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Keywords: exocytosis
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Articles
Biochem J (2017) 474 (19): 3339–3354.
Published: 25 September 2017
... in reconstituted exocytic reactions mediated by neuronal and non-neuronal SNAREs. We show that Munc18a plays a direct role in promoting proteoliposome clustering, underlying vesicle docking during exocytosis. In the three different fusion reactions examined, Munc18a-dependent clustering requires an intact N...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (12): 2039–2049.
Published: 06 June 2017
... concurrent interaction with PS in trans through its amphipathic N-terminal domain facilitate SNARE complex formation, whereby A-syn aids SNARE-dependent vesicle docking. Recently, an important discovery has been made, which might provide some clues to the function of A-syn in exocytosis: A-syn interacts...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (5): 627–639.
Published: 24 February 2016
...Michael C. Chicka; Qiansheng Ren; David Richards; Lance M. Hellman; Jinchao Zhang; Michael G. Fried; Sidney W. Whiteheart The Munc13 family of exocytosis regulators has multiple Ca 2+ -binding, C2 domains. Here, we probed the mechanism by which Munc13-4 regulates in vitro membrane fusion...
Articles
Biochem J (2015) 471 (3): 381–389.
Published: 16 October 2015
... of adiponectin. Taken together, our results show that adiponectin is stored in a unique vesicular compartment, and released through a regulated exocytosis pathway that is dependent on insulin signalling. 1 To whom correspondence should be addressed (email weiping_han@sbic.a-star.edu.sg ). 10 3...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2015) 471 (1): 111–122.
Published: 21 September 2015
... are not driven by upstream metabolic fluctuations, but rather by autonomous ionic mechanisms, the details of which are unclear. We have investigated the nature of the oscillator, with simultaneous fluorescence monitoring of Δψ p , [Ca 2+ ] c and exocytosis at single-cell resolution, combined with analysis...
Articles
Biochem J (2015) 465 (3): 359–370.
Published: 22 January 2015
...Claudia Nora Tomes Exocytosis is a highly regulated process that consists of multiple functionally, kinetically and/or morphologically definable stages such as recruitment, targeting, tethering and docking of secretory vesicles with the plasma membrane, priming of the fusion machinery and calcium...
Articles
Biochem J (2013) 450 (2): 365–373.
Published: 15 February 2013
...Tetsuya Kitaguchi; Manami Oya; Yoshiko Wada; Takashi Tsuboi; Atsushi Miyawaki Intracellular cAMP and Ca 2+ are important second messengers that regulate insulin secretion in pancreatic β-cells; however, the molecular mechanism underlying their mutual interaction for exocytosis is not fully...
Includes: Supplementary data
Articles
Biochem J (2012) 446 (3): 383–394.
Published: 28 August 2012
..., distribution and reproduction in any medium, provided the original work is properly cited. exocytosis mast cell mucin mucus Munc18 secretion SNARE [SNAP (soluble N -ethylmaleimide-sensitive factor-attachment protein) receptor] proteins and SM (Sec1/Munc18) proteins collaborate to mediate...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (1): 59–67.
Published: 26 April 2012
... ability to reduce exocytosis from non-neuronal cells expressing the BoNT/A-acceptor and utilising VAMP, but not SNAP-25, in exocytosis. 1 To whom correspondence should be addressed (email oliver.dolly@dcu.ie ). 16 1 2012 22 2 2012 23 2 2012 23 2 2012 © The Authors...
Articles
Biochem J (2010) 432 (2): 375–386.
Published: 12 November 2010
... remains unclear. In the present study, we investigated the physiological role of insulin and PI3K in glucose-induced biphasic insulin exocytosis in primary cultured β-cells and insulinoma Min6 cells using total internal reflection fluorescent microscopy. The pretreatment of β-cells with insulin induced...
Articles
Biochem J (2010) 429 (3): 497–504.
Published: 14 July 2010
...Ryo Aoki; Tetsuya Kitaguchi; Manami Oya; Yu Yanagihara; Mai Sato; Atsushi Miyawaki; Takashi Tsuboi Since the fusion pore of the secretory vesicle is resealed before complete dilation during ‘kiss-and-run’ exocytosis, their cargoes are not completely released. Although the transient fusion pore...
Includes: Supplementary data
Articles
Biochem J (2010) 426 (1): 55–64.
Published: 27 January 2010
... was shown to contribute to several synapsin functions, ranging from formation of the SV reserve pool to regulation of the kinetics of exocytosis and SV cycling, although the molecular mechanisms underlying these effects are unknown. In the present study, we used a synthetic 25-mer peptide encompassing...
Articles
Biochem J (2009) 423 (1): 1–14.
Published: 14 September 2009
...Matthew A. Churchward; Jens R. Coorssen Exocytosis is a highly conserved and essential process. Although numerous proteins are involved throughout the exocytotic process, the defining membrane fusion step appears to occur through a lipid-dominated mechanism. Here we review and integrate the current...
Articles
Biochem J (2008) 413 (1): 71–80.
Published: 12 June 2008
... of Kv4.2 channels to the plasma membrane. KChIP3 expression in PC12 cells resulted in an increase in exocytosis evoked by activation of purinergic receptors. In contrast, KChIPs 1, 2 and 4, although expressed to the same extent, had no effect on secretion. In addition, KChIP3 but not KChIPs 1, 2 and 4...
Articles
Biochem J (2008) 412 (1): 93–101.
Published: 25 April 2008
... release occurred with glibenclamide treatment. In accord with the perifusion results, TIRF (total internal reflection fluorescence) imaging of insulin exocytosis showed restoration of the decreased number of docked insulin granules and the fusion events from them during first-phase release for nateglinide...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2008) 409 (2): 407–416.
Published: 21 December 2007
...Margaret E. Graham; Mark T. W. Handley; Jeff W. Barclay; Leo F. Ciufo; Stephanie L. Barrow; Alan Morgan; Robert D. Burgoyne Munc18-1 plays a crucial role in regulated exocytosis in neurons and neuroendocrine cells through modulation of vesicle docking and membrane fusion. The molecular basis...
Articles
Biochem J (2007) 404 (3): 525–534.
Published: 29 May 2007
... contributed equally to this work. 2 To whom correspondence should be addressed (email valtorta.flavia@hsr.it ). 20 12 2006 28 2 2007 2 3 2007 2 3 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 endocytosis exocytosis synaptobrevin 2...
Includes: Supplementary data
Articles
Biochem J (2007) 403 (3): 483–492.
Published: 12 April 2007
...Florence Grise; Nada Taib; Carole Monterrat; Valérie Lagrée; Jochen Lang Synaptotagmins form a family of calcium-sensor proteins implicated in exocytosis, and these vesicular transmembrane proteins are endowed with two cytosolic calcium-binding C 2 domains, C 2 A and C 2 B. Whereas the isoforms...
Articles
Biochem J (2007) 402 (2): 229–239.
Published: 12 February 2007
... The Biochemical Society, London 2007 azurophilic granule exocytosis myeloperoxidase Rab27a small GTPase synaptotagmin-like protein (Slp) Human neutrophils play a central role in innate immunity by combating bacterial and fungal infections. Neutrophils kill micro-organisms by releasing...
Articles
Biochem J (2006) 399 (1): 111–119.
Published: 13 September 2006
... kinase C, which then promotes fusion pore dilation until full granule collapse occurs. Our results demonstrate that the transition between ‘kiss and run’ and ‘full collapse’ exocytosis serves a vital physiological regulation in neuroendocrine chromaffin cells and help effect a proper acute stress...
Articles
Biochem J (2005) 391 (3): 699–710.
Published: 25 October 2005
... (phosphoinositide 3-kinase) inhibitor. This supports the view that PI3K plays a differential role in the secretion of prostate secretory markers. In conclusion, we present evidence that JFC1 differentially regulates the secretion of PSAP and PSA, and that Rab27a and PI3K play a central role in the exocytosis...
Articles
Biochem J (2005) 387 (1): 195–202.
Published: 22 March 2005
...Yuk M. LEUNG; Youhou KANG; Fuzhen XIA; Laura SHEU; Xiaodong GAO; Huanli XIE; Robert G. TSUSHIMA; Herbert Y. GAISANO We have shown that SNARE (soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor) proteins not only participate directly in exocytosis, but also regulate...
Articles
Biochem J (2005) 385 (3): 721–727.
Published: 24 January 2005
... syncollin KO mice was reduced by 50%. This reduction was accounted for predominantly by a loss of later, ‘secondary’ fusion events between zymogen granules and other granules that had already fused with the plasma membrane. We conclude that syncollin is required for efficient exocytosis in the pancreatic...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2004) 381 (1): 13–18.
Published: 22 June 2004
... , 22 ] have demonstrated that the expression of the insulin exocytosis machinery, such as SNARE (soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor) proteins, is impaired in diabetic Goto–Kakizaki (GK) rat islets. Therefore, it is conceivable that there must be impairments...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2004) 380 (3): 897–905.
Published: 15 June 2004
... and appeared to exist as homooligomers. Hence, the lack of effect of syncollin–GFP on regulated exocytosis suggests that the GFP tag interferes in a subtler manner with the properties of the assembled protein. 1 To whom correspondence should be addressed (e-mail jme1000@cam.ac.uk ). 13 11 2003...
Articles
Biochem J (2004) 380 (3): 875–879.
Published: 15 June 2004
...Mitsunori FUKUDA Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Sytisoforms acts as the major Ca 2+ sensor for dense-core vesicle exocytosis. In the present...
Articles
Biochem J (2003) 375 (2): 433–440.
Published: 15 October 2003
... vesicle exocytosis. Several accessory factors have been described that associate with the synaptic SNAREs and modulate core complex assembly or mediate Ca 2+ regulation. One such factor, Snapin, has been reported to be a brain-specific protein that interacts with SNAP25, and regulates association...
Articles
Biochem J (2003) 373 (3): 641–659.
Published: 01 August 2003
...Alistair T. R. SIM; Monique L. BALDWIN; John A. P. ROSTAS; Jeff HOLST; Russell I. LUDOWYKE Modulation of exocytosis is integral to the regulation of cellular signalling, and a variety of disorders (such as epilepsy, hypertension, diabetes and asthma) are closely associated with pathological...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2003) 369 (2): 319–329.
Published: 15 January 2003
... stimulated by many cell surface receptors that catalyses the hydrolysis of phosphatidylcholine (PC) to generate the second messenger phosphatidate and choline (Cho) [1 3]. Phosphatidate has been implicated in many cellular functions, including exocytosis, endocytosis, remodelling of the actin cytoskeleton...
Articles
Biochem J (2002) 368 (2): 405–413.
Published: 01 December 2002
...Patricia ÑECO; Anabel GIL; María del MAR FRANCÉS; Salvador VINIEGRA; Luis M. GUTIÉRREZ Bovine adrenomedullary cells in culture have been used to study the role of myosin in vesicle transport during exocytosis. Amperometric determination of calcium-dependent catecholamine release from individual...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2002) 368 (1): 223–232.
Published: 15 November 2002
... The Biochemical Society, London ©2002 2002 exocytosis islets phosphorylation protein kinase C soluble NSF ( N -ethylmaleimide-sensitive fusion protein)-attachment protein receptor (SNARE) Abbreviations used: BIS, bisindolylmaleimide I; BoNT, botulinum neurotoxin; CaMKII, Ca 2+ /calmodulin...
Articles
Biochem J (2002) 366 (1): 1–13.
Published: 15 August 2002
...Ward C. TUCKER; Edwin R. CHAPMAN The Ca 2+ -binding synaptic-vesicle protein synaptotagmin I has attracted considerable interest as a potential Ca 2+ sensor that regulates exocytosis from neurons and neuroendocrine cells. Recent studies have shed new light on the structure, biochemical/biophysical...
Articles
Biochem J (2002) 365 (1): 173–180.
Published: 01 July 2002
...Mitsunori FUKUDA; Yukie OGATA; Chika SAEGUSA; Eiko KANNO; Katsuhiko MIKOSHIBA Synaptotagmin VII (Syt VII) has been proposed to regulate several different types of Ca 2+ -dependent exocytosis, but its subcellular localization (lysosome or plasma membrane) and the number of alternative splicing...
Articles
Biochem J (2002) 364 (2): 343–347.
Published: 01 June 2002
...Gareth J.O. EVANS; Alan MORGAN The secretory vesicle cysteine string proteins (CSPs) are members of the DnaJ family of chaperones, and function at late stages of Ca 2+ -regulated exocytosis by an unknown mechanism. To determine novel binding partners of CSPs, we employed a pull-down strategy from...
Articles
Biochem J (2002) 362 (2): 273–279.
Published: 22 February 2002
...Thierry COPPOLA; Véronique PERRET-MENOUD; Sonia GATTESCO; Sarah MAGNIN; Isabel POMBO; Ulrich BLANK; Romano REGAZZI Rab3 GTPases regulate exocytosis of neurons, endocrine and exocrine cells. In the present paper, we report a system to measure the guanine nucleotide status of Rab3 proteins in living...
Articles
Biochem J (2001) 356 (3): 843–850.
Published: 08 June 2001
... should be addressed (e-mail jme1000@cus.cam.ac.uk ). 12 12 2000 23 3 2001 10 4 2001 The Biochemical Society, London ©2001 2001 exocrine pancreas exocytosis secretion secretory granule Biochem. J. (2001) 356, 843 850 (Printed in Great Britain) 843 Cholesterol...
Articles
Biochem J (2001) 356 (1): 287–296.
Published: 08 May 2001
...Jef A. PINXTEREN; Bastien D. GOMPERTS; Danise ROGERS; Scott E. PHILLIPS; Peter E. R. TATHAM; Geraint M. H. THOMAS Mast cells permeabilized by streptolysin O undergo exocytosis when stimulated with Ca 2+ and guanosine 5′-[γ-thio]triphosphate but become progressively refractory to this stimulus...
Articles
Biochem J (2001) 354 (2): 323–330.
Published: 22 February 2001
... A " receptors. Key words: adenosine, exocytosis, glucose transport, insulin. associated with low surface accessibility and glucose transport, whereas activation of G i promotes the fusion of GLUT4 vesicles at the plasma membrane, increasing surface accessibility and glucose transport [4 6]. Short-term and long...
Articles
Biochem J (2000) 352 (2): 311–317.
Published: 24 November 2000
... degranulation. While the overall effect is still a decrease in the release of mast cell mediators, explaining the in ŠiŠo findings, the present study proposes a novel link between cPLA # and the degranulation machinery. Key words: anaphylaxis, exocytosis, leukotrienes, prosta- glandins. enter the circulation...
Articles
Biochem J (2000) 352 (2): 353–361.
Published: 24 November 2000
... induced by low [Ca ext [7]. These data suggest that, despite its inhibition by high [Ca i , PTH secretion occurs through regulated exocytosis. Abbreviations used: BAPTA/AM, bis-(o-aminophenoxy)ethane-N,N,N«,N«-tetra-acetic acid tetrakis(acetoxymethyl ester) ; [Ca2+]ext, extracellular calcium concentration...
Articles
Biochem J (2000) 351 (1): 33–37.
Published: 26 September 2000
... of regulated exocytosis. Extensive reverse transcription–PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2α nor DOC2β is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely...
Articles
Biochem J (2000) 350 (2): 337–352.
Published: 23 August 2000
...William E. HUGHES; Frank T. COOKE; Peter J. PARKER Advances in our understanding of the roles of phosphatidylinositol phosphates in controlling cellular functions such as endocytosis, exocytosis and the actin cytoskeleton have included new insights into the phosphatases that are responsible...
Articles
Biochem J (1999) 343 (3): 571–577.
Published: 25 October 1999
... to photolabel and the increase in glucose transport activity. These results suggest that R s G s and R i G i modulate insulin-stimulated glucose transport by influencing the extent to which GLUT4 is associated with occluded vesicles attached to the plasma membrane during exocytosis, perhaps by regulating...
Articles
Biochem J (1999) 343 (1): 151–157.
Published: 24 September 1999
... translocation of GLUT4 to the plasma membrane. 1 Deceased. 2 To whom correspondence should be addressed (e-mail ivar.walaas@basalmed.uio.no ). 22 1 1999 28 6 1999 15 7 1999 The Biochemical Society, London © 1999 1999 exocytosis fat cells glucose transport protein...
Articles
Biochem J (1999) 342 (3): 497–501.
Published: 05 September 1999
...Rory R. DUNCAN; Andrew C. DON-WAUCHOPE; Sompol TAPECHUM; Michael J. SHIPSTON; Robert H. CHOW; Peter ESTIBEIRO Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique...
Articles
Biochem J (1999) 342 (3): 707–714.
Published: 05 September 1999
...Lee P. HAYNES; Alan MORGAN; Robert D. BURGOYNE The target-SNARE syntaxin 1A is an essential component of the core machinery required for regulated exocytosis (where SNARE is the soluble N -ethylmaleimide-sensitive fusion protein-attachment protein receptor). Syntaxin 1A interacts with a variety...
Articles
Biochem J (1999) 342 (2): 353–360.
Published: 24 August 1999
...Michael L. FITZGERALD; Guy L. REED In platelets and other secretory cells, protein kinase C (PKC) plays a role in exocytosis stimulated by physiological extracellular signals, although its linkage to the secretory machinery is poorly understood. We investigated whether Rab6, a GTP-binding protein...
Articles
Biochem J (1999) 341 (1): 179–183.
Published: 24 June 1999
...Nathalie CHARVIN; Geoff WILLIAMS; Robert D. BURGOYNE Doc2 is a C2-domain-containing protein that is highly expressed in the nervous system and has a constitutively expressed isoform. It has been implicated as a potential Ca 2+ sensor in regulated exocytosis, and has been suggested to be associated...
Articles
Biochem J (1999) 339 (1): 159–165.
Published: 25 March 1999
... 1999 exocytosis islet neurotoxin regulated release SNARE Biochem. J. (1999) 339, 159 165 (Printed in Great Britain) 159 SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion Carmen GONELLE-GISPERT*, Philippe A. HALBAN*, Heiner NIEMANN...
Articles
Biochem J (1999) 338 (3): 583–589.
Published: 08 March 1999
... The Biochemical Society, London © 1999 1999 affinity chromatography calcium-binding protein cDNA cloning degranulation exocytosis Biochem. J. (1999) 338, 583 589 (Printed in Great Britain) 583 Three distinct anti-allergic drugs, amlexanox, cromolyn and tranilast, bind to S100A12 and S100A13...