.... Sheppard; John C. Sembrat; Mauricio Rojas; Charles F. McTiernan; Paul J. Barton; Peter H. Sugden The extracellular signal-regulated kinase 1/2 (ERK1/2) cascade promotes cardiomyocyte hypertrophy and is cardioprotective, with the three RAF kinases forming a node for signal integration. Our aims were...

...Kristina K. Durham; Kevin M. Chathely; Bernardo L. Trigatti The cardioprotective lipoprotein HDL (high-density lipoprotein) prevents myocardial infarction and cardiomyocyte death due to ischemia/reperfusion injury. The scavenger receptor class B, type 1 (SR-B1) is a high-affinity HDL receptor...

.... cruzi invasion in cardiomyocytes elicits the production of pro-inflammatory mediators [TNF-α, IL-1β, IFN-γ; nitric oxide ( · NO)], leading to mitochondrial dysfunction with increased superoxide radical (O 2 ·− ), hydrogen peroxide (H 2 O 2 ) and peroxynitrite generation. We hypothesize that these redox...

...Jordan J. Bartlett; Purvi C. Trivedi; Pollen Yeung; Petra C. Kienesberger; Thomas Pulinilkunnil Doxorubicin (DOX) is an effective anti-cancer agent. However, DOX treatment increases patient susceptibility to dilated cardiomyopathy. DOX predisposes cardiomyocytes to insult by suppressing...

.... We also find that both mutants have reduced F-actin binding affinity, although the differences are not significant. The full length mEos2 tagged protein expressed in adult cardiomyocytes shows that both mutations additionally affect Z-disc localization and dynamic behaviour. Overall, these two...

.... Nevertheless, the relationships among diabetes, hyperglycaemia, mitochondrial dysfunction and O-GlcNAc modifications remain unclear. In the present study, we tested whether high-glucose-induced increases in O-GlcNAc modifications directly regulate mitochondrial function in isolated cardiomyocytes. Augmentation...

...Elisabet Sarri; David Garcia-Dorado; Arancha Abellan; Jordi Soler-Soler A decrease in [ 3 H]Cho (choline) incorporation in to PtdCho (phos-phatidylcholine) preceded the onset of LDH (lactate dehydrogenase) release in HL-1 cardiomyocytes submitted to simulated ischaemia. This observation led us...

... rat ventricular cardiomyocytes were electrically stimulated to perform rhythmic contractions to permit the determination of potential sites of convergence of contraction and insulin signalling to glucose transport. The participation of phosphoinositide 3-kinase (PI-3K) in Ca 2+ - and contraction...

... conclude that AE3 and AE3c can contribute to pH i recovery after cellular-acid loading. Key words: anion exchange, cardiomyocytes, cell acidosis, ischaemia, reperfusion. junction conductance and, in turn, cardiac-cell electrical coupling [8,13]. During a cardiac ischaemic episode, heart cells develop...

.... An 18-mer antisense phosphorothioate oligodeoxynucleotide was used to target a translated region of cyclophilin-A mRNA in rat neonatal cardiomyocytes. After a 24 h exposure to the oligonucleotide, the amount of cyclophilin-A in the cells was decreased by at least 93% as judged by immunological...