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Keywords: enzyme kinetics
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Biochem J (2023) 480 (4): 259–281.
Published: 23 February 2023
... Press Limited on behalf of the Biochemical Society 2023 bacterial metabolism enzyme kinetics molecular docking protein crystallography site-directed mutagenesis structure–function relationships As a first step to assess PA4189 aldehyde specificity, its activity was determined at pH 8...
Includes: Supplementary data
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Biochem J (2021) 478 (17): 3185–3204.
Published: 07 September 2021
... (purple). Experiments were performed using p97 proteins at 4.17 nM hexamer and 30–750 µM ATP. All error bars indicate ± SD. anti-caner cryo-electron microscopy enzyme kinetics p97/VCP p97, also known as VCP (valosin-containing protein), TER (translational endoplasmic reticulum ATPase...
Includes: Supplementary data
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Biochem J (2020) 477 (1): 99–110.
Published: 08 January 2020
... Scientific) for glucose, cellobiose and cellotriose against a six-point combined external standard curve. cellobiohydrolase cellulose enzyme kinetics inhibition processivity proofreading Cellobiohydrolases (CBHs) are an industrially important group of glycoside hydrolase (GH) enzymes...
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Biochem J (2019) 476 (15): 2157–2172.
Published: 05 August 2019
.... Cel6A cellobiohydrolases cellulose enzyme kinetics GH6 hydrolysis Fungal degradation of cellulosic biomass plays a major role in the natural carbon cycle. The degradation process involves secretion of enzymes (cellulases) that cleave the β-1,4-glycosidic bonds in cellulose and hence...
Includes: Supplementary data
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Biochem J (2019) 476 (10): 1445–1463.
Published: 21 May 2019
...@actrec.gov.in ) * The author is deceased and is contributing posthumously. 20 3 2019 23 4 2019 26 4 2019 29 4 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 allostery apoptosis enzyme kinetics HtrA4 serine...
Includes: Supplementary data
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Biochem J (2019) 476 (4): 719–732.
Published: 28 February 2019
...-31G(d,p) basis, set with the Gaussian 09 software package [ 45 ]. density functional theory enzyme kinetics enzyme–substrate interactions molecular dynamics thermodynamics translation factors Chiral discrimination of amino acids is essential for the two connected steps of protein...
Includes: Supplementary data
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Biochem J (2018) 475 (20): 3221–3238.
Published: 23 October 2018
... © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 cancer metabolism enzyme inhibitor enzyme kinetics enzyme mutation IDH1 molecular dynamics GPU-enabled MD simulations were performed with NVIDIA GK110 (GeForce GTX Titan) GPUs using...
Includes: Supplementary data
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Biochem J (2018) 475 (16): 2611–2620.
Published: 29 August 2018
... study opens up the possibility of studying the influence of factors affecting transition states in real time and paves the way for the rational design of enzymes with enhanced efficiency. * These authors contributed equally to this work. bacterial adhesion dynamic force spectroscopy enzyme...
Includes: Multimedia, Supplementary data
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Biochem J (2018) 475 (6): 1141–1158.
Published: 26 March 2018
... (PerkinElmer), and then performing liquid scintillation counting with a 1215 Rackbeta instrument (LKB Wallac). © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 allosteric regulation cation transporter enzyme kinetics membrane-bound...
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Biochem J (2018) 475 (1): 137–150.
Published: 05 January 2018
... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 enzyme kinetics lysine biosynthesis oligomerisation protein evolution Figure 2. X-ray crystallographic structures determined of Nme -, Smo -, and Ath -DHDPR enzymes. All structures comprise...
Includes: Supplementary data
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Biochem J (2018) 475 (1): 225–245.
Published: 05 January 2018
... Merrill ( rmerrill@uoguelph.ca ) 23 10 2017 30 11 2017 4 12 2017 5 12 2017 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 bacterial toxins crystallography DNA modification enzyme kinetics Pathogenic bacteria...
Includes: Supplementary data
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Biochem J (2017) 474 (14): 2417–2433.
Published: 06 July 2017
...) with increasing final concentrations of LLVY-AMC substrate (0.015, 0.03, 0.06, 0.125, 0.25 and 0.5 mM). The steady-state (Michaelis–Menten) kinetic constants ( V max , K m , k cat / K m ) were calculated using the Enzyme Kinetics Module 1.1 (Sigma Plot, SPSS, Chicago). The effect of pH on KLK9...
Includes: Supplementary data
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Biochem J (2017) 474 (2): 247–258.
Published: 06 January 2017
... 11 2016 2 11 2016 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 enzyme kinetics metal transport nitrile hydratase protein biosynthesis Nitrile hydratases (NHases, EC 4.2.1.84) are metalloenzymes that catalyze...
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Biochem J (2016) 473 (16): 2471–2483.
Published: 11 August 2016
... ABC transporter ATPase inhibition enzyme kinetics haemolysin protein translocation RTX type I secretion system Type 1 secretion systems (T1SS) are membrane-embedded transport machineries involved in the secretion of a wide range of substrates in Gram-negative bacteria. Substrate...
Includes: Supplementary data
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Biochem J (2016) 473 (15): 2395–2411.
Published: 28 July 2016
... use. © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 coagulation Factor IX conformational changes crystal structure enzyme kinetics substrate-binding site thermal shift assay Blood coagulation serves to react on vessel...
Includes: Supplementary data
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Biochem J (2016) 473 (10): 1423–1431.
Published: 11 May 2016
...@ekmd.huji.ac.il ). 23 3 2015 17 3 2016 21 3 2016 21 3 2016 © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 ALDH1A2 enzyme kinetics retinoic acid signalling retinaldehyde dehydrogenase vitamin A metabolism Retinoic...
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Biochem J (2016) 473 (5): 593–603.
Published: 24 February 2016
... synthase isoforms are found in Arabidopsis thaliana each with specific substrate preferences and sensitivity to inhibitors and activators. Arabidopsis thaliana ceramide enzyme kinetics plant biochemistry sphingolipid Sphingolipids are a unique subset of membrane lipids...
Includes: Supplementary data
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Biochem J (2014) 462 (3): 525–537.
Published: 22 August 2014
... on the phosphorylation state. enzyme kinetics glycogen synthase Monod–Wyman–Changeux rate equation parameter optimization weight estimation To date a number of enzymes in pathways such as glycolysis (phosphofructokinase, EC 2.7.1.11 [ 1 ]), glycogen metabolism (glycogen phosphorylase, EC 2.4.1.1 [ 2...
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Biochem J (2014) 461 (3): 453–459.
Published: 10 July 2014
... ) enzymes respectively [ 28 ]. acetylpolyamine oxidase (APAO) enzyme catalysis enzyme kinetics polyamine spermine oxidase (SMO) substrate specificity The natural PAs (polyamines) Put (putrescine), Spd (spermidine) and Spm (spermine) are regulators of cell growth and proliferation...
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Biochem J (2014) 459 (1): 37–45.
Published: 14 March 2014
... the interplay between the three native substrates and show how harmful oxidase activity is avoided in aerobic environments. enzyme kinetics flavin molecular evolution stopped flow thymidylate synthase time-resolved spectroscopy We exploited this spectral change to study the binding kinetics...
Includes: Supplementary data
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Biochem J (2013) 453 (2): 281–290.
Published: 28 June 2013
... recognition and modification by a representative small RNA 2′- O -methyltransferase. enzyme kinetics methyltransferase miRNA 2′- O -ribose methylation RNA modification siRNA 12 11 2012 23 4 2013 29 4 2013 29 4 2013 © The Authors Journal compilation © 2013 Biochemical...
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Biochem J (2012) 446 (3): 489–497.
Published: 28 August 2012
... forms CYP1A2–CYP1A2 complexes that exhibit altered catalytic activity. © The Authors Journal compilation © 2012 Biochemical Society 2012 bioluminescence resonance energy transfer (BRET) cytochrome P450 enzyme kinetics homomeric P450–P450 complexes protein cross-linking protein–protein...
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Biochem J (2012) 442 (3): 621–629.
Published: 24 February 2012
... with overnight culture to a D 600 of 0.05 and incubated at 37°C. The progress of growth was monitored by measuring the D 600 . aromatic prenyltransferase enzyme kinetics 4-hydroxybenzoate solanesyltransferase solanesyl diphosphate (SDP) plastoquinone-9 Synechocystis sp. PCC6803 PQ-9...
Includes: Supplementary data
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Biochem J (2011) 440 (2): 203–215.
Published: 14 November 2011
... normal GK, whereas its TF was doubled by glucose saturation. However, the enzyme kinetics, GKRP inhibition, TF enhancement by glucose and responsiveness to GKA of the selected mutants varied greatly. Two predominant response patterns were identified accounting for nearly all mutants: (i) GK mutants...
Includes: Supplementary data
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Biochem J (2010) 430 (3): 539–549.
Published: 27 August 2010
...@imtech.res.in ). 4 1 2010 10 6 2010 24 6 2010 24 6 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 autophosphorylation bacterium enzyme kinetics NTP synthesis nucleoside diphosphate kinase phosphotransfer activity site-directed mutagenesis...
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Biochem J (2010) 425 (3): 585–593.
Published: 15 January 2010
... The Authors Journal compilation © 2010 Biochemical Society 2010 aromatic aldehyde aryl-alcohol oxidase (AAO) enzyme kinetics gem -diol GC-MS 1 H-NMR Lignin removal is a central process for recycling of the carbon fixed by photosynthesis in land ecosystems. Lignin protects plant...
Includes: Supplementary data
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Biochem J (2009) 422 (3): 455–462.
Published: 27 August 2009
... are important in the metabolism of endogenous as well as xenobiotic compounds, changes in the activity of these enzymes may have major implications for health. cytosolic sulfotransferase enzyme kinetics enzyme inhibition kynurenic acid tryptophan metabolism © The Authors Journal compilation ©...
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Biochem J (2007) 402 (3): 575–580.
Published: 26 February 2007
... spectropolarimeter as described previously [ 25 , 26 ]. The flavin content of each mutant enzyme was determined spectroscopically as outlined previously and is given in Table 1 [ 9 , 27 ]. enzyme kinetics haem biosynthesis protoporphyrin protoporphyrinogen IX oxidase stopped-flow fluorescence variegate...
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