... on behalf of the Biochemical Society 2016 collagen recognition collagen stability collagen structure hydrogen bonding protein design and engineering In its most widely used meaning, the term ‘collagen’ refers to the main structural protein in connective tissues such as skin, bone, tendons...

... protonation states and amide temperature coefficients were used to determine the orientation of the histidine ring and hydrogen-bonding arrangements within the HPN motif. Unlike many three-dimensional structures, we found that a conserved hydrogen bond between the histidine imidazole ring and the asparagine...

... compounds. In the present study, the effect of metal ion substitutions and site-directed mutations on the catalytic properties of OpdA are investigated. The study shows the importance of both the metal ion composition and a hydrogen-bond network that connects the metal ion centre with the substrate-binding...

... reductase (AKR) Candida tenuis xylose reductase hydrogen bonding ketone reduction structure–activity correlation substrate selectivity The AKRs (aldo–keto reductases) constitute a large protein superfamily of mainly NAD(P)H-dependent reductases that occur in a wide variety of organisms...

... as an active-site residue and implicated a putative role for this residue in substrate enantioselectivity and the differential inhibition of enzyme activity by the diastereoisomers quinine and quinidine. The role of serine 304 in selectivity is thought to be achieved through a preferential hydrogen-bond...