... in vitro and in vivo and as platforms to screen for anti-HIV-1 drugs. The first replication-competent HIV-1 vector was constructed by insertion of the CAT (chloramphenicol acetyltransferase) gene in the place of nef [ 20 ]. cell targeting central polypurine tract (cPPT) gene therapy HIV-1...

... The Authors Journal compilation © 2012 Biochemical Society 2012 gene therapy hypoxia nucleotide excision repair (NER) non-homologous end-joining (NHEJ) p53 replication protein A 70 kDa subunit (RPA70) RPA (replication protein A), the eukaryote ssDNA (single-stranded DNA)-binding protein...

... hope for patients, but has also provoked discussion about traditional boundaries between neurodevelopmental disorders and those involving dysfunction at later stages. In the present paper we review the neurobiology of MeCP2 and consider the various genetic (including gene therapy), pharmacological...

..., a complement regulatory protein. Such species-specific differences, as well as adaptations or modifications of Ads required for applications in gene therapy, form the major focus of the present review. 1 To whom correspondence should be addressed (email g.e.blair@leeds.ac.uk ). 24 5 2010 26...

...Kylie M. Wagstaff; David A. Jans Gene therapy, the correction of dysfunctional or deleted genes by supplying the lacking component, has long been awaited as a means to permanently treat or reverse many genetic disorders. To achieve this, therapeutic DNA must be delivered to the nucleus of cells...

... in lung was capable of decreasing the GAG level by 29%. Long-term (6 months) follow up of the gene therapy revealed that the viral genome integration persisted in the target tissues, although the real-time PCR analysis showed a decrease in the vector DNA content with time. Interestingly, the decrease...

... than that in wild-type mice. These results demonstrate that even moderate APOBEC-1 expression can lead to hyperediting, limiting the single-gene approach for gene therapy with APOBEC-1. Biochem. J. (2003) 369, 255 262 (Printed in Great Britain) 255 Regulatable liver expression of the rabbit...

... 2002 The Biochemical Society, London ©2002 2002 antisense gene therapy hexagonal phase lipoplex Abbreviations used: Cer-C 8 (etc.), ceramide with a C 8 fatty acyl chain length (etc.); CHO, Chinese hamster ovary; CRF-R, corticotropin-releasing factor receptor; DOPE, dioleoyl...

... of these novel macrophage-tropic DNA enzymes are discussed. 2 1 2001 14 3 2001 4 4 2001 The Biochemical Society, London ©2001 2001 1 To whom correspondence should be addressed (e-mail akhil@nii.res.in ). gene silencing gene therapy HIV-1 replication Abbreviations...

...Barbara WETZER; Gerardo BYK; Marc FREDERIC; Marc AIRIAU; Francis BLANCHE; Bruno PITARD; Daniel SCHERMAN One of the main challenges of gene therapy remains the increase of gene delivery into eukaryotic cells. We tested whether intracellular DNA release, an essential step for gene transfer, could...

... be addressed (e-mail akhil@nii.res.in ). 4 9 2000 16 10 2000 16 11 2000 The Biochemical Society, London © 2001 2001 gene cleavage gene silencing gene therapy Biochem. J. (2001) 353, 701 708 (Printed in Great Britain) 701 Inhibition of hepatitis B virus X gene expression...

... of exogenous sulphated glycosaminoglycans. We conclude that the glycosaminoglycan part of proteoglycans serves a protective role against cationic lipid cytotoxicity, allowing optimal transfection efficiency in Šitro. Key words: gene therapy, glycosaminoglycan, liposomes, polyamines. It has been suggested...

... for a regulatory role of apoC1 in the delivery of lipoprotein constituents to different tissues on which these receptors are located. Key words: apolipoprotein C, gene therapy, lipoproteins, recep- tors. Thus from these studies in Šitro it can be concluded that apoC1 inhibits the uptake of TG-rich lipoproteins...