... in this manner were used for other experiments, unless stated. enzyme catalysis fluorescence anisotropy decays molecular dynamics simulations mutational studies Helicobacter pylori ( H. pylori ) is a microaerophillic, Gram-negative bacterium that infects nearly half of the world's population...

...-hydroxybenzoyl-CoA TE, both of which fold in the same hotdog tertiary structure and catalyze the hydrolysis reaction of thioester bond, we have proposed a two-step catalytic mechanism for acyl-ACP TE that involves an enzyme-bound anhydride intermediate. acyl-ACP thioesterase enzyme catalysis enzyme...

... of Sh14. Moreover, the structural changes allow the strengthening of the two salt bridges between Glu 66 and Lys 104 at the dimer interface that triggers an allosteric effect and the communication between the hydrophobic sites. allosteric regulation enzyme catalysis enzyme kinetics enzyme structure...

... Journal compilation © 2014 Biochemical Society 2014 cofactor crystal structure enzyme catalysis lipoyl synthase radical S -adenosylmethionine (SAM) sulfur Thermosynechococcus elongatus 1 These authors are joint first authors for this paper. 2 To whom correspondence...

... et al. [ 23 , 24 ]. Recombinant SMO proteins were purified according to Tavladoraki et al. [ 13 ]. The purity of recombinant SMO proteins was assessed by SDS/PAGE electrophoretic analysis. acetylpolyamine oxidase (APAO) enzyme catalysis enzyme kinetics polyamine spermine oxidase (SMO...

... the catalytic cycle, which may exemplify a trait of E1o enzymes. bacterial metabolism decarboxylase dehydrogenase enzyme catalysis Mycobacterium protein dynamics X-ray crystallography Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved mechanisms...

...Nyssa Drinkwater; Christine L. Gee; Munish Puri; Kevin R. Criscione; Michael J. McLeish; Gary L. Grunewald; Jennifer L. Martin Substrate specificity is critically important for enzyme catalysis. In the adrenaline-synthesizing enzyme PNMT (phenylethanolamine N -methyltransferase), minor changes...

... network. In the GSTD (Delta class GST) studied, adGSTD4-4, the network consisted of His 38 , Met 39 , Asn 47 , Gln 49 , His 50 and Cys 51 . In addition to contributing to glutathione binding, this region also had major effects on enzyme catalysis, as shown by changes in kinetic parameters and substrate...

... that of the selenoenzymes. 1 To whom correspondence should be addressed (email luise.krauth-siegel@bzh.uni-heidelberg.de ). 19 2 2007 13 4 2007 25 4 2007 25 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 enzyme catalysis glutathione peroxidase-type...

... behaviours for the two forms), the calcu- lated reaction rate can exhibit a bell-shaped dependence on surfactant concentration. The effect of the partition of enzyme and substrate is described, as is that of enzyme efficiency in the various pseudo-phases. Key words: enzyme catalysis, kinetics models...