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Keywords: crystal structure
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Biochem J (2022) 479 (18): 1999–2011.
Published: 30 September 2022
... performed, and repeated experiments showed similar results. Representative results are presented. aggregation amyloidosis crystal structure stability transthyretin Amyloidoses are a group of diseases in which abnormal proteins called amyloid fibrils cause functional impairment of organs...
Includes: Supplementary data
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Biochem J (2020) 477 (23): 4623–4634.
Published: 11 December 2020
.... FRMPD4 is a neural scaffold protein that binds to metabotropic glutamate receptors via its FERM domain. Here, we determine the crystal structure of the FERM domain of FRMPD4 at 2.49 Å resolution. The structure reveals that the canonical target binding groove of FRMPD4 FERM is occupied by a conserved...
Includes: Supplementary data
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Biochem J (2018) 475 (21): 3393–3416.
Published: 09 November 2018
... in multidrug resistance and virulence of prokaryotes. The binding of cadmium, zinc, and several other metals to Rv3488 was discovered and characterized by isothermal titration calorimetery to be an exothermic process. Crystal structures of apo-Rv3488 and Rv3488 in complex with cadmium or zinc ions were...
Includes: Supplementary data
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Biochem J (2018) 475 (16): 2667–2679.
Published: 31 August 2018
... of ALT cell viability. Here, we present the crystal structure of the NuRD subunit RBBP4 bound to the N-terminal 14 amino acids of ZNF827. RBBP4 forms a negatively charged channel that binds to ZNF827 through a network of electrostatic interactions. We identify the precise amino acids in RBBP4 required...
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Biochem J (2018) 475 (16): 2681–2697.
Published: 31 August 2018
... are similar to one of the two types of their bacterial counterparts, the long-type ATP-PRTs. A biochemical and structural study of ATP-PRT from the model legume plant, Medicago truncatula ( Medtr ATP-PRT1) is reported herein. Two crystal structures, presenting homohexameric Medtr ATP-PRT1 in its relaxed (R...
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Biochem J (2018) 475 (6): 1107–1119.
Published: 20 March 2018
... step of the aspartate family amino acid biosynthesis. Owing to various structural organizations, AKs from different species show tremendous diversity and complex allosteric controls. We report the crystal structure of AK from Pseudomonas aeruginosa (PaAK), a typical α2β2 hetero-tetrameric enzyme...
Includes: Supplementary data
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Biochem J (2018) 475 (3): 547–560.
Published: 09 February 2018
... of the Biochemical Society 2018 crystal structure cytarabine peptidyl-tRNA hydrolase The process of protein synthesis in a normal healthy cell involves translation of genetic information contained in mRNA into a polypeptide chain. However, due to various reasons, the translation process often gets...
Includes: Supplementary data
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Biochem J (2018) 475 (2): 415–428.
Published: 23 January 2018
... of the chiX gene in S. marcescens led to a mutant strain blocked for secretion of all members of the chitinolytic machinery. In this work, a detailed structural and biochemical characterisation of ChiX is presented. The high-resolution crystal structure of ChiX reveals the protein to be a member of the LAS...
Includes: Supplementary data
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Biochem J (2017) 474 (22): 3705–3717.
Published: 01 November 2017
.... The 2.0 Å X-ray crystal structure of PpCM1 in complex with the tryptophan identified the allosteric effector site and reveals structural differences between the R- (more active) and T-state (less active) forms of plant chorismate mutases. Molecular insight into the basal plant chorismate mutases guides...
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Biochem J (2017) 474 (20): 3373–3389.
Published: 26 September 2017
... endo-hydrolysis of the β-1,4-glycosidic linkage adjacent to a 3- O -substitued glucose residue in the mixed-linked β-glucans. The crystal structure of F32EG5 was determined to 2.8 Å resolution, and the crystal structure of the complex of F32EG5 E193Q mutant and cellotetraose was determined to 1.7...
Includes: Supplementary data
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Biochem J (2016) 473 (18): 2863–2880.
Published: 12 September 2016
... construct (residues 255–299) with VBM deletion abrogated p97 interaction ( Figure 1A–C ). Subsequently, we used a 15 amino acid synthesized peptide comprising an RHBDL4 VBM (residues 300–314) to produce a complex crystal with p97N. The crystal structure of p97N–VBM complex was determined at 1.88...
Includes: Multimedia, Supplementary data
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Biochem J (2016) 473 (15): 2395–2411.
Published: 28 July 2016
... use. © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 coagulation Factor IX conformational changes crystal structure enzyme kinetics substrate-binding site thermal shift assay Blood coagulation serves to react on vessel...
Includes: Supplementary data
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Biochem J (2016) 473 (8): 1085–1095.
Published: 08 April 2016
... perturbations in 1 H- 15 N-HSQC resonances, the chitosan tetramer (GlcN) 4 was found to bind to the loop region extruded from the core β-sandwich of DD1 and DD2. The binding site defined by NMR in solution was consistent with the crystal structure of DD2 in complex with (GlcN) 3 , in which the bound (GlcN) 3...
Includes: Supplementary data
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Biochem J (2016) 473 (4): 365–370.
Published: 09 February 2016
.... anion transporters crystal structure electromotility prestin SLC26 STAS domain In order to obtain insights into the anion-binding properties of prestin, a member of the SLC26 (solute carrier 26) family of anion transporters, we determined crystal structures of a mammalian STAS (sulfate...
Includes: Supplementary data
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Biochem J (2015) 472 (3): 297–307.
Published: 27 November 2015
.... In the present study, we present novel crystal structures of the dimeric Cc AAOR in complex with the cofactor and glycerol, D -xylose, D -glucose, maltotriose and D -sorbitol determined to resolutions of 2.0, 1.8, 1.7, 1.9 and 1.8 Å (1 Å=0.1 nm), respectively. These complex structures allowed for a detailed...
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Biochem J (2015) 469 (3): 367–374.
Published: 23 July 2015
... that the mechanisms of cyclic-di-AMP (c-di-AMP) synthesis and its inhibition are conserved among different DAC enzymes and bacterial species. crystal structure cyclic-di-adenosine 5′-monophos-phate (c-di-AMP) diadenylate cyclase (DAC) domain inhibition reaction mechanism Nucleotide second messengers...
Includes: Supplementary data
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Biochem J (2015) 469 (1): 145–158.
Published: 19 June 2015
... with low sequence identity (<24%) to the previously reported GH31 glycosidases and characterized two enzymes from Pedobacter heparinus and Pedobacter saltans . The enzymes unexpectedly exhibited α-galactosidase activity, but were not active on α-glucosides and α-xylosides. The crystal structures of one...
Includes: Supplementary data
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Biochem J (2015) 468 (3): 475–484.
Published: 15 June 2015
... to date have focused primarily on the class II KARIs, which arose through domain duplication. In the present study, we present five new crystal structures of class I KARIs. These include the first structure of a KARI with a six-residue β2αB (cofactor specificity determining) loop and an NADPH phosphate...
Includes: Supplementary data
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Biochem J (2015) 467 (3): 529–536.
Published: 17 April 2015
...Mark W. Richards; Laura O'Regan; Daniel Roth; Jessica M. Montgomery; Anne Straube; Andrew M. Fry; Richard Bayliss We present crystal structures of a trimeric coiled-coil domain found in echinoderm microtubule (MT)-associated protein (EMAP)-like (EML) proteins. This trimerization domain (TD...
Includes: Supplementary data
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Biochem J (2015) 467 (1): 103–113.
Published: 20 March 2015
... stabilization and molecular mechanism for substrate recognition of αTAT1 © The Authors Journal compilation © 2015 Biochemical Society 2015 1 To whom correspondence should be addressed (email hsumi@med.kyushu-u.ac.jp ). Crystal structures of the αTAT domain of MEC-17 in complex with acetoacetyl...
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Biochem J (2014) 463 (3): 319–328.
Published: 10 October 2014
.... In the present paper we describe the crystal structure of the complex between human Ca 2+ /CaM and the CaM-binding region of human IP 3 -3K isoform A (residues 158–183) and propose a model for a complex including the kinase domain. The structure obtained allowed us to identify all of the key residues involved...
Includes: Supplementary data
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Biochem J (2014) 461 (2): 247–255.
Published: 26 June 2014
... from subtle differences in protein structure. We solved the crystal structure of Npu DnaE intein in the split form. This is the first trans -structure of split inteins. Guided by this structure, we identified two residues, N Arg 50 and C Ser 35 , which play a significant role in modulating...
Includes: Supplementary data
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Biochem J (2013) 454 (2): 191–200.
Published: 09 August 2013
... APH(3′)-Ia antibiotic resistance crystal structure protein kinase inhibitor structure-based drug design Antibiotic resistance is a serious global public health problem [ 1 ]. Resistance is an issue with all classes of currently used antibiotics, resulting in the emergence of pathogens...
Includes: Supplementary data
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Biochem J (2013) 454 (2): 311–321.
Published: 09 August 2013
...Steven M. Sine; Sun Huang; Shu-Xing Li; Corrie J. B. daCosta; Lin Chen The crystal structure of a pentameric α7 ligand-binding domain chimaera with bound α-btx (α-bungarotoxin) showed that of the five conserved aromatic residues in α7, only Tyr 184 in loop C of the ligand-binding site was required...
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Biochem J (2013) 454 (2): 303–310.
Published: 09 August 2013
...Sun Huang; Shu-Xing Li; Nina Bren; Kevin Cheng; Ryan Gomoto; Lin Chen; Steven M. Sine To identify high-affinity interactions between long-chain α-neurotoxins and nicotinic receptors, we determined the crystal structure of the complex between α-btx (α-bungarotoxin) and a pentameric ligand-binding...
Includes: Supplementary data
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Biochem J (2013) 451 (1): 33–44.
Published: 14 March 2013
... determined the crystal structure of PKCθC1B to 1.63 Å (1 Å=0.1 nm) resolution, which showed that Trp 253 at the rim of the activator-binding pocket was orientated towards the membrane, whereas in PKCδC1B the homologous tryptophan residue was orientated away from the membrane. This particular orientation...
Includes: Supplementary data
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Biochem J (2013) 450 (1): 95–105.
Published: 24 January 2013
... and it is therefore important to understand the structural basis of specific filament assembly. However, three-dimensional structures are currently available for only three of the four subgroups. In the present study we describe the crystal structure of a construct of human SEPT3 which belongs to the outstanding...
Includes: Supplementary data
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Biochem J (2013) 449 (1): 161–166.
Published: 07 December 2012
... an irreversible process. In the present study, we demonstrate that an active enzyme can be converted back into its zymogen form. We determined the crystal structure of uPA (urokinase-type plasminogen activator) in complex with an inhibitory antibody, revealing that the antibody ‘rezymogenizes’ already activated...
Includes: Supplementary data
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Biochem J (2012) 448 (3): 401–407.
Published: 21 November 2012
... crystal structures of XylC in complex with Tris, xylobiose and xylose at 1.48–2.05 Å (1 Å=0.1 nm) resolution. XylC assembles into a tetramer, and each monomer comprises two distinct domains. The core domain is a right-handed parallel β-helix (residues 1–75 and 201–638) and the flanking region (residues 76...
Includes: Supplementary data
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Biochem J (2012) 448 (3): 321–328.
Published: 21 November 2012
... of the mitotic kinases Bub1 (budding uninhibited by benzamidine 1), BubR1 (Bub1 related) and Mps1 (monopolar spindle 1), which is mediated by the N-terminus of each kinase. In the present study we report the 1.8 Å (1 Å=0.1 nm) crystal structure of the TPR (tetratricopeptide repeat) domain in the N-terminal...
Includes: Supplementary data
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Biochem J (2012) 444 (3): 445–455.
Published: 29 May 2012
... proteins, none of which have been experimentally characterized. We have found that two P. aeruginosa hotdog proteins display high thioesterase activity against 3-hydroxy-3-methylglutaryl-CoA and glutaryl-CoA (PA5202), and octanoyl-CoA (PA2801). Crystal structures of these proteins were solved (at 1.70...
Includes: Supplementary data
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