... potential, and low numbers. The key transcription regulators of mitochondrial biogenesis also decreased. These results indicate that mitochondrial dysfunction leads to an inability to support the pluripotency maintenance. Moreover, down-regulated glutathione metabolism and up-regulated focal adhesion...

... adhesion, a critical step in the second phase of insulin secretion, we examined the effect of silencing SCGN on focal adhesion molecules, including FAK (focal adhesion kinase) and paxillin, and the cell survival molecules ERK1/2 (extracellular-signal-regulated kinase 1/2) and Akt. We found that glucose...

... the vinculin tail:actin interface proposed by Janssen. These studies reveal regions important for actin binding and bundling located outside of the proposed Janssen sites (I997, V1001; [ 15 , 17 ]). In support of this, full-length vinculin harbouring either I997A or V1001A mutations compromise focal adhesion...

... cell attachment to fibronectin recruits MEKK2 to focal adhesion complexes, and that MEKK2 knockdown is associated with stabilized focal adhesions and significant inhibition of tumour cell migration. In the present study we investigate MEKK2 function in focal adhesions and we report that MEKK2...

.... The foregoing discussion proposed that AC scaffolds are organized in their cellular destination as part of a regulated, but continuous, process. A more dynamic situation may occur at FAs (focal adhesions), where a scaffolding AC could play a key regulatory and dynamic structural role. FAs are large...

...Kentaro Kajiwara; Takayuki Yamada; Takeshi Bamba; Eiichiro Fukusaki; Fumio Imamoto; Masato Okada; Chitose Oneyama The proto-oncogenic tyrosine kinase c-Src is up-regulated in various human cancers, implicating its role in tumour progression. Upon activation, c-Src translocates to focal adhesions...

... as with focal adhesions and cell junctions, and are probably involved in cell migration, polarity and proliferation. Finally, we consider the association of GCKIIIs with a number of human diseases, particularly cerebral cavernous malformations. Rather than requiring a distinct upstream kinase, GCKIIIs...

... in controlling cell spreading and migration. cell division cycle 42 (Cdc42) focal adhesion mRNA Rac1 RhoA translation Fibroblasts are highly migratory mesenchymal cells that mediate formation and remodelling of epithelial tissues and wound healing. As such, fibroblasts are key determinants...

... investigate the effect of the electrophilic prostaglandin 15d-PGJ 2 (15-deoxy-Δ 12,14 -prostaglandin J 2 ) on metastatic properties of breast cancer cells. 15d-PGJ 2 was shown to decrease migration, stimulate focal-adhesion disassembly and cause extensive F-actin (filamentous actin) reorganization at low...

... of the type IV intermediate filament protein synemin interacts directly with the focal adhesion protein vinculin in its active state. Interaction of adhesion components with intermediate filaments could serve as a general mechanism to regulate cell- and tissue-specific cytoskeleton-membrane attachment...

... with vinculin in vivo , and this association is promoted by PtdIns(4,5) P 2 . SNTIII also specifically co-localizes with vinculin within focal adhesions when transiently expressed in mammalian cells. In contrast, other regions of synemin show distinct localization patterns in comparison with those of SNTIII...

...: the potential tumour suppressor Tes, an actin-binding protein mainly located at focal adhesions; and EVL (Ena/vasodilator-stimulated phosphoprotein-like protein), another actin-binding protein, equally recruited at focal adhesions. Interactions between spectrin and overexpressed Tes and EVL were confirmed by co...

... 12 12 2003 The Biochemical Society, London ©2004 2004 α-actinin actin bundling cytoskeleton focal adhesion phosphoinositide protein flexibility Abbreviations used: CH, calponin homology; GST, glutathione S-transferase. Biochem. J. (2004) 378, 1067 1072 (Printed in Great...

... plays a role in skeletal-muscle regeneration. 1 To whom correspondence should be addressed (e-mail shlo@ucdavis.edu ). 2 10 2000 12 3 2001 5 4 2001 The Biochemical Society, London ©2001 2001 actin binding cardiotoxin focal adhesion SH2 domain Abbreviations...

...Huaiyang CHEN; Akiko ISHII; Wai-Keung WONG; Lan Bo CHEN; Su Hao LO Tensin is a focal-adhesion molecule that binds to actin filaments and interacts with phosphotyrosine-containing proteins. To analyse tensin's function in mammals, we have cloned tensin cDNAs from human and cow. The isolated approx...