...Tessa R. Young; Zhiguang Xiao Metal ions play many critical roles in biology, as structural and catalytic cofactors, and as cell regulatory and signalling elements. The metal–protein affinity, expressed conveniently by the metal dissociation constant, K D , describes the thermodynamic strength...

... issues related with extracellular electron transfer. Using NMR to probe protein–protein interactions, kinetic measurements of electron transfer and electrostatic calculations, we were able to identify protein partners and their docking sites, and determine the dissociation constants. The results showed...

... compilation © 2010 Biochemical Society 2010 common β-subunit (βc) cytokine receptor cytokine-receptor homology module (CRM) dissociation constant granulocyte/macrophage colony-stimulating factor (GM-CSF) immunoglobulin-like domain interleukin-3 (IL-3) interleukin-5 (IL-5) GM-CSF...

... by ellipsometric measurement of protein depletion from the supernatant following incubation with platelets. At low ionic strength (20 mM NaCl, no CaCl 2 ), a dissociation constant ( K d ) of 0.2 μM was found for β 2 GPI, with 7.4×10 5 binding sites per platelet. Under physiologically relevant conditions (120 mM...

... of NtCBK2 has been localized in a stretch of 30 amino acid residues at residue positions 431–460 as a 1-5-10 protein motif. Three tobacco CaM isoforms (NtCaM1, NtCaM3 and NtCaM13) used in the present study have been shown to bind to NtCBK2, but with different dissociation constants ( K d s), as follows...

... into their respective acyl-CoA esters. 1 These authors have contributed equally to the work. 2 To whom correspondence should be addressed (e-mail jkk@bmb.sdu.dk ). 26 11 2001 20 2 2002 23 4 2002 The Biochemical Society, London ©2002 2002 diagnostics dissociation constant...

... demonstrate that the conversion of Tyr-992 into glutamate induces a 4-fold decrease in the EGFr apparent low-affinity dissociation constant, whereas the mutation of two acidic residues, Asp-988 and Glu-991, or the conversion of Tyr-992 into phenylalanine does not alter EGFr affinity. Phorbol ester treatment...

... utilizing a solid-phase binding assay and estimated the dissociation constants for the interaction of various apoE and Aβ species. Using native apoE isoforms from stably transfected RAW 264 and human embryonic kidney 293 cells, apoE3 had greater affinity than apoE4 for both Aβ1-40 and Aβ1-42. Delipidation...

... involve a change in environment around one or more tyrosine residues. Key words: dissociation constant, fluorescence, mutagenesis, stopped-flow kinetics, tryptophan. The structure of a single Ig-binding domain of protein L (PpL) from strain 312 has been resolved by "H NMR [10,11]. It reveals a domain...

... site may strengthen this interaction. 1 To whom correspondence should be addressed (e-mail Leslie.Gold@ccmail.med.nyu.edu ). 13 5 1998 2 11 1998 8 12 1998 The Biochemical Society, London © 1999 1999 dissociation constant ELISA fibrin clot thrombus wound healing...