin; Michel Jadot Unlike lysosomal soluble proteins, few lysosomal membrane proteins have been identified. Rat liver lysosomes were purified by centrifugation on a Nycodenz density gradient. The most...
Includes: Supplementary data
Articles
Biochem J (2006) 394 (1): 275–283.
Published: 27 January 2006
...Sarah K. Baird; Tino Kurz; Ulf T. Brunk The introduction of apo-ferritin or the iron chelator DFO (desferrioxamine) conjugated to starch into the lysosomal compartment protects cells against oxidative stress, lysosomal rupture and ensuing apoptosis/necrosis by binding intralysosomal redox-active...
Articles
Biochem J (2005) 392 (2): 325–334.
Published: 22 November 2005
...Joseph A. Caruso; Patricia A. Mathieu; John J. Reiners, Jr Recent studies have described a biochemical pathway whereby lysosome disruption and the released proteases initiate the intrinsic apoptotic pathway. Irradiation of murine hepatoma 1c1c7 cells preloaded with the lysosomal photosensitizer...
Articles
Biochem J (2005) 390 (1): 11–18.
Published: 09 August 2005
... of their action remains obscure. Using the confocal spectral imaging technique, we show for the first time that cytotoxins from cobra venom penetrate readily into living cancer cells and accumulate markedly in lysosomes. Cytotoxins CT1 and CT2 from Naja oxiana , CT3 from Naja kaouthia and CT1 from Naja haje...
Articles
Biochem J (2005) 389 (3): 877–884.
Published: 26 July 2005
... in the cytoplasm due to formation of H 2 O 2 may also be important. Since the major pool of low-mass redox-active intracellular iron seems to reside within lysosomes, arising from the continuous intralysosomal autophagocytotic degradation of ferruginous materials, formation of H 2 O 2 inside and outside...
Articles
Biochem J (2005) 387 (3): 703–710.
Published: 26 April 2005
... a rapid, dose-dependent increase in the ICIP (intracellular calcein-chelatable iron pool). Early destabilization of lysosomal membranes and subsequent nuclear DNA strand breaks were also observed, as evaluated by the Acridine Orange relocation test and the comet assay respectively. Somewhat later...
Articles
Biochem J (2004) 381 (2): 537–546.
Published: 06 July 2004
...Gaute HANSEN; Thomas BERG; Hilde M. F. RIISE STENSLAND; Pirkko HEIKINHEIMO; Helle KLENOW; Gry EVJEN; Øivind NILSSEN; Ole K. TOLLERSRUD Human LAMAN (lysosomal α-mannosidase) was synthesized as a 120 kDa precursor in transfected COS cells [African-green-monkey kidney cells], which was partly secreted...
Articles
Biochem J (2004) 378 (3): 1039–1045.
Published: 15 March 2004
...]. Consequently, rupture of secondary lysosomes, as well as subsequent relocation of labile iron to the nucleus, could be an important intermediary step in the generation of oxidative damage to DNA. To test this concept we employed the potent iron chelator DFO (desferrioxamine) conjugated with starch to form...
Articles
Biochem J (2003) 375 (1): 75–86.
Published: 01 October 2003
... used pharmacological inhibitors of protein degradation were first analysed in detail. By choosing specific inhibitors of lysosomes and proteasomes, it was observed that together both pathways accounted for 80% or more of the degradation of cell proteins. With lysosomal inhibitors, it was found...
Articles
Biochem J (2003) 373 (1): 145–153.
Published: 01 July 2003
... interact in endosome compartments. Cells expressing a chimaera of HFE protein with the cytoplasmic domain of lysosomal-associated membrane protein 1 (LAMP1) in place of its own (HFE–LAMP) show a decrease in the half-life of the TfR. This implies that the interaction between HFE and TfR in endosomes targets...
Articles
Biochem J (2003) 371 (2): 429–436.
Published: 15 April 2003
... that 3-AP, having the structure of a weak lysosomotropic base, might concentrate within lysosomes, making these organelles a probable focus of initial toxicity. Indeed, 3-AP leads to lysosomal rupture of D384 glioma cells, a process which clearly precedes caspase activation and apoptotic cell death...
Articles
Biochem J (2003) 370 (1): 185–193.
Published: 15 February 2003
... contributes to the mechanisms of drug resistance. The function of the acidic lysosomes can be altered in MDR cells, and so we investigated the effects of lysosomotropic agents on the secretion of lysosomal enzymes and on the intracellular distribution of the weak-base anthracycline daunomycin in drug...
Articles
Biochem J (2002) 363 (3): 417–429.
Published: 24 April 2002
... endocytosed material and redundant cellular components are hydrolysed. Endocytosed material tends to flow vectorially through the system, proceeding through the early endosome, the endosome carrier vesicle, the late endosome and the lysosome. Phagocytosis and autophagy provide alternative entry points...
Articles
Biochem J (2001) 360 (1): 143–150.
Published: 08 November 2001
... was isolated, confirmed as being overexpressed in septic skeletal muscle and identified as encoding the lysosomal cysteine endopeptidase cathepsin L. Northern- and Western-blot analysis of cathepsin L in gastrocnemius or tibialis anterior muscles of septic rats confirmed an elevation (up to 3-fold) of both...
Articles
Biochem J (2001) 359 (2): 335–343.
Published: 08 October 2001
...Katarina KÅGEDAL; Ming ZHAO; Iréne SVENSSON; Ulf T. BRUNK We propose a new mechanism for sphingosine-induced apoptosis, involving relocation of lysosomal hydrolases to the cytosol. Owing to its lysosomotropic properties, sphingosine, which is also a detergent, especially when protonated...
Articles
Biochem J (2001) 356 (1): 53–60.
Published: 08 May 2001
... in situ hybridization. Ectopic expression of rPHT2 protein in HEK-293T cells and BHK cells was not found on the cell surface, but was found on the lysosomal membrane using light- and electron-microscopic analysis. Recombinant rPHT2 protein reconstituted into liposomes showed proton-dependent transport...
Articles
Biochem J (2001) 353 (3): 655–661.
Published: 25 January 2001
...-kinase (PI-3K), Vps34p, which is required for correct targeting of newly synthesized carboxypeptidase Y to the yeast vacuole. A probable mammalian Vps34p orthologue has been previously identified, but its function in the trafficking of lysosomal enzymes has not been resolved. To investigate the possible...
Articles
Biochem J (2000) 352 (3): 773–781.
Published: 08 December 2000
...Monica FENGSRUD; Camilla RAIBORG; Trond Olav BERG; Per Eivind STRØMHAUG; Takashi UENO; Egil S. ERICHSEN; Per O. SEGLEN In a search for autophagosome-associated proteins, two-dimensional gel separations of proteins from purified autophagosomes, postnuclear supernatant, cytosol, lysosomes...
Articles
Biochem J (2000) 345 (3): 459–466.
Published: 25 January 2000
...Eric OGIER-DENIS; Chantal BAUVY; Françoise CLUZEAUD; Alain VANDEWALLE; Patrice CODOGNO The macroautophagic-lysosomal pathway is a bulk degradative process for cytosolic proteins and organelles including the endoplasmic reticulum (ER). We have previously shown that the human colonic carcinoma HT-29...
Articles
Biochem J (1999) 337 (2): 289–295.
Published: 08 January 1999
...-29 cells. 1 To whom correspondence should be addressed (e-mail codogno@bichat.inserm.fr ). 27 5 1998 8 10 1998 4 11 1998 The Biochemical Society, London © 1999 1999 degradation endoplasmic reticulum GTPase lysosome regulator of G-protein signalling Biochem...