1-25 of 25
Keywords: isothermal titration calorimetry
Close
Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Articles
Biochem J (2022) 479 (16): 1727–1741.
Published: 31 August 2022
... in the mode of oligonucleotide binding by the two Zα domains originating from different proteins. Site-directed mutagenesis was employed together with isothermal titration calorimetry to tease apart finer details of the binding thermodynamics. Our work advances the understanding on binding thermodynamics...
Includes: Supplementary data
Articles
Biochem J (2022) 479 (12): 1303–1315.
Published: 22 June 2022
... domain to antagonize host interferon responses. We examined the NS5 PBM motif interactions with all Scribble PDZ domains using isothermal titration calorimetry, which revealed that the proposed internal PBM did not interact with any Scribble PDZ domains. Instead, the C-terminal PBM of NS5 interacted...
Includes: Supplementary data
Articles
Biochem J (2021) 478 (13): 2715–2732.
Published: 16 July 2021
... ) 1 6 2021 21 6 2021 25 6 2021 25 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 importin isothermal titration calorimetry nuclear localization sequence nucleocytoplasmatic transport X-ray crystallography...
Articles
Biochem J (2021) 478 (5): 1009–1021.
Published: 04 March 2021
... resonance and isothermal titration calorimetry, and to immobilized heparin and heparan sulfate using surface plasmon resonance. These data, in conjunction with molecular docking, indicate that the binding characteristics such as geometry and stoichiometry of the heterodimer are different between free...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (21): 4263–4280.
Published: 13 November 2020
... optimization. Biacore epitope glycosylation isothermal titration calorimetry protein–protein interactions surface plasmon resonance 23 6 2020 13 10 2020 15 10 2020 15 10 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (3): 747–762.
Published: 14 February 2020
... of the C. tropicalis cell wall was damaged. The interaction between CGA-N12 and KRE9 was analyzed by isothermal titration calorimetry (ITC). The results showed that their interaction process was involved an endothermic reaction, and the interaction force was mainly hydrophobic with a few electrostatic...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (10): 1465–1482.
Published: 28 May 2019
... to allow comparison of the UV absorbance at 214 nm. Isothermal titration calorimetry was performed in a semi-automated Affinity ITC LV microcalorimeter (TA Instruments, U.S.A.) as described previously [ 11 ]. Anisotropy-based binding assays were carried out in a Jasco FP-8500 spectrofluorimeter (Jasco Inc...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (9): 1569–1582.
Published: 09 May 2018
... License 4.0 (CC BY) . E3 ligase activity isothermal titration calorimetry Ube2N ubiquitin E3 ligase ZNRF1 ubiquitins Post-translational modification of proteins by conjugation of ubiquitin (Ub) plays crucial roles in almost all subcellular processes in eukaryotes including intracellular...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (21): 3837–3854.
Published: 27 October 2016
...-Ho Lee ( mr0505@protein.osaka-u.ac.jp ) 8 7 2016 15 8 2016 22 8 2016 22 8 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 electron transfer complex enzyme activity ferredoxin isothermal titration calorimetry...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (19): 3321–3339.
Published: 27 September 2016
.... Although CUTr1 and the homeodomain, but not CUTr2, are known to contribute to DNA binding, different research groups have not reached a consensus on which DBD is responsible for recognition of the target sequence in MAR, 5′-TAATA-3′. Here, we used isothermal titration calorimetry to demonstrate that CUTr1...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... © 2016 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2016 CBM32 chitosan crystal structure NMR spectroscopy isothermal titration calorimetry site-directed mutagenesis Interactions between soil bacteria and fungal pathogens play important...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (3): 285–296.
Published: 25 January 2016
... hydrogen–deuterium exchange mass spectrometry isothermal titration calorimetry nuclear magnetic resonance polycystin-2 ADPKD (autosomal dominant polycystic kidney disease) is one of the most common genetic kidney disorders, characterized by the progressive development of renal cysts, ultimately...
Includes: Supplementary data
Articles
Biochem J (2015) 468 (2): 245–257.
Published: 22 May 2015
...-CBM structure, suggesting that it does not form a specific interaction. The orientation of gBCD bound to β1- and β2-CBM is supported by thermodynamic and kinetic data obtained through isothermal titration calorimetry (ITC) and NMR. These results suggest that AMPK containing the muscle-specific β2...
Includes: Supplementary data
Articles
Biochem J (2015) 467 (1): 141–151.
Published: 20 March 2015
...) and isothermal titration calorimetry (ITC). Depending on their locations in the protein, these mutations are found to exert differential effects on the protein stability and target binding. Together with the proposed hinge-and-latch mechanism of Nrf2–Keap1 binding in the literature, our results provide important...
Includes: Supplementary data
Articles
Biochem J (2012) 441 (1): 237–245.
Published: 14 December 2011
... deletion mutants of 4E-BPn ( n =1–3) subtypes were investigated by SPR (surface plasmon resonance) analysis and ITC (isothermal titration calorimetry). Consequently, the conserved PGVTS/T motif within the C-terminal region was shown to act as the second binding region and to play an important role...
Articles
Biochem J (2012) 441 (1): 173–178.
Published: 14 December 2011
... of cognitive disorders, e.g. Alzheimer's disease. These modulators bind within the dimer interface of the LBD (ligand-binding domain) and stabilize the agonist-bound conformation slowing receptor desensitization and/or deactivation. In the present study, we employ isothermal titration calorimetry to determine...
Includes: Supplementary data
Articles
Biochem J (2005) 388 (1): 39–45.
Published: 10 May 2005
...-chloro-1,1,1-trifluoroethane and propofol, to wtHSAd3 and Y411Wd3 was determined using isothermal titration calorimetry, HX and intrinsic tryptophan fluorescence quenching. Although the expressed fragments are less stable than intact wtHSA as indicated by both CD and HX, they retain the secondary...
Articles
Biochem J (2005) 386 (3): 599–605.
Published: 08 March 2005
... ferrochelatase and mesoporphyrin were determined using isothermal titration calorimetry, which revealed a stoichiometry of one molecule of mesoporphyrin bound per protein monomer. The binding is strongly exothermic, with a large intrinsic enthalpy (Δ H =−97.1 kJ · mol −1 ), and is associated with the uptake...
Articles
Biochem J (2005) 385 (1): 145–153.
Published: 14 December 2004
..., determined by isothermal titration calorimetry are similar for zinc binding to E. coli ZiPD ( K d1 =2.2±0.2 μM and K d2 =23.0±0.6 μM) and to the E. coli FlRd metallo-β-lactamase domain ( K d1 =0.7±0.1 μM and K d2 =26.0±0.1 μM). In good correspondence, apo-ZiPD requires incubation with 10 μM zinc for full...
Articles
Biochem J (2004) 382 (2): 703–709.
Published: 24 August 2004
...-1 (human class Alpha GST with two type 1 subunits) and a non-substrate anionic ligand, BSP (bromosulphophthalein), was studied by isothermal titration calorimetry and inhibition kinetics. The binding isotherm is biphasic, best described by a set of two independent sites: a high-affinity site...
Articles
Biochem J (2004) 377 (2): 309–316.
Published: 15 January 2004
... in E. coli GlxI, several approaches were undertaken. Kinetic studies indicate that the catalytic metal ion affects the k cat without significantly affecting the K m for the substrate. Inductively coupled plasma analysis and isothermal titration calorimetry confirmed one metal ion bound to the enzyme...
Articles
Biochem J (2002) 363 (2): 341–346.
Published: 08 April 2002
... GST with two type-1 subunits (hGSTA1-1) and its ΔPhe-222 deletion mutant were studied by isothermal titration calorimetry. The stoichiometry of binding to both proteins is one ANS molecule per GST subunit with a greater affinity for the wild-type ( K d =65 μ M) than for the ΔPhe-222 mutant ( K d =105...
Articles
Biochem J (2002) 361 (1): 27–34.
Published: 17 December 2001
... the non-native polypeptide to Hsp70. 1 To whom correspondence should be addressed (e-mail sha@cmc.uab.edu ). 8 8 2001 1 10 2001 25 10 2001 The Biochemical Society, London ©2002 2002 AFM atomic force microscopy isothermal titration calorimetry ITC protein folding...
Articles
Biochem J (1999) 339 (3): 721–727.
Published: 26 April 1999
... The Biochemical Society, London © 1999 1999 heat capacity isothermal titration calorimetry peptide fragments stopped-flow kinetics Biochem. J. (1999) 339, 721 727 (Printed in Great Britain) 721 Thermodynamic and kinetic analysis of the Escherichia coli thioredoxin-C« fragment complementation system...
Articles
Biochem J (1999) 338 (2): 273–279.
Published: 22 February 1999
... suggests that two lactose molecules bind to one molecule of abrin II at pH 7.2. The presence of two binding sites for lactose on the abrin II molecule is also indicated by isothermal titration calorimetry. Plotting Δ H m (the molar transition enthalpy at T m ) against T m yielded values for Δ C p (change...