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Endogenous glutamine is rate-limiting for anti-CD3 and anti-CD28 induced CD4 + T-cell proliferation and glycolytic activity under hypoxia and normoxia

Jonas A. Wik, Azazul Chowdhury, Shrikant Kolan, Nasser E. Bastani, Gaoyang Li, Kazi Alam, Franco Grimolizzi, Bjørn S. Skålhegg

Biochem J (2022) 479 (11): 1221–1235.

DOI: https://doi.org/10.1042/BCJ20220144

Published: 13 June 2022

... and hypoxia (1% O 2 ). This was done in the presence and absence of the GLS and GS inhibitors, Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl) ethyl sulfide (BPTES) and methionine sulfoximine (MSO) and at various combinations of exogenous Gluc, Gln and pyruvate (Pyr) for the last 12 h of stimulation. We found...

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Regulation of chromatin accessibility by hypoxia and HIF

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Towards systems biochemistry

Michael Batie, Julianty Frost, Dilem Shakir, Sonia Rocha

Biochem J (2022) 479 (6): 767–786.

DOI: https://doi.org/10.1042/BCJ20220008

Published: 28 March 2022

...Michael Batie; Julianty Frost; Dilem Shakir; Sonia Rocha Reduced oxygen availability (hypoxia) can act as a signalling cue in physiological processes such as development, but also in pathological conditions such as cancer or ischaemic disease. As such, understanding how cells and organisms respond...

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Systems approaches to understand oxygen sensing: how multi-omics has driven advances in understanding oxygen-based signalling

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Towards systems biochemistry

Michael Batie, Niall S. Kenneth, Sonia Rocha

Biochem J (2022) 479 (3): 245–257.

DOI: https://doi.org/10.1042/BCJ20210554

Published: 04 February 2022

...Michael Batie; Niall S. Kenneth; Sonia Rocha Hypoxia is a common denominator in the pathophysiology of a variety of human disease states. Insight into how cells detect, and respond to low oxygen is crucial to understanding the role of hypoxia in disease. Central to the hypoxic response is rapid...

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Articles

CXCL13 is a differentiation- and hypoxia-induced adipocytokine that exacerbates the inflammatory phenotype of adipocytes through PHLPP1 induction

Joji Kusuyama, Kenjiro Bandow, Tomokazu Ohnishi, Muhammad Subhan Amir, Kaori Shima, Ichiro Semba, Tetsuya Matsuguchi

Biochem J (2019) 476 (22): 3533–3548.

DOI: https://doi.org/10.1042/BCJ20190709

Published: 27 November 2019

...Joji Kusuyama; Kenjiro Bandow; Tomokazu Ohnishi; Muhammad Subhan Amir; Kaori Shima; Ichiro Semba; Tetsuya Matsuguchi Hypoxia in adipose tissue is regarded as a trigger that induces dysregulation of the secretory profile in adipocytes. Similarly, local dysregulation of adipocytokine secretion...

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Articles

Loss of GCN5L1 in cardiac cells disrupts glucose metabolism and promotes cell death via reduced Akt/mTORC2 signaling

Janet R. Manning, Dharendra Thapa, Manling Zhang, Michael W. Stoner, Javier Traba, Catherine Corey, Sruti Shiva, Michael N. Sack, Iain Scott

Biochem J (2019) 476 (12): 1713–1724.

DOI: https://doi.org/10.1042/BCJ20190302

Published: 19 June 2019

.... In this study, we sought to determine the mechanism by which GCN5L1 impacts energy substrate utilization and mitochondrial health. We find that hypoxia and reoxygenation (H/R) leads to a reduction in cell viability and Akt phosphorylation in GCN5L1 knockdown AC16 cardiomyocytes, in parallel with elevated...

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SINHCAF/FAM60A and SIN3A specifically repress HIF-2α expression

John Biddlestone, Michael Batie, Daniel Bandarra, Ivan Munoz, Sonia Rocha

Biochem J (2018) 475 (12): 2073–2090.

DOI: https://doi.org/10.1042/BCJ20170945

Published: 29 June 2018

..., SINHCAF (SIN3A and HDAC-associated factor)/FAM60A (family of homology 60A), links the SIN3A–HDAC co-repressor complex function to the hypoxia response. We show that SINHCAF specifically represses HIF-2α mRNA and protein expression, via its interaction with the transcription factor SP1 (specificity protein...

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Potential mechanisms linking SIRT activity and hypoxic 2-hydroxyglutarate generation: no role for direct enzyme (de)acetylation

Sergiy M. Nadtochiy, Yves T. Wang, Jimmy Zhang, Keith Nehrke, Xenia Schafer, Kevin Welle, Sina Ghaemmaghami, Josh Munger, Paul S. Brookes

Biochem J (2017) 474 (16): 2829–2839.

DOI: https://doi.org/10.1042/BCJ20170389

Published: 10 August 2017

...-HG-generating activity. In vitro acetylation of these enzymes, with confirmation by western blotting, mass spectrometry, reversibility by recombinant sirtuins and an assay for global lysine occupancy, yielded no effect on 2-HG-generating activity. In addition, while elevated 2-HG in hypoxia...

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Post-transcriptional regulation of the tumor suppressor p53 by a novel miR-27a, with implications during hypoxia and tumorigenesis

Raihana Maqbool, Saife Niaz Lone, Mahboob Ul Hussain

Biochem J (2016) 473 (20): 3597–3610.

DOI: https://doi.org/10.1042/BCJ20160359

Published: 11 October 2016

... in the decreased expression of the endogenous p53 protein levels. During hypoxia of the HCT-116 +/+ cells, p53 showed increased accumulation after 3 h, and the levels were significantly up-regulated until 24 h of hypoxia. The p53 expression dynamics during hypoxia of the HCT-116 +/+ cells were found...

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The emerging role of AMPK in the regulation of breathing and oxygen supply

A. Mark Evans, Amira D. Mahmoud, Javier Moral-Sanz, Sandy Hartmann

Biochem J (2016) 473 (17): 2561–2572.

DOI: https://doi.org/10.1042/BCJ20160002

Published: 30 August 2016

... protein kinase (AMPK) apnoea Ca 2+ –calmodulin-activated kinase kinase-β (CaMKK-β) hypoxia liver kinase B1 (LKB1) pulmonary ventilation Regulated oxygen supply is key to the maintenance of oxidative phosphorylation and thus cellular energy status in mammals, not least because of the limited...

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Articles

The Ca 2+ /Mn 2+ -transporting SPCA2 pump is regulated by oxygen and cell density in colon cancer cells

James Jenkins, Dmitri B. Papkovsky, Ruslan I. Dmitriev

Biochem J (2016) 473 (16): 2507–2518.

DOI: https://doi.org/10.1042/BCJ20160477

Published: 11 August 2016

..., is poorly understood. To elucidate the function of SPCA2, we studied human colon cancer HCT116 cells, grown under ambient and decreased O 2 levels. We found that in contrast with other Ca 2+ -ATPase isoforms the expression of SPCA2 was up-regulated under hypoxia (3% O 2 ), in both adherent (2D) and spheroid...

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PAQR3 modulates H3K4 trimethylation by spatial modulation of the regulatory subunits of COMPASS-like complexes in mammalian cells

Chunchun Liu, Yuxue Zhang, Yongfan Hou, Liqiang Shen, Yinlong Li, Weiwei Guo, Daqian Xu, Gaigai Liu, Zilong Zhao, Kaiyang Man, Yi Pan, Zhenzhen Wang, Yan Chen

Biochem J (2015) 467 (3): 415–424.

DOI: https://doi.org/10.1042/BJ20141392

Published: 17 April 2015

.... Hypoxia-induced H3K4me3 was augmented by PAQR3 knockdown and suppressed by PAQR3 overexpression in AGS gastric cancer cells. PAQR3 was able to interact directly or indirectly with the four members of the WRAD sub-complex and tether them to the Golgi apparatus, accompanied by reduction in histone...

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Articles

SIRT3 interactions with FOXO3 acetylation, phosphorylation and ubiquitinylation mediate endothelial cell responses to hypoxia

Anne H.-H. Tseng, Li-Hong Wu, Shyan-Shu Shieh, Danny Ling Wang

Biochem J (2014) 464 (1): 157–168.

DOI: https://doi.org/10.1042/BJ20140213

Published: 23 October 2014

...Anne H.-H. Tseng; Li-Hong Wu; Shyan-Shu Shieh; Danny Ling Wang The endothelial cells (ECs) that line the vascular lumen are exposed to a wide variety of environmental stresses, such as hypoxia. Maladaptation to stress in ECs is a key event in the development of cardiovascular disease. Sirtuin 3...

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Articles

Chromatin and oxygen sensing in the context of JmjC histone demethylases

Alena Shmakova, Michael Batie, Jimena Druker, Sonia Rocha

Biochem J (2014) 462 (3): 385–395.

DOI: https://doi.org/10.1042/BJ20140754

Published: 22 August 2014

... that gene expression is co-ordinated by changes in transcription and translation in hypoxia, much less is known about how chromatin changes allow for transcription to take place. The missing link between co-ordinating chromatin structure and the hypoxia-induced transcriptional programme could be in the form...

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Articles

miR-21 /DDAH1 pathway regulates pulmonary vascular responses to hypoxia

Lucio Iannone, Lan Zhao, Olivier Dubois, Lucie Duluc, Christopher J. Rhodes, John Wharton, Martin R. Wilkins, James Leiper, Beata Wojciak-Stothard

Biochem J (2014) 462 (1): 103–112.

DOI: https://doi.org/10.1042/BJ20140486

Published: 24 July 2014

... of the enzymes metabolizing ADMA, dimethylarginine dimethylaminohydrolases (DDAH1 and DDAH2) and increased levels of miR-21 are linked to disease pathology, but the mechanisms are not understood. In the present study we assessed the potential role of miR-21 in the regulation of hypoxia-induced changes in ADMA...

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Articles

Hypoxia-inducible regulation of placental BOK expression

Daochun Luo, Isabella Caniggia, Martin Post

Biochem J (2014) 461 (3): 391–402.

DOI: https://doi.org/10.1042/BJ20140066

Published: 10 July 2014

...Daochun Luo; Isabella Caniggia; Martin Post BOK (BCL-2-related ovarian killer) is a member of the pro-apoptotic BCL-2 family that is highly expressed in the human placenta. BOK excess causes increased trophoblast autophagy and apoptosis in pre-eclampsia, a pathological condition of hypoxia...

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TIGAR's promiscuity

Juan P. Bolaños

Biochem J (2014) 458 (3): e5–e7.

DOI: https://doi.org/10.1042/BJ20140087

Published: 28 February 2014

... in health and disease. 1 email jbolanos@usal.es 21 1 2014 22 1 2014 © The Authors Journal compilation © 2014 Biochemical Society 2014 2,3-bisphosphoglycerate phosphatase cancer glycolysis haemoglobin hypoxia mitochondrion oxygen sensing TIGAR [TP53 (tumour...

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Articles

Peroxiredoxin-5 targeted to the mitochondrial intermembrane space attenuates hypoxia-induced reactive oxygen species signalling

Simran S. Sabharwal, Gregory B. Waypa, Jeremy D. Marks, Paul T. Schumacker

Biochem J (2013) 456 (3): 337–346.

DOI: https://doi.org/10.1042/BJ20130740

Published: 22 November 2013

...Simran S. Sabharwal; Gregory B. Waypa; Jeremy D. Marks; Paul T. Schumacker The ability to adapt to acute and chronic hypoxia is critical for cellular survival. Two established functional responses to hypoxia include the regulation of gene transcription by HIF (hypoxia-inducible factor...

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Nogo-A couples with Apg-1 through interaction and co-ordinate expression under hypoxic and oxidative stress

Florian Kern, Ruslan I. Stanika, Bettina Sarg, Martin Offterdinger, Daniel Hess, Gerald J. Obermair, Herbert Lindner, Christine E. Bandtlow, Ludger Hengst, Rüdiger Schweigreiter

Biochem J (2013) 455 (2): 217–227.

DOI: https://doi.org/10.1042/BJ20130579

Published: 27 September 2013

... Nogo-A selectively binds to the heat-shock protein Apg-1 and the expression levels of these two interactors are co-regulated under different forms of stress in neurons. heat-shock protein hypoxia interaction neuron Nogo oxidative stress Nogo-A is possibly the best characterized...

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Hypoxia and P1 receptor activation regulate the high-affinity concentrative adenosine transporter CNT2 in differentiated neuronal PC12 cells

Lorena Medina-Pulido, Míriam Molina-Arcas, Carles Justicia, Eduardo Soriano, Ferran Burgaya, Anna M. Planas, Marçal Pastor-Anglada

Biochem J (2013) 454 (3): 437–445.

DOI: https://doi.org/10.1042/BJ20130231

Published: 29 August 2013

...Lorena Medina-Pulido; Míriam Molina-Arcas; Carles Justicia; Eduardo Soriano; Ferran Burgaya; Anna M. Planas; Marçal Pastor-Anglada Under several adverse conditions, such as hypoxia or ischaemia, extracellular levels of adenosine are elevated because of increased energy demands and ATP metabolism...

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Articles

FSL-61 is a 6-nitroquinolone fluorogenic probe for one-electron reductases in hypoxic cells

Jiechuang Su, Christopher P. Guise, William R. Wilson

Biochem J (2013) 452 (1): 79–86.

DOI: https://doi.org/10.1042/BJ20121695

Published: 25 April 2013

...Jiechuang Su; Christopher P. Guise; William R. Wilson One-electron reductases that reduce nitro compounds in hypoxic human tumour cells are poorly characterized, but are important for targeting hypoxia with nitroaromatic prodrugs. Fluorogenic probes with defined reductase profiles are needed...

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Transcriptional up-regulation of ULK1 by ATF4 contributes to cancer cell survival

Luke R. G. Pike, Dean C. Singleton, Francesca Buffa, Olga Abramczyk, Kanchan Phadwal, Ji-Liang Li, Anna Katharina Simon, James T. Murray, Adrian L. Harris

Biochem J (2013) 449 (2): 389–400.

DOI: https://doi.org/10.1042/BJ20120972

Published: 14 December 2012

...Luke R. G. Pike; Dean C. Singleton; Francesca Buffa; Olga Abramczyk; Kanchan Phadwal; Ji-Liang Li; Anna Katharina Simon; James T. Murray; Adrian L. Harris Hypoxia in the microenvironment of many solid tumours is an important determinant of malignant progression. The ISR (integrated stress response...

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p53 Ser 15 phosphorylation disrupts the p53–RPA70 complex and induces RPA70-mediated DNA repair in hypoxia

Esha Madan, Rajan Gogna, Uttam Pati

Biochem J (2012) 443 (3): 811–820.

DOI: https://doi.org/10.1042/BJ20111627

Published: 16 April 2012

... of apoptosis in hypoxic tumours. We have shown that, in cancer cells, hypoxia disrupts the p53–RPA70 complex, thereby enhancing RPA70-mediated NER (nucleotide excision repair)/NHEJ (non-homologous end-joining) repair. In normal cells, RPA70 binds to the p53-NTD (N-terminal domain), whereas this binding...

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Transcriptional regulation mechanisms of hypoxia-induced neuroglobin gene expression

Ning Liu, Zhanyang Yu, Shuanglin Xiang, Song Zhao, Anna Tjärnlund-Wolf, Changhong Xing, Jian Zhang, Xiaoying Wang

Biochem J (2012) 443 (1): 153–164.

DOI: https://doi.org/10.1042/BJ20111856

Published: 14 March 2012

... of hypoxia. In the present study, we located the core proximal promoter of the mouse Ngb gene to a 554 bp segment, which harbours putative conserved NF-κB (nuclear factor κB)- and Egr1 (early growth-response factor 1) -binding sites. Overexpression and knockdown of transcription factors p65, p50, Egr1 or Sp1...

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Hypoxia-inducible factor-1 (HIF-1) promotes LDL and VLDL uptake through inducing VLDLR under hypoxia

Guo-Min Shen, Ying-Ze Zhao, Ming-Tai Chen, Feng-Lin Zhang, Xiao-Ling Liu, Yi Wang, Chang-Zheng Liu, Jia Yu, Jun-Wu Zhang

Biochem J (2012) 441 (2): 675–683.

DOI: https://doi.org/10.1042/BJ20111377

Published: 21 December 2011

...Guo-Min Shen; Ying-Ze Zhao; Ming-Tai Chen; Feng-Lin Zhang; Xiao-Ling Liu; Yi Wang; Chang-Zheng Liu; Jia Yu; Jun-Wu Zhang Metabolism under hypoxia is significantly different from that under normoxia. It has been well elucidated that HIF-1 (hypoxia-inducible factor-1) plays a central role...

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Hypoxia down-regulates sFlt-1 (sVEGFR-1) expression in human microvascular endothelial cells by a mechanism involving mRNA alternative processing

Takayuki Ikeda, Li Sun, Naoki Tsuruoka, Yasuhito Ishigaki, Yasuo Yoshitomi, Yoshino Yoshitake, Hideto Yonekura

Biochem J (2011) 436 (2): 399–407.

DOI: https://doi.org/10.1042/BJ20101490

Published: 13 May 2011

...Takayuki Ikeda; Li Sun; Naoki Tsuruoka; Yasuhito Ishigaki; Yasuo Yoshitomi; Yoshino Yoshitake; Hideto Yonekura sFlt-1 (soluble Flt-1) potently inhibits angiogenesis by binding extracellularly to VEGF (vascular endothelial growth factor). In the present paper, we report that hypoxia down-regulates...

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RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii

James E. Godman, Attila Molnár, David C. Baulcombe, Janneke Balk

Biochem J (2010) 431 (3): 345–352.

DOI: https://doi.org/10.1042/BJ20100932

Published: 11 October 2010

..., whereas Fe-S dependent activities in the chloroplast and mitochondria were unaffected. In addition, the HYD3 -knockdown lines grew poorly on hypoxanthine, indicating impaired function of xanthine dehydrogenase, another cytosolic Fe-S enzyme. The expression levels of selected genes in response to hypoxia...

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Human RASSF7 regulates the microtubule cytoskeleton and is required for spindle formation, Aurora B activation and chromosomal congression during mitosis

Asha Recino, Victoria Sherwood, Amy Flaxman, Wendy N. Cooper, Farida Latif, Andrew Ward, Andrew D. Chalmers

Biochem J (2010) 430 (2): 207–213.

DOI: https://doi.org/10.1042/BJ20100883

Published: 13 August 2010

... of mitosis. In the present study, we address, for the first time, the role of human RASSF7 in mitosis. We demonstrate that RASSF7 is expressed in a broad range of different cell types and that this expression could be enhanced following exposure to hypoxia. Knocking down RASSF7 in human cell lines inhibited...

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Attenuation of the hypoxia-induced protein kinase Cδ interaction with the ‘d’ subunit of F 1 F o -ATP synthase in neonatal cardiac myocytes: implications for energy preservation and survival

Tiffany T. Nguyen, Mourad Ogbi, Qilin Yu, John A. Johnson

Biochem J (2010) 429 (2): 335–345.

DOI: https://doi.org/10.1042/BJ20091927

Published: 28 June 2010

... mitochondrial-targeting sequences. Pre-incubation of NCMs (neonatal cardiac myocyte) with 10 nM extracellular concentrations of the mitochondrial-targeted PKCδ–dF 1 F o interaction inhibitor decreased Hx (hypoxia)-induced co-IP (co-immunoprecipitation) of PKCδ with dF 1 F o by 40±9%, abolished Hx-induced...

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Regulatory factors controlling transcription of Saccharomyces cerevisiae IXR1 by oxygen levels: a model of transcriptional adaptation from aerobiosis to hypoxia implicating ROX1 and IXR1 cross-regulation

Raquel Castro-Prego, Mónica Lamas-Maceiras, Pilar Soengas, Isabel Carneiro, Isabel González-Siso, M. Esperanza Cerdán

Biochem J (2010) 425 (1): 235–243.

DOI: https://doi.org/10.1042/BJ20091500

Published: 14 December 2009

... show that IXR1 mRNA levels are controlled by oxygen availability and increase during hypoxia. In aerobiosis, low levels of IXR1 expression are maintained by Rox1p repression through the general co-repressor complex Tup1–Ssn6. Ixr1p itself is necessary for full IXR1 expression under hypoxic conditions...

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Activating transcription factor 2 increases transactivation and protein stability of hypoxia-inducible factor 1α in hepatocytes

Jeong Hae Choi, Hyun Kook Cho, Yung Hyun Choi, JaeHun Cheong

Biochem J (2009) 424 (2): 285–296.

DOI: https://doi.org/10.1042/BJ20090371

Published: 11 November 2009

...Jeong Hae Choi; Hyun Kook Cho; Yung Hyun Choi; JaeHun Cheong HIF-1 (hypoxia inducible factor 1) performs a crucial role in mediating the response to hypoxia. However, other transcription factors are also capable of regulating hypoxia-induced target-gene transcription. In a previous report, we...

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Articles

AMPKα1 regulates the antioxidant status of vascular endothelial cells

Sergio L. Colombo, Salvador Moncada

Biochem J (2009) 421 (2): 163–169.

DOI: https://doi.org/10.1042/BJ20090613

Published: 26 June 2009

... it [ 1 ]. AMPK is known to be activated under conditions that deplete cellular ATP and elevate AMP levels, such as glucose deprivation, hypoxia, ischaemia and heat shock [ 2 – 4 ]. Previous work, however, indicates that other mechanisms, independent of bioenergetic changes, might be involved in AMPK...

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Role of aryl hydrocarbon receptor in modulation of the expression of the hypoxia marker carbonic anhydrase IX

Martina Takacova, Tereza Holotnakova, Jan Vondracek, Miroslav Machala, Katerina Pencikova, Katarina Gradin, Lorenz Poellinger, Jaromir Pastorek, Silvia Pastorekova, Juraj Kopacek

Biochem J (2009) 419 (2): 419–425.

DOI: https://doi.org/10.1042/BJ20080952

Published: 27 March 2009

...Martina Takacova; Tereza Holotnakova; Jan Vondracek; Miroslav Machala; Katerina Pencikova; Katarina Gradin; Lorenz Poellinger; Jaromir Pastorek; Silvia Pastorekova; Juraj Kopacek Tumour-associated expression of CA IX (carbonic anhydrase IX) is to a major extent regulated by HIF-1 (hypoxia-inducible...

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Isoform-specific differences in the nitrite reductase activity of nitric oxide synthases under hypoxia

Ivan Mikula, Suzanne Durocher, Pavel Martasek, Bulent Mutus, Anny Slama-Schwok

Biochem J (2009) 418 (3): 673–682.

DOI: https://doi.org/10.1042/BJ20080987

Published: 25 February 2009

...Ivan Mikula; Suzanne Durocher; Pavel Martasek; Bulent Mutus; Anny Slama-Schwok Nitrite (NO 2 − ) recycling to nitric oxide (NO) is catalysed by a number of enzymes and induces a protective vasodilation effect under hypoxia/ischaemia. In the present work, we tested the in vitro ability of the three...

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High fat diet induces dysregulation of hepatic oxygen gradients and mitochondrial function in vivo

Sudheer K. Mantena, Denty Paul Vaughn, Jr, Kelly K. Andringa, Heather B. Eccleston, Adrienne L. King, Gary A. Abrams, Jeannette E. Doeller, David W. Kraus, Victor M. Darley-Usmar, Shannon M. Bailey

Biochem J (2009) 417 (1): 183–193.

DOI: https://doi.org/10.1042/BJ20080868

Published: 12 December 2008

... respectively, for 8 or 16 weeks and liver hypoxia, mitochondrial bioenergetics, NO (nitric oxide)-dependent control of respiration, and 3-NT (3-nitrotyrosine), a marker of protein modification by RNS, were examined. Feeding a HFD for 16 weeks induced NASH-like pathology accompanied by elevated triacylglycerols...

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Regulation of Jumonji-domain-containing histone demethylases by hypoxia-inducible factor (HIF)-1α

Patrick J. Pollard, Christoph Loenarz, David R. Mole, Michael A. McDonough, Jonathan M. Gleadle, Christopher J. Schofield, Peter J. Ratcliffe

Biochem J (2008) 416 (3): 387–394.

DOI: https://doi.org/10.1042/BJ20081238

Published: 26 November 2008

...Patrick J. Pollard; Christoph Loenarz; David R. Mole; Michael A. McDonough; Jonathan M. Gleadle; Christopher J. Schofield; Peter J. Ratcliffe The transcription factor HIF (hypoxia-inducible factor) mediates a highly pleiotrophic response to hypoxia. Many recent studies have focused on defining...

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Regulation of gene expression by hypoxia

Niall Steven Kenneth, Sonia Rocha

Biochem J (2008) 414 (1): 19–29.

DOI: https://doi.org/10.1042/BJ20081055

Published: 29 July 2008

...Niall Steven Kenneth; Sonia Rocha Hypoxia induces profound changes in the cellular gene expression profile. The discovery of a major transcription factor family activated by hypoxia, HIF (hypoxia-inducible factor), and the factors that contribute to HIF regulation have greatly enhanced our...

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Cathepsin L expression is up-regulated by hypoxia in human melanoma cells: role of its 5′-untranslated region

Didier Jean, Nathalie Rousselet, Raymond Frade

Biochem J (2008) 413 (1): 125–134.

DOI: https://doi.org/10.1042/BJ20071255

Published: 12 June 2008

... that the cathepsin L 5′-UTR contained a functional IRES (internal ribosome entry site). This complete IRES was present only in one of the three splice variants, which differed in their 5′-UTR. Then, we analysed cathepsin L expression in this human melanoma cell line grown under hypoxia. We demonstrated that under...

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Regulation of hypoxia-inducible factor-1α by NF-κB

Patrick van Uden, Niall S. Kenneth, Sonia Rocha

Biochem J (2008) 412 (3): 477–484.

DOI: https://doi.org/10.1042/BJ20080476

Published: 28 May 2008

...Patrick van Uden; Niall S. Kenneth; Sonia Rocha HIF (hypoxia-inducible factor) is the main transcription factor activated by low oxygen tensions. HIF-1α (and other α subunits) is tightly controlled mostly at the protein level, through the concerted action of a class of enzymes called PHDs (prolyl...

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Annexins sense changes in intracellular pH during hypoxia

Katia Monastyrskaya, Fabian Tschumi, Eduard B. Babiychuk, Deborah Stroka, Annette Draeger

Biochem J (2008) 409 (1): 65–75.

DOI: https://doi.org/10.1042/BJ20071116

Published: 11 December 2007

...Katia Monastyrskaya; Fabian Tschumi; Eduard B. Babiychuk; Deborah Stroka; Annette Draeger The pH i (intracellular pH) is an important physiological parameter which is altered during hypoxia and ischaemia, pathological conditions accompanied by a dramatic decrease in pH i . Sensors of pH i include...

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Mitochondria and cellular oxygen sensing in the HIF pathway

Cormac T. Taylor

Biochem J (2008) 409 (1): 19–26.

DOI: https://doi.org/10.1042/BJ20071249

Published: 11 December 2007

... levels to the point where oxygen demand exceeds supply (termed hypoxia) leads rapidly to metabolic crisis and represents a severe threat to ongoing physiological function and ultimately, viability. Because of the central role of oxygen in metabolism, it is perhaps not surprising that we have evolved...

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Hypoxia, via stabilization of the hypoxia-inducible factor HIF-1α, is a direct and sufficient stimulus for brain-type natriuretic peptide induction

Alexander Weidemann, Bernd Klanke, Michael Wagner, Tilmann Volk, Carsten Willam, Michael S. Wiesener, Kai-Uwe Eckardt, Christina Warnecke

Biochem J (2008) 409 (1): 233–242.

DOI: https://doi.org/10.1042/BJ20070629

Published: 11 December 2007

.... It is induced under a variety of pathophysiological conditions, including decompensated heart failure and myocardial infarction. Since regional hypoxia is a potential common denominator of increased wall stretch and myocardial hypoperfusion, we investigated the direct effects of hypoxia on BNP expression...

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Enzyme substrate recognition in oxygen sensing: how the HIF trap snaps

Eric Metzen

Biochem J (2007) 408 (2): e5–e6.

DOI: https://doi.org/10.1042/BJ20071306

Published: 14 November 2007

...Eric Metzen The transcriptional activator HIF (hypoxia-inducible factor) is a focal point of biomedical research because many situations in physiology and in pathology coincide with hypoxia. The effects of HIF activation may be a facet of normal growth, as in embryonic development, they may...

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Identification of a region on hypoxia-inducible-factor prolyl 4-hydroxylases that determines their specificity for the oxygen degradation domains

Diego Villar, Alicia Vara-Vega, Manuel O. Landázuri, Luis Del Peso

Biochem J (2007) 408 (2): 231–240.

DOI: https://doi.org/10.1042/BJ20071052

Published: 14 November 2007

...Diego Villar; Alicia Vara-Vega; Manuel O. Landázuri; Luis Del Peso HIFs [hypoxia-inducible (transcription) factors] are essential for the induction of an adaptive gene expression programme under low oxygen partial pressure. The activity of these transcription factors is mainly determined...

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Hypoxia-induced assembly of prolyl hydroxylase PHD3 into complexes: implications for its activity and susceptibility for degradation by the E3 ligase Siah2

Koh Nakayama, Stefan Gazdoiu, Robert Abraham, Zhen-Qiang Pan, Ze'ev Ronai

Biochem J (2007) 401 (1): 217–226.

DOI: https://doi.org/10.1042/BJ20061135

Published: 11 December 2006

...Koh Nakayama; Stefan Gazdoiu; Robert Abraham; Zhen-Qiang Pan; Ze'ev Ronai PHD1–3 (prolyl hydroxylases 1–3) catalyse the hydroxylation of HIF (hypoxia-inducible factor)-α subunit that triggers the substrate ubiquitination and subsequent degradation. The RING (really interesting new gene) finger E3...

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Articles

Analysis of the hypoxia-sensing pathway in Drosophila melanogaster

Nathalie Arquier, Paul Vigne, Eric Duplan, Tien Hsu, Pascal P. Therond, Christian Frelin, Gisela D'Angelo

Biochem J (2006) 393 (2): 471–480.

DOI: https://doi.org/10.1042/BJ20050675

Published: 23 December 2005

...Nathalie Arquier; Paul Vigne; Eric Duplan; Tien Hsu; Pascal P. Therond; Christian Frelin; Gisela D'Angelo The mechanism by which hypoxia induces gene transcription involves the inhibition of HIF-1α (hypoxia-inducible factor-1 α subunit) PHD (prolyl hydroxylase) activity, which prevents the VHL (von...

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Articles

A VEGF/JAK2/STAT5 axis may partially mediate endothelial cell tolerance to hypoxia

Andrew C. Dudley, David Thomas, James Best, Alicia Jenkins

Biochem J (2005) 390 (2): 427–436.

DOI: https://doi.org/10.1042/BJ20050351

Published: 23 August 2005

...). These results indicate that the JAK/STAT pathway may play a pivotal role during tumour-associated or retinal angiogenesis in which endothelial cell survival during tissue hypoxia is critical for maintaining either the growth of neoplasms or the inappropriate retinal neovascularization common in diabetic...

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Articles

Regulation of the prolyl hydroxylase domain protein 2 ( phd2 / egln-1 ) gene: identification of a functional hypoxia-responsive element

Eric METZEN, Daniel P. STIEHL, Kathrin DOEGE, Jan H. MARXSEN, Thomas HELLWIG-BÜRGEL, Wolfgang JELKMANN

Biochem J (2005) 387 (3): 711–717.

DOI: https://doi.org/10.1042/BJ20041736

Published: 26 April 2005

...Eric METZEN; Daniel P. STIEHL; Kathrin DOEGE; Jan H. MARXSEN; Thomas HELLWIG-BÜRGEL; Wolfgang JELKMANN The HIFs (hypoxia-inducible factors) are a family of heterodimeric transcription factors essential for the adaptation of cells to reduced oxygen supply. Three human PHDs (prolyl hydroxylase domain...

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Replication of simian virus 40 (SV40) DNA in virus-infected CV1 cells selectively permeabilized for small molecules by Staphylococcus aureus α-toxin: involvement of mitochondria in the fast O 2 -dependent regulation of SV40 DNA replication

Hans-Jörg RIEDINGER, Frank EGER, Klaus TRUMMLER, Hans PROBST

Biochem J (2005) 386 (3): 557–566.

DOI: https://doi.org/10.1042/BJ20040492

Published: 08 March 2005

... conditions caused inhibition of SV40 DNA replication. As seen in unpermeabilized cells, SV40 DNA replication was inhibited at the stage of initiation. The inhibition of DNA replication induced by hypoxia was mimicked by AA (antimycin A), an inhibitor of mitochondrial respiration, and also by the replacement...

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Disruption of dimerization and substrate phosphorylation inhibit factor inhibiting hypoxia-inducible factor (FIH) activity

David E. LANCASTER, Luke A. McNEILL, Michael A. McDONOUGH, Robin T. APLIN, Kirsty S. HEWITSON, Christopher W. PUGH, Peter J. RATCLIFFE, Christopher J. SCHOFIELD

Biochem J (2004) 383 (3): 429–437.

DOI: https://doi.org/10.1042/BJ20040735

Published: 26 October 2004

...David E. LANCASTER; Luke A. McNEILL; Michael A. McDONOUGH; Robin T. APLIN; Kirsty S. HEWITSON; Christopher W. PUGH; Peter J. RATCLIFFE; Christopher J. SCHOFIELD HIF (hypoxia-inducible factor) is an αβ transcription factor that modulates the hypoxic response in many animals. The cellular abundance...

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Includes: Supplementary data

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Endogenous 2-oxoacids differentially regulate expression of oxygen sensors

Clifton Lee DALGARD, Huasheng LU, Ahmed MOHYELDIN, Ajay VERMA

Biochem J (2004) 380 (2): 419–424.

DOI: https://doi.org/10.1042/bj20031647

Published: 01 June 2004

... factors HIFs (hypoxia-inducible factors). Enzymes that link changes in oxygen tensions with the stability and transcriptional activity of HIFs are considered as oxygen sensors. These enzymes are oxygen-, iron- and 2-oxoglutarate-dependent dioxygenases that hydroxylate key proline and asparagine residues...

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