...Edwin T. Gibbs, II; Chad A. Lerner; Mark A. Watson; Hoi-Shan Wong; Akos A. Gerencser; Martin D. Brand Superoxide/hydrogen peroxide production by site I Q in complex I of the electron transport chain is conventionally assayed during reverse electron transport (RET) from ubiquinol to NAD. However...

... and heart mitochondria were isolated as described below. amino acid metabolism bioenergetics Buffer C electron transport chain mitochondria Differential centrifugation was used to isolate mitochondria from heart, liver, and skeletal muscle. Buffers A and B were utilized in all...

... in respiratory complex I were identified, and their effects on its flavin-site reactions were determined. Reduction of cluster N1a by NADH does not affect reactive oxygen species production by the flavin. electron transport chain iron–sulfur cluster mitochondrion NADH:quinone oxidoreductase (complex I...

... senescence model. Cellular aging appears to promote a Nox4 interaction with mitochondria that disrupts complex I in the electron transport chain and increases the detection of mitochondrial H 2 O 2 . Nox4 appears to maintain a highly interconnected mitochondrial network, which may influence mitochondrial...

..., stabilized mitochondrial membrane potential and decreased production of H 2 O 2 in mitochondria. High-resolution respirometry in permeabilized cells combined with native PAGE demonstrated that Nox4 specifically inhibits the activity of mitochondrial electron transport chain complex I, and this was associated...

... 2 5 2012 2 5 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 O -acetylserine(thiol)lyase cysteine cytochrome c oxidase (COX) electron transport chain mitochondrion sulfide toxicity Biosynthesis of cysteine in higher plants is catalysed by OAS...

... 344 rats. OXPHOS (oxidative phosphorylation) of intact mitochondria and cytochrome c oxidase activity in permeabilized mitochondria were determined with polarographic assays. The activities of the ETC (electron transport chain) complexes and the cytochrome content in solubilized mitochondria were...

... catalytic turnover; [ 28 ]). The implications of the electrochemical reversibility are that NADH:flavin oxidoreduction is kinetically fast and thermodynamically efficient. electron transport chain enzyme mechanism mitochondrion NADH:quinone oxidoreductase proton-coupled electron transfer proton...

... complex I electron transport chain mitochondrion NADH:ubiquinone oxidoreductase Pichia angusta Pichia pastoris 1 To whom correspondence should be addressed (email jh@mrc-mbu.cam.ac.uk ). 26 3 2009 21 5 2009 22 5 2009 22 5 2009 In mitochondria...

... Society 2008 complex I electron transport chain H 2 O 2 mitochondria oxaloacetate superoxide Mitochondria and ROS (reactive oxygen species) have emerged as central players in a wide variety of pathologies. Elevation in mitochondrial ROS production has been hypothesized to occur...

...Steven Sherwood; Judy Hirst Complex I (NADH:ubiquinone oxidoreductase) is the first enzyme of the membrane-bound electron transport chain in mitochondria. It conserves energy, from the reduction of ubiquinone by NADH, as a protonmotive force across the inner membrane, but the mechanism of energy...

... (electron transport chain) to molecular oxygen. Complexes I and III produce most of the O 2 •− , which is detoxified by MnSOD (where SOD stands for superoxide dismutase) or cytosolic Cu/ZnSOD to produce H 2 O 2 . Mitochondria also strongly depend on GSH to combat the oxidative stress; for example, H 2 O 2...

.... Over the course of the last 7 years, it has become apparent that the rate of superoxide production by the electron transport chain in vitro is sensitive to the mitochondrial protonmotive force (Δp) [ 21 , 22 , 24 , 25 ]. This conclusion is based on observations that addition of either uncouplers...

... by rotenone, a complex I inhibitor, as well as other chemical inhibitors of electron flow that act further downstream in the electron transport chain. The effects of cytochrome c depletion from mitoplasts on ROS production and respiration are reversible upon addition of exogenous cytochrome c . Thus...