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Keywords: isothermal titration calorimetry
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Biochem J (2022) 479 (16): 1727–1741.
Published: 31 August 2022
... in the mode of oligonucleotide binding by the two Zα domains originating from different proteins. Site-directed mutagenesis was employed together with isothermal titration calorimetry to tease apart finer details of the binding thermodynamics. Our work advances the understanding on binding thermodynamics...
Includes: Supplementary data
Articles
Biochem J (2022) 479 (12): 1303–1315.
Published: 22 June 2022
... domain to antagonize host interferon responses. We examined the NS5 PBM motif interactions with all Scribble PDZ domains using isothermal titration calorimetry, which revealed that the proposed internal PBM did not interact with any Scribble PDZ domains. Instead, the C-terminal PBM of NS5 interacted...
Includes: Supplementary data
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Articles
Biochem J (2021) 478 (5): 1009–1021.
Published: 04 March 2021
... resonance and isothermal titration calorimetry, and to immobilized heparin and heparan sulfate using surface plasmon resonance. These data, in conjunction with molecular docking, indicate that the binding characteristics such as geometry and stoichiometry of the heterodimer are different between free...
Includes: Supplementary data
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Articles
Biochem J (2020) 477 (3): 747–762.
Published: 14 February 2020
... of the C. tropicalis cell wall was damaged. The interaction between CGA-N12 and KRE9 was analyzed by isothermal titration calorimetry (ITC). The results showed that their interaction process was involved an endothermic reaction, and the interaction force was mainly hydrophobic with a few electrostatic...
Includes: Supplementary data
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Articles
Biochem J (2018) 475 (9): 1569–1582.
Published: 09 May 2018
... nm. Isothermal titration calorimetries (ITC) were performed on a VP-ITC microcalorimeter (MicroCal, Inc., Northampton, U.S.A.) or AffinityITC LV (TA Instruments, U.S.A.) at 25°C. All protein samples used in ITC experiments were subjected to a final purification step over a Superdex 75 pg 16...
Includes: Supplementary data
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Articles
Biochem J (2016) 473 (19): 3321–3339.
Published: 27 September 2016
.... Although CUTr1 and the homeodomain, but not CUTr2, are known to contribute to DNA binding, different research groups have not reached a consensus on which DBD is responsible for recognition of the target sequence in MAR, 5′-TAATA-3′. Here, we used isothermal titration calorimetry to demonstrate that CUTr1...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... CBM32 chitosan crystal structure NMR spectroscopy isothermal titration calorimetry site-directed mutagenesis Interactions between soil bacteria and fungal pathogens play important roles in sustainably managing agricultural production from soil [ 1 ]. Fungal cell walls contain several kinds...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (3): 285–296.
Published: 25 January 2016
...+ are different. Ca 2+ -binding proteins hydrogen–deuterium exchange mass spectrometry isothermal titration calorimetry nuclear magnetic resonance polycystin-2 ADPKD (autosomal dominant polycystic kidney disease) is one of the most common genetic kidney disorders, characterized...
Includes: Supplementary data
Articles
Biochem J (2015) 468 (2): 245–257.
Published: 22 May 2015
...-CBM structure, suggesting that it does not form a specific interaction. The orientation of gBCD bound to β1- and β2-CBM is supported by thermodynamic and kinetic data obtained through isothermal titration calorimetry (ITC) and NMR. These results suggest that AMPK containing the muscle-specific β2...
Includes: Supplementary data
Articles
Biochem J (2015) 467 (1): 141–151.
Published: 20 March 2015
... understood. To address this problem, we set out to elucidate the effects of disease-associating mis-sense mutations on the structural integrity and target recognition of Keap1 by using NMR spectroscopy, CD spectropolarimetry and isothermal titration calorimetry (ITC). We have chosen to focus on the Kelch...
Includes: Supplementary data
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Articles
Biochem J (2012) 441 (1): 173–178.
Published: 14 December 2011
... of cognitive disorders, e.g. Alzheimer's disease. These modulators bind within the dimer interface of the LBD (ligand-binding domain) and stabilize the agonist-bound conformation slowing receptor desensitization and/or deactivation. In the present study, we employ isothermal titration calorimetry to determine...
Includes: Supplementary data
Articles
Biochem J (2005) 388 (1): 39–45.
Published: 10 May 2005
...-chloro-1,1,1-trifluoroethane and propofol, to wtHSAd3 and Y411Wd3 was determined using isothermal titration calorimetry, HX and intrinsic tryptophan fluorescence quenching. Although the expressed fragments are less stable than intact wtHSA as indicated by both CD and HX, they retain the secondary...
Articles
Biochem J (2005) 386 (3): 599–605.
Published: 08 March 2005
... ferrochelatase and mesoporphyrin were determined using isothermal titration calorimetry, which revealed a stoichiometry of one molecule of mesoporphyrin bound per protein monomer. The binding is strongly exothermic, with a large intrinsic enthalpy (Δ H =−97.1 kJ · mol −1 ), and is associated with the uptake...
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Articles
Biochem J (2004) 382 (2): 703–709.
Published: 24 August 2004
...-1 (human class Alpha GST with two type 1 subunits) and a non-substrate anionic ligand, BSP (bromosulphophthalein), was studied by isothermal titration calorimetry and inhibition kinetics. The binding isotherm is biphasic, best described by a set of two independent sites: a high-affinity site...
Articles
Biochem J (2004) 377 (2): 309–316.
Published: 15 January 2004
... in E. coli GlxI, several approaches were undertaken. Kinetic studies indicate that the catalytic metal ion affects the k cat without significantly affecting the K m for the substrate. Inductively coupled plasma analysis and isothermal titration calorimetry confirmed one metal ion bound to the enzyme...
Articles
Biochem J (2002) 363 (2): 341–346.
Published: 08 April 2002
... GST with two type-1 subunits (hGSTA1-1) and its ΔPhe-222 deletion mutant were studied by isothermal titration calorimetry. The stoichiometry of binding to both proteins is one ANS molecule per GST subunit with a greater affinity for the wild-type ( K d =65 μ M) than for the ΔPhe-222 mutant ( K d =105...
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Articles
Biochem J (1999) 339 (3): 721–727.
Published: 26 April 1999
... The Biochemical Society, London © 1999 1999 heat capacity isothermal titration calorimetry peptide fragments stopped-flow kinetics Biochem. J. (1999) 339, 721 727 (Printed in Great Britain) 721 Thermodynamic and kinetic analysis of the Escherichia coli thioredoxin-C« fragment complementation system...
Articles
Biochem J (1999) 338 (2): 273–279.
Published: 22 February 1999
... suggests that two lactose molecules bind to one molecule of abrin II at pH 7.2. The presence of two binding sites for lactose on the abrin II molecule is also indicated by isothermal titration calorimetry. Plotting Δ H m (the molar transition enthalpy at T m ) against T m yielded values for Δ C p (change...