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Articles

LMAN1–MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin

Yuan Zhang, Min Zhu, Chunlei Zheng, Wei Wei, Brian T. Emmer, Bin Zhang

Biochem J (2022) 479 (7): 839–855.

DOI: https://doi.org/10.1042/BCJ20220055

Published: 11 April 2022

.... The AAT Z variant forms polymer within the endoplasmic reticulum (ER) of hepatocytes and results in reduction in AAT secretion and severe disease. Previous studies demonstrated a secretion defect of AAT in LMAN1 deficient cells, and mild decreases in AAT levels in male LMAN1 and MCFD2 deficient mice...

View article titled, LMAN1–MCFD2 complex is a cargo receptor for the ER-Golgi transport of α1-antitrypsin

Includes: Supplementary data

Articles

SLC26A9 is selected for endoplasmic reticulum associated degradation (ERAD) via Hsp70-dependent targeting of the soluble STAS domain

Patrick G. Needham, Jennifer L. Goeckeler-Fried, Casey Zhang, Zhihao Sun, Adam R. Wetzel, Carol A. Bertrand, Jeffrey L. Brodsky

Biochem J (2021) 478 (24): 4203–4220.

DOI: https://doi.org/10.1042/BCJ20210644

Published: 23 December 2021

... for other channels and transporters, we first noted that a substantial fraction of SLC26A9 is targeted for endoplasmic reticulum associated degradation (ERAD), which destroys folding-compromised proteins in the early secretory pathway. We next discovered that ERAD selection requires the Hsp70 chaperone...

View article titled, SLC26A9 is selected for endoplasmic reticulum associated degradation (ERAD) via Hsp70-dependent targeting of the soluble STAS domain

Includes: Supplementary data

Articles

Functional role of TRPC6 and STIM2 in cytosolic and endoplasmic reticulum Ca 2+ content in resting estrogen receptor-positive breast cancer cells

Jose Sanchez-Collado, Jose J. Lopez, Lucia Gonzalez-Gutierrez, Carlos Cantonero, Isaac Jardin, Ginés M. Salido, Juan A. Rosado

Biochem J (2020) 477 (17): 3183–3197.

DOI: https://doi.org/10.1042/BCJ20200560

Published: 04 September 2020

... of biotinylation, Ca 2+ imaging as well as protein knockdown and overexpression of a dominant-negative TRPC6 mutant (TRPC6dn) to show that TRPC6 and STIM2 are required for the maintenance of cytosolic and endoplasmic reticulum Ca 2+ content under resting conditions in ER+ breast cancer MCF7 cells. These cells...

View article titled, Functional role of TRPC6 and STIM2 in cytosolic and endoplasmic reticulum Ca 2+ content in resting estrogen receptor-positive breast cancer cells

Includes: Supplementary data

Articles

TMCC3 localizes at the three-way junctions for the proper tubular network of the endoplasmic reticulum

Sindhu Wisesa, Yasunori Yamamoto, Toshiaki Sakisaka

Biochem J (2019) 476 (21): 3241–3260.

DOI: https://doi.org/10.1042/BCJ20190359

Published: 11 November 2019

...Sindhu Wisesa; Yasunori Yamamoto; Toshiaki Sakisaka The tubular network of the endoplasmic reticulum (ER) is formed by connecting ER tubules through three-way junctions. Two classes of the conserved ER membrane proteins, atlastins and lunapark, have been shown to reside at the three-way junctions...

View article titled, TMCC3 localizes at the three-way junctions for the proper tubular network of the endoplasmic reticulum

Includes: Supplementary data

Articles

Mechanisms of lipid droplet biogenesis

In Collection

Editors' pick of review article highlights published in the Biochemical Journal in 2018 & 2019

Kent D. Chapman, Mina Aziz, John M. Dyer, Robert T. Mullen

Biochem J (2019) 476 (13): 1929–1942.

DOI: https://doi.org/10.1042/BCJ20180021

Published: 09 July 2019

... are believed to be derived by a budding and scission process from the surface of the endoplasmic reticulum, and this occurs concomitantly with the accumulation of neutral lipids, most often triacylglycerols and steryl esters. Overall, the mechanisms underlying LD biogenesis are difficult to generalize, in part...

View article titled, Mechanisms of lipid droplet biogenesis

Articles

Emerging roles of endoplasmic reticulum-resident selenoproteins in the regulation of cellular stress responses and the implications for metabolic disease

Alex B. Addinsall, Craig R. Wright, Sof Andrikopoulos, Chris van der Poel, Nicole Stupka

Biochem J (2018) 475 (6): 1037–1057.

DOI: https://doi.org/10.1042/BCJ20170920

Published: 20 March 2018

...Alex B. Addinsall; Craig R. Wright; Sof Andrikopoulos; Chris van der Poel; Nicole Stupka Chronic metabolic stress leads to cellular dysfunction, characterized by excessive reactive oxygen species, endoplasmic reticulum (ER) stress and inflammation, which has been implicated in the pathogenesis...

View article titled, Emerging roles of endoplasmic reticulum-resident selenoproteins in the regulation of cellular stress responses and the implications for metabolic disease

Articles

Molecular mechanisms of missense mutations that generate ectopic N-glycosylation sites in coagulation factor VIII

Wei Wei, Saurav Misra, Matthew V. Cannon, Renchi Yang, Xiaofan Zhu, Reid Gilmore, Min Zhu, Bin Zhang

Biochem J (2018) 475 (5): 873–886.

DOI: https://doi.org/10.1042/BCJ20170884

Published: 06 March 2018

... mutants, but impair the secretion of others. However, ectopic glycans that enhanced secretion could not functionally replace a native N -glycan in the same domain. Secretion-deficient mutants, but not mutants with elevated secretion levels, show increased association with the endoplasmic reticulum...

View article titled, Molecular mechanisms of missense mutations that generate ectopic N-glycosylation sites in coagulation factor VIII

Includes: Supplementary data

Articles

Methionine sulfoxide reductase B3 requires resolving cysteine residues for full activity and can act as a stereospecific methionine oxidase

Zhenbo Cao, Lorna Mitchell, Oliver Hsia, Miriam Scarpa, Stuart T. Caldwell, Arina D. Alfred, Alexandra Gennaris, Jean-François Collet, Richard C. Hartley, Neil J. Bulleid

Biochem J (2018) 475 (4): 827–838.

DOI: https://doi.org/10.1042/BCJ20170929

Published: 28 February 2018

... function. The enzyme methionine sulfoxide reductase (Msr) reverses this modification. Here, we characterise the mammalian enzyme Msr B3. There are two splice variants of this enzyme that differ only in their N-terminal signal sequence, which directs the protein to either the endoplasmic reticulum (ER...

View article titled, Methionine sulfoxide reductase B3 requires resolving cysteine residues for full activity and can act as a stereospecific methionine oxidase

Articles

IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design

Chloe L. Stoyle, Paul E. Stephens, David P. Humphreys, Sam Heywood, Katharine Cain, Neil J. Bulleid

Biochem J (2017) 474 (18): 3179–3188.

DOI: https://doi.org/10.1042/BCJ20170342

Published: 08 September 2017

... Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . antibodies antibody folding antibody production antibody secretion endoplasmic reticulum The ability to humanise rodent antibodies efficiently along...

View article titled, IgG light chain-independent secretion of heavy chain dimers: consequence for therapeutic antibody production and design

Articles

The membrane topology of vitamin K epoxide reductase is conserved between human isoforms and the bacterial enzyme

Zhenbo Cao, Marcel van Lith, Lorna J. Mitchell, Marie Anne Pringle, Kenji Inaba, Neil J. Bulleid

Biochem J (2016) 473 (7): 851–858.

DOI: https://doi.org/10.1042/BJ20151223

Published: 29 March 2016

..., with the large loop between transmembrane one and two facing the lumen of the endoplasmic reticulum (ER). We used a redox sensitive green fluorescent protein (GFP) fused to the N- or C-terminus to show that these regions face the cytosol, and introduction of glycosylation sites along with mixed disulfide...

View article titled, The membrane topology of vitamin K epoxide reductase is conserved between human isoforms and the bacterial enzyme

Articles

A ternary complex comprising FAK, PTPα and IP 3 receptor 1 functionally engages focal adhesions and the endoplasmic reticulum to mediate IL-1-induced Ca 2+ signalling in fibroblasts

Qin Wang, Yongqiang Wang, Gregory P. Downey, Sergey Plotnikov, Christopher A. McCulloch

Biochem J (2016) 473 (4): 397–410.

DOI: https://doi.org/10.1042/BJ20150907

Published: 09 February 2016

...Qin Wang; Yongqiang Wang; Gregory P. Downey; Sergey Plotnikov; Christopher A. McCulloch Ca 2+ release is tightly sequestered in eukaryotic cells to enable fine spatio-temporal control of signalling but how Ca 2+ release from the endoplasmic reticulum (ER) is linked to cell adhesions is not defined...

View article titled, A ternary complex comprising FAK, PTPα and IP 3 receptor 1 functionally engages focal adhesions and the endoplasmic reticulum to mediate IL-1-induced Ca 2+ signalling in fibroblasts

Includes: Supplementary data

Articles

The role of N-glycans and the C-terminal loop of the subunit rBAT in the biogenesis of the cystinuria-associated transporter

Mònica Rius, Laura Sala, Josep Chillarón

Biochem J (2016) 473 (3): 233–244.

DOI: https://doi.org/10.1042/BJ20150846

Published: 25 January 2016

... that impair biogenesis of the transporter cause type I cystinuria. It has been shown that upon assembly, b 0,+ AT prevents degradation and promotes folding of rBAT; then, rBAT traffics b 0,+ AT from the endoplasmic reticulum (ER) to the plasma membrane. The role of the N-glycans of rBAT and of its C-terminal...

View article titled, The role of N-glycans and the C-terminal loop of the subunit rBAT in the biogenesis of the cystinuria-associated transporter

Includes: Supplementary data

Articles

Cocaine inhibits store-operated Ca 2+ entry in brain microvascular endothelial cells: critical role for sigma-1 receptors

G. Cristina Brailoiu, Elena Deliu, Linda M. Console-Bram, Jonathan Soboloff, Mary E. Abood, Ellen M. Unterwald, Eugen Brailoiu

Biochem J (2016) 473 (1): 1–5.

DOI: https://doi.org/10.1042/BJ20150934

Published: 09 December 2015

...G. Cristina Brailoiu; Elena Deliu; Linda M. Console-Bram; Jonathan Soboloff; Mary E. Abood; Ellen M. Unterwald; Eugen Brailoiu Sigma-1 receptor (Sig-1R) is an intracellular chaperone protein with many ligands, located at the endoplasmic reticulum (ER). Binding of cocaine to Sig-1R has previously...

View article titled, Cocaine inhibits store-operated Ca 2+ entry in brain microvascular endothelial cells: critical role for sigma-1 receptors

Articles

Thiol-disulfide exchange between the PDI family of oxidoreductases negates the requirement for an oxidase or reductase for each enzyme

Ojore B.V. Oka, Hui Y. Yeoh, Neil J. Bulleid

Biochem J (2015) 469 (2): 279–288.

DOI: https://doi.org/10.1042/BJ20141423

Published: 06 July 2015

... is that only certain members need to be oxidized or reduced to maintain function. disulfide formation electron transfer endoplasmic reticulum protein disulfide isomerase redox potential thiol-disulfide exchange A family of proteins, collectively termed the protein disulfide isomerases (PDIs...

View article titled, Thiol-disulfide exchange between the PDI family of oxidoreductases negates the requirement for an oxidase or reductase for each enzyme

Articles

Differential calcium handling by the cis and trans regions of the Golgi apparatus

Francisco J. Aulestia, María Teresa Alonso, Javier García-Sancho

Biochem J (2015) 466 (3): 455–465.

DOI: https://doi.org/10.1042/BJ20141358

Published: 06 March 2015

...+ probes to specifically measure Ca 2+ in the cis/cis- to -medial -Go (cGo) or the trans -Go (tGo). Ca 2+ homoeostasis in these compartments and in the endoplasmic reticulum (ER) has been studied and compared. Moreover, the relative size of each subcompartment was estimated from aequorin consumption. We...

View article titled, Differential calcium handling by the cis and trans regions of the Golgi apparatus

Includes: Multimedia, Supplementary data

Articles

A context-independent N -glycan signal targets the misfolded extracellular domain of Arabidopsis STRUBBELIG to endoplasmic-reticulum-associated degradation

Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Martina Dicker, Daniel Maresch, Friedrich Altmann, Richard Strasser

Biochem J (2014) 464 (3): 401–411.

DOI: https://doi.org/10.1042/BJ20141057

Published: 05 December 2014

...Silvia Hüttner; Christiane Veit; Ulrike Vavra; Jennifer Schoberer; Martina Dicker; Daniel Maresch; Friedrich Altmann; Richard Strasser N -glycosylation of proteins plays an important role in the determination of the fate of newly synthesized glycoproteins in the endoplasmic reticulum (ER). Specific...

View article titled, A context-independent N -glycan signal targets the misfolded extracellular domain of Arabidopsis STRUBBELIG to endoplasmic-reticulum-associated degradation

Articles

Cytochrome P450 system proteins reside in different regions of the endoplasmic reticulum

Ji Won Park, James R. Reed, Lauren M. Brignac-Huber, Wayne L. Backes

Biochem J (2014) 464 (2): 241–249.

DOI: https://doi.org/10.1042/BJ20140787

Published: 14 November 2014

...Ji Won Park; James R. Reed; Lauren M. Brignac-Huber; Wayne L. Backes Cytochrome P450 (P450) function is dependent on the ability of these enzymes to successfully interact with their redox partners, NADPH-cytochrome P450 reductase (CPR) and cytochrome b 5 , in the endoplasmic reticulum (ER). Because...

View article titled, Cytochrome P450 system proteins reside in different regions of the endoplasmic reticulum

Includes: Supplementary data

Articles

The RED light is on! New tools for monitoring Ca 2+ dynamics in the endoplasmic reticulum and mitochondria

Saverio Marchi, Paolo Pinton

Biochem J (2014) 464 (1): e5–e6.

DOI: https://doi.org/10.1042/BJ20141183

Published: 23 October 2014

...Saverio Marchi; Paolo Pinton In this issue of the Biochemical Journal , Wu et al. describe the characterization of new low-affinity Ca 2+ indicators for monitoring Ca 2+ levels in both the ER (endoplasmic reticulum) and mitochondria. In contrast with other ER Ca 2+ sensors, these indicators emit...

View article titled, The RED light is on! New tools for monitoring Ca 2+ dynamics in the endoplasmic reticulum and mitochondria

Articles

STIM1 regulates TRPC6 heteromultimerization and subcellular location

Letizia Albarrán, Natalia Dionisio, Esther Lopez, Ginés M. Salido, Pedro C. Redondo, Juan A. Rosado

Biochem J (2014) 463 (3): 373–381.

DOI: https://doi.org/10.1042/BJ20140523

Published: 10 October 2014

... by STIM1 is poorly understood. STIM1 is a single transmembrane protein that communicates the filling state of the endoplasmic reticulum to store-operated channels. STIM1 has been reported to regulate the activity of all of the TRPC family members, except TRPC7. TRPC6 has been predominantly associated...

View article titled, STIM1 regulates TRPC6 heteromultimerization and subcellular location

Articles

Cellular mechanisms underlying the increased disease severity seen for patients with long QT syndrome caused by compound mutations in KCNQ1

Stephen C. Harmer, Jagdeep S. Mohal, Alice A. Royal, William J. McKenna, Pier D. Lambiase, Andrew Tinker

Biochem J (2014) 462 (1): 133–142.

DOI: https://doi.org/10.1042/BJ20140425

Published: 24 July 2014

... with a severe clinical phenotype. We identify that the retention of compound mutant channel complexes in the endoplasmic reticulum may underlie why patients with this phenotype have an increased arrhythmic risk. We have investigated five compound heterozygous mutations and one homozygous mutation...

View article titled, Cellular mechanisms underlying the increased disease severity seen for patients with long QT syndrome caused by compound mutations in KCNQ1

Articles

Inactivation of mammalian Ero1α is catalysed by specific protein disulfide-isomerases

Colin Shepherd, Ojore B. V. Oka, Neil J. Bulleid

Biochem J (2014) 461 (1): 107–113.

DOI: https://doi.org/10.1042/BJ20140234

Published: 13 June 2014

...Colin Shepherd; Ojore B. V. Oka; Neil J. Bulleid Disulfide formation within the endoplasmic reticulum is a complex process requiring a disulfide exchange protein such as PDI (protein disulfide-isomerase) and a mechanism to form disulfides de novo . In mammalian cells, the major pathway for de novo...

View article titled, Inactivation of mammalian Ero1α is catalysed by specific protein disulfide-isomerases

Articles

Mature VLDL triggers the biogenesis of a distinct vesicle from the trans -Golgi network for its export to the plasma membrane

Tanvir Hossain, Aladdin Riad, Shaila Siddiqi, Sampath Parthasarathy, Shadab A. Siddiqi

Biochem J (2014) 459 (1): 47–58.

DOI: https://doi.org/10.1042/BJ20131215

Published: 14 March 2014

... endoplasmic reticulum post-Golgi VLDL transport vesicle (PG-VTV) trans- Golgi network (TGN) triacylglycerol very low-density lipoprotein (VLDL) Among its many functions, the liver is responsible for the conversion of potentially cytotoxic FFAs [non-esterified (‘free’) fatty acids] into more...

View article titled, Mature VLDL triggers the biogenesis of a distinct vesicle from the trans -Golgi network for its export to the plasma membrane

Articles

PCSK9 acts as a chaperone for the LDL receptor in the endoplasmic reticulum

Thea Bismo Strøm, Kristian Tveten, Trond P. Leren

Biochem J (2014) 457 (1): 99–105.

DOI: https://doi.org/10.1042/BJ20130930

Published: 10 December 2013

...Thea Bismo Strøm; Kristian Tveten; Trond P. Leren PCSK9 (proprotein convertase subtilisin/kexin type 9) binds to the LDLR (low-density lipoprotein receptor) at the cell surface and disrupts recycling of the LDLR. However, PCSK9 also interacts with the LDLR in the ER (endoplasmic reticulum...

View article titled, PCSK9 acts as a chaperone for the LDL receptor in the endoplasmic reticulum

Includes: Supplementary data

Articles

Reorientation of the first signal-anchor sequence during potassium channel biogenesis at the Sec61 complex

Helen R. Watson, Lydia Wunderley, Tereza Andreou, Jim Warwicker, Stephen High

Biochem J (2013) 456 (2): 297–309.

DOI: https://doi.org/10.1042/BJ20130100

Published: 08 November 2013

...Helen R. Watson; Lydia Wunderley; Tereza Andreou; Jim Warwicker; Stephen High The majority of the polytopic proteins that are synthesized at the ER (endoplasmic reticulum) are integrated co-translationally via the Sec61 translocon, which provides lateral access for their hydrophobic TMs...

View article titled, Reorientation of the first signal-anchor sequence during potassium channel biogenesis at the Sec61 complex

Includes: Supplementary data

Articles

Peroxisome membrane proteins: multiple trafficking routes and multiple functions?

Frederica L. Theodoulou, Kristin Bernhardt, Nicole Linka, Alison Baker

Biochem J (2013) 451 (3): 345–352.

DOI: https://doi.org/10.1042/BJ20130078

Published: 12 April 2013

... and rates of PMP turnover [ 44 ]. 1 To whom correspondence should be addressed (email freddie.theodoulou@rothamsted.ac.uk ). 14 1 2013 18 2 2013 19 2 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 biogenesis endoplasmic reticulum peroxin...

View article titled, Peroxisome membrane proteins: multiple trafficking routes and multiple functions?

Articles

High-resolution NMR studies of structure and dynamics of human ERp27 indicate extensive interdomain flexibility

Nader T. Amin, A. Katrine Wallis, Stephen A. Wells, Michelle L. Rowe, Richard A. Williamson, Mark J. Howard, Robert B. Freedman

Biochem J (2013) 450 (2): 321–332.

DOI: https://doi.org/10.1042/BJ20121635

Published: 15 February 2013

...Nader T. Amin; A. Katrine Wallis; Stephen A. Wells; Michelle L. Rowe; Richard A. Williamson; Mark J. Howard; Robert B. Freedman ERp27 (endoplasmic reticulum protein 27.7 kDa) is a homologue of PDI (protein disulfide-isomerase) localized to the endoplasmic reticulum. ERp27 is predicted to consist...

View article titled, High-resolution NMR studies of structure and dynamics of human ERp27 indicate extensive interdomain flexibility

Includes: Supplementary data

Articles

Heat-shock proteins attenuate SERCA inactivation by the anti-apoptotic protein Bcl-2: possible implications for the ER Ca 2+ -mediated apoptosis

Elena S. Dremina, Victor S. Sharov, Christian Schöneich

Biochem J (2012) 444 (1): 127–139.

DOI: https://doi.org/10.1042/BJ20111114

Published: 26 April 2012

...Elena S. Dremina; Victor S. Sharov; Christian Schöneich We have demonstrated previously that Bcl-2 and Bcl-2Δ21, a C-terminally truncated Bcl-2 sequence, inactivate SERCA (sarcoplasmic/endoplasmic reticulum Ca 2+ -ATPase) 1 in isolated SR (sarcoplasmic reticulum), accompanied by a translocation...

View article titled, Heat-shock proteins attenuate SERCA inactivation by the anti-apoptotic protein Bcl-2: possible implications for the ER Ca 2+ -mediated apoptosis

Articles

Inhibition of protein translocation at the endoplasmic reticulum promotes activation of the unfolded protein response

Craig McKibbin, Alina Mares, Michela Piacenti, Helen Williams, Peristera Roboti, Marjo Puumalainen, Anna C. Callan, Karolina Lesiak-Mieczkowska, Stig Linder, Hanna Harant, Stephen High, Sabine L. Flitsch, Roger C. Whitehead, Eileithyia Swanton

Biochem J (2012) 442 (3): 639–648.

DOI: https://doi.org/10.1042/BJ20111220

Published: 24 February 2012

... tools for the dissection of complex biological processes. ES I (eeyarestatin I) is a novel modulator of ER (endoplasmic reticulum) function. In the present study, we show that in addition to acutely inhibiting ERAD (ER-associated degradation), ES I causes production of mislocalized polypeptides...

View article titled, Inhibition of protein translocation at the endoplasmic reticulum promotes activation of the unfolded protein response

Includes: Supplementary data

Articles

Stepwise proteolytic activation of type I procollagen to collagen within the secretory pathway of tendon fibroblasts in situ

Elizabeth G. Canty-Laird, Yinhui Lu, Karl E. Kadler

Biochem J (2012) 441 (2): 707–717.

DOI: https://doi.org/10.1042/BJ20111379

Published: 21 December 2011

... network). In the present study, brefeldin A treatment of tendon explant cultures showed that N-proteinase activity is present in the resulting fused ER (endoplasmic reticulum)–Golgi compartment, but that C-proteinase activity is restricted to the TGN in embryonic chick tendon fibroblasts. In late...

View article titled, Stepwise proteolytic activation of type I procollagen to collagen within the secretory pathway of tendon fibroblasts in situ

Articles

Determinants of the enzymatic activity and the subcellular localization of aspartate N-acetyltransferase

Gaëlle Tahay, Elsa Wiame, Donatienne Tyteca, Pierre J. Courtoy, Emile Van Schaftingen

Biochem J (2012) 441 (1): 105–112.

DOI: https://doi.org/10.1042/BJ20111179

Published: 14 December 2011

...Gaëlle Tahay; Elsa Wiame; Donatienne Tyteca; Pierre J. Courtoy; Emile Van Schaftingen Aspartate N-acetyltransferase (NAT8L, N-acetyltransferase 8-like), the enzyme that synthesizes N -acetylaspartate, is membrane-bound and is at least partially associated with the ER (endoplasmic reticulum...

View article titled, Determinants of the enzymatic activity and the subcellular localization of aspartate N-acetyltransferase

Includes: Supplementary data

Articles

Calcium homoeostasis modulator 1 (CALHM1) reduces the calcium content of the endoplasmic reticulum (ER) and triggers ER stress

Sonia Gallego-Sandín, María Teresa Alonso, Javier García-Sancho

Biochem J (2011) 437 (3): 469–475.

DOI: https://doi.org/10.1042/BJ20110479

Published: 13 July 2011

...Sonia Gallego-Sandín; María Teresa Alonso; Javier García-Sancho CALHM1 (calcium homoeostasis modulator 1), a membrane protein with similarity to NMDA ( N -methyl- D -aspartate) receptor channels that localizes in the plasma membrane and the ER (endoplasmic reticulum) of neurons, has been shown...

View article titled, Calcium homoeostasis modulator 1 (CALHM1) reduces the calcium content of the endoplasmic reticulum (ER) and triggers ER stress

Includes: Supplementary data

Articles

Cytosolic lysine residues enhance anterograde transport and activation of the erythropoietin receptor

Liron Yosha, Orly Ravid, Nathalie Ben-Califa, Drorit Neumann

Biochem J (2011) 435 (2): 509–518.

DOI: https://doi.org/10.1042/BJ20101876

Published: 29 March 2011

... demonstrated delayed glycan maturation kinetics upon substitution of the lysine residues. Moreover, VSVG-wt EPO-R, but not VSVG-5KR EPO-R, displayed endoplasmic reticulum-associated ubiquitination. Despite similar cell-surface EPO-binding levels of both receptors and the lack of EPO-induced ubiquitination...

View article titled, Cytosolic lysine residues enhance anterograde transport and activation of the erythropoietin receptor

Articles

Two distinct calcium pools in the endoplasmic reticulum of HEK-293T cells

Francisco J. Aulestia, Pedro C. Redondo, Arancha Rodríguez-García, Juan A. Rosado, Ginés M. Salido, Maria Teresa Alonso, Javier García-Sancho

Biochem J (2011) 435 (1): 227–235.

DOI: https://doi.org/10.1042/BJ20101427

Published: 15 March 2011

... Ca 2+ stores using targeted aequorins for selective measurements in different subcellular compartments. Both, HEK-293T [HEK (human embryonic kidney)-293 cells expressing the large T-antigen of SV40 (simian virus 40)] and HeLa cells accumulated Ca 2+ into the ER (endoplasmic reticulum) to near...

View article titled, Two distinct calcium pools in the endoplasmic reticulum of HEK-293T cells

Includes: Supplementary data

Articles

Beyond the endoplasmic reticulum: atypical GRP78 in cell viability, signalling and therapeutic targeting

Min Ni, Yi Zhang, Amy S. Lee

Biochem J (2011) 434 (2): 181–188.

DOI: https://doi.org/10.1042/BJ20101569

Published: 11 February 2011

...Min Ni; Yi Zhang; Amy S. Lee GRP78 (glucose-regulated protein of 78 kDa) is traditionally regarded as a major ER (endoplasmic reticulum) chaperone facilitating protein folding and assembly, protein quality control, Ca 2+ binding and regulating ER stress signalling. It is a potent anti-apoptotic...

View article titled, Beyond the endoplasmic reticulum: atypical GRP78 in cell viability, signalling and therapeutic targeting

Includes: Multimedia, Supplementary data

Articles

Identification of topological determinants in the N-terminal domain of transcription factor Nrf1 that control its orientation in the endoplasmic reticulum membrane

Yiguo Zhang, John D. Hayes

Biochem J (2010) 430 (3): 497–510.

DOI: https://doi.org/10.1042/BJ20100471

Published: 27 August 2010

...Yiguo Zhang; John D. Hayes Nrf1 [NF-E2 (nuclear factor-erythroid 2)-related factor 1] is a CNC (cap'n'collar) bZIP (basic-region leucine zipper) transcription factor that is tethered to ER (endoplasmic reticulum) and nuclear envelope membranes through its N-terminal signal peptide (residues 1–30...

View article titled, Identification of topological determinants in the N-terminal domain of transcription factor Nrf1 that control its orientation in the endoplasmic reticulum membrane

Includes: Supplementary data

Articles

Disulfide formation in plant storage vacuoles permits assembly of a multimeric lectin

Richard S. Marshall, Lorenzo Frigerio, Lynne M. Roberts

Biochem J (2010) 427 (3): 513–521.

DOI: https://doi.org/10.1042/BJ20091878

Published: 14 April 2010

...Richard S. Marshall; Lorenzo Frigerio; Lynne M. Roberts The ER (endoplasmic reticulum) has long been considered the plant cell compartment within which protein disulfide bond formation occurs. Members of the ER-located PDI (protein disulfide isomerase) family are responsible for oxidizing, reducing...

View article titled, Disulfide formation in plant storage vacuoles permits assembly of a multimeric lectin

Articles

Discovery of functional motifs in h-regions of trypanosome signal sequences

Josh Duffy, Bhargavi Patham, Kojo Mensa-Wilmot

Biochem J (2010) 426 (2): 135–145.

DOI: https://doi.org/10.1042/BJ20091277

Published: 09 February 2010

...Josh Duffy; Bhargavi Patham; Kojo Mensa-Wilmot N-terminal signal peptides direct secretory proteins into the ER (endoplasmic reticulum) of eukaryotes or the periplasmic space of prokaryotes. A hydrophobic core (h-region) is important for signal sequence function; however, the mechanism of h-region...

View article titled, Discovery of functional motifs in h-regions of trypanosome signal sequences

Includes: Supplementary data

Articles

A di-arginine motif contributes to the ER localization of the type I transmembrane ER oxidoreductase TMX4

Doris Roth, Emily Lynes, Jan Riemer, Henning G. Hansen, Nils Althaus, Thomas Simmen, Lars Ellgaard

Biochem J (2010) 425 (1): 195–208.

DOI: https://doi.org/10.1042/BJ20091064

Published: 14 December 2009

...Doris Roth; Emily Lynes; Jan Riemer; Henning G. Hansen; Nils Althaus; Thomas Simmen; Lars Ellgaard The thiol-disulfide oxidoreductases of the PDI (protein disulfide isomerase) family assist in disulfide-bond formation in the ER (endoplasmic reticulum). In the present study, we have shown...

View article titled, A di-arginine motif contributes to the ER localization of the type I transmembrane ER oxidoreductase TMX4

Includes: Supplementary data

Articles

Targeting of inositol 1,4,5-trisphosphate receptor to the endoplasmic reticulum by its first transmembrane domain

Evangelia Pantazaka, Colin W. Taylor

Biochem J (2010) 425 (1): 61–74.

DOI: https://doi.org/10.1042/BJ20091051

Published: 14 December 2009

...Evangelia Pantazaka; Colin W. Taylor Targeting of IP 3 R (inositol 1,4,5-trisphosphate receptors) to membranes of the ER (endoplasmic reticulum) and their retention within ER or trafficking to other membranes underlies their ability to generate spatially organized Ca 2+ signals. N-terminal...

View article titled, Targeting of inositol 1,4,5-trisphosphate receptor to the endoplasmic reticulum by its first transmembrane domain

Includes: Supplementary data

Articles

The plant endoplasmic reticulum: a cell-wide web

Imogen A. Sparkes, Lorenzo Frigerio, Nicholas Tolley, Chris Hawes

Biochem J (2009) 423 (2): 145–155.

DOI: https://doi.org/10.1042/BJ20091113

Published: 25 September 2009

...Imogen A. Sparkes; Lorenzo Frigerio; Nicholas Tolley; Chris Hawes The ER (endoplasmic reticulum) in higher plants forms a pleomorphic web of membrane tubules and small cisternae that pervade the cytoplasm, but in particular form a polygonal network at the cortex of the cell which may be anchored...

View article titled, The plant endoplasmic reticulum: a cell-wide web

Includes: Multimedia, Supplementary data

Articles

A small component of the endoplasmic reticulum is required for store-operated Ca 2+ channel activation in liver cells: evidence from studies using TRPV1 and taurodeoxycholic acid

Joel Castro, Edoardo C. Aromataris, Grigori Y. Rychkov, Greg J. Barritt

Biochem J (2009) 418 (3): 553–566.

DOI: https://doi.org/10.1042/BJ20081052

Published: 25 February 2009

...Joel Castro; Edoardo C. Aromataris; Grigori Y. Rychkov; Greg J. Barritt The question of whether the activation of SOCs (store-operated Ca 2+ channels) requires the whole or part of the ER (endoplasmic reticulum) has not been fully resolved. The role of a putative sub-compartment of the ER in SOC...

View article titled, A small component of the endoplasmic reticulum is required for store-operated Ca 2+ channel activation in liver cells: evidence from studies using TRPV1 and taurodeoxycholic acid

Includes: Supplementary data

Articles

Acyl-CoA-binding protein (ACBP) localizes to the endoplasmic reticulum and Golgi in a ligand-dependent manner in mammalian cells

Jesper S. Hansen, Nils J. Færgeman, Birthe B. Kragelund, Jens Knudsen

Biochem J (2008) 410 (3): 463–472.

DOI: https://doi.org/10.1042/BJ20070559

Published: 27 February 2008

... to characterize the localization and dynamics of ACBP in living cells. Results showed that ACBP targeted to the ER (endoplasmic reticulum) and Golgi in a ligand-binding-dependent manner. A variant Y28F/K32A-FACI-50, which is unable to bind acyl-CoA, did no longer show association with the ER and became segregated...

View article titled, Acyl-CoA-binding protein (ACBP) localizes to the endoplasmic reticulum and Golgi in a ligand-dependent manner in mammalian cells

Includes: Supplementary data

Articles

A transplanted NPVY sequence in the cytosolic domain of the erythropoietin receptor enhances maturation

Tal Nahari, Eran Barzilay, Koret Hirschberg, Drorit Neumann

Biochem J (2008) 410 (2): 409–416.

DOI: https://doi.org/10.1042/BJ20071297

Published: 12 February 2008

.... In the present study, we addressed the role of the EPO-R intracellular domain in exit from the ER (endoplasmic reticulum) and surface expression. A fusion protein between the thermo-reversible folding mutant of VSVG (vesicular-stomatitis-virus glycoprotein) (VSVGtsO45) and the EPO-R cytosolic domain [VSVG-WT...

View article titled, A transplanted NPVY sequence in the cytosolic domain of the erythropoietin receptor enhances maturation

Includes: Multimedia, Supplementary data

Articles

Sec22b-dependent assembly of endoplasmic reticulum Q-SNARE proteins

Takehiro Aoki, Masaki Kojima, Katsuko Tani, Mitsuo Tagaya

Biochem J (2008) 410 (1): 93–100.

DOI: https://doi.org/10.1042/BJ20071304

Published: 29 January 2008

... be classified into Q-SNARE and R-SNARE based on the structural property of their motifs. Assembly of four SNARE motifs (Qa, b, c and R) is supposed to trigger membrane fusion. We have previously shown that ER (endoplasmic reticulum)-localized syntaxin 18 (Qa) forms a complex with BNIP1 (Qb), p31/Use1 (Qc...

View article titled, Sec22b-dependent assembly of endoplasmic reticulum Q-SNARE proteins

Articles

The NHB1 (N-terminal homology box 1) sequence in transcription factor Nrf1 is required to anchor it to the endoplasmic reticulum and also to enable its asparagine-glycosylation

Yiguo Zhang, John M. Lucocq, Masayuki Yamamoto, John D. Hayes

Biochem J (2007) 408 (2): 161–172.

DOI: https://doi.org/10.1042/BJ20070761

Published: 14 November 2007

... homology box 1; residues 11–30), which tethers Nrf1 to the ER (endoplasmic reticulum). Electrophoresis resolved Nrf1 into two major bands of approx. 95 and 120 kDa. The 120-kDa Nrf1 form represents a glycosylated protein that was present exclusively in the ER and was converted into a substantially smaller...

View article titled, The NHB1 (N-terminal homology box 1) sequence in transcription factor Nrf1 is required to anchor it to the endoplasmic reticulum and also to enable its asparagine-glycosylation

Includes: Supplementary data

Articles

The reductase NCB5OR is responsive to the redox status in β-cells and is not involved in the ER stress response

Kevin Larade, Zhi-gang Jiang, Andre Dejam, Hao Zhu, H. Franklin Bunn

Biochem J (2007) 404 (3): 467–476.

DOI: https://doi.org/10.1042/BJ20061859

Published: 29 May 2007

...Kevin Larade; Zhi-gang Jiang; Andre Dejam; Hao Zhu; H. Franklin Bunn The novel reductase NCB5OR (NADPH cytochrome b 5 oxidoreductase) resides in the ER (endoplasmic reticulum) and may protect cells against ER stress. Levels of BiP (immunoglobulin heavy-chain-binding protein), CHOP (CCAAT/enhancer...

View article titled, The reductase NCB5OR is responsive to the redox status in β-cells and is not involved in the ER stress response

Articles

The C-terminus of cytochrome b 5 confers endoplasmic reticulum specificity by preventing spontaneous insertion into membranes

Matthew P. A. Henderson, Yeen Ting Hwang, John M. Dyer, Robert T. Mullen, David W. Andrews

Biochem J (2007) 401 (3): 701–709.

DOI: https://doi.org/10.1042/BJ20060990

Published: 12 January 2007

... oil tree [ Vernicia ( Aleurites ) fordii ] tail-anchored Cb5 (cytochrome b 5 ) target specifically to ER (endoplasmic reticulum) membranes both in vivo and in vitro [Hwang, Pelitire, Henderson, Andrews, Dyer and Mullen (2004) Plant Cell 16 , 3002–3019]. In the present study, we examine the targeting...

View article titled, The C-terminus of cytochrome b 5 confers endoplasmic reticulum specificity by preventing spontaneous insertion into membranes

Includes: Supplementary data

Articles

Biosynthesis of the dystonia-associated AAA + ATPase torsinA at the endoplasmic reticulum

Anna C. Callan, Sandra Bunning, Owen T. Jones, Stephen High, Eileithyia Swanton

Biochem J (2007) 401 (2): 607–612.

DOI: https://doi.org/10.1042/BJ20061313

Published: 21 December 2006

..., a single membrane spanning domain, and its C-terminus located in the ER (endoplasmic reticulum) lumen. However, in the present study we show that torsinA is not in fact an integral membrane protein. Instead we find that the mature protein associates peripherally with the ER membrane, most likely through...

View article titled, Biosynthesis of the dystonia-associated AAA + ATPase torsinA at the endoplasmic reticulum

Articles

Negative regulation of the Nrf1 transcription factor by its N-terminal domain is independent of Keap1: Nrf1, but not Nrf2, is targeted to the endoplasmic reticulum

Yiguo Zhang, Dorothy H. Crouch, Masayuki Yamamoto, John D. Hayes

Biochem J (2006) 399 (3): 373–385.

DOI: https://doi.org/10.1042/BJ20060725

Published: 13 October 2006

... controlled by the NTD (N-terminal domain) through its ability to direct Nrf1 to the endoplasmic reticulum. Ectopic expression of wild-type Nrf1 and mutants lacking either the NTD or portions of its Neh2-like subdomain into wild-type and mutant mouse embryonic fibroblasts indicated that Keap1 controls neither...

View article titled, Negative regulation of the Nrf1 transcription factor by its N-terminal domain is independent of Keap1: Nrf1, but not Nrf2, is targeted to the endoplasmic reticulum

Includes: Supplementary data

Articles

Wild-type and mutant ferroportins do not form oligomers in transfected cells

Ana Sofia Gonçalves, Françoise Muzeau, Rand Blaybel, Gilles Hetet, Fathi Driss, Constance Delaby, François Canonne-Hergaux, Carole Beaumont

Biochem J (2006) 396 (2): 265–275.

DOI: https://doi.org/10.1042/BJ20051682

Published: 15 May 2006

... associated with the endoplasmic reticulum and with Rab5-positive vesicles. However, this mutant is complex-glycosylated like the wt protein. D157G and G323V mutants have a defective iron export capacity as judged by their inability to deplete the intracellular ferritin content, whereas Q182H and delV162 have...

View article titled, Wild-type and mutant ferroportins do not form oligomers in transfected cells

Includes: Supplementary data

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