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Keywords: dissociation constantClose
Biochem J (2021) 478 (5): 1085–1116.
Published: 10 March 2021
...Tessa R. Young; Zhiguang Xiao Metal ions play many critical roles in biology, as structural and catalytic cofactors, and as cell regulatory and signalling elements. The metal–protein affinity, expressed conveniently by the metal dissociation constant, K D , describes the thermodynamic strength...
Bruno M. Fonseca, Catarina M. Paquete, Sónia E. Neto, Isabel Pacheco, Cláudio M. Soares, Ricardo O. Louro
Biochem J (2013) 449 (1): 101–108.
Published: 07 December 2012
... issues related with extracellular electron transfer. Using NMR to probe protein–protein interactions, kinetic measurements of electron transfer and electrostatic calculations, we were able to identify protein partners and their docking sites, and determine the dissociation constants. The results showed...
Includes: Multimedia, Supplementary data
Biochem J (2010) 426 (3): 307–317.
Published: 24 February 2010
... compilation © 2010 Biochemical Society 2010 common β-subunit (βc) cytokine receptor cytokine-receptor homology module (CRM) dissociation constant granulocyte/macrophage colony-stimulating factor (GM-CSF) immunoglobulin-like domain interleukin-3 (IL-3) interleukin-5 (IL-5) GM-CSF...
Includes: Supplementary data
Edouard M. BEVERS, Marie P. JANSSEN, Paul COMFURIUS, Krishnakumar BALASUBRAMANIAN, Alan J. SCHROIT, Robert F. A. ZWAAL, George M. WILLEMS
Biochem J (2005) 386 (2): 271–279.
Published: 22 February 2005
... by ellipsometric measurement of protein depletion from the supernatant following incubation with platelets. At low ionic strength (20 mM NaCl, no CaCl 2 ), a dissociation constant ( K d ) of 0.2 μM was found for β 2 GPI, with 7.4×10 5 binding sites per platelet. Under physiologically relevant conditions (120 mM...
Biochem J (2003) 376 (1): 291–302.
Published: 15 November 2003
... of NtCBK2 has been localized in a stretch of 30 amino acid residues at residue positions 431–460 as a 1-5-10 protein motif. Three tobacco CaM isoforms (NtCaM1, NtCaM3 and NtCaM13) used in the present study have been shown to bind to NtCBK2, but with different dissociation constants ( K d s), as follows...
Majken C.T. WADUM, Jens K. VILLADSEN, Søren FEDDERSEN, Rikke S. MØLLER, Thomas B.F. NEERGAARD, Birthe B. KRAGELUND, Peter HØJRUP, Nils J. FÆRGEMAN, Jens KNUDSEN
Biochem J (2002) 365 (1): 165–172.
Published: 01 July 2002
... into their respective acyl-CoA esters. 1 These authors have contributed equally to the work. 2 To whom correspondence should be addressed (e-mail email@example.com ). 26 11 2001 20 2 2002 23 4 2002 The Biochemical Society, London ©2002 2002 diagnostics dissociation constant...
Biochem J (2000) 352 (1): 99–108.
Published: 07 November 2000
... demonstrate that the conversion of Tyr-992 into glutamate induces a 4-fold decrease in the EGFr apparent low-affinity dissociation constant, whereas the mutation of two acidic residues, Asp-988 and Glu-991, or the conversion of Tyr-992 into phenylalanine does not alter EGFr affinity. Phorbol ester treatment...
Takahiko TOKUDA, Miguel CALERO, Etsuro MATSUBARA, Ruben VIDAL, Asok KUMAR, Bruno PERMANNE, Berislav ZLOKOVIC, Jonathan D. SMITH, Mary Jo LADU, Agueda ROSTAGNO, Blas FRANGIONE, Jorge GHISO
Biochem J (2000) 348 (2): 359–365.
Published: 23 May 2000
... utilizing a solid-phase binding assay and estimated the dissociation constants for the interaction of various apoE and Aβ species. Using native apoE isoforms from stably transfected RAW 264 and human embryonic kidney 293 cells, apoE3 had greater affinity than apoE4 for both Aβ1-40 and Aβ1-42. Delipidation...
Biochem J (1999) 340 (1): 193–199.
Published: 10 May 1999
... involve a change in environment around one or more tyrosine residues. Key words: dissociation constant, fluorescence, mutagenesis, stopped-flow kinetics, tryptophan. The structure of a single Ig-binding domain of protein L (PpL) from strain 312 has been resolved by "H NMR [10,11]. It reveals a domain...
Biochem J (1999) 338 (2): 375–386.
Published: 22 February 1999
... site may strengthen this interaction. 1 To whom correspondence should be addressed (e-mail Leslie.Gold@ccmail.med.nyu.edu ). 13 5 1998 2 11 1998 8 12 1998 The Biochemical Society, London © 1999 1999 dissociation constant ELISA fibrin clot thrombus wound healing...